3.5.1.78	gamma-Glu-Ala-Gly-4-nitroanilide + H2O	-	Escherichia coli	?	-	?	17773	
3.5.1.78	glutathionylspermidine + H2O	-	Escherichia coli	glutathione + spermidine	-	?	17772	
3.5.1.78	glutathionylspermidine + H2O	-	Crithidia fasciculata	glutathione + spermidine	-	r	17772	
3.5.1.78	glutathionylspermidine + H2O	-	Escherichia coli K-12	glutathione + spermidine	-	?	17772	
3.5.1.78	glutathionylspermidine + H2O	-	Escherichia coli	glutathione + spermidine	GspSA catalyzes the formation of a peptide bond between GSH and spermidine, cf. EC 6.3.1.8	r	17772	
3.5.1.78	glutathionylspermidine + H2O	the N-terminal domain catalyzes the hydrolysis of the conjugate	Escherichia coli	glutathione + spermidine	GspSA catalyzes the formation of a peptide bond between GSH and spermidine, cf. EC 6.3.1.8	r	17772	
3.5.1.78	glutathionylspermidine disulfide + H2O	a coupled reaction of Gsp amidase and glutathione reductase	Escherichia coli K-12	glutathione + ?	products of the coupled reaction of Gsp amidase and glutathione reductase: spermidine, glutathionylspermidine-glutathione mixed disulfide, and glutathione disulfide are generated	?	414655	
3.5.1.78	additional information	glutathionylspermidine S-thiolated peptides and proteins are tested as substrates. The protein substrates are treated with disulfide or biotinated glutathionylspermidine to generate biotin-labeled glutathionylspermidine S-thiolated proteins for the detection with an anti-biotin antibody after the reaction with the GspSA. A variety of glutathionylspermidine-derivatized substrates are hydrolyzed yielding spermidine and glutathione S-thiolated protein/peptides	Escherichia coli K-12	?	-	?	89	
3.5.1.78	additional information	bifunctional enzyme that catalyzes the ATP-dependent formation and hydrolysis of glutathionylspermidine, a conjugate of glutathione and spermidine. Proteins YgiC and YjfC, encoded by genes ygiC and yjfC, show ATPase activity, but are not glutathionylspermidine synthetases	Escherichia coli	?	-	?	89	
3.5.1.78	trypanothione + H2O	-	Leishmania major	glutathione + glutathionylspermidine	-	?	367840	
3.5.1.78	trypanothione + H2O	hydrolysis at 1.5% the rate of glutathionylspermidine	Crithidia fasciculata	glutathione + glutathionylspermidine	-	?	367840