3.4.24.82 5-carboxyfluorescein-Ala-Glu-Leu-Gln-Gly-Arg-Pro-Ile-Ser-Ile-Ala-Lys-N,N,N',N'-tetramethyl-6-carboxyrhodamine + H2O - Homo sapiens 5-carboxyfluorescein-Ala-Glu + Leu-Gln-Gly-Arg-Pro-Ile-Ser-Ile-Ala-Lys-N,N,N',N'-tetramethyl-6-carboxyrhodamine - ? 449364 3.4.24.82 Abz-TEGEARGSVI-Dap(2,4-dinitrophenyl)-KK + H2O - Homo sapiens ? - ? 391107 3.4.24.82 aggrecan + H2O - Homo sapiens ? - ? 15923 3.4.24.82 aggrecan + H2O 100 microg/ml Homo sapiens ? - ? 15923 3.4.24.82 aggrecan + H2O 2-8 microg, assay at pH 7.2, 37°C, reaction stopped by addition of 21 mM EDTA Homo sapiens ? - ? 15923 3.4.24.82 aggrecan + H2O 2mg/ml, assay at 37°C, 16 h Homo sapiens ? - ? 15923 3.4.24.82 aggrecan + H2O 40 microg Mus musculus ? - ? 15923 3.4.24.82 aggrecan + H2O ADAMTS-4 and ADAMTS-5 are the proteases responsible for the endogenous aggrecanase activity observed in IL-1beta stimulated bovine cartilage Homo sapiens ? - ? 15923 3.4.24.82 aggrecan + H2O cleavage at the Glu373-Ala374 site of aggrecan is measured Homo sapiens ? - ? 15923 3.4.24.82 aggrecan + H2O non-conservative mutation at P2' position of the bovine aggrecan (R375L) does not result in an observable difference in ADAMTS-4 susceptibility. Mutations in recombinant bovine aggrecan at Ser377 to Thr or Asn do not result in any significant difference in ADAMTS-4 cleavage. The S377Q mutation results in a significant reduction of cleavage by ADAMTS-4. The S377Q mutant is susceptible to cleavage by the C-terminally truncated ADAMTS-4. The S377A mutant is cleaved similarly to wild-type aggrecan Homo sapiens ? - ? 15923 3.4.24.82 aggrecan + H2O - Mus musculus fragments of aggrecan - ? 355668 3.4.24.82 aggrecan + H2O - Homo sapiens fragments of aggrecan - ? 355668 3.4.24.82 aggrecan + H2O - Rattus norvegicus fragments of aggrecan - ? 355668 3.4.24.82 aggrecan + H2O - Sus scrofa fragments of aggrecan - ? 355668 3.4.24.82 aggrecan + H2O - Bos taurus fragments of aggrecan - ? 355668 3.4.24.82 aggrecan + H2O Glu373-Ala374 is the major cleavage site, aggrecanase also cleaves at Glu1971-Leu1972, which is located in the gap region in the chondroitin sulfate attachment region, aggrecanase does not cleave at the matrix metalloproteinase site Asn341-Phe342 Bos taurus fragments of aggrecan - ? 355668 3.4.24.82 aggrecan + H2O preferred cleavage sites of aggrecanase-1 in descending order: E1667-G1668, E1771-A1772, E1871-L1872, E1480-G1481 and E373-A374 Homo sapiens fragments of aggrecan - ? 355668 3.4.24.82 aggrecan + H2O aggrecanase-1 does not cleave type II collagen, Tsp, fibronectin, casein or gelatin Homo sapiens fragments of aggrecan - ? 355668 3.4.24.82 aggrecan + H2O ADAMTS-5, i.e. aggrecanase-2 cleaves at Glu373-Ala374 Homo sapiens fragments of aggrecan - ? 