3.4.22.49 acetyl-Asp-Arg-Glu-Ile-Nle-Arg-7-amido-4-methylcoumarin + H2O acetyl-Asp-Arg-Glu-Ile-Nle-Arg is the Rad21-peptidyl Homo sapiens acetyl-Asp-Arg-Glu-Ile-Nle-Arg + 7-amino-4-methylcoumarin - ? 411547 3.4.22.49 Cdc14 + H2O separase regulates INCENP-Aurora B anaphase spindle function through activation of Cdc14 Saccharomyces cerevisiae ? - ? 380570 3.4.22.49 cohesin + H2O - Homo sapiens fragments of cohesin - ? 355593 3.4.22.49 cohesin + H2O - Saccharomyces cerevisiae fragments of cohesin - ? 355593 3.4.22.49 cohesin + H2O - Arabidopsis thaliana fragments of cohesin - ? 355593 3.4.22.49 cohesin + H2O - Schizosaccharomyces pombe fragments of cohesin - ? 355593 3.4.22.49 cohesin + H2O separase Esp1 is implicated in the removal of cohesin which links sister chromatids in the S-phase and is postulated to be a specific protease or a positive regulator of protease against cohesin Saccharomyces cerevisiae fragments of cohesin - ? 355593 3.4.22.49 cohesin + H2O - Saccharomyces cerevisiae SK1 fragments of cohesin - ? 355593 3.4.22.49 cohesin + H2O - Homo sapiens cleaved cohesin - ? 370481 3.4.22.49 cohesin + H2O - Saccharomyces cerevisiae cleaved cohesin - ? 370481 3.4.22.49 cohesin + H2O - Arabidopsis thaliana cleaved cohesin - ? 370481 3.4.22.49 cohesin + H2O - Schizosaccharomyces pombe cleaved cohesin - ? 370481 3.4.22.49 cohesin + H2O separase is required for sister chromatid separation during mitosis Homo sapiens cleaved cohesin - ? 370481 3.4.22.49 cohesin + H2O separase, i.e. Esp1, is implicated in the removal of cohesin which links sister chromatids in the S-phase and is postulated to be a specific protease or a positive regulator of protease against cohesin Saccharomyces cerevisiae cleaved cohesin - ? 370481 3.4.22.49 cohesin + H2O separase triggers anaphase by cohesin cleavage, and triggers the release and activation of the phosphatase Cdc14 independently of its protease activity Saccharomyces cerevisiae cleaved cohesin - ? 370481 3.4.22.49 cohesin + H2O - Drosophila melanogaster ? - ? 380577 3.4.22.49 cohesin + H2O - Chlamydomonas reinhardtii ? - ? 380577 3.4.22.49 cohesin + H2O - Mus musculus ? - ? 380577 3.4.22.49 cohesin + H2O - Homo sapiens ? - ? 380577 3.4.22.49 cohesin + H2O - Saccharomyces cerevisiae ? - ? 380577 3.4.22.49 cohesin + H2O - Schizosaccharomyces pombe ? - ? 380577 3.4.22.49 cohesin + H2O - Caenorhabditis elegans ? - ? 380577 3.4.22.49 cohesin + H2O - Ricinus communis ? - ? 380577 3.4.22.49 cohesin + H2O - Sorghum bicolor ? - ? 380577 3.4.22.49 cohesin + H2O - Oryza sativa ? - ? 380577 3.4.22.49 cohesin + H2O - Vitis vinifera ? - ? 380577 3.4.22.49 cohesin + H2O - Arabidopsis thaliana ? - ? 380577 3.4.22.49 cohesin + H2O - Cryptosporidium muris ? - ? 380577 3.4.22.49 cohesin + H2O - Thermochaetoides thermophila ? - ? 380577 3.4.22.49 cohesin + H2O separase-mediated cleavage of cohesin at interphase is required for DNA repair. It is proposed that the securin-separase complex might aid DNA repair by removing local cohesin in interphase cells Schizosaccharomyces pombe ? - ? 380577 3.4.22.49 cohesin + H2O cohesin cleavage by human separase requires DNA in a sequence-nonspecific manner Homo sapiens ? - ? 380577 3.4.22.49 cohesin + H2O separase selectively cleaves only the chromosome-associated cohesin with the cofactor chromosomal DNA Mus musculus ? - ? 