3.4.22.69 (Ala-Arg-Leu-Gln-NH)2-rhodamine - Severe acute respiratory syndrome-related coronavirus rhodamine 110 + (Ala-Arg-Leu-Gln-NH)-rhodamine - ? 383461 3.4.22.69 (CAL fluor red 610)-TSAVLQSGFRK(BHQ1) + H2O - Severe acute respiratory syndrome-related coronavirus (CAL fluor red 610)-TSAVLQ + SGFRK(BHQ1) - ? 390422 3.4.22.69 2-aminobenzoyl-SVTLQSG-Tyr(NO2)Arg + H2O - Severe acute respiratory syndrome-related coronavirus ? - ? 399404 3.4.22.69 2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide + H2O - Severe acute respiratory syndrome-related coronavirus 2-aminobenzoyl-TSAVLQ + SGFRK-2,4-dinitrophenyl amide - ? 399405 3.4.22.69 AAVLQSGF-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus AAVLQ + SGF-NH2 - ? 391104 3.4.22.69 Abz-LQSGFRK(Dnp)NH2 + H2O - Severe acute respiratory syndrome-related coronavirus Abz-LQ + SGFRK(Dnp)NH2 - ? 418381 3.4.22.69 Abz-LYQPPQTSITSAVLQSGFRK(Dnp)NH2 + H2O - Severe acute respiratory syndrome-related coronavirus Abz-LYQPPQTSITSAVLQ + SGFRK(Dnp)NH2 - ? 418382 3.4.22.69 Abz-QTSITSAVLQSGFRK(Dnp)NH2 + H2O - Severe acute respiratory syndrome-related coronavirus Abz-QTSITSAVLQ + SGFRK(Dnp)NH2 - ? 418391 3.4.22.69 Abz-SAVLQSGFRK(Dnp)NH2 + H2O - Severe acute respiratory syndrome-related coronavirus Abz-SAVLQ + SGFRK(Dnp)NH2 - ? 418399 3.4.22.69 Abz-SAVLQSGFRKMAFPSGK(Dnp)NH2 + H2O - Severe acute respiratory syndrome-related coronavirus Abz-SAVLQ + SGFRKMAFPSGK(Dnp)NH2 - ? 418402 3.4.22.69 Abz-SAVLQSGFRKMAK(Dnp)NH2 + H2O - Severe acute respiratory syndrome-related coronavirus Abz-SAVLQ + SGFRKMAK(Dnp)NH2 - ? 418400 3.4.22.69 Abz-SAVLQSGK(Dnp)NH2 + H2O - Severe acute respiratory syndrome-related coronavirus Abz-SAVLQ + SGK(Dnp)NH2 - ? 418401 3.4.22.69 Abz-SGADVLYQPPQTSITSAVLQSGFRK(Dnp)NH2 + H2O - Severe acute respiratory syndrome-related coronavirus Abz-SGADVLYQPPQTSITSAVLQ + SGFRK(Dnp)NH2 - ? 418403 3.4.22.69 Abz-VLQSGFRK(Dnp)NH2 + H2O - Severe acute respiratory syndrome-related coronavirus Abz-VLQ + SGFRK(Dnp)NH2 - ? 418414 3.4.22.69 acetyl-TSAVLH-7-amido-4-carbamoyl-coumarin + H2O SARS-CoV 3Clpro prefers Gln over His in P1 position. Unlike SARS-CoV 3Clpro, His is strongly preferred in the P1 position by 3C-like proteases from infectious bronchitis virus murine hepatitis virus Severe acute respiratory syndrome-related coronavirus acetyl-TSAVLH + 7-amino-4-carbamoyl-coumarin - ? 399868 3.4.22.69 acetyl-TSTKLQ-7-amido-4-carbamoyl-coumarin + H2O optimized fluorogenic peptide substrate. The enzyme exhibits a strong preference for P1 Gln containing substrates and P2 Leu containing substrates Severe acute respiratory syndrome-related coronavirus acetyl-TSTKLQ + 7-amino-4-carbamoyl-coumarin - ? 399869 3.4.22.69 ATVRLQAGNAT + H2O - Severe acute respiratory syndrome-related coronavirus ATVRLQ + AGNAT - ? 391388 3.4.22.69 AVLQS-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus AVLQ + L-serinamide - ? 391393 3.4.22.69 AVLQSE-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus AVLQ + Ser-Glu-NH2 - ? 391394 3.4.22.69 AVLQSGF-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus AVLQ + SGF-NH2 - ? 391395 3.4.22.69 coronavirus polyprotein + H2O 3CLpro processes the translated polyproteins to functional viral proteins Severe acute respiratory syndrome-related coronavirus ? - ? 398842 3.4.22.69 coronavirus polyprotein + H2O during the formation of the coronaviral replication/transcription complex, essential steps include processing of the conserved polyprotein nsp7-10 region by the main protease Mpro and subsequent complex formation of the released nsps Severe acute respiratory syndrome-related coronavirus ? - ? 