3.4.23.18 angiotensin + H2O cleavage site: Tyr-Ile, not His-Pro, Aspergillus oryzae enzyme, cleavage specificity compared to pepsin and cathepsin D Aspergillus oryzae ? - ? 16268 3.4.23.18 angiotensin II + H2O cleavage site: Tyr4-Ile5 Aspergillus phoenicis ? - ? 16269 3.4.23.18 Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val-Tyr-Ser + H2O i.e. tetradecapeptide of a renin substrate, cleavage sites: Tyr-Ile, His-Pro, Leu-Val, Aspergillus oryzae enzyme, cleavage specificity compared to pepsin and cathepsin D Aspergillus oryzae ? - ? 16274 3.4.23.18 Benzyloxycarbonyl-Ala-Ala-Lys-Ala-Ala-Ala + H2O best substrate Aspergillus niger Benzyloxycarbonyl-Ala-Ala-Lys + Ala-Ala-Ala - ? 16284 3.4.23.18 Benzyloxycarbonyl-Ala-Ala-Phe-Phe 3-(4-pyridyl)propyl ester + H2O - Aspergillus oryzae Benzyloxycarbonyl-Ala-Ala-Phe + Phe 3-(4-pyridyl)propyl ester - ? 16286 3.4.23.18 Benzyloxycarbonyl-Ala-Lys-Ala-Ala-Ala + H2O - Aspergillus niger Benzyloxycarbonyl-Ala-Lys + Ala-Ala-Ala - ? 16283 3.4.23.18 Benzyloxycarbonyl-His-Phe-Phe ethyl ester + H2O - Aspergillus oryzae Benzyloxycarbonyl-His-Phe + Phe ethyl ester - ? 16285 3.4.23.18 Benzyloxycarbonyl-Lys-Ala-Ala + H2O poor substrate Aspergillus niger Benzyloxycarbonyl-Lys + Ala-Ala - ? 16279 3.4.23.18 Benzyloxycarbonyl-Lys-Ala-Ala + H2O poor substrate Aspergillus phoenicis Benzyloxycarbonyl-Lys + Ala-Ala - ? 16279 3.4.23.18 Benzyloxycarbonyl-Lys-Ala-Ala-Ala + H2O - Aspergillus niger Benzyloxycarbonyl-Lys + Ala-Ala-Ala - ? 16280 3.4.23.18 Benzyloxycarbonyl-Lys-Ala-Ala-Ala-Ala + H2O poor substrate Aspergillus niger Benzyloxycarbonyl-Lys + Ala-Ala-Ala-Ala - ? 16281 3.4.23.18 Benzyloxycarbonyl-Lys-Leu-Ala + H2O - Aspergillus niger Benzyloxycarbonyl-Lys + Leu-Ala - ? 16278 3.4.23.18 Benzyloxycarbonyl-Lys-Leu-Ala + H2O - Aspergillus phoenicis Benzyloxycarbonyl-Lys + Leu-Ala - ? 16278 3.4.23.18 Benzyloxycarbonyl-Lys-Leu-Ala + H2O - Aspergillus niger B Benzyloxycarbonyl-Lys + Leu-Ala - ? 16278 3.4.23.18 Benzyloxycarbonyl-Lys-Leu-Ala-Ala + H2O - Aspergillus niger Benzyloxycarbonyl-Lys + Leu-Ala-Ala - ? 16282 3.4.23.18 Benzyloxycarbonyl-Lys-Phe-Ala + H2O - Aspergillus niger Benzyloxycarbonyl-Lys + Phe-Ala - ? 16277 3.4.23.18 Benzyloxycarbonyl-Lys-Phe-Ala + H2O - Aspergillus phoenicis Benzyloxycarbonyl-Lys + Phe-Ala - ? 16277 3.4.23.18 Benzyloxycarbonyl-Lys-Phe-Ala + H2O - Aspergillus niger B Benzyloxycarbonyl-Lys + Phe-Ala - ? 16277 3.4.23.18 casein + H2O - Aspergillus niger ? - ? 15445 3.4.23.18 casein + H2O - Aspergillus oryzae ? - ? 15445 3.4.23.18 casein + H2O - Trichoderma harzianum ? - ? 15445 3.4.23.18 casein + H2O - Aspergillus pseudoglaucus ? - ? 15445 3.4.23.18 casein + H2O highest activity Aspergillus terreus ? - ? 15445 3.4.23.18 casein + H2O milk casein Aspergillus phoenicis ? - ? 15445 3.4.23.18 casein + H2O - Trichoderma harzianum GIM 3.442 ? - ? 15445 3.4.23.18 casein + H2O highest activity Aspergillus terreus gr. ? - ? 15445 3.4.23.18 casein + H2O milk casein Aspergillus phoenicis R-3813 ? - ? 15445 3.4.23.18 Chymotrypsinogen + H2O - Aspergillus phoenicis Pi-Chymotrypsin - ? 16267 3.4.23.18 Chymotrypsinogen + H2O cleavage site: Arg15-Ile16 Aspergillus oryzae Pi-Chymotrypsin - ? 