3.4.23.39 4-(4-dimethylaminophenylazo)benzoyl-CH2-CH2-CO-ALERMFLSFP-diaminopimelate-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid + H2O - Plasmodium falciparum ? - ? 383873 3.4.23.39 4-(4-dimethylaminophenylazo)benzoyl-CH2-CH2-CO-CO-ALERMFLSFP-diaminopimelate-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid + H2O - Plasmodium falciparum ? - ? 453157 3.4.23.39 4-(4-dimethylaminophenylazo)benzoyl-CH2-CH2-CO-ERMFLSFP-diaminopimelate-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid + H2O - Plasmodium falciparum ? - ? 383874 3.4.23.39 4-[[4'-(dimethylamino)phenyl]azo]-benzoyl-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-(gamma-aminobutyryl)-5-[(2'-aminoethyl)-amino]naphthalenesulfonic acid + H2O - Plasmodium falciparum ? - ? 363166 3.4.23.39 4-[[4'-(dimethylamino)phenyl]azo]-benzoyl-Glu-Arg-Nle-Phe-Leu-Ser-Phe-Pro-5-[(2'-aminoethyl)-amino]naphthalenesulfonic acid + H2O - Plasmodium falciparum ? - ? 363165 3.4.23.39 Abz-Thr-Ile-Nle-(4-nitro)Phe-Gln-Arg-NH2 + H2O - Plasmodium falciparum Abz-Thr-Ile-Nle + (4-nitro)Phe-Gln-Arg-NH2 - ? 413118 3.4.23.39 actin + H2O - Plasmodium falciparum ? - ? 363163 3.4.23.39 Ala-Leu-Glu-Arg-Nle-Phe-Phe(NO2)-Ser-Phe-Pro + H2O - Plasmodium falciparum Ala-Leu-Glu-Arg-Nle-Phe + Phe(NO2)-Ser-Phe-Pro - ? 453158 3.4.23.39 Ala-Leu-Glu-Arg-Thr-Phe-Leu-Ser-Phe-Pro-Thr + H2O - Plasmodium falciparum Ala-Leu-Glu-Arg-Thr-Phe + Leu-Ser-Phe-Pro-Thr - ? 363158 3.4.23.39 Ala-Leu-Glu-Arg-Thr-Phe-Phe(NO2)-Ser-Phe-Pro-Thr + H2O - Plasmodium falciparum Ala-Leu-Glu-Arg-Thr-Phe + Phe(NO2)-Ser-Phe-Pro-Thr - ? 363167 3.4.23.39 Ala-Leu-Glu-Arg-Thr-Phe-Phe(p-NO2)-Ser-Phe-Pro-Thr + H2O - Plasmodium falciparum Ala-Leu-Glu-Arg-Thr-Phe + Phe(p-NO2)-Ser-Phe-Pro-Thr - ? 404251 3.4.23.39 Arg-Gln-Phe-Phe(p-NO2)-Ile-Thr + H2O - Plasmodium falciparum Arg-Gln-Phe + Phe(p-NO2)-Ile-Thr - ? 404353 3.4.23.39 DABCYL-Glu-Arg-Nle-Phe-Leu-Ser-Phe-Pro-EDANS + H2O FRET substrate, substrate is designed to mimic the hemoglobin cleavage site. The substrate is conjugated with the fluorescent donor EDANS and the quencher DABCYL Plasmodium falciparum ? - ? 384561 3.4.23.39 DABCYL-L-Glu-L-Arg-L-Nle-L-Phe-L-Leu-L-Ser-L-Phe-L-Pro-EDANS + H2O FRET-based substrate Plasmodium falciparum ? - ? 418572 3.4.23.39 EDANS-CO-CH2-CH2-CO-Ala-Leu-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-Dap-(DABCYL)-OH + H2O - Plasmodium falciparum ? - ? 391900 3.4.23.39 EDANS-CO-CH2-CH2-CO-Ala-Leu-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-Dap-(DABCYL)-OH + H2O quenched fluorescent peptide substrate Plasmodium falciparum ? - ? 391900 3.4.23.39 EDANS-CO-CH2-CH2-CO-L-Ala-L-Leu-L-Glu-L-Arg-L-Met-L-Phe-L-Leu-L-Ser-L-Phe-L-Pro-Dap(DABCYL)-OH + H2O - Plasmodium falciparum ? - ? 418596 3.4.23.39 EDANS-CO-CH2-CH2-COALERMFLSFP-diaminopimelate-(DABCYL)OH + H2O - Plasmodium falciparum ? - ? 