3.4.22.10 3-aminobenzoyl-Ala-Ala-Ile-Lys-Ala-Gly-Ala-Arg + H2O - Streptococcus pyogenes ? - ? 65213 3.4.22.10 3-aminobenzoyl-Ala-Ala-Ile-Lys-Ala-Gly-Ala-Arg + H2O - Streptococcus pyogenes B220 ? - ? 65213 3.4.22.10 3-aminobenzoyl-Ala-Glu-Ile-Lys-Gln-Pro-Val-Val + H2O - Streptococcus pyogenes ? - ? 65217 3.4.22.10 3-aminobenzoyl-Asp-Lys-Val-Asn-Leu-Gly-Gly-Glu + H2O - Streptococcus pyogenes ? - ? 65214 3.4.22.10 3-aminobenzoyl-Asp-Lys-Val-Asn-Leu-Gly-Gly-Glu + H2O - Streptococcus pyogenes B220 ? - ? 65214 3.4.22.10 3-aminobenzoyl-Glu-Gln-Ile-Lys-Glu-Asn-Lys-Lys + H2O - Streptococcus pyogenes ? - ? 65215 3.4.22.10 3-aminobenzoyl-Glu-Gln-Ile-Lys-Glu-Asn-Lys-Lys + H2O - Streptococcus pyogenes B220 ? - ? 65215 3.4.22.10 3-aminobenzoyl-Thr-Thr-Thr-Ala-Gly-Thr-Ala-Glu + H2O - Streptococcus pyogenes ? - ? 65216 3.4.22.10 3-aminobenzoyl-Thr-Thr-Thr-Ala-Gly-Thr-Ala-Glu + H2O - Streptococcus pyogenes B220 ? - ? 65216 3.4.22.10 acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin + H2O - Streptococcus pyogenes ? - ? 433380 3.4.22.10 acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin + H2O - Streptococcus pyogenes ATCC 10782 ? - ? 433380 3.4.22.10 alpha1-antitrypsin + H2O - Streptococcus pyogenes ? - ? 16752 3.4.22.10 azocasein + H2O - Streptococcus pyogenes ? - ? 15347 3.4.22.10 benzyloxycarbonyl-Ala + H2O - Streptococcus sp. ? - ? 65204 3.4.22.10 benzyloxycarbonyl-Glu-p-nitrophenyl ester + H2O - Streptococcus sp. ? - ? 65207 3.4.22.10 benzyloxycarbonyl-Glu-Phe + H2O - Streptococcus sp. ? - ? 65202 3.4.22.10 benzyloxycarbonyl-Gly-Gly-p-nitrophenyl ester + H2O - Streptococcus sp. ? - ? 65224 3.4.22.10 benzyloxycarbonyl-Gly-p-nitrophenyl ester + H2O - Streptococcus sp. benzyloxycarbonyl-Gly + p-nitrophenol - ? 65211 3.4.22.10 benzyloxycarbonyl-Nle-p-nitrophenyl ester + H2O - Streptococcus sp. ? - ? 65206 3.4.22.10 benzyloxycarbonyl-Nle-Phe + H2O - Streptococcus sp. ? - ? 65203 3.4.22.10 benzyloxycarbonyl-Val-Arg-4-methylcoumarinyl-7-amide + H2O - Streptococcus pyogenes ? - ? 65212 3.4.22.10 benzyloxycarbonyl-Val-Arg-4-methylcoumarinyl-7-amide + H2O - Streptococcus pyogenes B220 ? - ? 65212 3.4.22.10 Bovine serum albumin + H2O - Streptococcus pyogenes ? - ? 16295 3.4.22.10 Bovine serum albumin + H2O - Streptococcus pyogenes B220 ? - ? 16295 3.4.22.10 C5a peptidase + H2O cleavage sites are APA-K, AVI-D, SGTS, and C-terminally Streptococcus pyogenes ? - ? 417907 3.4.22.10 casein + H2O - Streptococcus pyogenes ? - ? 15445 3.4.22.10 casein + H2O - Streptococcus pyogenes M1 GAS ? - ? 15445 3.4.22.10 casein + H2O bovine Streptococcus pyogenes ? - ? 15445 3.4.22.10 casein + H2O - Streptococcus pyogenes M1 GAS HSC5 ? - ? 15445 3.4.22.10 cinnamoyl-Gly-p-nitrophenyl ester + H2O - Streptococcus sp. cinnamoyl-Gly + 4-nitrophenol - ? 65223 3.4.22.10 complement component C3 + H2O - Streptococcus pyogenes ? - ? 366556 3.4.22.10 complement factor C3b + H2O cleavage sites are RTL-D, NHK-L, LAR-S, and VEL-I Streptococcus pyogenes ? - ? 411892 3.4.22.10 D-Ile-Pro-Arg-p-nitroanilide + H2O - Streptococcus pyogenes ? - ? 65218 3.4.22.10 decorin + H2O - Streptococcus pyogenes ? - ? 384574 3.4.22.10 E-cadherin + H2O - Streptococcus pyogenes ? - ? 