3.4.24.B17 3-deoxy-D-manno-octulosonate transferase + H2O 3-deoxy-D-manno-octulosonate transferase carries out the attachment of two KDO residues to the lipid A precursor (lipid IVA) to form the minimal essential structure of the lipopolysaccharide (KDO2-lipid A). Thus, FtsH regulates the concentration of the lipid moiety of LPS (lipid A) as well as the sugar moiety (KDO-based core oligosaccharides), ensuring a balanced synthesis of lipopolysaccharide Escherichia coli ? - ? 398659 3.4.24.B17 alpha-casein + H2O - Escherichia coli ? - ? 366244 3.4.24.B17 alpha-casein + H2O - Escherichia coli AR796 ? - ? 366244 3.4.24.B17 apo-flavodoxin + H2O degradation of apo-flavodoxin from Escherichia coli, no degradation of holo-flavodoxin containing non-covalently bound flavin mononucleotide. A mutant flavodoxin carrying a substitution of Tyr94 to Asp (FldYD) with a lower affinity for FMN is efficiently degraded. FtsH is able to initiate degradation of the FldYD moiety even when it is sandwiched by glutathione S-transferase, green fluorescent protein, or both green fluorescent protein and glutathione S-transferase Escherichia coli ? - ? 384202 3.4.24.B17 ATP + H2O - Escherichia coli ? - ? 64622 3.4.24.B17 ATP + H2O - Escherichia coli AR796 ? - ? 64622 3.4.24.B17 bacteriophage lambdaCII repressor protein + H2O - Mycobacterium tuberculosis ? - ? 384250 3.4.24.B17 barnase tagged with SsrA tail + H2O tail specific degradation Escherichia coli ? - ? 367609 3.4.24.B17 beta-casein + H2O - Bacillus subtilis ? - ? 36618 3.4.24.B17 beta-casein + H2O - Bacillus subtilis 168 ? - ? 36618 3.4.24.B17 beta-casein + H2O - Escherichia coli small peptides of 13-20 amino acid residues product analysis ? 367598 3.4.24.B17 biotin carboxylase/biotin carboxyl carrier protein + H2O - Corynebacterium glutamicum ? - ? 391510 3.4.24.B17 BODIPY-casein + H2O - Escherichia coli ? - ? 381587 3.4.24.B17 casein + H2O - Escherichia coli ? - ? 15445 3.4.24.B17 casein + H2O resorufin-labeled substrate Escherichia coli ? - ? 15445 3.4.24.B17 casein + H2O caseinolytic activity is not stimulated by ATP Thermotoga maritima ? - ? 15445 3.4.24.B17 casein + H2O - Escherichia coli W3110 / ATCC 27325 ? - ? 15445 3.4.24.B17 casein + H2O - Escherichia coli peptides - ? 367602 3.4.24.B17 chimeric protein PhoA-TM8-C30 + H2O recombinant substrate protein consists of one transmembrane segment of protein SecY, i.e. TM8, plus the following 30 residues of a cytoplasmic part, and the C-terminal end of alkaline phosphatase PhoA Escherichia coli ? - ? 367600 3.4.24.B17 CII protein of phage lambda + H2O - Escherichia coli ? - ? 367593 3.4.24.B17 colicin D + H2O - Escherichia coli ? - ? 419389 3.4.24.B17 colicin D + H2O - Escherichia coli C600 ? - ? 419389 3.4.24.B17 colicin E3 + H2O - Escherichia coli ? - ? 419390 3.4.24.B17 colicin E3 + H2O - Escherichia coli C600 ? - ? 419390 3.4.24.B17 conserved hypothetical protein NCgl1985 + H2O - Corynebacterium glutamicum ? - ? 391686 3.4.24.B17 CTP + H2O - Escherichia coli ? - ? 64642 3.4.24.B17 dihydrofolate reductase tagged with SsrA tail + H2O tail specific degradation Escherichia coli ? - ? 367610 3.4.24.B17 doxorubicin resistance protein B + H2O - Escherichia coli ? - ? 432779 3.4.24.B17 doxorubicin resistance protein B + H2O - Escherichia coli AR796 ? - ? 432779 3.4.24.B17 F0a protein + H2O - Escherichia coli ? - ? 432795 3.4.24.B17 F0a protein + H2O - Escherichia coli W3110 / ATCC 27325 ? - ? 432795 3.4.24.B17 F0a subunit of the ATP synthetase + H2O - Escherichia coli ? - ? 391959 3.4.24.B17 FeoB protein + H2O - Salmonella enterica ? - ? 