412024 3.4.23.39 GST-SH3 peptide + H2O Y-/-V and L-/-L are major cleavage sites Plasmodium falciparum ? - ? 363203 3.4.23.39 Hemoglobin + H2O - Plasmodium falciparum ? - ? 15450 3.4.23.39 Hemoglobin + H2O - Plasmodium sp. ? - ? 15450 3.4.23.39 Hemoglobin + H2O enzyme is involved in initial steps of Plasmodium hemoglobin degradation Plasmodium falciparum ? - ? 15450 3.4.23.39 Hemoglobin + H2O during the infection of erythrocytes by the human malaria parasite Plasmodium falciparum, up to 80% of the host cell hemoglobin is degraded to provide amino acids for parasite nutrition. Plasmepsin I and plasmepsin II are believed to be responsible for initiating the catabolic process Plasmodium falciparum ? - ? 15450 3.4.23.39 Hemoglobin + H2O the enzyme initiates degradation of hemoglobin by cleavage at the alpha-chain between Phe33 and Leu34 Plasmodium falciparum ? - ? 15450 3.4.23.39 Hemoglobin + H2O the enzyme participates in the hemoglobin digestive pathway Plasmodium falciparum ? - ? 15450 3.4.23.39 Hemoglobin + H2O the enzyme is involved in degradation of the host cell hemoglobin within the acidic food vacuole of the parasite Plasmodium falciparum ? - ? 15450 3.4.23.39 Hemoglobin + H2O pro-plasmepsin II is transported through the secretory system to cytostomal vacuoles and then is carried along with its substrate hemoglobin to the food vacuole where it is proteolytically processed to mature PMII Plasmodium falciparum ? - ? 15450 3.4.23.39 Hemoglobin + H2O cleaves native hemoglobin and loosely folded alpha-chain Plasmodium falciparum ? - ? 15450 3.4.23.39 Hemoglobin + H2O bovine and human hemoglobin are used Plasmodium falciparum ? - ? 15450 3.4.23.39 Hemoglobin + H2O the enzyme participates in the hemoglobin digestive pathway Plasmodium falciparum HB3 ? - ? 15450 3.4.23.39 hemoglobin alpha-chain + H2O cleavage sites: Phe33-/-Leu34, Thr108-/-Leu109, Leu136-/-Thr137 Plasmodium falciparum ? - ? 363148 3.4.23.39 hemoglobin beta-chain + H2O cleavage site: Leu32-/-Val33 Plasmodium falciparum ? - ? 65046 3.4.23.39 Leu-Glu-Arg-Ile-Phe-Phe(NO2)-Ser-Phe + H2O - Plasmodium falciparum Leu-Glu-Arg-Ile-Phe + Phe(NO2)-Ser-Phe - ? 363153 3.4.23.39 Leu-Glu-Arg-Val-Phe-Phe(NO2)-Ser-Phe + H2O - Plasmodium falciparum Leu-Glu-Arg-Val-Phe + Phe(NO2)-Ser-Phe - ? 363152 3.4.23.39 Leu-Ser-Phe(NO2)-Nle-Ala-Leu-OMe + H2O - Plasmodium falciparum ? - ? 37114 3.4.23.39 Lys-Ala-Ile-Glu-Phe-Phe(NO2)-Arg-Leu + H2O - Plasmodium falciparum Lys-Ala-Ile-Glu-Phe + Phe(NO2)-Arg-Leu - ? 363178 3.4.23.39 Lys-Asp-Ile-Glu-Phe-Phe(NO2)-Arg-Leu + H2O - Plasmodium falciparum Lys-Asp-Ile-Glu-Phe + Phe(NO2)-Arg-Leu - ? 363180 3.4.23.39 Lys-Glu-Leu-Val-Phe-Phe(NO2)-Ala-Leu-Lys + H2O - Plasmodium falciparum Lys-Glu-Leu-Val-Phe + Phe(NO2)-Ala-Leu-Lys - ? 363155 3.4.23.39 Lys-Glu-Phe-Asn-Phe-Phe(NO2)-Ala-Leu-Lys + H2O - Plasmodium falciparum Lys-Glu-Phe-Asn-Phe + Phe(NO2)-Ala-Leu-Lys - ? 