3.4.22.71	2 amylase-binding adhesin AbpA	-	Streptococcus parasanguinis	(amylase-binding adhesin AbpA)2 + H2O	-	?	449169	
3.4.22.71	2 amylase-binding adhesin AbpA	-	Streptococcus parasanguinis FW213	(amylase-binding adhesin AbpA)2 + H2O	-	?	449169	
3.4.22.71	AbpA-LPKTSAVKLE-green fluorescent protein + H2O	C-terminal sequence of AbpA fused to green fluorescent protein	Streptococcus parasanguinis	AbpA-LPKT + SAVKLE-green fluorescent protein	-	?	449418	
3.4.22.71	AbpA-LPKTSAVKLE-green fluorescent protein + H2O	C-terminal sequence of AbpA fused to green fluorescent protein	Streptococcus parasanguinis FW213	AbpA-LPKT + SAVKLE-green fluorescent protein	-	?	449418	
3.4.22.71	Abz-KDFEVPTGVAM-Dap(Dnp)-NH2 + H2O	-	Streptococcus pyogenes	?	-	?	418372	
3.4.22.71	Abz-KDFEVPTGVAM-Dap(Dnp)-NH2 + H2O	-	Streptococcus pyogenes M1 SF370	?	-	?	418372	
3.4.22.71	Dabcyl-QANPQTNEE-Edans + H2O	-	Staphylococcus aureus	Dabcyl-QANPQT + NEE-Edans	-	?	449836	
3.4.22.71	Dabcyl-QANPQTNEE-Edans + H2O	-	Staphylococcus aureus ATCC 29213	Dabcyl-QANPQT + NEE-Edans	-	?	449836	
3.4.22.71	DNPNTGDE + H2O	-	Bacillus anthracis	?	-	?	449879	
3.4.22.71	IsdC + H2O	the enzyme cleaves the C-terminal sorting signal of IsdC at the NPQTN motif and rethers the polypeptide to the pentaglycine cell wall cross-bridge. During catalysis, the active site cysteine of sortase and the cleaved substrate form an acyl intermediate, which is then resolved by the amino group of pentaglycine cross-bridges	Staphylococcus aureus	?	-	?	362308	
3.4.22.71	IsdC + H2O	the enzyme anchors the IsdC precursor with a C-terminal NPQTN motif sorting sognal, to the cell wall envelope. The sorting signal of IsdC is cleaved between threonine and asparagine of the NPQTN motif, and the carboxyl group of the thrteonine is amide-linked to the amino group of pentaglycine cross-bridges	Staphylococcus aureus	?	-	?	362308	
3.4.22.71	IsdC + H2O	IsdC is a surface protein necessary for heme iron uptake in Bacillus anthracis. SrtB recognizes the NPKTG sequence present	Bacillus anthracis	?	-	?	362308	
3.4.22.71	Lmo2185 + H2O	-	Listeria monocytogenes	?	-	?	401326	
3.4.22.71	Lmo2186 + H2O	NPKSS is a sorting motif of Lmo2186. Recognition of NPKSS by SrtB, even when placed in the context of the heterologous sorting signal of Lmo2185. Proline at position 2, and not lysine at position 3, is essential for the recognition of NPKSS by SrtB	Listeria monocytogenes	?	-	?	401327	
3.4.22.71	additional information	gram-positive pathogenic bacteria display proteins on their surface that play important roles during infection. In Staphylococcus aureus these surface proteins are anchored to the cell wall by two sortases, sortase A and sortaseB that recognize specific surface protein sorting signals. Sortase B plays a contributing role during the pathogenesis of staphylococcal infections	Staphylococcus aureus	?	-	?	89	
3.4.22.71	additional information	anchoring of SvpA to the bacterial cell wall is specifically mediated by SrzB. The enzyme is involved in the attachment of a subset of proteins to the cell wall, most likely by recognizing an NXZTN sorting motif. SrtB-mediated anchoring can be required to anchor surface proteins involved in the adaption of this microorganism to different environmental conditions	Listeria monocytogenes	?	-	?	89	
3.4.22.71	additional information	the svpA-srtB locus is regulated by iron availability, mediated by Fur	Listeria monocytogenes	?	-	?	89	
3.4.22.71	additional information	non-gel proteomics is a powerful technique to rapidly identify sortase substrates and to gain insights on potential sorting motifs. Two surface proteins, Lmo2185 and Lmo2186 are identified only when SrtB is active. The analysis of the peptides identified in these proteins suggests that SrtB of Listeria monocytogenes may recognize two different sorting motifs, NXZTN and NPKXZ	Listeria monocytogenes	?	-	?	89	
3.4.22.71	additional information	sortase B may be critical in the early stage of inhaltation anthrax	Bacillus anthracis	?	-	?	89	
3.4.22.71	additional information	surface protein IsdC and sortase B are required for heme-iron scavenging of Bacillus anthracis	Bacillus anthracis	?	-	?	89	
3.4.22.71	PPKTG + H2O	-	Clostridioides difficile	?	-	?	450486	
3.4.22.71	PPKTG + H2O	-	Clostridioides difficile 630	?	-	?	450486	
3.4.22.71	SvpA + H2O	anchoring of SvpA to the bacterial cell wall is specifically mediated by SrzB	Listeria monocytogenes	?	-	?	362307