355668 3.4.24.82 aggrecan + H2O ADAMTS-4 cleaves primarly at the Glu373-Ala374, but also, slowly and secondarily, at the Asn341-Phe342 site Homo sapiens fragments of aggrecan - ? 355668 3.4.24.82 aggrecan + H2O the thrombospondin type-1 motif located within the C-terminus of aggrecanase-1 is critical for substrate binding and cleavage Homo sapiens fragments of aggrecan - ? 355668 3.4.24.82 aggrecan + H2O both ADAMTS-1 and ADAMTS-4 cleave aggrecan at the Glu441-Ala442 bond Homo sapiens fragments of aggrecan - ? 355668 3.4.24.82 aggrecan + H2O cleavage at the Glu373-Ala374 site Mus musculus fragments of aggrecan - ? 355668 3.4.24.82 aggrecan + H2O cleaves the Glu373-Ala374 bond Mus musculus fragments of aggrecan - ir 355668 3.4.24.82 aggrecan + H2O cleaves the Glu373-Ala374 bond Homo sapiens fragments of aggrecan - ir 355668 3.4.24.82 aggrecan + H2O cleaves the Glu373-Ala374 bond Sus scrofa fragments of aggrecan - ir 355668 3.4.24.82 aggrecan + H2O cleaves the Glu373-Ala374 bond Bos taurus fragments of aggrecan - ir 355668 3.4.24.82 aggrecan + H2O aggrecanase-1 is responsible for the cleavage of aggrecan interglobular domain at the Glu373-Ala374 peptide bond Homo sapiens fragments of aggrecan - ? 355668 3.4.24.82 aggrecan + H2O - Mus musculus C3H/HEN fragments of aggrecan - ? 355668 3.4.24.82 aggrecan + H2O assay at pH 7.5, 16 h, 37°C, 100 nM ADAMTS4, reaction stopped by addition of Na acetate, Tris and EDTA Homo sapiens G1-NITEGE374 + ? - ? 404246 3.4.24.82 aggrecan-interglobular domain + H2O an aggrecan peptide with the N-terminal sequence ARGSVIL is released and quantified Homo sapiens ? - ? 411583 3.4.24.82 aggrecan-interglobular domain + H2O cleavage site Glu373-Ala374 in aggrecan–interglobular domain Homo sapiens ? - ? 411583 3.4.24.82 amyloid beta 1-38 peptide + H2O the enzyme cleaves after Glu4 and Glu12 Mus musculus amyloid beta 4-38 fragment + amyloid beta 12-38 fragment + ? - ? 448429 3.4.24.82 amyloid beta 1-40 peptide + H2O the enzyme cleaves after Glu4 and Glu12 Mus musculus amyloid beta 4-40 fragment + amyloid beta 12-40 fragment - ? 448430 3.4.24.82 amyloid beta 1-42 peptide + H2O the enzyme cleaves after Glu4 and Glu12 Mus musculus amyloid beta 4-42 fragment + amyloid beta 12-42 fragment + ? - ? 448431 3.4.24.82 biglycan + H2O - Homo sapiens ? - ? 391508 3.4.24.82 biglycan + H2O - Homo sapiens ? ADAMTS-4 and its substrate biglycan are involved in tubulogenesis by endothelial cells ? 391508 3.4.24.82 brevican + H2O - Homo sapiens fragments of brevican - ? 355670 3.4.24.82 brevican + H2O recombinantrat brevican, recombinant aggrecanase-1 most probably cleaves at Glu395-Ser396 Homo sapiens fragments of brevican - ? 355670 3.4.24.82 brevican + H2O - Homo sapiens ? - ? 367547 3.4.24.82 brevican + H2O - Bos taurus ? - ? 