363156 3.4.23.39 Lys-Glu-Phe-Ile-Phe-Phe(NO2)-Ala-Leu-Lys + H2O - Plasmodium falciparum Lys-Glu-Phe-Ile-Phe + Phe(NO2)-Ala-Leu-Lys - ? 453156 3.4.23.39 Lys-Glu-Phe-Val-Lys-Phe(NO2)-Ala-Leu-Lys + H2O - Plasmodium falciparum Lys-Glu-Phe-Val-Lys + Phe(NO2)-Ala-Leu-Lys + - ? 453159 3.4.23.39 Lys-Glu-Phe-Val-Lys-Phe(NO2)-Ala-Leu-Lys + H2O - Plasmodium falciparum ? - ? 454034 3.4.23.39 Lys-Glu-Phe-Val-Phe-4-nitrophenylalanine-Ala-Leu-Lys + H2O - Plasmodium falciparum ? - ? 412395 3.4.23.39 Lys-Glu-Phe-Val-Phe-Phe(NO2)-Ala-Leu-Lys + H2O - Plasmodium falciparum Lys-Glu-Phe-Val-Phe + Phe(NO2)-Ala-Leu-Lys - ? 363154 3.4.23.39 Lys-Glu-Phe-Val-Phe-Phe(NO2)-Arg-Leu-Lys + H2O - Plasmodium falciparum Lys-Glu-Phe-Val-Phe + Phe(NO2)-Arg-Leu-Lys - ? 363157 3.4.23.39 Lys-Lys-Ile-Glu-Phe-Phe(NO2)-Arg-Leu + H2O - Plasmodium falciparum Lys-Lys-Ile-Glu-Phe + Phe(NO2)-Arg-Leu - ? 363181 3.4.23.39 Lys-Phe-Ile-Glu-Phe-Phe(NO2)-Arg-Leu + H2O - Plasmodium falciparum Lys-Phe-Ile-Glu-Phe + Phe(NO2)-Arg-Leu - ? 363177 3.4.23.39 Lys-Pro-Ala-Glu-Phe-Phe(NO2)-Arg-Leu + H2O - Plasmodium falciparum Lys-Pro-Ala-Glu-Phe + Phe(NO2)-Arg-Leu - ? 363183 3.4.23.39 Lys-Pro-Asp-Glu-Phe-Phe(NO2)-Arg-Leu + H2O - Plasmodium falciparum Lys-Pro-Asp-Glu-Phe + Phe(NO2)-Arg-Leu - ? 363185 3.4.23.39 Lys-Pro-Ile-Ala-Phe-Phe(NO2)-Arg-Leu + H2O - Plasmodium falciparum Lys-Pro-Ile-Ala-Phe + Phe(NO2)-Arg-Leu - ? 363189 3.4.23.39 Lys-Pro-Ile-Asp-Phe-Phe(NO2)-Arg-Leu + H2O - Plasmodium falciparum Lys-Pro-Ile-Asp-Phe + Phe(NO2)-Arg-Leu - ? 363191 3.4.23.39 Lys-Pro-Ile-Glu-Phe-NO2Phe-Arg-Leu + H2O - Plasmodium falciparum Lys-Pro-Ile-Glu-Phe + NO2Phe-Arg-Leu - ? 450246 3.4.23.39 Lys-Pro-Ile-Glu-Phe-Phe(NO2)-Ala-Leu + H2O - Plasmodium falciparum Lys-Pro-Ile-Glu-Phe + Phe(NO2)-Ala-Leu - ? 363193 3.4.23.39 Lys-Pro-Ile-Glu-Phe-Phe(NO2)-Arg-Arg + H2O - Plasmodium falciparum Lys-Pro-Ile-Glu-Phe + Phe(NO2)-Arg-Arg - ? 363202 3.4.23.39 Lys-Pro-Ile-Glu-Phe-Phe(NO2)-Arg-Asp + H2O - Plasmodium falciparum Lys-Pro-Ile-Glu-Phe + Phe(NO2)-Arg-Asp - ? 363201 3.4.23.39 Lys-Pro-Ile-Glu-Phe-Phe(NO2)-Arg-Ile + H2O - Plasmodium falciparum Lys-Pro-Ile-Glu-Phe + Phe(NO2)-Arg-Ile - ? 363198 3.4.23.39 Lys-Pro-Ile-Glu-Phe-Phe(NO2)-Arg-Leu + H2O - Plasmodium falciparum Lys-Pro-Ile-Glu-Phe + Phe(NO2)-Arg-Leu - ? 363176 3.4.23.39 Lys-Pro-Ile-Glu-Phe-Phe(NO2)-Arg-Ser + H2O - Plasmodium falciparum Lys-Pro-Ile-Glu-Phe + Phe(NO2)-Arg-Ser - ? 363200 3.4.23.39 Lys-Pro-Ile-Glu-Phe-Phe(NO2)-Arg-Val + H2O - Plasmodium falciparum Lys-Pro-Ile-Glu-Phe + Phe(NO2)-Arg-Val - ? 363199 3.4.23.39 Lys-Pro-Ile-Glu-Phe-Phe(NO2)-Glu-Leu + H2O - Plasmodium falciparum Lys-Pro-Ile-Glu-Phe + Phe(NO2)-Glu-Leu - ? 363196 3.4.23.39 Lys-Pro-Ile-Glu-Phe-Phe(NO2)-Lys-Leu + H2O - Plasmodium falciparum Lys-Pro-Ile-Glu-Phe + Phe(NO2)-Lys-Leu - ? 363197 3.4.23.39 Lys-Pro-Ile-Glu-Phe-Phe(NO2)-Ser-Leu + H2O - Plasmodium falciparum Lys-Pro-Ile-Glu-Phe + Phe(NO2)-Ser-Leu - ? 