432797 3.4.24.B17 FGH-(NO2)FFAF-methyl ester + H2O exclusively cleaved at the (NO2)Phe-Phe bond Escherichia coli FGH-(NO2)F + Phe-Ala-Phe-methyl ester - ? 367606 3.4.24.B17 flavodoxin + H2O degrades flavin mononucleotide-free flavodoxin but not holo-flavodoxin Escherichia coli ? - ? 391983 3.4.24.B17 flavodoxin + H2O FtsH degrades the apo form of Anabaena flavodoxin, but it is unable to hydrolyze the holo form Anabaena sp. ? - ? 391983 3.4.24.B17 flavodoxin + H2O degrades flavin mononucleotide-free flavodoxin but not holo-flavodoxin Escherichia coli AR5771 ? - ? 391983 3.4.24.B17 FtsZ protein + H2O - Escherichia coli ? - ? 391998 3.4.24.B17 fusion protein SecY-(P5)-PhoA + H2O recombinant substrate protein consists of one protein SecY without its C-terminus, and the alkaline phosphatase PhoA Escherichia coli ? - ? 367601 3.4.24.B17 glutamate binding protein + H2O - Corynebacterium glutamicum ? - ? 392096 3.4.24.B17 glyceraldehyde-3-phosphate dehydrogenase + H2O - Corynebacterium glutamicum ? - ? 95040 3.4.24.B17 GTP + H2O - Escherichia coli ? - ? 64639 3.4.24.B17 heat shock sigma factor RpoH (sigma32) + H2O in addition to the turnover element in region 2.1, a second region important for proteolysis of RpoH by FtsH lies in region C of the sigma factor Escherichia coli ? - ? 401118 3.4.24.B17 heat shock transcription factor sigma32 protein + H2O - Mycobacterium tuberculosis ? - ? 384848 3.4.24.B17 homocysteine methyltransferase + H2O - Corynebacterium glutamicum ? - ? 392223 3.4.24.B17 isocitrate lyase + H2O - Corynebacterium glutamicum ? - ? 392289 3.4.24.B17 lambda protein CII + H2O cytoplasmic regulatory protein Escherichia coli ? - ? 367599 3.4.24.B17 lambda Xis + H2O protein substrate is required for site-specific excision of phage lambda from the bacterial chromosome Escherichia coli ? - ? 367612 3.4.24.B17 largely unfolded alpha-lactalbumin + H2O no activity with the native protein, cleavage of small peptides from the C-terminal side of hydrophobic residues, no large intermediates Thermus thermophilus peptides between 10 and 30 kDa, no large intermediates ? 367615 3.4.24.B17 LpxC + H2O - Escherichia coli ? - ? 383234 3.4.24.B17 LpxC + H2O ATP-dependent. Essentiality of FtsH lies in its function to keep the proper LPS/phospholipid ratio by degrading LpxC Escherichia coli ? - ? 383234 3.4.24.B17 LpxC + H2O ATP-dependent Escherichia coli ? - ? 383234 3.4.24.B17 LpxC protein + H2O efficient substrate Escherichia coli ? - ? 432838 3.4.24.B17 LpxC protein + H2O efficient substrate Escherichia coli W3110 / ATCC 27325 ? - ? 432838 3.4.24.B17 malate synthase + H2O - Corynebacterium glutamicum ? - ? 392524 3.4.24.B17 MgtC mutant W226A substrate is a virulence protein located at the inner mitochondrial membrane. A single substitution W226A promotes the FtsH-mediated proteolysis. The Trp residue is located at the very C-terminus of the cytoplasmic domain of the MgtC protein Salmonella enterica ? - ? 450286 3.4.24.B17 MgtC mutant W226A substrate is a virulence protein located at the inner mitochondrial membrane. A single substitution W226A promotes the FtsH-mediated proteolysis. The Trp residue is located at the very C-terminus of the cytoplasmic domain of the MgtC protein Salmonella enterica 14028s ? - ? 450286 3.4.24.B17 additional information no activity with bovine serum albumin Escherichia coli ? - ? 89 3.4.24.B17 additional information construction of an in vitro reaction system in which the enzyme is membrane embedded and not solubilized by detergent, two inverted membrane vesicles or proteoliposomes, one bearing the enzyme, the other bearing the substrate protein, are fused Escherichia coli ? - ? 