398842 3.4.22.69 coronavirus polyprotein + H2O during the formation of the coronaviral replication/transcription complex, essential steps include processing of the conserved polyprotein nsp7-10 region by the main protease Mpro and subsequent complex formation of the released nsps. There is a clear order of cleavage efficiencies, which is influenced by the polyprotein tertiary structure. The predominant product is an nsp7+8(2:2) hetero-tetramer with nsp8 scaffold Severe acute respiratory syndrome-related coronavirus ? - ? 398842 3.4.22.69 cyan fluorescent protein-TSAVLQSGFRKM-yellow fluorescent protein + H2O - Severe acute respiratory syndrome-related coronavirus cyan fluorescent protein-TSAVLQ + SGFRKM-yellow fluorescent protein - ? 418556 3.4.22.69 Dabcyl-KNSTLQSGLRKE-EDANS + H2O - Severe acute respiratory syndrome-related coronavirus Dabcyl-KNSTLQ + SGLRKE-EDANS - ? 400801 3.4.22.69 Dabcyl-KTSAVLQSGFRKME-EDANS + H2O - Severe acute respiratory syndrome-related coronavirus Dabcyl-KTSAVLQ + SGFRKME-EDANS - ? 400802 3.4.22.69 Dabcyl-KTSAVLQSGFRKME-EDANS + H2O - feline coronavirus Dabcyl-KTSAVLQ + SGFRKME-EDANS - ? 400802 3.4.22.69 Dabcyl-KTSAVLQSGFRKME-EDANS + H2O - Severe acute respiratory syndrome coronavirus 2 Dabcyl-KTSAVLQ + SGFRKME-EDANS - ? 400802 3.4.22.69 Dabcyl-KTSAVLQSGFRKME-EDANS + H2O - Severe acute respiratory syndrome-related coronavirus Tor2 Dabcyl-KTSAVLQ + SGFRKME-EDANS - ? 400802 3.4.22.69 dabcyl-KTSAVLQSGFRKME-EDANS + H2O - Severe acute respiratory syndrome-related coronavirus ? - ? 411961 3.4.22.69 Dabcyl-KTSAVLQSGFRKME-EDANS-amide + H2O - Severe acute respiratory syndrome-related coronavirus Dabcyl-KTSAVLQ + SGFRKME-EDANS-amide - ? 418571 3.4.22.69 Dabcyl-KTSAVLQSGFRKMQ-EDANS + H2O - Severe acute respiratory syndrome-related coronavirus Dabcyl-KTSAVLQ + SGFRKMQ-EDANS - ? 400803 3.4.22.69 DABCYL-Lys-Asn-Ser-Thr-Leu-Gln-Ser-Gly-Leu-Arg-Lys-Glu-EDANS + H2O - Severe acute respiratory syndrome-related coronavirus DABCYL-Lys-Asn-Ser-Thr-Leu-Gln + Ser-Gly-Leu-Arg-Lys-Glu-EDANS - ? 411963 3.4.22.69 DABCYL-Lys-Thr-Ser-Ala-Val-Leu-Gln-Ser-Gly-Phe-Arg-Lys-Met-Glu-EDANS + H2O - Severe acute respiratory syndrome-related coronavirus DABCYL-Lys-Thr-Ser-Ala-Val-Leu-Gln + Ser-Gly-Phe-Arg-Lys-Met-Glu-EDANS - ? 411964 3.4.22.69 DABCYL-TSAVLQSGFRKME-EDANS + H2O internally quenched fluorescent peptide containing a consensus cleavage sequence, with DABCYL at the N-terminal and EDANS at the C-terminal end Severe acute respiratory syndrome coronavirus 2 DABCYL-TSAVLQ + SGFRKME-EDANS - ? 462415 3.4.22.69 DABCYL-TSAVLQSGFRKME-EDANS + H2O internally quenched fluorescent peptide containing a consensus cleavage sequence, with DABCYL at the N-terminal and EDANS at the C-terminal end severe acute respiratory syndrome coronavirus DABCYL-TSAVLQ + SGFRKME-EDANS - ? 462415 3.4.22.69 EDANS-VNSTLQSGLRK-(Dabcyl)-M + H2O - Severe acute respiratory syndrome-related coronavirus EDANS-VNSTLQ + SGLRK-(Dabcyl)-M - ? 400906 3.4.22.69 FAVLQSGF + H2O - Severe acute respiratory syndrome-related coronavirus FAVLQ + SGF - ? 418620 3.4.22.69 FVRLQSGF + H2O - Severe acute respiratory syndrome-related coronavirus FVRLQ + SGF - ? 418639 3.4.22.69 FVVLQSGF + H2O - Severe acute respiratory syndrome-related coronavirus FVVLQ + SGF - ? 418640 3.4.22.69 FYPKLQASQAW + H2O - Severe acute respiratory syndrome-related coronavirus FYPKLQ + ASQAW - ? 392008 3.4.22.69 GPFVDRQTAQAAGTDT-NH2 + H2O 1% of the activity with TSAVLQSGFRK-NH2 Severe acute respiratory syndrome-related coronavirus ? - ? 401089 3.4.22.69 H-Thr-Ser-Ala-Val-Leu-Gln-Ser-Gly-Phe-Arg-Lys-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus H-Thr-Ser-Ala-Val-Leu-Gln + Ser-Gly-Phe-Arg-Lys-NH2 - ? 