3.4.25.1 1-aminocyclopropane-1-carboxylic acid synthase 6 + H2O phosphorylation of 1-aminocyclopropane-1-carboxylic acid synthase 6 introduces negative charges to the C-terminus of ACS6, which reduces the turnover of 1-aminocyclopropane-1-carboxylic acid synthase 6 by the 26S proteasome degradation machinery Arabidopsis thaliana ? - ? 395226 3.4.25.1 4-hydroxy-3-nitrophenol-Leu-Leu-Asn-vinylsulfone + H2O - Trypanosoma brucei ? - ? 367784 3.4.25.1 4-hydroxy-3-nitrophenyl-Leu-Leu-Lys-vinylsulfone + H2O - Trypanosoma brucei ? - ? 367782 3.4.25.1 AAF-7-amido-4-methylcoumarin + H2O highest activity in testicle, kidney and brain Rattus norvegicus ? - ? 367759 3.4.25.1 ABZ-Val-Val-Ser-Arg-Ser-Leu-Gly-Tyr(3-NO2)-NH2 + H2O synthetic, internally quenched substrate Homo sapiens ? - ? 449469 3.4.25.1 acetyl-Ala-Pro-norleucine-Leu-Leu-7-amido-4-methylcoumarin + H2O - Saccharomyces cerevisiae acetyl-Ala-Pro-norleucine-Leu-Leu + 7-amino-4-methylcoumarin - ? 367793 3.4.25.1 acetyl-Ala-Pro-norleucine-Leu-Leu-7-amido-4-methylcoumarin + H2O - Oryctolagus cuniculus acetyl-Ala-Pro-norleucine-Leu-Leu + 7-amino-4-methylcoumarin - ? 367793 3.4.25.1 acetyl-Arg-hSer-Thr-Arg-7-amido-4-methylcoumarin + H2O substrate for trypsin-like activity Homo sapiens acetyl-Arg-hSer-Thr-Arg + 7-amino-4-methylcoumarin - ? 449495 3.4.25.1 acetyl-betaAla-Met(sulfone)-Thr-Arg-7-amido-4-methylcoumarin + H2O substrate for trypsin-like activity Homo sapiens acetyl-betaAla-Met(sulfone)-Thr-Arg + 7-amino-4-methylcoumarin - ? 449496 3.4.25.1 acetyl-DPSD-7-amido-4-methylcoumarin + H2O cleaved by the beta1 subunit of the 20S proteasome Homo sapiens ? - ? 396332 3.4.25.1 acetyl-EPFD-7-amido-4-carbamoylcoumarin + H2O - Homo sapiens ? - ? 367785 3.4.25.1 acetyl-EPFD-7-amido-4-carbamoylcoumarin + H2O - Trypanosoma brucei ? - ? 367785 3.4.25.1 acetyl-GPLD-7-amido-4-methylcoumarin + H2O - Saccharomyces cerevisiae acetyl-GPLD + 7-amino-4-methylcoumarin - ? 367790 3.4.25.1 acetyl-GPLD-7-amido-4-methylcoumarin + H2O - Oryctolagus cuniculus acetyl-GPLD + 7-amino-4-methylcoumarin - ? 367790 3.4.25.1 acetyl-GPLE-7-amido-4-methylcoumarin + H2O - Saccharomyces cerevisiae acetyl-GPLE + 7-amino-4-methylcoumarin - ? 367791 3.4.25.1 acetyl-GPLE-7-amido-4-methylcoumarin + H2O - Oryctolagus cuniculus acetyl-GPLE + 7-amino-4-methylcoumarin - ? 367791 3.4.25.1 acetyl-GPLL-7-amido-4-methylcoumarin + H2O - Saccharomyces cerevisiae acetyl-GPLL + 7-amino-4-methylcoumarin - ? 367792 3.4.25.1 acetyl-GPLL-7-amido-4-methylcoumarin + H2O - Oryctolagus cuniculus acetyl-GPLL + 7-amino-4-methylcoumarin - ? 367792 3.4.25.1 acetyl-hArg-Pro-2-fluoro-Phe-Asp-7-amido-4-methylcoumarin + H2O substrate for caspase-like activity Homo sapiens acetyl-hArg-Pro-2-fluoro-Phe-Asp + 7-amino-4-methylcoumarin - ? 449506 3.4.25.1 acetyl-hArg-Pro-Abu-Asp-7-amido-4-methylcoumarin + H2O substrate for caspase-like activity Homo sapiens acetyl-hArg-Pro-Abu-Asp + 7-amino-4-methylcoumarin - ? 449507 3.4.25.1 acetyl-HHSL-7-amido-4-carbamoylcoumarin + H2O - Homo sapiens ? - ? 367788 3.4.25.1 acetyl-norleucine-Arg-norleucine-Arg-7-amido-4-carbamoylcoumarin + H2O - Homo sapiens ? - ? 367786 3.4.25.1 acetyl-norleucine-Arg-norleucine-Arg-7-amido-4-carbamoylcoumarin + H2O - Trypanosoma brucei ? - ? 367786 3.4.25.1 acetyl-norleucine-Leu-Pro-norleucine-Leu-YVAD-7-amido-4-methylcoumarin + H2O - Saccharomyces cerevisiae acetyl-norleucine-Leu-Pro-norleucine-Leu-YVAD + 7-amino-4-methylcoumarin - ? 367789 3.4.25.1 acetyl-norleucine-Leu-Pro-norleucine-Leu-YVAD-7-amido-4-methylcoumarin + H2O - Oryctolagus cuniculus acetyl-norleucine-Leu-Pro-norleucine-Leu-YVAD + 7-amino-4-methylcoumarin - ? 367789 3.4.25.1 acetyl-Phe-Thr(Bzl)-His(3-benzyloxymethyl)-Leu-7-amido-4-methylcoumarin + H2O substrate for chymotrypsin-like activity Homo sapiens acetyl-Phe-Thr(Bzl)-His(3-benzyloxymethyl)-Leu + 7-amino-4-methylcoumarin - ? 449511 3.4.25.1 acetyl-YVAD-7-amido-4-methylcoumarin + H2O - Saccharomyces cerevisiae acetyl-YVAD + 7-amino-4-methylcoumarin - ? 362257 3.4.25.1 acetyl-YVAD-7-amido-4-methylcoumarin + H2O - Oryctolagus cuniculus acetyl-YVAD + 7-amino-4-methylcoumarin - ? 