89 3.4.24.B17 additional information enzyme shows overlapping substrate specificity with the proteases C1pXP and C1pAP, the enzymes can compensate for each other in degrading the c1-SsrA tagged substrate protein Escherichia coli ? - ? 89 3.4.24.B17 additional information large complexes exhibit either ATPase and protease activity, while smaller ones do not Escherichia coli ? - ? 89 3.4.24.B17 additional information no activity with GFP-tagged proteins Escherichia coli ? - ? 89 3.4.24.B17 additional information no activity with native proteins as isopropylmalate dehydrogenase, glucose-6-phosphate dehydrogenase from Bacillus stearothermophilus, and bovine pancreas RNaseA Thermus thermophilus ? - ? 89 3.4.24.B17 additional information the enzyme acts in cooperation with the homologous proteins HflK and HflC, the 3 proteins assemble at the periplasmic site of the plasma membrane, resulting in prohibitin-like modulation of the enzymes substrate specificity and activity Escherichia coli ? - ? 89 3.4.24.B17 additional information dislocation of membrane proteins mediated by the enzyme, periplasmic segments can also be degraded by the enzyme Escherichia coli ? - ? 89 3.4.24.B17 additional information no degradation of protein FtsZ in vivo Escherichia coli ? - ? 89 3.4.24.B17 additional information FtsH functions as a membrane chaperone and protease. FtsH and YidC have a linked role in the quality control of inner membrane proteins Escherichia coli ? - ? 89 3.4.24.B17 additional information protein LpxC from A aeolicus is not degraded by FtsH from Escherichia coli Escherichia coli ? - ? 89 3.4.24.B17 additional information protein LpxC from A aeolicus is not degraded by FtsH from Escherichia coli Escherichia coli W3110 / ATCC 27325 ? - ? 89 3.4.24.B17 additional information no activity with native proteins as isopropylmalate dehydrogenase, glucose-6-phosphate dehydrogenase from Bacillus stearothermophilus, and bovine pancreas RNaseA Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ? - ? 89 3.4.24.B17 N-succinyl-LLVY-7-amido-4-methylcoumarin + H2O - Escherichia coli ? - ? 367755 3.4.24.B17 phage lambda CII protein + H2O recombinant protein substrate with -terminal or C-terminal His-tag, respectively, or no His-tag Escherichia coli small peptides of 13-20 amino acid residues product analysis ? 367597 3.4.24.B17 phage lambda CII protein + H2O enzyme participates in the phage lambda lysis-lysogeny decision by degrading the CII transcriptional activator and by its response to inhibition by the lambda CIII gene product Escherichia coli small peptides - ? 370689 3.4.24.B17 phage shock protein C + H2O phage shock protein C is the only core component of the phage shock protein system affected by FtsH. Phage shock protein B prevents FtsH-dependent degradation of phage shock protein C Yersinia enterocolitica ? - ? 421197 3.4.24.B17 phage shock protein C + H2O phage shock protein C is the only core component of the phage shock protein system affected by FtsH. Phage shock protein B prevents FtsH-dependent degradation of phage shock protein C Escherichia coli ? - ? 421197 3.4.24.B17 phage shock protein C + H2O phage shock protein C is the only core component of the phage shock protein system affected by FtsH. Phage shock protein B prevents FtsH-dependent degradation of phage shock protein C Yersinia enterocolitica AJD3 ? - ? 421197 3.4.24.B17 PhoA protein + H2O - Escherichia coli ? - ? 