418706 3.4.22.69 H-Thr-Ser-Ala-Val-Leu-Gln-Ser-Gly-Phe-Arg-Lys-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus ? - ? 450024 3.4.22.69 KVATVQSKMSD + H2O - Severe acute respiratory syndrome-related coronavirus KVATVQ + SKMSD - ? 392339 3.4.22.69 KVATVQSKMSD + H2O weak activity Severe acute respiratory syndrome-related coronavirus KVATVQ + SKMSD - ? 392339 3.4.22.69 KVATVQSKMSD-NH2 undecapeptide containing the non-canonical P3/P4 cleavage site of 3CL protease, 6% of the activity with TSAVLQSGFRK-NH2 Severe acute respiratory syndrome-related coronavirus ? - ? 401238 3.4.22.69 L-Thr-L-Ser-L-Ala-L-Val-L-Leu-L-Gln-4-nitroanilide + H2O - Severe acute respiratory syndrome-related coronavirus L-Thr-L-Ser-L-Ala-L-Val-L-Leu-L-Gln + 4-nitroaniline - ? 412336 3.4.22.69 LAVLQSGF-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus LAVLQ + SGF-NH2 - ? 392471 3.4.22.69 LQSG-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus Leu-Gln + Ser-Gly-NH2 - ? 392497 3.4.22.69 MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 + H2O FRET peptide substrate Severe acute respiratory syndrome coronavirus 2 ? - ? 462685 3.4.22.69 MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 + H2O - Severe acute respiratory syndrome coronavirus 2 MCA-AVLQ + SGFR-Lys(Dnp)-Lys-NH2 - ? 462686 3.4.22.69 MCAAVLQSGFR-Lys(Dnp)-Lys-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus MCAAVLQ + Ser-Gly-Phe-Arg-Lys(Dnp)-Lys-NH2 - ? 401357 3.4.22.69 additional information a 3CLpro mechanism utilizes an electrostatic trigger to initiate the acylation reaction, a cysteine-histidine catalytic dyad ion pair, an enzyme-facilitated release of P1, and a general base catalyzed deacylation reaction Severe acute respiratory syndrome-related coronavirus ? - ? 89 3.4.22.69 additional information complete description of the tetrapeptide substrate specificity of 3Clpro using fully degenerate peptide libraries consisting of all 160 000 possible naturally occurring tetrapeptides. P1-Gln P2-Leu specificity and elucidate a novel preference for P1-His containing substrates equal to the expected preference for P1-Gln Severe acute respiratory syndrome-related coronavirus ? - ? 89 3.4.22.69 additional information SARS-CoV 3CLpro mediates extensive proteolytic processing of two overlapping replicase polyproteins, pp1a (486000 Da) and pp1ab (790000 Da), to yield the corresponding functional polypeptides that are essential for SARSCoV replication and transcription processes Severe acute respiratory syndrome-related coronavirus ? - ? 89 3.4.22.69 additional information the genomic RNA produces two large proteins with overlapping sequences, polyproteins 1a and 1ab, which are autocatalytically cleaved by two or three viral proteases to yield functional polypeptides. The key enzyme in this processing is SARS 3CL protease Severe acute respiratory syndrome-related coronavirus ? - ? 89 3.4.22.69 additional information a complete description of the tetrapeptide substrate specificity of 3Clpro using fully degenerate peptide libraries consisting of all 160000 possible naturally occurring tetrapeptides. The enzyme exhibits a strong preference for P1 Gln containing substrates and P2 Leu containing substrates. The enzyme also shows a strong preference for P1 histidine containing substrates. 3Clpro has extended substrate specificity at P5 and P6 preferring hydrophobic amino acids such as Leu Severe acute respiratory syndrome-related coronavirus ? - ? 