367535 3.4.22.10 EndoS + H2O cleavage site is VML-K Streptococcus pyogenes ? - ? 417940 3.4.22.10 FAAIKAGARY + H2O - Streptococcus pyogenes FAAIK + AGARY - ? 449907 3.4.22.10 Fba + H2O a cell surface factor H- and factor H-like protein 1-binding protein of epithelial human cells, the enzyme inhibits complement regulatory protein factor H- and factor H-like protein 1 binding by proteolysis of the Fba protein, overview Streptococcus pyogenes ? - ? 383151 3.4.22.10 Fba + H2O a cell surface factor H- and factor H-like protein 1-binding protein of epithelial human cells Streptococcus pyogenes ? - ? 383151 3.4.22.10 Fba protein + H2O - Streptococcus pyogenes ? - ? 417945 3.4.22.10 Fibrinogen + H2O human fibrinogen Streptococcus pyogenes ? - ? 15619 3.4.22.10 Fibrinogen + H2O cleavage sites are C-terminally in Aalpha chains Streptococcus pyogenes ? - ? 15619 3.4.22.10 Fibronectin + H2O - Streptococcus pyogenes ? - ? 15394 3.4.22.10 H-kininogen + H2O - Streptococcus pyogenes biologically active kinins - ? 65230 3.4.22.10 H-kininogen + H2O cleavage sites are LMK-R and PFR-S Streptococcus pyogenes ? - ? 417958 3.4.22.10 human CXCL-1 + H2O the cleavage site is between lysine residues K60 and K61 Streptococcus pyogenes ? - ? 417964 3.4.22.10 human CXCL-1 + H2O the cleavage site is between lysine residues K60 and K61 Streptococcus pyogenes H292 ? - ? 417964 3.4.22.10 human CXCL-2 + H2O the cleavage site is between lysine residues K60 and K61 Streptococcus pyogenes ? - ? 417965 3.4.22.10 human CXCL-2 + H2O the cleavage site is between lysine residues K60 and K61 Streptococcus pyogenes H292 ? - ? 417965 3.4.22.10 human CXCL-6 + H2O - Streptococcus pyogenes ? - ? 417966 3.4.22.10 human CXCL-6 + H2O - Streptococcus pyogenes H292 ? - ? 417966 3.4.22.10 human CXCL-8 + H2O - Streptococcus pyogenes ? - ? 417967 3.4.22.10 human CXCL-8 + H2O - Streptococcus pyogenes H292 ? - ? 417967 3.4.22.10 human CXCL10 + H2O - Streptococcus pyogenes ? - ? 417968 3.4.22.10 Human fibrinogen + H2O processing Streptococcus pyogenes ? - ? 17069 3.4.22.10 Human fibrinogen + H2O release and processing, the enzyme abolishes the binding of Streptococcus pyogenes to full-length or 30 kDa fragment of human fibrinogen Fn, kinetics overview Streptococcus pyogenes ? - ? 17069 3.4.22.10 human interleukin 1beta precursor + H2O - Streptococcus pyogenes human interleukin 1beta + human interleukin 1beta signal peptide - ? 384896 3.4.22.10 IgA + H2O degradation of the carboxy-terminal part of the heavy chain Streptococcus pyogenes ? - ? 366585 3.4.22.10 IgA + H2O efficiently cleaved under reducing conditions but not under nonreducing conditions Streptococcus pyogenes ? - ? 366585 3.4.22.10 IgD + H2O degradation of the carboxy-terminal part of the heavy chain Streptococcus pyogenes ? - ? 366586 3.4.22.10 IgE + H2O degradation of the carboxy-terminal part of the heavy chain Streptococcus pyogenes ? - ? 366587 3.4.22.10 IgG + H2O cleaves off the Fc part of antigen-bound IgG, the cleavage of IgG is not species restricted Streptococcus sp. ? - ? 