367595 3.4.24.B17 Protein + H2O FtsH degrades misassembled membrane proteins and a subset of cytoplasmic regulatory proteins Escherichia coli ? - ? 15410 3.4.24.B17 Protein + H2O the enzyme can unfold proteins with lower Tms such as glutathione S-transferase (Tm: 52°C) Escherichia coli ? - ? 15410 3.4.24.B17 protein + H2O - Bacillus subtilis peptides - ? 365037 3.4.24.B17 protein + H2O - Escherichia coli peptides - ? 365037 3.4.24.B17 protein + H2O - Thermus thermophilus peptides - ? 365037 3.4.24.B17 protein + H2O ATP hydrolysis cause conformational changes, regulate the accessibility of the proteolytic sites and trigger unfolding of substrate polypeptides, C-terminally located second region of homology, i.e. SRH region, is conserved throughout the AAA proteases and plays an intermolecular catalytic role Escherichia coli peptides - ? 365037 3.4.24.B17 protein + H2O cleavage of small peptides from the C-terminal side of hydrophobic residues, no large intermediates, overview Thermus thermophilus peptides between 10 and 30 kDa, no large intermediates ? 365037 3.4.24.B17 protein + H2O degradation of membrane and cytoplasmic proteins Escherichia coli peptides - ? 365037 3.4.24.B17 protein + H2O degradation probably involves dislocation of the substrate membrane protein to the cytosol Escherichia coli peptides - ? 365037 3.4.24.B17 protein + H2O enzyme recognizes nonpolar tails, overview, degrades integral and cytoplasmic proteins Escherichia coli peptides - ? 365037 3.4.24.B17 protein + H2O enzyme shows limited substrate specificity which is not primarily determined by the structure of the protein Escherichia coli peptides - ? 365037 3.4.24.B17 protein + H2O preference for positively charged and hydrophobic amino acid residues, degradation of uncomplexed integral membrane proteins and short-life cytoplasmic proteins Escherichia coli peptides - ? 365037 3.4.24.B17 protein + H2O substrate binding by the cytoplasmic domain Escherichia coli peptides - ? 365037 3.4.24.B17 protein + H2O substrate specificity, soluble and integral membrane proteins, initiation of degradation by recognition and binding of more than 20 amino acids of the C-terminal cytosolic tail of the substrate, subsequently the substrate can be dislocated into the cytosol or degraded simultaneously bidirectionally Escherichia coli peptides - ? 365037 3.4.24.B17 protein + H2O degradation of regulatory proteins to control gene activity and metabolism Escherichia coli peptides - ? 365037 3.4.24.B17 protein + H2O degrades misassembled membrane protein complexes and plays a vital role in membrane quality control, degrades cytoplasmic regulatory proteins Escherichia coli peptides - ? 365037 3.4.24.B17 protein + H2O enzyme affects several processes including cell division, the synthesis of phospholipids and lipopolysaccharides, the anchoring of integral membrane proteins, mRNA stability, and colchicin tolerance, degradation of membrane proteins, essentially required as a membrane-integrated quality control Escherichia coli peptides - ? 365037 3.4.24.B17 protein + H2O housekeeping function, because the enzyme lacks a robust unfoldase activity, it is able to use the substrate protein folding state as a criterion for degradation Escherichia coli peptides - ? 365037 3.4.24.B17 protein + H2O involved in membrane protein assembly as well as degradation of unstable proteins Escherichia coli peptides - ? 365037 3.4.24.B17 protein + H2O involved in the degradation of regulatory proteins and uncomplexed subunits of membrane protein complexes Escherichia coli peptides - ? 