89 3.4.22.69 additional information comprehensive overview of SARS-CoV 3CLpro substrate specificity. The hydrophobic pocket in the P2 position at the protease cleavage site is crucial to SARS-CoV 3CLpro-specific binding, which is limited to substitution by hydrophobic residue. The binding interface of SARS-CoV 3CLpro that is facing the P1' position is suggested to be occupied by acidic amino acids, thus the P1' position is intolerant to acidic residue substitution, owing to electrostatic repulsion. Steric hindrance caused by some bulky or branching amino acids in P3 and P2' positions may also hinder the binding of SARS-CoV 3CLpro. In addition to the conserved Gln residue in the P1 position at the SARS-CoV 3CLpro cleavage site, the P2 position, which is exclusively occupied by Leu residue, also serves as another important determinant of substrate specificity. Peptide substrate with Phe replacement in the P2 position is also favorable for SARSCoV 3CLpro cleavage. Ile is intolerant in the P2 position. P1' position, which is frequently occupied by Ser residue, also contributes to the substrate specificity of SARS-CoV 3CLpro considerably. The P1' position is highly unfavorable to the substitution by Pro, Asp, and Glu residues. The substrate specificity of SARS-CoV 3CLpro is less dependent on the P2' and P3 positions at the cleavage site. The peptide cleavage results show that the P3' and P4 positions have no effect on determining the substrate specificity preferences of SARS-CoV 3CLpro Severe acute respiratory syndrome-related coronavirus ? - ? 89 3.4.22.69 additional information cuts the 11 peptides covering all of the 11 cleavage sites on the viral polyprotein with different efficiency Severe acute respiratory syndrome-related coronavirus ? - ? 89 3.4.22.69 additional information the S3 subsite of the SARS CoVMpro has a negative character. The electrostatic interactions between Glu47 and P3Lys play a key role in specific binding. These observations are very important and provide further information for structural-based drug design against SARS virus Severe acute respiratory syndrome-related coronavirus ? - ? 89 3.4.22.69 additional information 3CLpro cleaves the replicase polyproteins at 11 sites with the conserved Gln-(Ser, Ala, Gly) sequences Severe acute respiratory syndrome-related coronavirus ? - ? 89 3.4.22.69 additional information no cleavage of SAVLQPGFRK Severe acute respiratory syndrome-related coronavirus ? - ? 89 3.4.22.69 additional information no activity towards SWTSAVKQSGFRKWA, SWTSAVRQSGFRKWA, SWTSAVEQSGFRKWA, and SWTSAVDQSGFRKWA Severe acute respiratory syndrome-related coronavirus ? - ? 89 3.4.22.69 additional information the substrate specificity of the enzyme requires glutamine in the P1 position and a large hydrophobic residue in the P2 position Severe acute respiratory syndrome-related coronavirus ? - ? 89 3.4.22.69 additional information the enzyme performs autoprocessing, overview. As 3CLpro requires a glutamine residue at the P1 position of the substrate Severe acute respiratory syndrome-related coronavirus ? - ? 89 3.4.22.69 additional information the enzyme shows a substrate-induced catalytic mechanism that further enhances its substrate specificity, overview Severe acute respiratory syndrome-related coronavirus ? - ? 89 3.4.22.69 additional information SARSCoV 3CLpro favors Ala and Ser, followed by Gly at the P1' position severe acute respiratory syndrome coronavirus ? - - 89 3.4.22.69 additional information the N-terminal cleavage site G-S of substrate is preferred over the C-terminal cleavage site Q-G Severe acute respiratory syndrome coronavirus 2 ? - - 89 3.4.22.69 N-methylanthranilyl-TSAVLQSGFRK(2,4-dinitrophenyl) + H2O - Severe acute respiratory syndrome-related coronavirus ? - ? 450367 3.4.22.69 NRATLQAIASE + H2O - Severe acute respiratory syndrome-related coronavirus NRATLQ + AIASE - ? 