16267 3.4.23.18 Chymotrypsinogen + H2O bovine chymotrypsinogen Aspergillus oryzae Pi-Chymotrypsin - ? 16267 3.4.23.18 Chymotrypsinogen + H2O - Aspergillus phoenicis R-3813 Pi-Chymotrypsin - ? 16267 3.4.23.18 chymotrypsinogen A + H2O activation Aspergillus phoenicis chymotrypsin + peptide fragment - ? 383099 3.4.23.18 chymotrypsinogen A + H2O activation Aspergillus phoenicis R-3813 chymotrypsin + peptide fragment - ? 383099 3.4.23.18 Cytochrome c + H2O - Aspergillus oryzae ? - ? 16271 3.4.23.18 Cytochrome c + H2O from horse heart Aspergillus oryzae ? - ? 16271 3.4.23.18 Cytochrome c + H2O - Aspergillus oryzae 365-U-64-1 ? - ? 16271 3.4.23.18 Dnp-Ala-Ala-Phe-Phe-Ala-Arg-NH2 + H2O chromogenic synthetic peptide substrate Aspergillus phoenicis Dnp-Ala-Ala-Phe + Phe-Ala-Arg-NH2 - ? 384642 3.4.23.18 DRVYIHPFHLLVYS + 3 H2O tetradecapeptide renin substrate, the enzyme cleaves the Xaa-pro bond with Xaa being any amino acid Aspergillus oryzae DRVY + Ile-His + PFHLL + VYS - ? 383132 3.4.23.18 DRVYIHPFHLLVYS + 3 H2O tetradecapeptide renin substrate, the enzyme cleaves the Xaa-pro bond with Xaa being any amino acid Aspergillus phoenicis DRVY + Ile-His + PFHLL + VYS - ? 383132 3.4.23.18 DRVYIHPFHLLVYS + 3 H2O tetradecapeptide renin substrate, the enzyme cleaves the Xaa-pro bond with Xaa being any amino acid Aspergillus sojae DRVY + Ile-His + PFHLL + VYS - ? 383132 3.4.23.18 DRVYIHPFHLLVYS + 3 H2O tetradecapeptide renin substrate, the enzyme cleaves the Xaa-pro bond with Xaa being any amino acid Aspergillus phoenicis R-3813 DRVY + Ile-His + PFHLL + VYS - ? 383132 3.4.23.18 DRVYIHPFHLLVYS + 3 H2O tetradecapeptide renin substrate, the enzyme cleaves the Xaa-pro bond with Xaa being any amino acid Aspergillus phoenicis DRVY + Ile-His + PFHLL + Val-Tyr-Ser - ? 383133 3.4.23.18 FVNQHLCGSHLVEALYLVCGERGFFYTPKA + 3 H2O substrate is the oxidized insulin B chain, primarily cleavage at Leu15-Tyr16 and Phe24-Phe25, and minor cleavage of Tyr16-Leu17, overview Aspergillus sojae FVNQHLCGSHLVEAL + Tyr + LVCGERGF + FYTPKA - ? 383164 3.4.23.18 FVNQHLCGSHLVEALYLVCGERGFFYTPKA + H2O substrate is the oxidized insulin B chain, cleavage site specificity, overview Aspergillus phoenicis FVNQHLCGSH + LVEA + Leu + Tyr + LVCGERGF + FYTPKA - ? 384735 3.4.23.18 Gelatin + H2O - Aspergillus terreus ? - ? 16570 3.4.23.18 HAP-01 + H2O - Aspergillus oryzae ? - ? 450029 3.4.23.18 Hemoglobin + H2O - Aspergillus niger ? - ? 15450 3.4.23.18 Hemoglobin + H2O - Aspergillus phoenicis ? - ? 15450 3.4.23.18 Hemoglobin + H2O - Aspergillus awamori ? - ? 15450 3.4.23.18 Hemoglobin + H2O - Aspergillus luchuensis ? - ? 15450 3.4.23.18 Hemoglobin + H2O best substrate Aspergillus fumigatus ? - ? 15450 3.4.23.18 Hemoglobin + H2O urea-denatured hemoglobin Aspergillus oryzae ? - ? 15450 3.4.23.18 Hemoglobin + H2O urea-denatured hemoglobin Aspergillus oryzae 365-U-64-1 ? - ? 15450 3.4.23.18 milk protein + H2O - Aspergillus pseudoglaucus ? - ? 95048 3.4.23.18 additional information specificity Aspergillus niger ? - ? 89 3.4.23.18 additional information specificity Aspergillus phoenicis ? - ? 89 3.4.23.18 additional information does not require a hydrophobic amino acid in P1, P2 or P3 position, prefers substrates with hydrophobic amino acid in P1', less so in P2' Aspergillus phoenicis ? - ? 