365037 3.4.24.B17 protein + H2O tail specific pathway for removing abnormal cytoplasmic proteins via the enzyme, disposition by degradation of polypeptides synthesized from truncated mRNA molecules and are C-terminally tagged with an 11-amino-acid nonpolar destabilizing tail via a mechanism involving the 10Sa stable RNA Escherichia coli peptides - ? 365037 3.4.24.B17 protein + H2O FtsH degrades a set of short-lived proteins, enabling cellular regulation at the level of protein stability. FtsH also degrades some misassembles membrane proteins, contributing to their quality maintenance. One biological role of FtsH might be to affect the development and life cycle of infecting or episomal genetic systems, by degrading their key regulatory molecules. The enzyme has a special ability to dislocate membrane protein substrates out of the membrane for which its own membrane-embedded nature is essential Escherichia coli peptides - ? 365037 3.4.24.B17 protein + H2O - Thermus thermophilus HB8 / ATCC 27634 / DSM 579 peptides - ? 365037 3.4.24.B17 protein + H2O cleavage of small peptides from the C-terminal side of hydrophobic residues, no large intermediates, overview Thermus thermophilus HB8 / ATCC 27634 / DSM 579 peptides between 10 and 30 kDa, no large intermediates ? 365037 3.4.24.B17 protein D1 + H2O - Thermus thermophilus ? - ? 383339 3.4.24.B17 protein D1 + H2O - Helicobacter pylori ? - ? 383339 3.4.24.B17 protein D1 + H2O - Aquifex aeolicus ? - ? 383339 3.4.24.B17 protein D1 + H2O - Thermotoga maritima ? - ? 383339 3.4.24.B17 protein D2 + H2O - Thermus thermophilus ? - ? 383340 3.4.24.B17 protein D2 + H2O - Helicobacter pylori ? - ? 383340 3.4.24.B17 protein D2 + H2O - Aquifex aeolicus ? - ? 383340 3.4.24.B17 protein D2 + H2O - Thermotoga maritima ? - ? 383340 3.4.24.B17 protein F0 subunit a + H2O - Escherichia coli ? - ? 367586 3.4.24.B17 protein F0 subunit a + H2O subunit a of the F0 part of the H+-ATPase, unstable in the absence of other F0 subunits leading to degradation by the enzyme Escherichia coli ? - ? 367586 3.4.24.B17 protein F0 subunit a + H2O degradation of membrane protein, essentially required as a membrane-integrated quality control Escherichia coli ? - ? 367586 3.4.24.B17 protein F0a + H2O - Thermus thermophilus ? - ? 421233 3.4.24.B17 protein F0a + H2O - Helicobacter pylori ? - ? 421233 3.4.24.B17 protein F0a + H2O - Aquifex aeolicus ? - ? 421233 3.4.24.B17 protein F0a + H2O - Thermotoga maritima ? - ? 421233 3.4.24.B17 protein FtsZ + H2O - Escherichia coli ? - ? 393364 3.4.24.B17 protein FtsZ + H2O - Escherichia coli TYE024 ? - ? 393364 3.4.24.B17 protein GgpS + H2O - Thermus thermophilus ? - ? 421234 3.4.24.B17 protein GgpS + H2O - Helicobacter pylori ? - ? 421234 3.4.24.B17 protein GgpS + H2O - Aquifex aeolicus ? - ? 421234 3.4.24.B17 protein GgpS + H2O - Thermotoga maritima ? - ? 421234 3.4.24.B17 protein GlnK + H2O - Thermus thermophilus ? - ? 421235 3.4.24.B17 protein GlnK + H2O - Helicobacter pylori ? - ? 421235 3.4.24.B17 protein GlnK + H2O - Aquifex aeolicus ? - ? 421235 3.4.24.B17 protein GlnK + H2O - Thermotoga maritima ? - ? 421235 3.4.24.B17 protein GlpG + H2O helical bundle membrane protein, model membrane substrate Escherichia coli ? - ? 450523 3.4.24.B17 protein KdtA + H2O - Thermus thermophilus ? - ? 421236 3.4.24.B17 protein KdtA + H2O - Helicobacter pylori ? - ? 421236 3.4.24.B17 protein KdtA + H2O - Aquifex aeolicus ? - ? 421236 3.4.24.B17 protein KdtA + H2O - Thermotoga maritima ? - ? 421236 3.4.24.B17 protein lambdaCII + H2O - Escherichia coli ? - ? 367588 3.4.24.B17 protein lambdaCII + H2O - Corynebacterium glutamicum ? - ? 