392898 3.4.22.69 NRATLQAIASE + H2O weak activity Severe acute respiratory syndrome-related coronavirus NRATLQ + AIASE - ? 392898 3.4.22.69 NVATLQAENVT + H2O - Severe acute respiratory syndrome-related coronavirus NVATLQ + AENVT - ? 392902 3.4.22.69 NVATLQAENVT + H2O weak activity Severe acute respiratory syndrome-related coronavirus NVATLQ + AENVT - ? 392902 3.4.22.69 o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide + H2O - Severe acute respiratory syndrome-related coronavirus o-aminobenzoyl-TSAVLQSGFRK + 2,4-dinitroaniline - ? 453108 3.4.22.69 o-aminobenzoyl-TSAVLQSGFRY(3-NO2)G + H2O - Severe acute respiratory syndrome-related coronavirus o-aminobenzoyl-TSAVLQ + SGFRY(3-NO2)G - ? 393014 3.4.22.69 PATVLQAVGAC + H2O - Severe acute respiratory syndrome-related coronavirus PATVLQ + AVGAC - ? 393172 3.4.22.69 PHTVLQAVGAC + H2O - Severe acute respiratory syndrome-related coronavirus PHTVLQ + AVGAC - ? 401753 3.4.22.69 pp1a + H2O cleavage of a viron polyprotein Severe acute respiratory syndrome-related coronavirus ? - ? 432309 3.4.22.69 pp1ab + H2O cleavage of a viron polyprotein Severe acute respiratory syndrome-related coronavirus ? - ? 432310 3.4.22.69 REPLMQSADAS + H2O - Severe acute respiratory syndrome-related coronavirus REPLMQ + SADAS - ? 393416 3.4.22.69 REPLMQSADAS + H2O weak activity Severe acute respiratory syndrome-related coronavirus REPLMQ + SADAS - ? 393416 3.4.22.69 SAALQSGF-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus SAALQ + SGF-NH2 - ? 393456 3.4.22.69 SAKLQSGF-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus SAKLQ + SGF-NH2 - ? 393458 3.4.22.69 SALLQSGF-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus SALLQ + SGF-NH2 - ? 393462 3.4.22.69 SARS-CoV Trx/GST-tagged C145A 3CLpro mutant + 2 H2O contains 2 x 10 additional amino acids for cleavage, substrate for 3CLpro processing by the wild-type 3CLpro, substrate is catalytically inactive Severe acute respiratory syndrome coronavirus 2 Trx-10aa + C145A 3CLpro mutant + 10aa-GST - ? 463020 3.4.22.69 SARS-CoV Trx/GST-tagged C145A 3CLpro mutant + 2 H2O contains 2 x 10 additional amino acids for cleavage, substrate for 3CLpro processing by the wild-type 3CLpro, substrate is catalytically inactive severe acute respiratory syndrome coronavirus Trx-10aa + C145A 3CLpro mutant + 10aa-GST - ? 463020 3.4.22.69 SARS-CoV-2 Trx/GST-tagged C145A 3CLpro mutant + 2 H2O contains 2 x 10 additional amino acids for cleavage, substrate for 3CLpro processing by the wild-type 3CLpro, substrate is catalytically inactive Severe acute respiratory syndrome coronavirus 2 Trx-10aa + C145A 3CLpro mutant + 10aa-GST - ? 463021 3.4.22.69 SARS-CoV-2 Trx/GST-tagged C145A 3CLpro mutant + 2 H2O contains 2 x 10 additional amino acids for cleavage, substrate for 3CLpro processing by the wild-type 3CLpro, substrate is catalytically inactive severe acute respiratory syndrome coronavirus Trx-10aa + C145A 3CLpro mutant + 10aa-GST - ? 463021 3.4.22.69 SATLQSGF-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus SATLQ + SGF-NH2 - ? 393468 3.4.22.69 SAVAQSGF-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus SAVAQ + SGF-NH2 - ? 393469 3.4.22.69 SAVFQSGF-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus SAVMQ + SGF-NH2 - ? 393470 3.4.22.69 SAVIQSGF-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus SAVIQ + SGF-NH2 - ? 393471 3.4.22.69 SAVKLQNNELS + H2O - Severe acute respiratory syndrome-related coronavirus SAVKLQ + NNELS - ? 393472 3.4.22.69 SAVKLQNNELS + H2O weak activity Severe acute respiratory syndrome-related coronavirus SAVKLQ + NNELS - ? 393472 3.4.22.69 SAVLESGF-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus SAVLE + SGF-NH2 - ? 