380577 3.4.22.49 cohesin + H2O separase selectively cleaves only the chromosome-associated cohesin with the cofactor chromosomal DNA Saccharomyces cerevisiae ? - ? 380577 3.4.22.49 cohesin + H2O separase selectively cleaves only the chromosome-associated cohesin with the cofactor chromosomal DNA, chromosome-associated cohesin is cleaved preferentially by separase, mechanism Homo sapiens ? - ? 380577 3.4.22.49 cohesin + H2O chromatin-bound cohesion is the preferential substrate Mus musculus ? - ? 380577 3.4.22.49 cohesin + H2O cohesin Is cleaved with similar kinetics and timing near the centromere and telomere. Separase inhibition of PP2ACdc55 is necessary for robust cleavage of cohesin Saccharomyces cerevisiae ? - ? 380577 3.4.22.49 cohesin + H2O the enzyme cleaves the kleisin subunit of cohesin and opens the cohesin ring to allow chromosome segregation Thermochaetoides thermophila ? - ? 380577 3.4.22.49 cohesin + H2O - Thermochaetoides thermophila DSM 1495 ? - ? 380577 3.4.22.49 cohesin + H2O the enzyme cleaves the kleisin subunit of cohesin and opens the cohesin ring to allow chromosome segregation Thermochaetoides thermophila DSM 1495 ? - ? 380577 3.4.22.49 CPAR-1 protein + H2O - Caenorhabditis elegans ? - ? 448527 3.4.22.49 CPAR-1 protein + H2O separase cleaves the N-tail of the CENP-A related protein CPAR-1 at the meiosis I metaphase-anaphase transition Caenorhabditis elegans ? - ? 448527 3.4.22.49 kendrin + H2O - Homo sapiens ? - ? 431042 3.4.22.49 kendrin + H2O kendrin, also named pericentrin and specifically cleaved at a consensus site (R2231), is a crucial substrate for separase at the centrosome, protecting the engaged centrioles from premature disengagement and thereby blocking reduplication until the cell passes through mitosis Homo sapiens ? - ? 431042 3.4.22.49 additional information auto-cleavage of separase coordinates multiple aspects of the G2/M programme in human cells, thus contributing to the timing and efficiency of chromosome segregation Homo sapiens ? - ? 89 3.4.22.49 additional information deletion of separase specifically blocks sister chromatid separation but not other aspects of mitosis, mitotic exit, cytokinesis, or even chromosome replication Mus musculus ? - ? 89 3.4.22.49 additional information downregulation of PP2A(Cdc55) phosphatase by separase initiates mitotic exit. At anaphase onset, PP2A(Cdc55) activity is downregulated in a separase-dependent manner, triggering a first wave of Cdk-dependent Net1 phosphorylation and Cdc14 release. Separase is the essential trigger for Cdc14 activation in anaphase Saccharomyces cerevisiae ? - ? 89 3.4.22.49 additional information Hela cells lacking separase are delayed or arrested at the G2-M phase transition. Without separase, cells also have a prolonged prometaphase. Separase is required at multiple cell cycle stages including the interphase. Its role in promoting loss of sister chromatid cohesion might be important preferentially at arms but not centromers Homo sapiens ? - ? 89 3.4.22.49 additional information MAP kinase is required for the increased securin-separase interaction that rescues separase mutants under stresses Schizosaccharomyces pombe ? - ? 