362257 3.4.25.1 acetyl-YWTQ-7-amido-4-carbamoylcoumarin + H2O - Homo sapiens ? - ? 367787 3.4.25.1 acetyl-YWTQ-7-amido-4-carbamoylcoumarin + H2O - Trypanosoma brucei ? - ? 367787 3.4.25.1 Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O - Rattus norvegicus ? - ? 95059 3.4.25.1 Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O - Thermoplasma acidophilum ? - ? 95059 3.4.25.1 Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O - Methanosarcina thermophila ? - ? 95059 3.4.25.1 Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O - Mus musculus Ala-Ala-Phe + 7-amino-4-methylcoumarin - ? 361588 3.4.25.1 Ala-Phe-Lys-7-amido-4-methylcoumarin + H2O - Methanosarcina thermophila ? - ? 95061 3.4.25.1 benzoyl-LRR-4-methyl-7-amido-coumarin + H2O trypsin-like proteasome activity Homo sapiens benzoyl-LRR + 4-methyl-7-amino-coumarin - ? 414345 3.4.25.1 benzoyl-LRR-4-methyl-7-amido-coumarin + H2O trypsin-like proteasome activity with the specific fluorogenic substrate Homo sapiens benzoyl-LRR + 4-methyl-7-amino-coumarin - ? 414345 3.4.25.1 benzoyl-Phe-Val-Arg-7-amido-4-methylcoumarin + H2O - Methanosarcina thermophila ? - ? 95056 3.4.25.1 benzoyl-VGR-4-methyl-7-amido-coumarin + H2O trypsin-like proteasome activity Rattus norvegicus benzoyl-VGR + 4-methyl-7-amino-coumarin - ? 414346 3.4.25.1 benzoyl-VGR-7-amido-4-methylcoumarin + H2O - Rattus norvegicus ? - ? 367757 3.4.25.1 benzyl-Val-Gly-Arg-7-amido-4-methylcoumarin + H2O - Saccharomyces cerevisiae ? - ? 396581 3.4.25.1 benzyloxycarbonyl-Ala-Ala-Leu-4-nitroanilide + H2O - Methanocaldococcus jannaschii ? - ? 367751 3.4.25.1 benzyloxycarbonyl-Ala-Arg-Arg-Arg-7-amido-4-methylcoumarin + H2O - Saccharomyces cerevisiae ? - ? 384276 3.4.25.1 benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O - Trypanosoma brucei ? - ? 95074 3.4.25.1 benzyloxycarbonyl-ARR-7-amido-4-methylcoumarin + H2O - Struthio camelus ? - ? 367771 3.4.25.1 benzyloxycarbonyl-D-Ala-Leu-Arg-4-nitroanilide + H2O - Bos taurus ? - ? 367768 3.4.25.1 benzyloxycarbonyl-dALR-2-naphthylamide + H2O - Bos taurus ? - ? 367796 3.4.25.1 benzyloxycarbonyl-FR-7-amido-4-methylcoumarin + H2O - Struthio camelus ? - ? 367774 3.4.25.1 benzyloxycarbonyl-GAPLG-p-aminobenzoate + H2O - Bos taurus ? - ? 367765 3.4.25.1 benzyloxycarbonyl-GGF-4-aminobenzoate + H2O - Bos taurus ? - ? 453312 3.4.25.1 benzyloxycarbonyl-GGF-p-aminobenzoate + H2O - Bos taurus ? - ? 367767 3.4.25.1 benzyloxycarbonyl-GGL-2-naphthylamide + H2O - Bos taurus ? - ? 367763 3.4.25.1 benzyloxycarbonyl-GGL-4-nitroanilide + H2O chymotrypsin-like activity Candida albicans ? - ? 367752 3.4.25.1 benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin + H2O - Struthio camelus benzyloxycarbonyl-GGL + 7-amino-4-methylcoumarin - ? 362705 3.4.25.1 benzyloxycarbonyl-GGR-2-naphthylamide + H2O trypsin-like activity Candida albicans ? - ? 367753 3.4.25.1 benzyloxycarbonyl-GGR-7-amido-4-methylcoumarin + H2O - Struthio camelus ? - ? 367770 3.4.25.1 benzyloxycarbonyl-GGR-7-amido-4-methylcoumarin + H2O tryspin-like activity Phlebia radiata ? - ? 367770 3.4.25.1 benzyloxycarbonyl-Gly-Gly-Arg-beta-naphthylamide + H2O - Manduca sexta ? - ? 95066 3.4.25.1 benzyloxycarbonyl-Gly-Gly-Leu-4-nitroanilide + H2O - Bos taurus ? - ? 367781 3.4.25.1 benzyloxycarbonyl-Gly-Gly-Leu-7-amido-4-methylcoumarin + H2O - Saccharomyces cerevisiae ? - ? 95075 3.4.25.1 benzyloxycarbonyl-Gly-Gly-Leu-7-amido-4-methylcoumarin + H2O - Trypanosoma brucei ? - ? 95075 3.4.25.1 benzyloxycarbonyl-Gly-Gly-Leu-7-amido-4-methylcoumarin + H2O - Rhodococcus sp. ? - ? 95075 3.4.25.1 benzyloxycarbonyl-Gly-Gly-Leu-7-amido-4-methylcoumarin + H2O - Struthio camelus ? - ? 95075 3.4.25.1 benzyloxycarbonyl-Gly-Gly-Leu-p-nitroanilide + H2O - Manduca sexta ? - ? 95067 3.4.25.1 benzyloxycarbonyl-Gly-Gly-Leu-p-nitroanilide + H2O - Struthio camelus ? - ? 95067 3.4.25.1 benzyloxycarbonyl-Gly-Pro-Ala-Gly-Gly-p-aminobenzoate + aminopeptidase-N + H2O - Manduca sexta ? - ? 95070 3.4.25.1 benzyloxycarbonyl-Gly-Pro-Ala-Leu-Ala-p-aminobenzoate + aminopeptidase-N + H2O - Manduca sexta ? - ? 