393473 3.4.22.69 SAVLKSGF-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus SAVLK + SGF-NH2 - ? 393474 3.4.22.69 SAVLNSGF-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus SAVLN + SGF-NH - ? 393475 3.4.22.69 SAVLQAGF-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus SAVLQ + AGF-NH2 - ? 393476 3.4.22.69 SAVLQEGFRK + H2O the cleavage rate of the mutant enzyme T25G is remarkably higher compared to the wild type enzyme Severe acute respiratory syndrome-related coronavirus SAVLQ + EGFRK - ? 412854 3.4.22.69 SAVLQFGFRK + H2O the cleavage rate of the mutant enzyme T25G is remarkably higher compared to the wild type enzyme Severe acute respiratory syndrome-related coronavirus SAVLQ + FGFRK - ? 412855 3.4.22.69 SAVLQGGF-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus SAVLQ + GGF-NH2 - ? 393477 3.4.22.69 SAVLQGGFRK + H2O the cleavage rate of the mutant enzyme T25G is similar to the wild type enzyme Severe acute respiratory syndrome-related coronavirus SAVLQ + GGFRK - ? 412856 3.4.22.69 SAVLQHGFRK + H2O low activity Severe acute respiratory syndrome-related coronavirus SAVLQ + HGFRK - ? 412857 3.4.22.69 SAVLQKGFRK + H2O low activity Severe acute respiratory syndrome-related coronavirus SAVLQ + KGFRK - ? 412858 3.4.22.69 SAVLQLGF-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus SAVLQ + LGF-NH2 - ? 393478 3.4.22.69 SAVLQLGFRK + H2O the cleavage rate of the mutant enzyme T25G is remarkably higher compared to the wild type enzyme Severe acute respiratory syndrome-related coronavirus SAVLQ + LGFRK - ? 412859 3.4.22.69 SAVLQMGFRK + H2O the cleavage rate of the mutant enzyme T25G is remarkably higher compared to the wild type enzyme Severe acute respiratory syndrome-related coronavirus SAVLQ + MGFRK - ? 412860 3.4.22.69 SAVLQSGF-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus SAVLQ + SGF-NH2 - ? 393479 3.4.22.69 SAVLQSGFRK + H2O - Severe acute respiratory syndrome-related coronavirus SAVLQ + SGFRK - ? 412861 3.4.22.69 SAVLQSGFRK + H2O best substrate for both, wild type and mutant enzyme T25G Severe acute respiratory syndrome-related coronavirus SAVLQ + SGFRK - ? 412861 3.4.22.69 SAVLQWGFRK + H2O low activity Severe acute respiratory syndrome-related coronavirus SAVLQ + WGFRK - ? 412862 3.4.22.69 SAVMQSGF-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus SAVMQ + SGF-NH2 - ? 393480 3.4.22.69 SAVRQSGF-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus SAVRQ + SGF-NH2 - ? 393481 3.4.22.69 SAVVQSGF-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus SAVVQ + SGF-NH2 - ? 393482 3.4.22.69 Ser-Ala-Val-Leu-Gln-Leu-Gly-Phe-Arg-Lys + H2O substrate for T25G mutant protein Severe acute respiratory syndrome-related coronavirus Ser-Ala-Val-Leu-Gln + Leu-Gly-Phe-Arg-Lys - ? 412869 3.4.22.69 Ser-Ala-Val-Leu-Gln-Met-Gly-Phe-Arg-Lys + H2O - Severe acute respiratory syndrome-related coronavirus Ser-Ala-Val-Leu-Gln + Met-Gly-Phe-Arg-Lys - ? 412870 3.4.22.69 Ser-Ala-Val-Leu-Gln-Ser-Gly-Phe-Arg-Lys + H2O - Severe acute respiratory syndrome-related coronavirus Ser-Ala-Val-Leu-Gln + Ser-Gly-Phe-Arg-Lys - ? 412871 3.4.22.69 SGVTFQGKFKK + H2O - Severe acute respiratory syndrome-related coronavirus SGVTFQ + GKFKK - ? 393492 3.4.22.69 SGVTFQGKFKK + H2O highest cleavage efficiency. The two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive ones Severe acute respiratory syndrome-related coronavirus SGVTFQ + GKFKK - ? 393492 3.4.22.69 SITSAVLQ-p-nitroanilide + H2O - Severe acute respiratory syndrome-related coronavirus ? - ? 393500 3.4.22.69 SITSAVLQ-p-nitrophenyl ester + H2O - Severe acute respiratory syndrome-related coronavirus ? - ? 393501 3.4.22.69 SITSAVLQSGFRKMA + H2O - Severe acute respiratory syndrome-related coronavirus ? - ? 