367588 3.4.24.B17 protein lambdaCII + H2O - Helicobacter pylori ? - ? 367588 3.4.24.B17 protein lambdaCII + H2O regulatory protein Escherichia coli ? - ? 367588 3.4.24.B17 protein lambdaCII + H2O degradation has regulatory function Escherichia coli ? - ? 367588 3.4.24.B17 protein lambdaCIII + H2O - Escherichia coli ? - ? 367589 3.4.24.B17 protein lambdaCIII + H2O regulatory protein Escherichia coli ? - ? 367589 3.4.24.B17 protein lambdaCIII + H2O degradation has regulatory function Escherichia coli ? - ? 367589 3.4.24.B17 protein lambdaXis + H2O - Escherichia coli ? - ? 367590 3.4.24.B17 protein lambdaXis + H2O degradation has regulatory function Escherichia coli ? - ? 367590 3.4.24.B17 protein LpxC + H2O - Thermus thermophilus ? - ? 367591 3.4.24.B17 protein LpxC + H2O - Escherichia coli ? - ? 367591 3.4.24.B17 protein LpxC + H2O - Helicobacter pylori ? - ? 367591 3.4.24.B17 protein LpxC + H2O - Aquifex aeolicus ? - ? 367591 3.4.24.B17 protein LpxC + H2O - Thermotoga maritima ? - ? 367591 3.4.24.B17 protein LpxC + H2O cytoplasmic regulatory protein Escherichia coli ? - ? 367591 3.4.24.B17 protein LpxC + H2O essential for cell viability, enzyme controls the steady-state level of the LpxC protein, which has a key regulatory role in the biosynthesis of lipopolysaccharides Escherichia coli ? - ? 367591 3.4.24.B17 protein LpxC + H2O protein LpxC is UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase Escherichia coli ? - ? 367591 3.4.24.B17 protein LpxC + H2O turnover of LpxC requires a length- and sequence-specific C-terminal degradation signal. LpxC proteins from Salmonella enterica, Yersinia pseudotuberculosis, and Vibrio cholerae are degraded with half-lives comparable to the half-life of Escherichia coli LpxC (8 to 12 min). LpxC from Pseudomonas aeruginosa is degraded slowly with a half-life of 78 min. LpxC proteins from Agrobacterium tumefaciens and Rhodobacter capsulatus are degraded with half-lives of about 68 min and 20 min, respectively Escherichia coli ? - ? 367591 3.4.24.B17 protein LpxC + H2O turnover of LpxC requires a length- and sequence-specific C-terminal degradation signal. LpxC proteins from Salmonella enterica, Yersinia pseudotuberculosis, and Vibrio cholerae are degraded with half-lives comparable to the half-life of Escherichia coli LpxC (8 to 12 min). LpxC from Pseudomonas aeruginosa is degraded slowly with a half-life of 78 min. LpxC proteins from Agrobacterium tumefaciens and Rhodobacter capsulatus are degraded with half-lives of about 68 min and 20 min, respectively Escherichia coli W3110 / ATCC 27325 ? - ? 367591 3.4.24.B17 protein LpxC + H2O protein LpxC is UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase Escherichia coli W3110 / ATCC 27325 ? - ? 367591 3.4.24.B17 protein MgtC + H2O - Thermus thermophilus ? - ? 421237 3.4.24.B17 protein MgtC + H2O - Helicobacter pylori ? - ? 421237 3.4.24.B17 protein MgtC + H2O - Aquifex aeolicus ? - ? 421237 3.4.24.B17 protein MgtC + H2O - Thermotoga maritima ? - ? 421237 3.4.24.B17 protein P22 Arc repressor tagged with 108 motif tail + H2O tail specific degradation Escherichia coli ? - ? 367608 3.4.24.B17 protein P22 Arc repressor tagged with SsrA tail + H2O tail specific degradation Escherichia coli ? - ? 367607 3.4.24.B17 protein RpoH + H2O - Thermus thermophilus ? - ? 421238 3.4.24.B17 protein RpoH + H2O - Helicobacter pylori ? - ? 421238 3.4.24.B17 protein RpoH + H2O - Aquifex aeolicus ? - ? 421238 3.4.24.B17 protein RpoH + H2O - Thermotoga maritima ? - ? 421238 3.4.24.B17 protein SecY + H2O - Thermus thermophilus ? - ? 