95069 3.4.25.1 benzyloxycarbonyl-GPAFG-4-aminobenzoate + H2O - Bos taurus ? - ? 453313 3.4.25.1 benzyloxycarbonyl-GPAFG-p-aminobenzoate + H2O - Bos taurus ? - ? 367766 3.4.25.1 benzyloxycarbonyl-GPAGG-4-aminobenzoate + H2O highest activity in soleus and brain Rattus norvegicus ? - ? 367762 3.4.25.1 benzyloxycarbonyl-GPAGG-4-nitroanilide + H2O - Bos taurus ? - ? 367769 3.4.25.1 benzyloxycarbonyl-GPALG-4-aminobenzoate + H2O - Bos taurus ? - ? 367761 3.4.25.1 benzyloxycarbonyl-GPALG-4-aminobenzoate + H2O highest activity in testicle, brain and soleus Rattus norvegicus ? - ? 367761 3.4.25.1 benzyloxycarbonyl-GPALG-p-aminobenzoate + H2O - Bos taurus ? - ? 367764 3.4.25.1 benzyloxycarbonyl-LAF-4-aminobenzoate + H2O - Bos taurus ? - ? 367795 3.4.25.1 benzyloxycarbonyl-Leu-Leu-Glu-2-naphthylamide + H2O - Saccharomyces cerevisiae ? - ? 384301 3.4.25.1 benzyloxycarbonyl-Leu-Leu-Glu-7-amido-4-methylcoumarin + H2O 11% of the activity with succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin Patiria pectinifera ? - ? 367780 3.4.25.1 benzyloxycarbonyl-Leu-Leu-Glu-beta-naphthylamide + H2O - Rattus norvegicus ? - ? 95068 3.4.25.1 benzyloxycarbonyl-Leu-Leu-Glu-beta-naphthylamide + H2O - Saccharomyces cerevisiae ? - ? 95068 3.4.25.1 benzyloxycarbonyl-Leu-Leu-Glu-beta-naphthylamide + H2O - Trypanosoma brucei ? - ? 95068 3.4.25.1 benzyloxycarbonyl-Leu-Leu-Glu-beta-naphthylamide + H2O - Methanosarcina thermophila ? - ? 95068 3.4.25.1 benzyloxycarbonyl-Leu-Leu-Glu-beta-naphthylamide + H2O - Manduca sexta ? - ? 95068 3.4.25.1 benzyloxycarbonyl-Leu-Leu-Leu-7-amido-4-methylcoumarin + H2O 36% of the activity with succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin Patiria pectinifera ? - ? 367779 3.4.25.1 benzyloxycarbonyl-LLE-2-naphthylamide + H2O - Bos taurus ? - ? 367754 3.4.25.1 benzyloxycarbonyl-LLE-2-naphthylamide + H2O - Struthio camelus ? - ? 367754 3.4.25.1 benzyloxycarbonyl-LLE-2-naphthylamide + H2O highest activity in testicle Rattus norvegicus ? - ? 367754 3.4.25.1 benzyloxycarbonyl-LLE-2-naphthylamide + H2O peptidyl-glutamyl-peptide-hydrolyzing activity Candida albicans ? - ? 367754 3.4.25.1 benzyloxycarbonyl-LLE-7-amido-4-methylcoumarin + H2O - Rattus norvegicus ? - ? 367756 3.4.25.1 benzyloxycarbonyl-LLE-beta-naphthylamide + H2O caspase-like activity Phlebia radiata ? - ? 396589 3.4.25.1 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O - Trypanosoma brucei ? - ? 95073 3.4.25.1 benzyloxycarbonyl-RR-7-amido-4-methylcoumarin + H2O - Struthio camelus ? - ? 367772 3.4.25.1 beta-casein + H2O - Bos taurus ? - ? 36618 3.4.25.1 Boc-Leu-Arg-Arg-7-amido-4-methylcoumarin + H2O for trypsin-like activity Mus musculus ? - ? 420409 3.4.25.1 Boc-Leu-Arg-Arg-7-amido-4-methylcoumarin + H2O for trypsin-like activity Mus musculus C57BL/6 ? - ? 420409 3.4.25.1 Boc-Leu-Arg-Arg-7-amido-4-methylcoumarin + H2O trypsin-like activity Homo sapiens Boc-Leu-Arg-Arg + 7-amino-4-methylcoumarin - ? 420410 3.4.25.1 Boc-Leu-Arg-Arg-7-amido-4-methylcoumarin + H2O trypsin-like activity Arabidopsis thaliana Boc-Leu-Arg-Arg + 7-amino-4-methylcoumarin - ? 420410 3.4.25.1 Boc-Leu-Arg-Arg-7-amido-4-methylcoumarin + H2O trypsin-like activity Arabidopsis thaliana Col-0 Boc-Leu-Arg-Arg + 7-amino-4-methylcoumarin - ? 420410 3.4.25.1 Boc-Leu-Leu-Glu-4-methylcoumarin-7-amide + H2O - Homo sapiens Boc-Leu-Leu-Glu + 7-amino-4-methylcoumarin - ? 449696 3.4.25.1 Boc-Leu-Ser-Thr-Arg-7-amido-4-methylcoumarin + H2O - Mus musculus Boc-Leu-Ser-Thr-Arg + 7-amino-4-methylcoumarin - ? 414383 3.4.25.1 Bz-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O - Homo sapiens ? - ? 396640 3.4.25.1 Bz-DL-Arg-7-amido-4-methylcoumarin + H2O - Homo sapiens ? - ? 396641 3.4.25.1 Bz-VGR-7-amido-4-methylcoumarin + H2O - Homo sapiens ? - ? 396643 3.4.25.1 casein + H2O - Homo sapiens ? - ? 15445 3.4.25.1 casein + H2O - Candida albicans ? - ? 15445 3.4.25.1 DBC2 protein + H2O - Homo sapiens ? - ? 396968 3.4.25.1 dihydrofolate reductase + H2O directly hydrolyzed by the 20S complex, without any previous ubiquitination. The degradation is increased under oxidative conditions. The folate metabolism may be impaired by an increased degradation of dihydrofolate reductase, mediated by the 20S proteasome Bos taurus ? - ? 