366194 3.4.22.10 IgG + H2O the ability to cleave off the Fc part of antigen-bound IgG contributes to the escape of group A streptococci from opsonophygocytosis while not interfering with the formation of a host-like coat by unspecific IgG binding Streptococcus sp. ? - ? 366194 3.4.22.10 IgG + H2O efficiently cleaved under reducing conditions but not under nonreducing conditions Streptococcus pyogenes ? - ? 366194 3.4.22.10 IgG + H2O cleaves off the Fc part of antigen-bound IgG, the cleavage of IgG is not species restricted Streptococcus sp. T1BRB ? - ? 366194 3.4.22.10 IgG + H2O the ability to cleave off the Fc part of antigen-bound IgG contributes to the escape of group A streptococci from opsonophygocytosis while not interfering with the formation of a host-like coat by unspecific IgG binding Streptococcus sp. T1BRB ? - ? 366194 3.4.22.10 IgG + H2O the enzyme cleaves IgG in the flexible hinge region of the IgG heavy chain, generating two Fab fragments and Fc fragment. SpeB cleaves the heavy chain at a defined site between glycine residues 236 and 237 Streptococcus pyogenes Fab fragment + Gc fragments - ? 366584 3.4.22.10 IgM + H2O degradation of the carboxy-terminal part of the heavy chain Streptococcus pyogenes ? - ? 366588 3.4.22.10 IgM + H2O efficiently cleaved under reducing conditions but not under nonreducing conditions Streptococcus pyogenes ? - ? 366588 3.4.22.10 immunoglobulin A + H2O cleavage sites are the heavy chains Streptococcus pyogenes ? - ? 365833 3.4.22.10 immunoglobulin D + H2O cleavage sites are the heavy chains Streptococcus pyogenes ? - ? 417972 3.4.22.10 immunoglobulin E + H2O cleavage sites are the heavy chains Streptococcus pyogenes ? - ? 417973 3.4.22.10 immunoglobulin G + H2O cleavage site is LLG-G in gamma-chain Streptococcus pyogenes ? - ? 366852 3.4.22.10 immunoglobulin G + H2O non-immune binding of IgG to the bacterial surface is followed by the proteolytic cleavage of the antibody by the IgG-endopeptidase IdeS. IdeS generated 1/2Fc fragments do not compete efficiently with intact IgG in binding to the bacterial surface and rapid dissociation of 1/2Fc allows binding of new IgG. A correlated binding and proteolytic cleavage of IgG increases the probability that the bacteria can resist specific IgG, despite the presence of a large excess of non-specific IgG in the circulation. As a consequence of IdeS activity, circulating 1/2Fc fragments are generated. These 1/2Fc fragments are shown to be biological active by acting as priming agents for polymorphonuclear leucocytes Streptococcus pyogenes F(ab')2 + 1/2Fc - ? 405479 3.4.22.10 immunoglobulin G + H2O IdeS cleaves IgG by hydrolyzing the peptide bond between two glycine residues in the hinge region of IgG, generating one F(ab')2 fragment and two 1/2 Fc fragments Streptococcus pyogenes F(ab')2 fragment + 1/2 Fc fragments - ? 412186 3.4.22.10 immunoglobulin M + H2O cleavage sites are the heavy chains Streptococcus pyogenes ? - ? 402703 3.4.22.10 insulin + H2O reduced carboxymethylated phenylalanine chain, one of the most rapidly hydrolyzed Phe-Tyr linkages is in the Phe-Phe-Tyr sequence Streptococcus sp. ? - ? 36836 3.4.22.10 insulin beta chain + H2O reduced and carboxylated substrate, cleavage of the linkage Phe25-Tyr26 Streptococcus pyogenes ? - ? 