3.5.4.33 adenine34 in tRNA + H2O - Saccharomyces cerevisiae hypoxanthine34 in tRNA + NH3 - ? 419330 3.5.4.33 adenine34 in tRNA + H2O enzyme accounts for the presence of inosine 34 in all seven cytoplasmic A34-containing precursor tRNAs in yeast Saccharomyces cerevisiae hypoxanthine34 in tRNA + NH3 - ? 419330 3.5.4.33 adenine34 in tRNA + H2O the efficacy of adenosine 34 to inosine 34 conversion depends on the nucleotide sequence of the anticodon loop and its proximal stem, the best tRNA substrates being those with a purine at position 35. The enzyme accounts for the presence of inosine 34 in all seven cytoplasmic A34-containing precursor tRNAs in yeast Saccharomyces cerevisiae hypoxanthine34 in tRNA + NH3 - ? 419330 3.5.4.33 adenine34 in tRNA + H2O enzyme accounts for the presence of inosine 34 in all seven cytoplasmic A34-containing precursor tRNAs in yeast Saccharomyces cerevisiae ATCC 204508 hypoxanthine34 in tRNA + NH3 - ? 419330 3.5.4.33 adenine34 in tRNA + H2O the efficacy of adenosine 34 to inosine 34 conversion depends on the nucleotide sequence of the anticodon loop and its proximal stem, the best tRNA substrates being those with a purine at position 35. The enzyme accounts for the presence of inosine 34 in all seven cytoplasmic A34-containing precursor tRNAs in yeast Saccharomyces cerevisiae ATCC 204508 hypoxanthine34 in tRNA + NH3 - ? 419330 3.5.4.33 adenine34 in tRNA + H2O - Saccharomyces cerevisiae ATCC 204508 hypoxanthine34 in tRNA + NH3 - ? 419330 3.5.4.33 adenine34 in tRNAAla(AGC) + H2O - Saccharomyces cerevisiae hypoxanthine34 in tRNAAla(IGC) + NH3 - ? 420237 3.5.4.33 adenine34 in tRNAAla(AGC) + H2O - Saccharomyces cerevisiae ATCC 204508 hypoxanthine34 in tRNAAla(IGC) + NH3 - ? 420237 3.5.4.33 adenine34 in tRNAArg + H2O - Escherichia coli hypoxanthine34 in tRNAArg + NH3 - ? 419331 3.5.4.33 adenine34 in tRNAArg + H2O - Streptococcus pyogenes hypoxanthine34 in tRNAArg + NH3 - ? 419331 3.5.4.33 adenine34 in tRNAArg + H2O - Agrobacterium tumefaciens hypoxanthine34 in tRNAArg + NH3 - ? 419331 3.5.4.33 adenine34 in tRNAArg + H2O - Aquifex aeolicus hypoxanthine34 in tRNAArg + NH3 - ? 419331 3.5.4.33 adenine34 in tRNAArg + H2O - Arabidopsis thaliana hypoxanthine34 in tRNAArg + NH3 - ? 419331 3.5.4.33 adenine34 in tRNAArg + H2O - Saccharomyces cerevisiae hypoxanthine34 in tRNAArg + NH3 - ? 419331 3.5.4.33 adenine34 in tRNAArg + H2O the chloroplast tRNA editing protein is specifically required for deamination of chloroplast (cp)-tRNAArg(ACG) to cp-tRNAArg(ICG) Arabidopsis thaliana hypoxanthine34 in tRNAArg + NH3 - ? 419331 3.5.4.33 adenine34 in tRNAArg + H2O the enzyme catalyzes the site-specific inosine formation at the wobble position (position 34) of tRNAArg2, the only tRNA having this modification in prokaryotes. tadA is an essential gene in Escherichiy coli Escherichia coli hypoxanthine34 in tRNAArg + NH3 - ? 419331 3.5.4.33 adenine34 in tRNAArg + H2O complementation studies show that the C-terminal domain is sufficient for cognate tRNA deamination both in vitro and in planta. TADA is exclusively involved in editing of the prokaryote-type cp-tRNAArg(ACG). No editing of cytosolic tRNAAla(AGC), tRNAVal(AAC), and tRNAThr(AGU). TADA is also not involved in editing of nucleotide A37 of tRNAAla(AGC) Arabidopsis thaliana hypoxanthine34 in tRNAArg + NH3 - ? 