383125 3.4.25.1 dihydrofolate reductase + H2O directly hydrolyzed by the 20S complex, without any previous ubiquitination. The degradation is increased under oxidative conditions Bos taurus ? - ? 383125 3.4.25.1 ERM transcription factor + H2O - Homo sapiens ? - ? 397119 3.4.25.1 erythroid Krueppel-like factor + H2O - Mus musculus ? - ? 397120 3.4.25.1 fructose-1,6-bisphosphatase + H2O - Saccharomyces cerevisiae ? - ? 95082 3.4.25.1 Glu-Gly-Gly-7-amido-4-methylcoumarin + H2O - Thermoplasma acidophilum ? - ? 95065 3.4.25.1 Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O - Methanosarcina thermophila ? - ? 95063 3.4.25.1 Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O - Trypanosoma brucei ? - ? 95063 3.4.25.1 H-VLK-7-amido-4-methylcoumarin + H2O very-low activity Struthio camelus ? - ? 367775 3.4.25.1 hepatitis B virus X protein + H2O - Hepatitis B virus ? - ? 95077 3.4.25.1 IGF-1 + H2O - Homo sapiens ? - ? 397457 3.4.25.1 IkappaBalpha + H2O can be directly degraded by 20S proteasomes. Deletion constructs of IkappaBalpha allow us to the determine that N-terminal (DELTA1-70)and C-terminal regions (DELTA280-327, removing the PEST region) of IkappaBalpha are not required for IkappaBalpha degradation,while a further C-terminal deletion including part of the arm repeats (DELTAC2 245-327) almost completely suppress the degradation by 20S proteasome. Degradation of IkappaBalpha involves specific interactions with a C3 subunit of the proteasome Rattus norvegicus ? - ? 358989 3.4.25.1 Ile-Ile-Trp-7-amido-4-methylcoumarin + H2O - Methanosarcina thermophila ? - ? 95060 3.4.25.1 Leu-Arg-Arg + H2O - Hepatitis B virus ? - ? 95079 3.4.25.1 Leu-Leu-Glu + H2O - Hepatitis B virus ? - ? 95080 3.4.25.1 Leu-Leu-Val-Tyr + H2O - Hepatitis B virus ? - ? 95078 3.4.25.1 Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O - Methanosarcina thermophila ? - ? 95058 3.4.25.1 MATalpha2 repressor + H2O - Saccharomyces cerevisiae ? - ? 95083 3.4.25.1 methyl-casein + H2O - Allium sativum ? - ? 385182 3.4.25.1 additional information overall substrate preference for hydrophobic residues at the P1 to P4 positions in a substrate. This overall stringency is relaxed in the 11 S regulatory (PA26)-20 S proteasome complex, which shows both appreciable activities for cleavage after acidic amino acids and a broadened activity for cleavage after basic amino acids. The 20S proteasome shows appreciable activity for cleavage after P1-Gln that is minimally observed in the human counterpart Trypanosoma brucei ? - ? 89 3.4.25.1 additional information proteasomes are the primary sites for protein degradation in mammalian cells. Each proteasome particle contains two chymotrypsin-like, two trypsin-like, and two caspase-like proteolytic sites. Caspase-like sites cleave after aspartates better than after glutamates Oryctolagus cuniculus ? - ? 89 3.4.25.1 additional information the 20S proteasome of Trypanosoma brucei shows appreciable activity for cleavage after P1-Gln that is minimally observed in the human counterpart Homo sapiens ? - ? 89 3.4.25.1 additional information proteasomes are the primary sites for protein degradation in mammalian cells. Each proteasome particle contains two chymotrypsin-like, two trypsin-like, and two caspase-like proteolytic sites Oryctolagus cuniculus ? - ? 89 3.4.25.1 additional information the chymotrypsin-like and trypsin-like activities, but not the peptidylglutamyl peptide hydrolyzing activity plays a key role in oocyte maturation Patiria pectinifera ? - ? 89 3.4.25.1 additional information the tobacco mosaic virus-induced RNP7 subunit may be involved in programmed cell death Capsicum annuum ? - ? 89 3.4.25.1 additional information maximal chymotrypsin-like activity of the 20S proteasome, which contributes to the cytolytic mechanism of the natural killer cells, is associated with the conformational changes occuring in a cluster of highly conserved proteasome residues from the alpha-subunit that lead to the proteasome open conformation, allowing substrate access into the proteolytic chamber Rattus norvegicus ? - ? 