367547 3.4.24.82 brevican + H2O - Rattus norvegicus ? - ? 367547 3.4.24.82 brevican + H2O assay at 37°C, 3 h, 25 nM recombinant ADAMTS Homo sapiens ? - ? 367547 3.4.24.82 C6-(Gly-Pro-Hyp-Pro-Hyp-Gly)2-Gly-Pro-Hyp-Gly-Thr-Lys(Mca)-Gly-Glu-Leu-Glu-Gly-Arg-Gly-Thr-Lys(Dnp)-Gly-Ile-Ser-(Gly-Pro-Hyp-Pro-Hyp-Gly)2-Gly-Pro-Hyp-NH2 + H2O - Homo sapiens ? - ? 391547 3.4.24.82 carboxyfluorescein-AELQGRPISIAK-N,N,N',N'-tetramethyl-6-carboxyrhodamine + H2O - Homo sapiens carboxyfluorescein-AE + LQGRPISIAK-N,N,N',N'-tetramethyl-6-carboxyrhodamine - ? 391570 3.4.24.82 carboxyfluorescein-Ala-Glu-Leu-Asn-Gly-Arg-Pro-Ile-Ser-Ile-Ala-Lys-N,N,N',N'-tetramethyl-6-carboxyrhodamine + H2O - Homo sapiens carboxyfluorescein-Ala-Glu + Leu-Asn-Gly-Arg-Pro-Ile-Ser-Ile-Ala-Lys-N,N,N',N'-tetramethyl-6-carboxyrhodamine - ? 411844 3.4.24.82 carboxymethylated transferrin + H2O - Homo sapiens ? - ? 367657 3.4.24.82 carboxymethylated transferrin + H2O little activity Homo sapiens ? - ? 367657 3.4.24.82 cartilage disc extract supernantant + H2O - Homo sapiens hyaluron + hyaladherins - ? 384412 3.4.24.82 cartilage oligomeric matrix protein + H2O - Homo sapiens ? - ? 419381 3.4.24.82 chondroitin 4-sulfate + H2O - Homo sapiens ? - ? 391657 3.4.24.82 decorin + H2O - Homo sapiens ? - ? 384574 3.4.24.82 decorin + H2O - Sus scrofa ? - ? 384574 3.4.24.82 DVQEFRGVTAVIR + H2O - Homo sapiens ? - ? 391894 3.4.24.82 FAM-AELQGRPISIAK-TAMRA + H2O activity measured using a quenched fluorescent substrate Homo sapiens ? - ? 412041 3.4.24.82 fibromodulin + H2O - Homo sapiens ? - ? 383159 3.4.24.82 fibromodulin + H2O - Sus scrofa ? - ? 383159 3.4.24.82 fibromodulin + H2O cleaves at the Tyr44-Ala45 bond Homo sapiens ? - ? 383159 3.4.24.82 Fibronectin + H2O - Homo sapiens ? - ? 15394 3.4.24.82 fibulin-2 + H2O - Homo sapiens ? - ? 449920 3.4.24.82 hevin + H2O digestion of hevin by ADAMTS4 in vitro produces fragments similar to those present in brain lysates. The proteolysis liberates a hevin peptide, termed SPARC-like fragment, with significant homology to SPARC (secreted protein acidic and rich in cysteine) Mus musculus ? - ? 412157 3.4.24.82 HNEFRQRETYMVF + H2O - Homo sapiens ? - ? 392221 3.4.24.82 K(6-(fluorescein-5-carboxamido)-hexanoic acid)-DVQEFRGVTAVIR-C(QSY9)-KGK + H2O - Mus musculus C(QSY9) + KGK + K(6-(fluorescein-5-carboxamido)-hexanoic acid)-DVQEFRGVTAVIR - ? 392302 3.4.24.82 Lys(7-methoxycoumarin-4-yl)-Lys-Gln-Glu-Phe-Arg-Gly-Gln-Thr-Lys(Dnp)-NH2 + H2O - Sus scrofa ? - ? 412390 3.4.24.82 Lys(carboxyfluorescein)-Asp-Val-Gln-Glu-Phe-Arg-Gly-Val-Thr-Ala-Val-Ile-Arg-Lys(tetramethylrhodamine)-Lys-Gly-Lys-NH2 + H2O - Sus scrofa ? - ? 