393502 3.4.22.69 SLVLQSGF-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus SLVLQ + SGF-NH2 - ? 393504 3.4.22.69 STVLQSGF-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus STVLQ + SGF-NH2 - ? 393551 3.4.22.69 SVVLQSGF-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus SVVLQ + SGF-NH2 - ? 393619 3.4.22.69 SWTSAVAQSGFRKWA + H2O less than 10% activity compared to SWTSAVLQSGFRKWA Severe acute respiratory syndrome-related coronavirus SWTSAVAQ + SGFRKWA - ? 419106 3.4.22.69 SWTSAVCQSGFRKWA + H2O less than 1% activity compared to SWTSAVLQSGFRKWA Severe acute respiratory syndrome-related coronavirus SWTSAVCQ + SGFRKWA - ? 419107 3.4.22.69 SWTSAVFQSGFRKWA + H2O about 70% activity compared to SWTSAVLQSGFRKWA Severe acute respiratory syndrome-related coronavirus SWTSAVFQ + SGFRKWA - ? 419108 3.4.22.69 SWTSAVHQSGFRKWA + H2O less than 5% activity compared to SWTSAVLQSGFRKWA Severe acute respiratory syndrome-related coronavirus SWTSAVHQ + SGFRKWA - ? 419109 3.4.22.69 SWTSAVIQSGFRKWA + H2O about 45% activity compared to SWTSAVLQSGFRKWA Severe acute respiratory syndrome-related coronavirus SWTSAVIQ + SGFRKWA - ? 419110 3.4.22.69 SWTSAVLQSGFRKWA + H2O - Severe acute respiratory syndrome-related coronavirus ? - ? 383523 3.4.22.69 SWTSAVLQSGFRKWA + H2O 100% activity Severe acute respiratory syndrome-related coronavirus SWTSAVLQ + SGFRKWA - ? 419111 3.4.22.69 SWTSAVMQSGFRKWA + H2O about 58% activity compared to SWTSAVLQSGFRKWA Severe acute respiratory syndrome-related coronavirus SWTSAVMQ + SGFRKWA - ? 419112 3.4.22.69 SWTSAVNQSGFRKWA + H2O less than 1% activity compared to SWTSAVLQSGFRKWA Severe acute respiratory syndrome-related coronavirus SWTSAVNQ + SGFRKWA - ? 419113 3.4.22.69 SWTSAVPQSGFRKWA + H2O about 10% activity compared to SWTSAVLQSGFRKWA Severe acute respiratory syndrome-related coronavirus SWTSAVPQ + SGFRKWA - ? 419114 3.4.22.69 SWTSAVQQSGFRKWA + H2O less than 5% activity compared to SWTSAVLQSGFRKWA Severe acute respiratory syndrome-related coronavirus SWTSAVQQ + SGFRKWA - ? 419115 3.4.22.69 SWTSAVSQSGFRKWA + H2O less than 1% activity compared to SWTSAVLQSGFRKWA Severe acute respiratory syndrome-related coronavirus SWTSAVSQ + SGFRKWA - ? 419116 3.4.22.69 SWTSAVTQSGFRKWA + H2O less than 5% activity compared to SWTSAVLQSGFRKWA Severe acute respiratory syndrome-related coronavirus SWTSAVTQ + SGFRKWA - ? 419117 3.4.22.69 SWTSAVVQSGFRKWA + H2O about 55% activity compared to SWTSAVLQSGFRKWA Severe acute respiratory syndrome-related coronavirus SWTSAVVQ + SGFRKWA - ? 419118 3.4.22.69 SWTSAVWQSGFRKWA + H2O less than 5% activity compared to SWTSAVLQSGFRKWA Severe acute respiratory syndrome-related coronavirus SWTSAVWQ + SGFRKWA - ? 419119 3.4.22.69 SWTSAVYQSGFRKWA + H2O less than 5% activity compared to SWTSAVLQSGFRKWA Severe acute respiratory syndrome-related coronavirus SWTSAVYQ + SGFRKWA - ? 419120 3.4.22.69 TAVLQSGF + H2O lowest activity Severe acute respiratory syndrome-related coronavirus TAVLQ + SGF - ? 419129 3.4.22.69 TAVLQSGF-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus TAVLQ + SGF-NH2 - ? 393641 3.4.22.69 TFTRLQSLENV + H2O - Severe acute respiratory syndrome-related coronavirus TFTRLQ + SLENV - ? 393657 3.4.22.69 Thr-Ser-Ala-Val-Leu-Gln-4-nitroanilide + H2O - Severe acute respiratory syndrome-related coronavirus Thr-Ser-Ala-Val-Leu-Gln + 4-nitroaniline - ? 450763 3.4.22.69 Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide + H2O - Severe acute respiratory syndrome-related coronavirus Thr-Ser-Ala-Val-Leu-Gln + p-nitroaniline - ? 412978 3.4.22.69 Thr-Ser-Ala-Val-Leu-Gln-Ser-Gly-Phe-Arg-Lys + H2O - Severe acute respiratory syndrome-related coronavirus Thr-Ser-Ala-Val-Leu-Gln + Ser-Gly-Phe-Arg-Lys - ? 