89 3.4.25.1 additional information proteasome status in KG1a and U937 cells, overview Homo sapiens ? - ? 89 3.4.25.1 additional information the proteasome is a cylindrical, multicatalytic proteolytic machine with three peptidase activities, chymotryptic, tryptic and postglutamyl peptide hydrolytic Rattus norvegicus ? - ? 89 3.4.25.1 additional information three major proteolytic activities of the proteasome can be distinguished as trypsin-like, chymotrypsin-like, and peptidyl-glutamyl peptide hydrolase activities, which cleave peptide bonds on the carboxyl side of basic, hydrophobic, and acidic amino acid residues, respectively. The catalytic core of the 20S proteasome is a Thr residue, responsible for the catalytic cleavage of substrates through nucleophilic attack Homo sapiens ? - ? 89 3.4.25.1 additional information proteasome complexes possess three main catalytic activities, which are trypsin-like, chymotrypsin-like, and caspase-like Homo sapiens ? - ? 89 3.4.25.1 additional information substrate docking sites, e.g. S5a, contain ubiquitin-interacting motifs that selectively recruit ubiquinated proteins to the proteasome. Human S5a-ubiquitin-interacting motifs stabilize only a subset of proteasomal substrates. Proliferation of A549 lung cancer cells is inhibited by S5a-ubiquitin-interacting motifs. S5a-ubiquitin-interacting motifs result in an increase in the number of apoptotic G0 cells, presumably because of inhibition of a subset of proteasomal substrate proteins Homo sapiens ? - ? 89 3.4.25.1 additional information the proteasome cannot efficiently degrade unassembled R239C GFAP, and the interaction of R239C GFAP with proteasomes actually inhibits proteasomal protease activity. Soluble wild-type GFAP, but not R239C GFAP, is partially degraded by the 20 S proteasome Homo sapiens ? - ? 89 3.4.25.1 additional information the purified proteasome, comprising 102 distinct proteins, shows high chymotrypsin-like activity, mass spectrometry and protein identifications, overview Trichoderma reesei ? - ? 89 3.4.25.1 additional information mass spectrometric analysis of proteasome interactions. Several proteasome-interacting proteins unique to synaptic 26S proteasomes, i.e. 14-3-3gamma, TAX1BP1, drebrin, SNAP-25, may modulate proteolysis in a synapse-specific manner. Three E3s, i.e. KCMF1, HUWE1, and UBE3A, and five DUBs, i.e. USP5, USP7, USP13, USP14, and UCH37, in association with synaptic proteasomes, which may help proteasomes function more efficiently, help determine specificity for certain types of conjugates, or insure the rapid elimination of ubiquitin chains released from the substrate Rattus norvegicus ? - ? 89 3.4.25.1 additional information several other proteins associate with the Arabidopsis thaliana proteasome, including the PBAC2 assembly chaperonin, the associated DSS1/Sem1/RPN15 protein, the deubiquitylating enzyme UBP16, and the alternative activator PA200, genetic analysis of PA200 in Arabidopsis, overview Arabidopsis thaliana ? - ? 89 3.4.25.1 additional information enzyme has a preference for aromatic residues at P1 such as Phe, Tyr, Trp, and His. Aliphatic side chains (propyl, butyl) are well tolerated in the P2 position. The S3 pocket accommodates bulky and hydrophobic residues Plasmodium falciparum ? - ? 89 3.4.25.1 additional information mass spectrometric analysis of proteasome interactions. Several proteasome-interacting proteins unique to synaptic 26S proteasomes, i.e. 14-3-3gamma, TAX1BP1, drebrin, SNAP-25, may modulate proteolysis in a synapse-specific manner. Three E3s, i.e. KCMF1, HUWE1, and UBE3A, and five DUBs, i.e. USP5, USP7, USP13, USP14, and UCH37, in association with synaptic proteasomes, which may help proteasomes function more efficiently, help determine specificity for certain types of conjugates, or insure the rapid elimination of ubiquitin chains released from the substrate Rattus norvegicus Sprague-Dawley ? - ? 