412391 3.4.24.82 matrilin-2 + H2O - Homo sapiens ? substrate contains two putative cleavage sites. The first site is located between residues D851 and L852 in the middle of the domain and the second at the boundary with the coiled-coil domain at the C-terminus. Deletion of the entire unique domain eliminates the proteolysis of matrilin-2. The first cleavage site is present in all matrilin-2 oligomers, the second cleavage site becomes apparent only in the matrilin-2 hetero-oligomers with matrilin-1 or matrilin-3 ? 433919 3.4.24.82 matrilin-3 + H2O cleaves at Glu435/Ala436 generating two species of 45 and 5 kDa Homo sapiens ? - ? 392568 3.4.24.82 additional information a disintegrin and metalloproteinase with thrombospondin motifs-4, aggrecanase 1 Homo sapiens ? - ? 89 3.4.24.82 additional information recombinant protein substrate based on the IGD (interglobular domain) of aggrecan, gst-IGD-flag Homo sapiens ? - ? 89 3.4.24.82 additional information ADAMTS-4 binds to vascular endothelial growth factor Homo sapiens ? - ? 89 3.4.24.82 N-procollagen + H2O - Bos taurus ? - ? 385320 3.4.24.82 neurocan + H2O - Rattus norvegicus ? - ? 434064 3.4.24.82 phosphacan + H2O - Rattus norvegicus ? - ? 434125 3.4.24.82 poly-ADP ribose polymerase-1 + H2O - Mus musculus ? - ? 448848 3.4.24.82 porcine aggrecan + H2O - Homo sapiens ? - ? 450482 3.4.24.82 QTVTWPDMELPLPRNITEGEARGSVILTVKPIFEVSPSPLK + H2O - Bos taurus QTVTWPDMELPLPRNITEGE + ARGSVILTVKPIFEVSPSPLK - ? 355672 3.4.24.82 recombinant Agg1 + H2O - Bos taurus fragments of recombinant Agg1 - ? 355669 3.4.24.82 recombinant Agg1 + H2O rAGG1 is composed of the complete interglobular domain, i.e. IGD, of human aggrecan, flanked by the marker sequences FLAG at the amino terminus and human immunglobulin G1 constant region at the C-terminus Rattus norvegicus fragments of recombinant Agg1 - ? 355669 3.4.24.82 transferrin + H2O - Sus scrofa ? - ? 115529 3.4.24.82 versican + H2O both ADAMTS-1 and ADAMTS-4 cleave native human and recombinant versican at Glu441-A442, ADAMTS-4 is 5-10fold more active than ADAMTS-1 Homo sapiens fragments of versican - ? 355671 3.4.24.82 versican + H2O - Homo sapiens ? - ? 367541 3.4.24.82 versican + H2O - Mus musculus ? - ? 367541 3.4.24.82 versican V1 + H2O - Homo sapiens fragments of versican V1 - ? 419627 3.4.24.82 versican V2 + H2O - Homo sapiens glial hyaluronate binding protein + ? - ? 385911 3.4.24.82 versican-1 + H2O - Bos taurus ? - ? 385912 3.4.24.82 versican-2 + H2O - Bos taurus ? - ? 385913 3.4.24.82 VQTVTWPDMELPLPRNITEGEARGSVILTVKPIFEVSPSPLKG + H2O - Homo sapiens ? - ? 434464 3.4.24.82 VQTVTWPDMELPLPRNITEGEARGSVILTVKPIFEVSPSPLKG + H2O 43mer peptide substrate Homo sapiens ? - ? 434464 3.4.24.82 [C6-(Gly-Pro-Hyp)5-Gly-Thr-Lys(7-amido-4-methylcoumarin)-Gly-Glu-Leu-Glu-Gly-Arg-Gly-Thr-Lys(2,4-dinitrophenyl)-Gly-Ile-Ser-(Gly-Pro-Hyp)5-NH2]3 + H2O - Homo sapiens ? - ? 393897