367584 3.4.24.B17 protein SecY + H2O - Escherichia coli ? - ? 367584 3.4.24.B17 protein SecY + H2O - Helicobacter pylori ? - ? 367584 3.4.24.B17 protein SecY + H2O - Aquifex aeolicus ? - ? 367584 3.4.24.B17 protein SecY + H2O - Thermotoga maritima ? - ? 367584 3.4.24.B17 protein SecY + H2O substrate protein is a subunit of protein translocase, failed to assemble with its partner SecE Escherichia coli ? - ? 367584 3.4.24.B17 protein SecY + H2O uncomplexed subunit of the protein translocase Escherichia coli ? - ? 367584 3.4.24.B17 protein SecY + H2O degradation of membrane protein, essentially required as a membrane-integrated quality control Escherichia coli ? - ? 367584 3.4.24.B17 protein SecY + H2O quality control, the enzyme regulates the amount of SecY assembled in the mitochondrial membrane, elimination of uncomplexed SecY is important for optimum protein translocation and for the integrity of the membrane Escherichia coli ? - ? 367584 3.4.24.B17 protein SecY + H2O SecY protein is a subunit of protein translocase Escherichia coli ? - ? 367584 3.4.24.B17 protein SecY + H2O - Escherichia coli W3110 / ATCC 27325 ? - ? 367584 3.4.24.B17 protein sigma 32 + H2O - Escherichia coli ? - ? 393366 3.4.24.B17 protein sigma 32 + H2O - Helicobacter pylori ? - ? 393366 3.4.24.B17 protein sigma32 + H2O - Escherichia coli ? - ? 367587 3.4.24.B17 protein sigma32 + H2O - Corynebacterium glutamicum ? - ? 367587 3.4.24.B17 protein sigma32 + H2O regulatory protein Escherichia coli ? - ? 367587 3.4.24.B17 protein sigma32 + H2O cytoplasmic regulatory protein Escherichia coli ? - ? 367587 3.4.24.B17 protein sigma32 + H2O heat shock factor Escherichia coli ? - ? 367587 3.4.24.B17 protein sigma32 + H2O no activity with the isolated 21 amino acid peptide from the C-terminus, heat shock promotor-specific subunit of RNA polymerase, wild-type and C-terminally truncated, up to 21 amino acid residues, protein, the substrates C-terminus is not required for activity or recognition and binding Escherichia coli ? - ? 367587 3.4.24.B17 protein sigma32 + H2O wild-type enzyme and fusion mutants MF1-4, not MF5, substrate protein is a heat-shock transcription factor, sigma32-C-his Escherichia coli ? - ? 367587 3.4.24.B17 protein sigma32 + H2O degradation has regulatory function Escherichia coli ? - ? 367587 3.4.24.B17 protein sigma32 + H2O protein sigma32 is also called RhoP Escherichia coli ? - ? 367587 3.4.24.B17 protein sigma32 + H2O - Escherichia coli TYE024 ? - ? 367587 3.4.24.B17 protein sigma32 + H2O protein sigma32 is also called RhoP Escherichia coli W3110 / ATCC 27325 ? - ? 367587 3.4.24.B17 protein sigma32 + H2O regulatory protein Escherichia coli peptides approximately 10 peptides with MW below 3 kDa ? 367605 3.4.24.B17 protein Spo0M + H2O - Bacillus subtilis ? - ? 421239 3.4.24.B17 protein Spo0M + H2O - Bacillus subtilis 168 ? - ? 421239 3.4.24.B17 protein translocation subunit SecY + H2O - Mycobacterium tuberculosis ? - ? 385631 3.4.24.B17 protein YccA + H2O - Thermus thermophilus ? - ? 367585 3.4.24.B17 protein YccA + H2O - Escherichia coli ? - ? 367585 3.4.24.B17 protein YccA + H2O - Helicobacter pylori ? - ? 367585 3.4.24.B17 protein YccA + H2O - Aquifex aeolicus ? - ? 367585 3.4.24.B17 protein YccA + H2O - Thermotoga maritima ? - ? 367585 3.4.24.B17 protein YccA + H2O degradation of membrane protein, essentially required as a membrane-integrated quality control Escherichia coli ? - ? 367585 3.4.24.B17 protein YccA + H2O - Escherichia coli W3110 / ATCC 27325 ? - ? 367585 3.4.24.B17 PspC protein + H2O - Escherichia coli ? - ? 