450764 3.4.22.69 Thr-Ser-Ala-Val-Leu-Gln-Ser-Gly-Phe-Arg-Lys-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus Thr-Ser-Ala-Val-Leu-Gln + Ser-Gly-Phe-Arg-Lys-NH2 - ? 432438 3.4.22.69 TSAVLQ-4-nitroanilide + H2O - Severe acute respiratory syndrome-related coronavirus TSAVLQ + 4-nitroaniline - ? 432447 3.4.22.69 TSAVLQSGFRK-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus TSAVLQ + SGFRK-NH2 - ? 393743 3.4.22.69 TSAVLQSGFRK-NH2 + H2O highest cleavage efficiency. The two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive ones Severe acute respiratory syndrome-related coronavirus TSAVLQ + SGFRK-NH2 - ? 393743 3.4.22.69 TSAVLQSGFRK-NH2 + H2O peptide containing the P1/P2 cleavage site, the N-terminal self-cleavage site of the protease, most suitable substrate Severe acute respiratory syndrome-related coronavirus TSAVLQ + SGFRK-NH2 - ? 393743 3.4.22.69 TVILQAGF + H2O - Severe acute respiratory syndrome-related coronavirus TVILQ + Ala-Gly-Phe - ? 402074 3.4.22.69 TVKLQAGF + H2O - Severe acute respiratory syndrome-related coronavirus TVKLQ + Ala-Gly-Phe - ? 402075 3.4.22.69 TVKLQAGF-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus TVKLQ + AGF-NH2 - ? 393753 3.4.22.69 TVRLQAGF + H2O - Severe acute respiratory syndrome-related coronavirus TVRLQ + Ala-Gly-Phe - ? 402076 3.4.22.69 TVRLQSGF + H2O highest activity Severe acute respiratory syndrome-related coronavirus TVRLQ + SGF - ? 419156 3.4.22.69 TVTLQSGF-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus TVTLQ + SGF-NH2 - ? 393754 3.4.22.69 TVVLQSGF + H2O - Severe acute respiratory syndrome-related coronavirus TVVLQ + SGF - ? 419157 3.4.22.69 TVVLQSGF-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus TVVLQ + SGF-NH2 - ? 393755 3.4.22.69 VAVLQSGF + H2O - Severe acute respiratory syndrome-related coronavirus VAVLQ + SGF - ? 419184 3.4.22.69 VLQS-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus VLQ + L-serinamide - ? 393828 3.4.22.69 VLQSG-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus VLQ + Ser-Gly-NH2 - ? 393829 3.4.22.69 VVRLQSGF + H2O - Severe acute respiratory syndrome-related coronavirus VVRLQ + SGF - ? 419195 3.4.22.69 VVTLQSGF-NH2 + H2O - Severe acute respiratory syndrome-related coronavirus VVTLQ + SGF-NH2 - ? 393837 3.4.22.69 VVVLQSGF + H2O - Severe acute respiratory syndrome-related coronavirus VVVLQ + SGF - ? 419196 3.4.22.69 [4-(4-dimethylaminophenylazo)benzoic acid]-KNSTLQSGLRKE-[5-[2'-(aminoethyl)amino]-naphthalenesulfonic acid] + H2O - Severe acute respiratory syndrome-related coronavirus ? - ? 393886 3.4.22.69 [4-(4-dimethylaminophenylazo)benzoic acid]-KTSAVLQSGF RKME-[5-[2'-(aminoethyl)amino]-naphthalenesulfonic acid] + H2O - Severe acute respiratory syndrome-related coronavirus ? - ? 393887 3.4.22.69 [4-(4-dimethylaminophenylazo)benzoic acid]-KTSAVLQSGFRKME-5-['-(aminoethyl)amino]-naphthalenesulfonic acid + H2O - Severe acute respiratory syndrome-related coronavirus ? - ? 453109 3.4.22.69 [4-(4-dimethylaminophenylazo)benzoic acid]-KTSAVLQSGFRKME-5-[2'-(aminoethyl)amino]-naphthalenesulfonic acid + H2O - Severe acute respiratory syndrome-related coronavirus [4-(4-dimethylaminophenylazo)benzoic acid]-KTSAVLQ + SGFRKME-5-[2'-(aminoethyl)amino]-naphthalenesulfonic acid - ? 454023 3.4.22.69 [4-(4-dimethylaminophenylazo)benzoic acid]-KTSAVLQSGFRKME-[5-[2'-(aminoethyl)amino]-naphthalenesulfonic acid] + H2O - Severe acute respiratory syndrome-related coronavirus ? - ? 393888 3.4.22.69 [4-(4-dimethylaminophenylazo)benzoic acid]-VNSTLQSGLRK-[5-[2'-(aminoethyl)amino]-naphthalenesulfonic acid]-M + H2O - Alphacoronavirus ? - ? 393890