89 3.4.23.18 additional information no milk clotting activity Aspergillus phoenicis ? - ? 89 3.4.23.18 additional information no hydrolysis of benzyloxycarbonyl-Lys-Gly-Ala, benzyloxycarbonyl-Lys-(D)-Leu-Ala Aspergillus niger ? - ? 89 3.4.23.18 additional information no hydrolysis of benzyloxycarbonyl-Lys-Gly-Ala, benzyloxycarbonyl-Lys-(D)-Leu-Ala Aspergillus phoenicis ? - ? 89 3.4.23.18 additional information the extracellular enzyme pays a role in development of aspergillosis in lung of mammalia, but it is not essential for virulence Aspergillus fumigatus ? - ? 89 3.4.23.18 additional information the enzyme prefers hydrophobic amino acid residues at P1 and P1', but also accepts Lys Aspergillus niger ? - ? 89 3.4.23.18 additional information the enzyme prefers hydrophobic amino acid residues at P1 and P1', but also accepts Lys Aspergillus oryzae ? - ? 89 3.4.23.18 additional information the enzyme prefers hydrophobic amino acid residues at P1 and P1', but also accepts Lys Aspergillus phoenicis ? - ? 89 3.4.23.18 additional information the enzyme prefers hydrophobic amino acid residues at P1 and P1', but also accepts Lys Aspergillus sojae ? - ? 89 3.4.23.18 additional information the enzyme prefers hydrophobic amino acid residues at P1 and P1', but also accepts Lys Aspergillus awamori ? - ? 89 3.4.23.18 additional information the enzyme prefers hydrophobic amino acid residues at P1 and P1', but also accepts Lys Aspergillus luchuensis ? - ? 89 3.4.23.18 additional information the enzyme prefers hydrophobic amino acid residues at P1 and P1', but also accepts Lys Aspergillus foetidus ? - ? 89 3.4.23.18 additional information the enzyme prefers hydrophobic amino acid residues at P1 and P1', but also accepts Lys, at pH 5.5 the enzyme shows also an elastolytic activity with elastin Congo red Aspergillus fumigatus ? - ? 89 3.4.23.18 additional information the enzyme prefers hydrophobic amino acid residues at P1 and P1', but also accepts Lys, no activity with Z-Glu-Tyr or Z-Tyr-Leu, no milk clotting Aspergillus phoenicis ? - ? 89 3.4.23.18 additional information the immediate turbidity levels obtained after treatments of the black currant juice samples with the Denapsin 2P preparation are in the low end of the range Aspergillus niger ? - ? 89 3.4.23.18 additional information the enzyme efficiently decolorises red-pigmented proteins during dried bonito fermentation. The enzyme is also able to decolorise flaked, dried bonito and to bleach a blood-stained cloth Aspergillus pseudoglaucus ? - ? 89 3.4.23.18 additional information the propeptide is necessary for catalytic activity Aspergillus pseudoglaucus ? - ? 89 3.4.23.18 additional information no milk clotting activity Aspergillus phoenicis R-3813 ? - ? 89 3.4.23.18 additional information the enzyme prefers hydrophobic amino acid residues at P1 and P1', but also accepts Lys Aspergillus phoenicis R-3813 ? - ? 89 3.4.23.18 additional information the enzyme prefers hydrophobic amino acid residues at P1 and P1', but also accepts Lys, no activity with Z-Glu-Tyr or Z-Tyr-Leu, no milk clotting Aspergillus phoenicis R-3813 ? - ? 