3.4.18.1 Glucagon + H2O - Bos taurus ? - ? 15578 3.4.18.1 Hemoglobin + H2O - Bos taurus ? - ? 15450 3.4.18.1 additional information no cleavage when Pro in the last or penultimate position Bos taurus ? - ? 89 3.4.18.1 Proteins + H2O - Bos taurus ? - ? 369561 3.4.18.1 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O - Fasciola gigantica benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin - ? 361886 3.4.18.1 additional information in addition, enzyme shows cysteine endopeptidase activity degrading gelatin, IgG and type I collagen Fasciola gigantica ? - ? 89 3.4.18.1 (2,4-dinitrophenyl)-GFFGW + H2O - Homo sapiens (2,4-dinitrophenyl)-GFFG + L-Trp - ? 365053 3.4.18.1 (2,4-dinitrophenyl)-GFFRW + H2O - Homo sapiens (2,4-dinitrophenyl)-GFFR + L-Trp - ? 365052 3.4.18.1 (2,4-dinitrophenyl)-GFFW + H2O - Homo sapiens (2,4-dinitrophenyl)-GFF + L-Trp - ? 365056 3.4.18.1 (2,4-dinitrophenyl)-GFRFW + H2O - Homo sapiens (2,4-dinitrophenyl)-GFR + L-Phe-L-Trp - ? 365057 3.4.18.1 (2,4-dinitrophenyl)-GFRW + H2O - Homo sapiens (2,4-dinitrophenyl)-GFR + L-Trp - ? 365055 3.4.18.1 (2,4-dinitrophenyl)-GRFFW + H2O - Homo sapiens (2,4-dinitrophenyl)-GRFF + L-Trp - ? 365054 3.4.18.1 (2-aminobenzoyl)-FFF + H2O - Homo sapiens (2-aminobenzoyl)-FF + L-Phe - ? 365059 3.4.18.1 (2-aminobenzoyl)-FFFA + H2O - Homo sapiens (2-aminobenzoyl)-FFF + L-Ala - ? 365060 3.4.18.1 (2-aminobenzoyl)-FFFP + H2O - Homo sapiens (2-aminobenzoyl)-FFF + Pro - ? 365062 3.4.18.1 (2-aminobenzoyl)-FFFR + H2O - Homo sapiens (2-aminobenzoyl)-FFF + L-Arg - ? 365061 3.4.18.1 (2-aminobenzoyl)-FFFR-NH2 + H2O - Homo sapiens (2-aminobenzoyl)-FFF + Arg-amide - ? 365066 3.4.18.1 (2-aminobenzoyl)-FFFW + H2O - Homo sapiens (2-aminobenzoyl)-FFF + L-Trp - ? 365058 3.4.18.1 (2-aminobenzoyl)-FFGW + H2O - Homo sapiens (2-aminobenzoyl)-FFG + L-Trp - ? 365064 3.4.18.1 (2-aminobenzoyl)-FFRW + H2O - Homo sapiens (2-aminobenzoyl)-FFR + L-Trp - ? 365063 3.4.18.1 (2-aminobenzoyl)-FFRW-NH2 + H2O - Homo sapiens (2-aminobenzoyl)-FFR + L-Trp-amide - ? 365065 3.4.18.1 (2-aminobenzoyl)-FRFW-NH2 + H2O - Homo sapiens (2-aminobenzoyl)-FR + Phe-Trp-amide - ? 365067 3.4.18.1 2-aminobenzoyl-Ala-Arg-4-nitrophenylalanine + H2O kcat/Km is 20/mM * s Homo sapiens 2-aminobenzoyl-Ala-Arg + 4-nitrophenylalanine - ? 380912 3.4.18.1 2-aminobenzoyl-Arg-Arg-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-Pro + H2O - Homo sapiens ? - ? 453997 3.4.18.1 2-aminobenzoyl-Arg-Arg-4-nitrophenylalanine + H2O kcat/Km is 5.2/mM * s Homo sapiens 2-aminobenzoyl-Arg-Arg + 4-nitrophenylalanine - ? 380914 3.4.18.1 2-aminobenzoyl-Asn-Arg-4-nitrophenylalanine + H2O kcat/Km is 17/mM * s Homo sapiens 2-aminobenzoyl-Asn-Arg + 4-nitrophenylalanine - ? 380915 3.4.18.1 2-aminobenzoyl-Asp-Arg-4-nitrophenylalanine + H2O kcat/Km is 16/mM * s Homo sapiens 2-aminobenzoyl-Asp-Arg + 4-nitrophenylalanine - ? 