384916 3.4.22.10 interleukin-1beta precursor + H2O - Streptococcus sp. interleukin-1beta + interleukin-1beta propeptide - ? 65233 3.4.22.10 interleukin-1beta precursor + H2O - Streptococcus sp. T10BW interleukin-1beta + interleukin-1beta propeptide - ? 65233 3.4.22.10 Laminin + H2O - Streptococcus pyogenes ? - ? 16571 3.4.22.10 M protein + H2O - Streptococcus pyogenes ? - ? 417996 3.4.22.10 additional information an unprotonated imidazole ring and the protonated form of the single sulfhydryl group are essential for activity Streptococcus pyogenes ? - ? 89 3.4.22.10 additional information the enzyme also at low concentrations efficiently removes M1 protein and protein H from the streptococcal surface Streptococcus pyogenes ? - ? 89 3.4.22.10 additional information the enzyme activates a 66000 Da matrix metalloprotease produced by human endothelial cells, a process that may contribute to endothelial cell damage, tissue destruction, and hemodynamic derangement Streptococcus pyogenes ? - ? 89 3.4.22.10 additional information by removal of M1 protein and protein H from the streptococcal surface the enzyme regulates the bacterial cell-cell interactions, and perhaps also interactions with epithelial cells. Through expression of streptopain the enzyme can modify the composition of the surface proteins in response to environmental conditions. Such a mechanism will facilitate the adaption of the bacterium to its host Streptococcus pyogenes ? - ? 89 3.4.22.10 additional information the enzyme can enhance the invasion ability of group A streptococci in human respiratory epithelial cells Streptococcus pyogenes ? - ? 89 3.4.22.10 additional information the expression of the enzyme contributes to soft tissue pathology, including necrosis, and is required for efficient systemic dissemination of the organism from the initial site of skin inoculation Streptococcus pyogenes ? - ? 89 3.4.22.10 additional information release of biological active kinins from kininogens present in human plasma Streptococcus pyogenes ? - ? 89 3.4.22.10 additional information the enzyme is an important virulence factor Streptococcus pyogenes ? - ? 89 3.4.22.10 additional information the enzyme is not critical for the development of tissue necrosis, bacteremia and lethal infection in a murine model of human necrotizing fascilitis Streptococcus sp. ? - ? 89 3.4.22.10 additional information critical virulence factor for invasive disease episode. Binds host cell integrins alphaVbeta3 and alphaIIbbeta3 through an RGD motif Streptococcus sp. ? - ? 89 3.4.22.10 additional information the enzyme is secreted under conditions of starvation and may be involved in nutrient acquisition Streptococcus pyogenes ? - ? 89 3.4.22.10 additional information thus, although IgG might by a substrate for SpeB under certain environmental conditions, it seems unlikely that SpeB is part of the first line of defense against specific antibodies. Even though SpeB might not be directly involved in the attenuation of the adaptive immune response, its proteolytic activity towards streptococcal surface proteins, including IgG-binding proteins and the streptococcal C5a peptidase, could certainly be important for the modulation of the complement system Streptococcus pyogenes ? - ? 