432918 3.4.24.B17 PspC protein + H2O - Escherichia coli W3110 / ATCC 27325 ? - ? 432918 3.4.24.B17 RpoH protein + H2O enzyme recognizes the internal RpoH protein region, N- and C-terminus have no or only marginal effect on substrate binding Escherichia coli ? - ? 367596 3.4.24.B17 SecY protein + H2O - Escherichia coli ? - ? 367594 3.4.24.B17 SecY protein + H2O - Escherichia coli W3110 / ATCC 27325 ? - ? 367594 3.4.24.B17 sigma32 + H2O - Escherichia coli ? - ? 367592 3.4.24.B17 sigma32 + H2O hydrolyzes about 140 ATP molecules during the degradation of a single molecule of cy2-sigma32. Degradation of sigma32 proceeds from the N-terminus to the C-terminus Escherichia coli ? - ? 367592 3.4.24.B17 sigma32 + H2O ATP- and Zn2+-dependent degradation Escherichia coli ? - ? 367592 3.4.24.B17 SoxS + H2O - Escherichia coli ? - ? 385742 3.4.24.B17 ssrA-tagged Arc + H2O Arc, i.e. a soluble model substrate Escherichia coli ? - ? 450662 3.4.24.B17 ssrA-tagged ProW1-182 + H2O substrate is a truncated variant of multiple-pass inner-membrane protein ProW Escherichia coli ? - ? 450663 3.4.24.B17 subunit a of F0 + H2O F0 part of the proton ATPase Escherichia coli ? - ? 367603 3.4.24.B17 subunit alpha of ATPase F0F1 + H2O - Helicobacter pylori ? - ? 393553 3.4.24.B17 succinate dehydrogenase A + H2O - Corynebacterium glutamicum ? - ? 393562 3.4.24.B17 succinate dehydrogenase B + H2O - Corynebacterium glutamicum ? - ? 393563 3.4.24.B17 succinate dehydrogenase CD + H2O - Corynebacterium glutamicum ? - ? 393564 3.4.24.B17 superoxide response protein + H2O - Thermus thermophilus ? - ? 421565 3.4.24.B17 superoxide response protein + H2O - Helicobacter pylori ? - ? 421565 3.4.24.B17 superoxide response protein + H2O - Aquifex aeolicus ? - ? 421565 3.4.24.B17 superoxide response protein + H2O - Thermotoga maritima ? - ? 421565 3.4.24.B17 UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase + H2O - Escherichia coli ? - ? 393780 3.4.24.B17 UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase + H2O - Escherichia coli W3110 / ATCC 27325 ? - ? 393780 3.4.24.B17 uncomplexed form of the subunit alpha of the proton ATPase F0 + H2O - Escherichia coli ? - ? 393808 3.4.24.B17 uncomplexed form of the subunit alpha of the proton ATPase F0 + H2O - Escherichia coli W3110 / ATCC 27325 ? - ? 393808 3.4.24.B17 unfolded alpha-casein + H2O - Thermus thermophilus peptides between 10 and 30 kDa, no large intermediates ? 367613 3.4.24.B17 unfolded alpha-casein + H2O - Thermus thermophilus HB8 / ATCC 27634 / DSM 579 peptides between 10 and 30 kDa, no large intermediates ? 367613 3.4.24.B17 unfolded pepsin + H2O cleavage of small peptides from the C-terminal side of hydrophobic residues, no large intermediates Thermus thermophilus peptides between 10 and 30 kDa, no large intermediates ? 367614 3.4.24.B17 unfolded pepsin + H2O cleavage of small peptides from the C-terminal side of hydrophobic residues, no large intermediates Thermus thermophilus HB8 / ATCC 27634 / DSM 579 peptides between 10 and 30 kDa, no large intermediates ? 367614 3.4.24.B17 unstable derivatives of the N-terminal domain of the lambdacI repressor + H2O 3 derivatives with a nonpolar pentapeptide tail, i.e. cI104, cI105, cI108, and 1 with the SsrAtag, i.e. cI-SsrA Escherichia coli ? - ? 367611 3.4.24.B17 YccA protein + H2O - Escherichia coli ? - ? 432982 3.4.24.B17 YccA protein + H2O - Escherichia coli W3110 / ATCC 27325 ? - ? 432982 3.4.24.B17 YccA-(P3)-PhoA + H2O recombinant fusion protein Escherichia coli ? - ? 367604 3.4.24.B17 YfgM protein + H2O - Escherichia coli ? - ? 432983