89 3.4.25.1 additional information enzyme has a preference for aromatic residues at P1 such as Phe, Tyr, Trp, and His. Aliphatic side chains (propyl, butyl) are well tolerated in the P2 position. The S3 pocket accommodates bulky and hydrophobic residues Plasmodium falciparum W2 ? - ? 89 3.4.25.1 additional information several other proteins associate with the Arabidopsis thaliana proteasome, including the PBAC2 assembly chaperonin, the associated DSS1/Sem1/RPN15 protein, the deubiquitylating enzyme UBP16, and the alternative activator PA200, genetic analysis of PA200 in Arabidopsis, overview Arabidopsis thaliana Col-0 ? - ? 89 3.4.25.1 morpholinoacetyl-homophenylalanyl-methylseryl-thienylalanyl-7-amido-4-carbamoylmethylcoumarin + H2O most optimal combination of substrate residues identified Plasmodium falciparum morpholinoacetyl-homophenylalanine-methylserine-thienylalanine + 7-amino-4-carbamoylmethylcoumarin - ? 450300 3.4.25.1 morpholinoacetyl-homophenylalanyl-methylseryl-thienylalanyl-7-amido-4-carbamoylmethylcoumarin + H2O most optimal combination of substrate residues identified Plasmodium falciparum W2 morpholinoacetyl-homophenylalanine-methylserine-thienylalanine + 7-amino-4-carbamoylmethylcoumarin - ? 450300 3.4.25.1 N-Cbz-Leu-Leu-Glu-beta-naphthylamide + H2O - Rattus norvegicus ? - ? 397884 3.4.25.1 N-methoxysuccinyl-Glu-Val-Lys-Met-p-nitroanilide + H2O - Homo sapiens ? - ? 95081 3.4.25.1 N-succinyl-LLVY-7-amido-4-methylcoumarin + H2O - Rattus norvegicus ? - ? 367755 3.4.25.1 N-succinyl-LLVY-7-amido-4-methylcoumarin + H2O - Struthio camelus ? - ? 367755 3.4.25.1 N-succinyl-LLVY-7-amido-4-methylcoumarin + H2O highest activity in testicle, liver and spleen Rattus norvegicus ? - ? 367755 3.4.25.1 N-succinyl-LLVY-7-amido-4-methylcoumarin + H2O chymotrypsin-like proteasome activity Homo sapiens N-succinyl-LLVY + 7-amino-4-methylcoumarin - ? 412572 3.4.25.1 N-succinyl-LLVY-7-amido-4-methylcoumarin + H2O chymotrypsin-like proteasome activity Rattus norvegicus N-succinyl-LLVY + 7-amino-4-methylcoumarin - ? 412572 3.4.25.1 N-succinyl-LLVY-7-amido-4-methylcoumarin + H2O chymotrypsin-like proteasome activity Oryctolagus cuniculus N-succinyl-LLVY + 7-amino-4-methylcoumarin - ? 412572 3.4.25.1 N-succinyl-LLVY-7-amido-4-methylcoumarin + H2O chymotrypsin-like proteasome activity with the specific fluorogenic substrate Homo sapiens N-succinyl-LLVY + 7-amino-4-methylcoumarin - ? 412572 3.4.25.1 N-succinyl-LLVY-aminoluciferin + H2O - Homo sapiens ? - ? 434026 3.4.25.1 N-t-Boc-Leu-Ser-Thr-Arg-7-amido-4-methylcoumarin + H2O - Rattus norvegicus ? - ? 397926 3.4.25.1 N-tert-butyloxycarbonyl-LSTR-7-amido-4-methylcoumarin + H2O highest activity in kidney Rattus norvegicus ? - ? 367760 3.4.25.1 ovalbumin + H2O - Homo sapiens ? - ? 37064 3.4.25.1 oxidized insulin B chain + H2O - Homo sapiens ? - ? 37092 3.4.25.1 p27(KIP1) + H2O - Homo sapiens ? - ? 398052 3.4.25.1 Pro-Phe-Arg-7-amido-4-methylcoumarin + H2O - Methanosarcina thermophila ? - ? 95062 3.4.25.1 PS1/gamma-secretase complex component + H2O gamma-secretase components are PS1, nicastrin, Pen-2, and Aph-1. Degradation of the complex components involves the proteasome, but regulation of their activity involves the PI3K/Akt pathway, overview. PS1/gamma-secretase is involved in the activation of phosphatidylinositol-3 kinase/Akt pathway, and is responsible for the intramembranous cleavage of various type-I membrane proteins. PS1/gamma-secretase is also deeply involved in the production of amyloid beta protein Mus musculus ? - ? 413558 3.4.25.1 PS1/gamma-secretase complex component + H2O gamma-secretase components are PS1, nicastrin, Pen-2, and Aph-1 Mus musculus ? - ? 413558 3.4.25.1 S-RNase + H2O S-RNase is ubiquitinated and degraded by the 26S proteasome Petunia integrifolia subsp. inflata ? - ? 398296 3.4.25.1 Suc-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O - Mus musculus 7-amino-4-methylcoumarin + Suc-Leu-Leu-Val-Tyr - ? 415336 3.4.25.1 Suc-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O for chymotrypsin-like activity Mus musculus ? - ? 421534 3.4.25.1 Suc-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O for chymotrypsin-like activity Mus musculus C57BL/6 ? - ? 