89 3.4.22.10 additional information streptopain shows strepadhesin activity, independent of protease activity, mediated by a cell surface adhesin and controlled by the multiple gene regulator Mga Streptococcus pyogenes ? - ? 89 3.4.22.10 additional information the enzyme can induce apoptosis in A549 cells, the induction of apoptosis in cells requires the protease activity and the propper enzyme size of 28 kDa, cell binding activity of processed and unprocessed wild-type and mutant enzymes, the induction can be prevented by inhibition of caspase-8, induction cascade, overview Streptococcus pyogenes ? - ? 89 3.4.22.10 additional information the enzyme inactivates the metabolic activity of polymorphonuclear cells, the enzyme causes mitochondria damage to polymorphonuclear cells preventing phagocytosis of group A Streptococcus, it is essential for bacterial survival in blood, mechanism, overview Streptococcus pyogenes ? - ? 89 3.4.22.10 additional information the enzyme is a virulence factor of Streptococcus pyogenes inducing the release of histamine in mast cells, basophils, and mononuclear cells, and increasing capillary permeability and histamine release in skin of guinea pigs, the recombinant enzyme shows mitogenic activity with human T-cells Streptococcus pyogenes ? - ? 89 3.4.22.10 additional information cleavage site specificity, overview, the enzyme prefers cleavage of Phe-Tyr bonds, the enzyme shows poor activity with trypsin substrates fibrin, casein, and gelatin from human and rabbit Streptococcus pyogenes ? - ? 89 3.4.22.10 additional information Spi and the Spe B pro-peptide both bind to mature Spe B, but are no substrates Streptococcus pyogenes ? - ? 89 3.4.22.10 additional information the enzyme performs autolytic activity and also cleaves the inactive mutant C192S Streptococcus pyogenes ? - ? 89 3.4.22.10 additional information the enzyme performs autolytic processing to the mature protein, interaction overview Streptococcus pyogenes ? - ? 89 3.4.22.10 additional information does not cleave streptococcal mitogenic exotoxins SpeA and SpeJ Streptococcus pyogenes ? - ? 89 3.4.22.10 additional information SPE B is able to autoactivate Streptococcus pyogenes ? - ? 89 3.4.22.10 additional information the three-dimensional structure and backbone dynamics of the 28 kDa mature SPE B (mSPE B) is determined: Interactions between the C-terminal loop and the active site residues in mSPE B are observed. The structural differences between mSPE B and zymogen proSPE B are the conformation of the C-terminal loop and the orientation of the catalytic His-195 residue. Dynamics analysis of mSPEB and the mSPEB/inhibitor complexes show that the catalytic and C-terminal loops are the most flexible regions, suggesting that the flexible C-terminal loop of SPE B may play an important role in controlling the substrate binding, resulting in its broad substrate specificity Streptococcus pyogenes ? - ? 89 3.4.22.10 additional information CXCL9 is quite resistant to hydrolysis by SpeB Streptococcus pyogenes ? - ? 