89 3.4.22.49 additional information separase auto-cleavage coordinates multiple aspects of the G2/M programme in human cells, thus contributing to the timing and efficiency of chromosome segregation Homo sapiens ? - ? 89 3.4.22.49 additional information separase is a stoichiometric inhibitor of cyclin-dependent kinase 1. This function of separase is independent of proteolytic activity Xenopus sp. ? - ? 89 3.4.22.49 additional information separase is primarily required for sister chromatid separation during mitotic and meiotic division, by cleavage of a subunit of the cohesin complex. Separase is also and perhaps indirectly important for epithelial integrity Drosophila sp. (in: flies) ? - ? 89 3.4.22.49 additional information separase shows self-cleavage upon activation. Separase is auto-cleaved between meiosis I and II in oocytes Xenopus sp. ? - ? 89 3.4.22.49 additional information the meiosis I-to-meiosis II transition in mouse oocytes requires separase activity Mus musculus ? - ? 89 3.4.22.49 additional information Cdc48 is required for the stability of Cut1/separase in mitotic anaphase Schizosaccharomyces pombe ? - ? 89 3.4.22.49 additional information separase recognizes both, a cleavage site consensus sequence as well as features outside the cleavage site Saccharomyces cerevisiae ? - ? 89 3.4.22.49 additional information TbSep is essential for the segregation of both large and minichromosomes (RNAi experiment) Trypanosoma brucei ? - ? 89 3.4.22.49 additional information does not cleave Leu-Glu-His-Asp-CHO-7-amido-4-methylcoumarin Homo sapiens ? - ? 89 3.4.22.49 additional information autocleavage of human separase is to be essential and results in conformational changes Homo sapiens ? - ? 89 3.4.22.49 additional information the enzyme interacts with Ty1-IN and does not affect the processing of the TyA-TyB polypeptide Saccharomyces cerevisiae ? - ? 89 3.4.22.49 Rad 21 + H2O - Homo sapiens ? - ? 418054 3.4.22.49 Rad21 + H2O - Homo sapiens fragments of Rad21 - ? 355597 3.4.22.49 Rad21 + H2O Rad21 is cleaved by the separase Cut1 at the metaphase-anaphase transition Schizosaccharomyces pombe fragments of Rad21 - ? 355597 3.4.22.49 Rad21 + H2O Rad21 is cleaved by Cut1 at the metaphase-anaphase transition Schizosaccharomyces pombe cleaved Rad21 - ? 370485 3.4.22.49 Rec8 + H2O - Saccharomyces cerevisiae fragments of Rac8 - ? 355596 3.4.22.49 Rec8 + H2O - Caenorhabditis elegans fragments of Rac8 - ? 355596 3.4.22.49 Rec8 + H2O cleavage of meiosis-specific Scc1 variant Rec8 is required for chromosome segregation during meiosis Saccharomyces cerevisiae fragments of Rac8 - ? 355596 3.4.22.49 Rec8 + H2O - Saccharomyces cerevisiae cleaved Rac8 - ? 370484 3.4.22.49 Rec8 + H2O - Caenorhabditis elegans cleaved Rac8 - ? 370484 3.4.22.49 Rec8 + H2O cleavage of meiosis-specific Scc1 variant Rec8 is required for chromosome segregation during meiosis Saccharomyces cerevisiae cleaved Rac8 - ? 370484 3.4.22.49 Rec8 + H2O - Schizosaccharomyces pombe ? - ? 380740 3.4.22.49 Rec8 + H2O - Saccharomyces cerevisiae ? - ? 380740 3.4.22.49 Rec8 + H2O cleavage is required for meiotic nuclear divisions in fission yeast Schizosaccharomyces pombe ? - ? 