421534 3.4.25.1 succinyl-AAF-7-amido-4-methylcoumarin + H2O - Struthio camelus ? - ? 367778 3.4.25.1 succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O - Rattus norvegicus ? - ? 95054 3.4.25.1 succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O - Thermoplasma acidophilum ? - ? 95054 3.4.25.1 succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O - Rhodococcus sp. ? - ? 95054 3.4.25.1 succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O - Methanosarcina thermophila ? - ? 95054 3.4.25.1 succinyl-Ile-Ile-Trp-7-amido-4-methylcoumarin + H2O - Methanosarcina thermophila ? - ? 95055 3.4.25.1 succinyl-Leu-Leu-Val-Tyr-4-methylcoumarin-7-amide + H2O - Homo sapiens succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin - ? 450695 3.4.25.1 succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O - Drosophila melanogaster ? - ? 95053 3.4.25.1 succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O - Mus musculus ? - ? 95053 3.4.25.1 succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O - Homo sapiens ? - ? 95053 3.4.25.1 succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O - Rattus norvegicus ? - ? 95053 3.4.25.1 succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O - Saccharomyces cerevisiae ? - ? 95053 3.4.25.1 succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O - Bos taurus ? - ? 95053 3.4.25.1 succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O - Oryctolagus cuniculus ? - ? 95053 3.4.25.1 succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O - Spinacia oleracea ? - ? 95053 3.4.25.1 succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O - Arabidopsis thaliana ? - ? 95053 3.4.25.1 succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O - Thermoplasma acidophilum ? - ? 95053 3.4.25.1 succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O - Rhodococcus sp. ? - ? 95053 3.4.25.1 succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O - Patiria pectinifera ? - ? 95053 3.4.25.1 succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O - Methanosarcina thermophila ? - ? 95053 3.4.25.1 succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O - Psophocarpus tetragonolobus ? - ? 95053 3.4.25.1 succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O - Trypanosoma brucei ? - ? 95053 3.4.25.1 succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O - Ceratitis capitata ? - ? 95053 3.4.25.1 succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O chymotrypsin-like activity Phlebia radiata ? - ? 95053 3.4.25.1 succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O substrate for chymotrypsin-like activity of the 20S proteasome Solanum lycopersicum ? - ? 95053 3.4.25.1 succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O - Homo sapiens succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin - ? 359008 3.4.25.1 succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O - Rattus norvegicus succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin - ? 359008 3.4.25.1 succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O - Arabidopsis thaliana succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin - ? 359008 3.4.25.1 succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O - Dicentrarchus labrax succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin - ? 359008 3.4.25.1 succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O - Chionodraco hamatus succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin - ? 359008 3.4.25.1 succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O - Rattus norvegicus Sprague-Dawley succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin - ? 359008 3.4.25.1 succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O - Arabidopsis thaliana Col-0 succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin - ? 359008 3.4.25.1 succinyl-Leu-Met-7-amido-4-methylcoumarin + H2O - Rattus norvegicus ? - ? 95072 3.4.25.1 succinyl-LLVY-7-amido-4-methylcoumarin + H2O - Struthio camelus succinyl-LLVY + 7-amino-4-methylcoumarin - ? 