89 3.4.22.10 additional information incubation of mature SpeB in 20% (v/v) plasma for 24 h at 37°C does not cause degradation of the protein Streptococcus pyogenes ? - ? 89 3.4.22.10 additional information SpeB has no immunoglobulin-degrading activity in human plasma or blood Streptococcus pyogenes ? - ? 89 3.4.22.10 additional information streptopain can cleave a wide range of human proteins, including immunoglobulins, the complement activation system, chemokines, and structural proteins Streptococcus pyogenes ? - ? 89 3.4.22.10 additional information incubation of mature SpeB in 20% (v/v) plasma for 24 h at 37°C does not cause degradation of the protein Streptococcus pyogenes Alab49 ? - ? 89 3.4.22.10 additional information Spi and the Spe B pro-peptide both bind to mature Spe B, but are no substrates Streptococcus pyogenes B220 ? - ? 89 3.4.22.10 additional information the enzyme can induce apoptosis in A549 cells, the induction of apoptosis in cells requires the protease activity and the propper enzyme size of 28 kDa, cell binding activity of processed and unprocessed wild-type and mutant enzymes, the induction can be prevented by inhibition of caspase-8, induction cascade, overview Streptococcus pyogenes A-20 ? - ? 89 3.4.22.10 additional information the enzyme performs autolytic activity and also cleaves the inactive mutant C192S Streptococcus pyogenes A-20 ? - ? 89 3.4.22.10 additional information incubation of mature SpeB in 20% (v/v) plasma for 24 h at 37°C does not cause degradation of the protein Streptococcus pyogenes AP1 ? - ? 89 3.4.22.10 mouse CXCL-1 + H2O the cleavage site is between lysine residues K65 and K66 Streptococcus pyogenes ? - ? 418009 3.4.22.10 mouse CXCL-1 + H2O the cleavage site is between lysine residues K65 and K66 Streptococcus pyogenes H292 ? - ? 418009 3.4.22.10 mouse CXCL-2 + H2O the cleavage site is between lysine residues K65 and K66 Streptococcus pyogenes ? - ? 418010 3.4.22.10 N-benzoyl-Gly-p-nitrophenyl ester + H2O - Streptococcus sp. N-benzoyl-Gly + 4-nitrophenol - ? 65219 3.4.22.10 N-benzyloxycarbonyl-Ala-p-nitrophenyl ester + H2O - Streptococcus sp. N-benzyloxycarbonyl-Ala + 4-nitrophenol - ? 65225 3.4.22.10 N-benzyloxycarbonyl-Gly + H2O - Streptococcus sp. ? - ? 65205 3.4.22.10 N-benzyloxycarbonyl-Gly-p-nitrophenyl ester + H2O - Streptococcus sp. N-benzyloxycarbonyl-Gly + 4-nitrophenol - ? 65220 3.4.22.10 Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester + H2O - Streptococcus sp. ? - ? 65228 3.4.22.10 Nalpha-benzyloxycarbonyl-Lys-Phe + H2O - Streptococcus sp. ? - ? 65198 3.4.22.10 Nalpha-benzyloxycarbonyl-Lys-phenyl ester + H2O - Streptococcus sp. Nalpha-benzyloxycarbonyl-Lys + phenol - ? 454017 3.4.22.10 Nalpha-benzyloxycarbonyl-Nepsilon-t-butyloxycarbonyl-Lys-Phe + H2O - Streptococcus sp. ? - ? 65201 3.4.22.10 Nalpha-benzyloxycarbonyl-Nepsilon-tosyl-Lys-p-nitrophenyl ester + H2O - Streptococcus sp. Nalpha-benzyloxycarbonyl-Nepsilon-tosyl-Lys + p-nitrophenol - ? 65210 3.4.22.10 Nalpha-benzyloxycarbonyl-Phe-Phe + H2O - Streptococcus sp. ? - ? 65200 3.4.22.10 Nalpha-benzyloxycarbonyl-Phe-Tyr + H2O - Streptococcus sp. ? - ? 