380740 3.4.22.49 Rec8 + H2O resolution of chiasmata in oocytes requires separase-mediated proteolysis. Proteolytic cleavage by separase is essential for Rec8's removal from chromosome arms and for chiasma resolution but not for the first polar body Mus musculus ? - ? 380740 3.4.22.49 Rec8 + H2O phosphorylation either of separase or cohesin is necessary for Rec8 cleavage Saccharomyces cerevisiae fragments of Rec8 - ? 410983 3.4.22.49 Rec8 + H2O the phosphorylations of Rec8 at S412 and the surrounding sites, which are most probably dependent on CK1, are essential for the cleavability of Rec8 by separase Homo sapiens fragments of Rec8 - ? 410983 3.4.22.49 Rec8 + H2O Rec8 is a meiosis-specific alpha-kleisin subunit of cohesion, phosphorylation by polo-like kinase 1 of either Rec8 or separase, or both, promotes Rec8 cleavage. Separase prefers to cleave at R454 Homo sapiens fragments of Rec8 - ? 410983 3.4.22.49 Rec8 + H2O phosphorylation either of separase or cohesin is necessary for Rec8 cleavage Saccharomyces cerevisiae SK1 fragments of Rec8 - ? 410983 3.4.22.49 Scc1 + H2O - Saccharomyces cerevisiae fragments of Scc1 - ? 355594 3.4.22.49 Scc1 + H2O separase Esp1 cleaves Scc1, the central subunit of the chromosomal cohesin complex during mitosis Saccharomyces cerevisiae fragments of Scc1 - ? 355594 3.4.22.49 Scc1 + H2O Scc1 is a subunit of cohesion, centrosomal Scc1 is cleaved by separase coincidentally with chromatin Scc1 Homo sapiens fragments of Scc1 - ? 355594 3.4.22.49 Scc1 + H2O Scc1 is an alpha-kleisin subunit of cohesin Homo sapiens fragments of Scc1 - ? 355594 3.4.22.49 Scc1 + H2O - Homo sapiens cleaved Scc1 - ? 370482 3.4.22.49 Scc1 + H2O - Saccharomyces cerevisiae cleaved Scc1 - ? 370482 3.4.22.49 Scc1 + H2O - Schizosaccharomyces pombe cleaved Scc1 - ? 370482 3.4.22.49 Scc1 + H2O Esp1 cleaves Scc1, the central subunit of the chromosomal cohesin complex during mitosis Saccharomyces cerevisiae cleaved Scc1 - ? 370482 3.4.22.49 Scc1 + H2O Scc1 contains 2 separase cleavage sites Homo sapiens cleaved Scc1 - ? 370482 3.4.22.49 Scc1 + H2O cleavage at 2 defined sites results in rapid destabilization of Scc1 and its dissociation from chromosomes Saccharomyces cerevisiae cleaved Scc1 - ? 370482 3.4.22.49 Scc1 + H2O - Saccharomyces cerevisiae ? - ? 431190 3.4.22.49 Scc1 + H2O - Mus musculus ? - ? 431190 3.4.22.49 Scc1 + H2O - Homo sapiens ? - ? 431190 3.4.22.49 Slk19 + H2O separase cleaves the kinetochore-associated protein Slk19 in anaphase Saccharomyces cerevisiae fragments of Slk19 - ? 355595 3.4.22.49 Slk19 + H2O - Saccharomyces cerevisiae cleaved Slk19 - ? 370483 3.4.22.49 Slk19 + H2O separase cleaves the kinetochore-associated protein Slk19 in metaphase-to-anaphase transition Saccharomyces cerevisiae cleaved Slk19 - ? 370483 3.4.22.49 Slk19 + H2O a protein implicated in the mitotic exit via its role in the stabilization of spindle in budding yeast Saccharomyces cerevisiae ? - ? 418077 3.4.22.49 SYN1 + H2O the enzyme plays an essential role in embryo development. The enzyme is required for the removal of cohesin from meiotic chromosomes. The cleavage of SYN1 by separase is responsible for the release of sister chromatid cohesion during meiosis Arabidopsis thaliana ? - ? 380768 3.4.22.49 [Cy5-D-R-E-I-M-R]2-rhodamine-110 + H2O - Homo sapiens 2 [Cy5-D-R-E-I-M-R] + rhodamine-110 - ? 450869