363195 3.4.23.39 Lys-Pro-Ile-Glu-Phe-Phe(NO2)-Val-Leu + H2O - Plasmodium falciparum Lys-Pro-Ile-Glu-Phe + Phe(NO2)-Val-Leu - ? 363194 3.4.23.39 Lys-Pro-Ile-Leu-Phe-Nph-Arg-Leu + H2O chromogenic peptide substrate Plasmodium falciparum ? - ? 392508 3.4.23.39 Lys-Pro-Ile-Leu-Phe-Phe(NO2)-Arg-Leu + H2O - Plasmodium falciparum Lys-Pro-Ile-Leu-Phe + Phe(NO2)-Arg-Leu - ? 363187 3.4.23.39 Lys-Pro-Ile-Lys-Phe-Phe(NO2)-Arg-Leu + H2O - Plasmodium falciparum Lys-Pro-Ile-Lys-Phe + Phe(NO2)-Arg-Leu - ? 363170 3.4.23.39 Lys-Pro-Ile-Nle-Phe-Phe(NO2)-Arg-Leu + H2O - Plasmodium falciparum Lys-Pro-Ile-Nle-Phe + Phe(NO2)-Arg-Leu - ? 363188 3.4.23.39 Lys-Pro-Ile-Ser-Phe-Phe(NO2)-Arg-Leu + H2O - Plasmodium falciparum Lys-Pro-Ile-Ser-Phe + Phe(NO2)-Arg-Leu - ? 363190 3.4.23.39 Lys-Pro-Ile-Val-Phe-Phe(NO2)-Arg-Leu + H2O - Plasmodium falciparum Lys-Pro-Ile-Val-Phe + Phe(NO2)-Arg-Leu - ? 363169 3.4.23.39 Lys-Pro-Lys-Glu-Phe-Phe(NO2)-Arg-Leu + H2O - Plasmodium falciparum Lys-Pro-Lys-Glu-Phe + Phe(NO2)-Arg-Leu - ? 363186 3.4.23.39 Lys-Pro-Phe-Glu-Phe-Phe(NO2)-Arg-Leu + H2O - Plasmodium falciparum Lys-Pro-Phe-Glu-Phe + Phe(NO2)-Arg-Leu - ? 363182 3.4.23.39 Lys-Pro-Ser-Glu-Phe-Phe(NO2)-Arg-Leu + H2O - Plasmodium falciparum Lys-Pro-Ser-Glu-Phe + Phe(NO2)-Arg-Leu - ? 363184 3.4.23.39 Lys-Ser-Ile-Glu-Phe-Phe(NO2)-Arg-Leu + H2O - Plasmodium falciparum Lys-Ser-Ile-Glu-Phe + Phe(NO2)-Arg-Leu - ? 363179 3.4.23.39 additional information the enzyme has a preference for a small hydrophobic residue in the P3 position of the substrate. The S2 and S3 subsites of plasmepsin II are hydrophobic in nature and on this basis the hemoglobin-related substrate, with Arg and Thr in the P3 and P2 positions respectively may not represent the optimal substrate Plasmodium falciparum ? - ? 89 3.4.23.39 additional information multiple explicit-water molecular dynamics simulations of plasmepsin II, uncomplexed and with a hydroxypropylamine peptidomimetic inhibitor, indicate that protonation of Asp214 favours a stable active site structure Plasmodium sp. ? - ? 89 3.4.23.39 Myoglobin + H2O horse myoglobin is used Plasmodium falciparum ? - ? 16359 3.4.23.39 p47phox-PX + H2O PX domain of p47phox, a cytosolic factor of the neutrophil NADPH oxydase is digested Plasmodium falciparum ? - ? 412650 3.4.23.39 plasmepsin 2 + H2O PSM2 auto-cleavage after F112 is complete after 20 min in 10 mM citrate pH 4.5 Plasmodium falciparum ? - ? 410941 3.4.23.39 protein 4.1 + H2O - Plasmodium falciparum ? - ? 363164 3.4.23.39 spectrin + H2O - Plasmodium falciparum ? - ? 362513 3.4.23.39 spectrin + H2O recombinant plasmepsin II hydrolyzes native spectrin at pH 6.8 Plasmodium falciparum ? - ? 362513 3.4.23.39 spectrin + H2O the enzyme catalyzes the degradation of spectrin in the erythrocyte membrane cytoskeletron Plasmodium falciparum ? - ? 362513