89 3.4.23.18 additional information the enzyme efficiently decolorises red-pigmented proteins during dried bonito fermentation. The enzyme is also able to decolorise flaked, dried bonito and to bleach a blood-stained cloth Aspergillus pseudoglaucus MK82 ? - ? 89 3.4.23.18 additional information specificity Aspergillus niger B ? - ? 89 3.4.23.18 additional information no hydrolysis of benzyloxycarbonyl-Lys-Gly-Ala, benzyloxycarbonyl-Lys-(D)-Leu-Ala Aspergillus niger B ? - ? 89 3.4.23.18 Myoglobin + H2O - Aspergillus pseudoglaucus ? - ? 16359 3.4.23.18 Myoglobin + H2O - Aspergillus pseudoglaucus MK82 ? - ? 16359 3.4.23.18 N2-acetyl-D-arginyl-L-lysyl-L-isoleucyl-N-[(2S)-1-[(2S)-2-[[(1R)-4-carbamimidamido-1-carboxybutyl]carbamoyl]pyrrolidin-1-yl]-4-[methyl[(4-nitrophenoxy)carbonyl]amino]-1-oxobutan-2-yl]-L-argininamide + H2O - Aspergillus oryzae N2-acetyl-D-arginyl-L-lysyl-L-isoleucyl-L-arginine + 1-[(2S)-2-amino-4-[methyl[(4-nitrophenoxy)carbonyl]amino]butanoyl]-L-prolyl-D-argininamide - ir 450385 3.4.23.18 Oxidized insulin B-chain + H2O major cleavage sites: Phe24-Phe25, Leu15-Tyr16, minor sites: Ala14-Leu15, Tyr16-Leu17, peptide bond specificity compared to other proteinases Aspergillus phoenicis Hydrolyzed insulin B-chain - ? 15315 3.4.23.18 Proangiotensin + H2O cleavage sites: Tyr-Ile and His-Pro, Aspergillus oryzae enzyme, cleavage specificity compared to pepsin and cathepsin D Aspergillus oryzae ? - ? 16270 3.4.23.18 proangiotensin + H2O the enzyme cleaves the Xaa-pro bond with Xaa being any amino acid Aspergillus phoenicis DRVYIH + PFHLLVYS i.e. angiotensin I ? 383325 3.4.23.18 resorufin-labelled casein + H2O - Aspergillus fumigatus ? - ? 450551 3.4.23.18 resorufin-labelled casein + H2O - Aspergillus fumigatus A1160 ? - ? 450551 3.4.23.18 soy protein + H2O - Aspergillus pseudoglaucus ? - ? 450633 3.4.23.18 tert-Butoxycarbonyl-Ile-Glu-Gly-Arg 4-methylcoumarin 7-amide + H2O - Aspergillus phoenicis tert-Butoxycarbonyl-Ile-Glu-Gly + Arg 4-methylcoumarin 7-amide - ? 16276 3.4.23.18 tert-Butoxycarbonyl-Leu-Ser-Thr-Arg 4-methylcoumarin 7-amide + H2O - Aspergillus phoenicis tert-Butoxycarbonyl-Leu-Ser-Thr + Arg 4-methylcoumarin 7-amide - ? 16275 3.4.23.18 Trypsinogen + H2O - Aspergillus niger ? - ? 16266 3.4.23.18 Trypsinogen + H2O - Aspergillus oryzae ? - ? 16266 3.4.23.18 Trypsinogen + H2O - Aspergillus phoenicis ? - ? 16266 3.4.23.18 Trypsinogen + H2O cleaves Lys-Ile and liberates hexapeptide Aspergillus phoenicis ? - ? 16266 3.4.23.18 Trypsinogen + H2O bovine (pancreatic) Aspergillus oryzae ? - ? 16266 3.4.23.18 Trypsinogen + H2O better substrate than chymotrypsinogen Aspergillus oryzae ? - ? 16266 3.4.23.18 Trypsinogen + H2O bovine (trypsinogen) Aspergillus oryzae ? - ? 16266 3.4.23.18 Trypsinogen + H2O cleavage site: Lys6-Ile7 Aspergillus phoenicis ? - ? 16266 3.4.23.18 Trypsinogen + H2O bovine (pancreatic) Aspergillus oryzae 365-U-64-1 ? - ? 16266 3.4.23.18 Trypsinogen + H2O bovine (trypsinogen) Aspergillus oryzae 365-U-64-1 ? - ? 16266 3.4.23.18 Trypsinogen + H2O cleaves Lys-Ile and liberates hexapeptide Aspergillus phoenicis R-3813 ? - ? 16266 3.4.23.18 Trypsinogen + H2O - Aspergillus niger B ? - ? 16266 3.4.23.18 trypsinogen + H2O activation by cleavage of a Lys-Pro bond Aspergillus phoenicis trypsin + peptide fragment - ? 383394