65199 3.4.22.10 Nepsilon-benzyloxycarbonyl-Lys-p-nitrophenyl ester + H2O - Streptococcus sp. Nepsilon-benzyloxycarbonyl-Lys + 4-nitrophenol - ? 65209 3.4.22.10 occludin + H2O - Streptococcus pyogenes ? - ? 393042 3.4.22.10 p-nitrobenzyloxycarbonyl-Gly-p-nitrophenyl ester + H2O - Streptococcus sp. p-nitrobenzyloxycarbonyl-Gly + 4-nitrophenol - ? 65221 3.4.22.10 p-nitrophenyl-benzyloxycarbonyl-L-Ala + H2O - Streptococcus sp. ? - ? 65227 3.4.22.10 plasminogen + H2O - Streptococcus pyogenes ? - ? 74447 3.4.22.10 pro-interleukin-1beta + H2O cleavage site is YVH-D Streptococcus pyogenes interleukin-1beta + ? - ? 393322 3.4.22.10 properdin + H2O - Streptococcus pyogenes ? - ? 383329 3.4.22.10 properdin + H2O cleavage of properdin leads to inhibition of complement-mediated opsonophagocytosis and bacterial killing by neutrophils, alternative complement pathway overview Streptococcus pyogenes ? - ? 383329 3.4.22.10 proSPE B C47S + H2O the proSPE B C47S mutant is also be used as the substrate for activity assay because it does not exhibit any enzyme activity and exists as a 42 kDa zymogen Streptococcus pyogenes ? - ? 406501 3.4.22.10 proSpeB + H2O autoactivation Streptococcus pyogenes M1 GAS SpeB + ? - ? 393353 3.4.22.10 proSpeB + H2O autoactivation Streptococcus pyogenes M1 GAS HSC5 SpeB + ? - ? 393353 3.4.22.10 protein F1 + H2O - Streptococcus pyogenes ? - ? 383341 3.4.22.10 protein F1 + H2O SpeB modulates fibronectin-dependent internalization of Streptococcus pyogenes by efficient proteolysis of cell-wall-anchored protein F1, e.g. in human pharyngeal epithelial cells Detroit 562, while proteins H and M1 are protected by plasma proteins, overview Streptococcus pyogenes ? - ? 383341 3.4.22.10 protein F1 + H2O cell-wall-anchored protein F1 Streptococcus pyogenes ? - ? 383341 3.4.22.10 protein H + H2O - Streptococcus pyogenes ? - ? 418047 3.4.22.10 resorufin-labeled casein + H2O - Streptococcus pyogenes ? - ? 385673 3.4.22.10 Sda1 + H2O - Streptococcus pyogenes ? - ? 418072 3.4.22.10 Slr + H2O - Streptococcus pyogenes ? - ? 418078 3.4.22.10 SmeZ + H2O - Streptococcus pyogenes ? - ? 418082 3.4.22.10 SpeBz + H2O auto-cleavage sites are AIK-A, KVN-L, QIK-E, TYA-G, and EIK-Q Streptococcus pyogenes mature SpeB + ? - ? 418084 3.4.22.10 streptococcal mitogenic exotoxin SmeZ + H2O cleaves between glutamate and glycine residues Streptococcus pyogenes ? - ? 393549 3.4.22.10 streptococcal mitogenic exotoxin SpeG + H2O partial degradation, SpeB cleaves between glutamate and glycine residues Streptococcus pyogenes ? - ? 393550 3.4.22.10 streptococcal mitogenic exotoxin Z + H2O - Streptococcus pyogenes ? - ? 432960 3.4.22.10 streptokinase + H2O - Streptococcus pyogenes ? - ? 418088 3.4.22.10 streptolysin O + H2O - Streptococcus pyogenes ? - ? 418089 3.4.22.10 t-butyloxycarbonyl-Gly-p-nitrophenyl ester + H2O - Streptococcus sp. t-butyloxycarbonyl-Gly + 4-nitrophenol - ? 65222 3.4.22.10 tert-butyloxycarbonyl-Ala-p-nitrophenyl ester + H2O - Streptococcus sp. tert-butyloxycarbonyl-Ala + 4-nitrophenol - ? 65226 3.4.22.10 urokinase receptor + H2O cleavage site is close to the GPI anchor Streptococcus pyogenes ? - ? 418116 3.4.22.10 Vitronectin + H2O - Streptococcus pyogenes ? - ? 16775