362706 3.4.25.1 succinyl-LLVY-aminoluciferin + H2O chymotrypsin-like proteasome activity Mus musculus succinyl-LLVY + aminoluciferin - ? 415342 3.4.25.1 succinyl-LLVY-aminoluciferin + H2O chymotrypsin-like proteasome activity Homo sapiens succinyl-LLVY + aminoluciferin - ? 415342 3.4.25.1 succinyl-LLVY-aminoluciferin + H2O chymotrypsin-like proteasome activity Rattus norvegicus succinyl-LLVY + aminoluciferin - ? 415342 3.4.25.1 t-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin + H2O - Rattus norvegicus ? - ? 95071 3.4.25.1 t-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin + H2O - Psophocarpus tetragonolobus ? - ? 95071 3.4.25.1 t-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O - Rattus norvegicus ? - ? 95057 3.4.25.1 t-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O - Methanosarcina thermophila ? - ? 95057 3.4.25.1 tert-butyloxycarbonyl-Leu-Arg-Arg-4-methylcoumarin-7-amide - Homo sapiens ? - ? 450746 3.4.25.1 tert-butyloxycarbonyl-LRR-7-amido-4-methylcoumarin + H2O - Struthio camelus ? - ? 367773 3.4.25.1 tert-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O 65% of the activity with succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin Patiria pectinifera ? - ? 365542 3.4.25.1 Tyr-Leu-Leu-Leu-vinylsulfone + H2O - Trypanosoma brucei ? - ? 367783 3.4.25.1 Tyr-Val-Ala-Asp-7-amido-4-methylcoumarin + H2O - Methanosarcina thermophila ? - ? 95064 3.4.25.1 ubiquitinylated proteins individual substrates unknown Drosophila melanogaster ? - ? 369796 3.4.25.1 ubiquitinylated proteins individual substrates unknown Homo sapiens ? - ? 369796 3.4.25.1 ubiquitinylated proteins individual substrates unknown Saccharomyces cerevisiae ? - ? 369796 3.4.25.1 ubiquitinylated proteins individual substrates unknown Oryctolagus cuniculus ? - ? 369796 3.4.25.1 ubiquitinylated proteins individual substrates unknown Thermoplasma acidophilum ? - ? 369796 3.4.25.1 ubiquitinylated proteins individual substrates unknown Xenopus laevis ? - ? 369796 3.4.25.1 ubiquitinylated proteins individual substrates unknown Schizosaccharomyces pombe ? - ? 369796 3.4.25.1 unstable green fluorescence protein + H2O artificial substrate for the proteasome. The model proteasomal substrate is stabilized by the carboxyl-terminal half of S5a, S5aC Homo sapiens ? - ? 415422 3.4.25.1 Z-Ala-Arg-Arg-7-amido-4-methylcoumarin + H2O - Mus musculus ? - ? 398583 3.4.25.1 Z-Leu-Leu-Glu-7-amido-4-methylcoumarin + H2O - Mus musculus Z-Leu-Leu-Glu + 7-amino-4-methylcoumarin - ? 398584 3.4.25.1 Z-Leu-Leu-Glu-7-amido-4-methylcoumarin + H2O for caspase-like activity Mus musculus Z-Leu-Leu-Glu + 7-amino-4-methylcoumarin - ? 398584 3.4.25.1 Z-Leu-Leu-Glu-7-amido-4-methylcoumarin + H2O for caspase-like activity Mus musculus C57BL/6 Z-Leu-Leu-Glu + 7-amino-4-methylcoumarin - ? 398584 3.4.25.1 Z-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O for calpain-like activity Mus musculus Z-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin - ? 421708 3.4.25.1 Z-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O for calpain-like activity Mus musculus C57BL/6 Z-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin - ? 421708 3.4.25.1 Z-LLE-2-naphthylamide + H2O post-acidic proteasome activity Homo sapiens Z-LLE + 2-naphthylamine - ? 415464 3.4.25.1 Z-LLE-4-methyl-7-amido-coumarin + H2O peptidylglutamyl peptide hydrolase proteasome activity Homo sapiens Z-LLE + 4-methyl-7-amino-coumarin - ? 415465 3.4.25.1 Z-LLE-4-methyl-7-amido-coumarin + H2O post-acidic proteasome activity Homo sapiens Z-LLE + 4-methyl-7-amino-coumarin - ? 415465 3.4.25.1 Z-LLE-4-methyl-7-amido-coumarin + H2O post-acidic proteasome activity Rattus norvegicus Z-LLE + 4-methyl-7-amino-coumarin - ? 415465 3.4.25.1 Z-LLE-4-methyl-7-amido-coumarin + H2O post-acidic proteasome activity with the specific fluorogenic substrate Homo sapiens Z-LLE + 4-methyl-7-amino-coumarin - ? 415465 3.4.25.1 Z-LLE-7-amido-4-methylcoumarin + H2O - Homo sapiens ? - ? 398585 3.4.25.1 Z-LRR-aminoluciferin + H2O - Homo sapiens ? - ? 434479 3.4.25.1 Z-nLPnLD-aminoluciferin + H2O - Homo sapiens ? - ? 434480