419331 3.5.4.33 adenine34 in tRNAArg + H2O Escherichia coli tRNAArg2. A critical role in tRNA substrate recognition is proposed for the conserved FFxxxR motif at the C-terminus (unique to tadA) Escherichia coli hypoxanthine34 in tRNAArg + NH3 - ? 419331 3.5.4.33 adenine34 in tRNAArg + H2O Escherichia coli tRNAArg2. A critical role in tRNA substrate recognition is proposed for the conserved FFxxxR motif at the C-terminus (unique to tadA) Aquifex aeolicus hypoxanthine34 in tRNAArg + NH3 - ? 419331 3.5.4.33 adenine34 in tRNAArg + H2O Escherichia coli tRNAArg2. A critical role in tRNA substrate recognition is proposed for the conserved FFxxxR motif at the C-terminus (unique to tadA) Agrobacterium tumefaciens hypoxanthine34 in tRNAArg + NH3 - ? 419331 3.5.4.33 adenine34 in tRNAArg + H2O Escherichia coli tRNAArg2. A critical role in tRNA substrate recognition is proposed for the conserved FFxxxR motif at the C-terminus (unique to tadA) Saccharomyces cerevisiae hypoxanthine34 in tRNAArg + NH3 - ? 419331 3.5.4.33 adenine34 in tRNAArg + H2O kinetic isotope effects and quantum molecular modeling are applied to solve the transition state structure of the enzyme. The key events of the tRNA editing process are facilitated by the enzyme-promoted base flip and the Glu70-mediated proton shuttle Escherichia coli hypoxanthine34 in tRNAArg + NH3 - ? 419331 3.5.4.33 adenine34 in tRNAArg + H2O the enzyme catalyzes the site-specific inosine formation at the wobble position (position 34) of tRNAArg2, the only tRNA having this modification in prokaryotes. With the exception of yeast tRNAArg, no other eukaryotic tRNA substrates are found to be modified by tadA. An artificial yeast tRNAAsp, which carries the anticodon loop of yeast tRNAArg, is bound and modified by tadA. A tRNAArg2 minisubstrate containing the anticodon stem and loop is sufficient for specific deamination by tadA. Nucleotides at positions 33-36 are sufficient for inosine formation in mutant Arg2 minisubstrates. The anticodon is thus a major determinant for tadA substrate specificity Escherichia coli hypoxanthine34 in tRNAArg + NH3 - ? 419331 3.5.4.33 adenine34 in tRNAArg + H2O - Saccharomyces cerevisiae ATCC 204508 hypoxanthine34 in tRNAArg + NH3 - ? 419331 3.5.4.33 adenine34 in tRNAArg + H2O Escherichia coli tRNAArg2. A critical role in tRNA substrate recognition is proposed for the conserved FFxxxR motif at the C-terminus (unique to tadA) Saccharomyces cerevisiae ATCC 204508 hypoxanthine34 in tRNAArg + NH3 - ? 419331 3.5.4.33 adenine34 in tRNAArg2 + H2O - Aquifex aeolicus hypoxanthine34 in tRNAArg2 + NH3 - ? 419332 3.5.4.33 adenine34 in tRNAArg2 + H2O the bacterial tRNA adenosine deaminase generates inosine by deaminating the adenosine residue at the wobble position of tRNAArg-2. This modification is essential for the decoding system Aquifex aeolicus hypoxanthine34 in tRNAArg2 + NH3 - ? 419332 3.5.4.33 adenine34 in tRNASer(AGA) + H2O - Saccharomyces cerevisiae hypoxanthine34 in tRNASer(IGA) + NH3 - ? 420238 3.5.4.33 adenine34 in tRNASer(AGA) + H2O - Saccharomyces cerevisiae ATCC 204508 hypoxanthine34 in tRNASer(IGA) + NH3 - ? 420238 3.5.4.33 adenine34 in tRNAThr(AGU) + H2O - Trypanosoma brucei hypoxanthine34 in tRNAThr(IGU) + NH3 - ? 419333 3.5.4.33 adenine34 in tRNAThr(AGU) + H2O in vitro, this enzyme can mediate A-to-I editing of tRNA and C-to-U deamination of ssDNA Trypanosoma brucei hypoxanthine34 in tRNAThr(IGU) + NH3 - ? 419333 3.5.4.33 adenine34 in tRNAVal + H2O - Trypanosoma brucei hypoxanthine34 in tRNAVal + NH3 - ? 419334