380916 3.4.18.1 2-aminobenzoyl-Cys-Arg-4-nitrophenylalanine + H2O kcat/Km is 11/mM * s Homo sapiens 2-aminobenzoyl-Cys-Arg + 4-nitrophenylalanine - ? 380917 3.4.18.1 2-aminobenzoyl-Gln-Arg-4-nitrophenylalanine + H2O kcat/Km is 43/mM * s Homo sapiens 2-aminobenzoyl-Gln-Arg + 4-nitrophenylalanine - ? 380918 3.4.18.1 2-aminobenzoyl-Glu-Arg-4-nitrophenylalanine + H2O kcat/Km is 27/mM * s Homo sapiens 2-aminobenzoyl-Glu-Arg + 4-nitrophenylalanine - ? 380919 3.4.18.1 2-aminobenzoyl-Gly-Arg-4-nitrophenylalanine + H2O kcat/Km is 4.4/mM * s Homo sapiens 2-aminobenzoyl-Gly-Arg + 4-nitrophenylalanine - ? 380920 3.4.18.1 2-aminobenzoyl-His-Arg-4-nitrophenylalanine + H2O kcat/Km is 4.7/mM * s Homo sapiens 2-aminobenzoyl-His-Arg + 4-nitrophenylalanine - ? 380921 3.4.18.1 2-aminobenzoyl-Ile-Arg-4-nitrophenylalanine + H2O kcat/Km is 2.7/mM * s Homo sapiens 2-aminobenzoyl-Ile-Arg + 4-nitrophenylalanine - ? 380922 3.4.18.1 2-aminobenzoyl-Leu-Arg-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-Pro + H2O - Homo sapiens ? - ? 453999 3.4.18.1 2-aminobenzoyl-Leu-Arg-4-nitrophenylalanine + H2O kcat/Km is 153/mM * s Homo sapiens 2-aminobenzoyl-Leu-Arg + 4-nitrophenylalanine - ? 380923 3.4.18.1 2-aminobenzoyl-Lys-Arg-4-nitrophenylalanine + H2O kcat/Km is 5.7/mM * s Homo sapiens 2-aminobenzoyl-Lys-Arg + 4-nitrophenylalanine - ? 380927 3.4.18.1 2-aminobenzoyl-Met-Arg-4-nitrophenylalanine + H2O kcat/Km is 28/mM * s Homo sapiens 2-aminobenzoyl-Met-Arg + 4-nitrophenylalanine - ? 380928 3.4.18.1 2-aminobenzoyl-Phe-Ala-4-nitrophenylalanine + H2O kcat/Km is 51/mM * s Homo sapiens 2-aminobenzoyl-Phe-Ala + 4-nitrophenylalanine - ? 380929 3.4.18.1 2-aminobenzoyl-Phe-Arg-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-Pro + H2O - Homo sapiens ? - ? 453998 3.4.18.1 2-aminobenzoyl-Phe-Arg-4-nitrophenylalanine + H2O kcat/Km is 100/mM * s Homo sapiens 2-aminobenzoyl-Phe-Arg + 4-nitrophenylalanine - ? 380930 3.4.18.1 2-aminobenzoyl-Phe-Arg-Ala + H2O kcat/Km is 10/mM * s Homo sapiens 2-aminobenzoyl-Phe-Arg + Ala - ? 380931 3.4.18.1 2-aminobenzoyl-Phe-Arg-Arg + H2O kcat/Km is 1.7/mM * s Homo sapiens 2-aminobenzoyl-Phe-Arg + Arg - ? 380932 3.4.18.1 2-aminobenzoyl-Phe-Arg-Asn + H2O kcat/Km is 5.9/mM * s Homo sapiens 2-aminobenzoyl-Phe-Arg + Asn - ? 380933 3.4.18.1 2-aminobenzoyl-Phe-Arg-Asp + H2O kcat/Km is 8.9/mM * s Homo sapiens 2-aminobenzoyl-Phe-Arg + Asp - ? 380934 3.4.18.1 2-aminobenzoyl-Phe-Arg-Cys + H2O kcat/Km is 73/mM * s Homo sapiens 2-aminobenzoyl-Phe-Arg + Cys - ? 380935 3.4.18.1 2-aminobenzoyl-Phe-Arg-Gln + H2O kcat/Km is 4.9/mM * s Homo sapiens 2-aminobenzoyl-Phe-Arg + Gln - ? 380936 3.4.18.1 2-aminobenzoyl-Phe-Arg-Glu + H2O kcat/Km is 7.7/mM * s Homo sapiens 2-aminobenzoyl-Phe-Arg + Glu - ? 380937 3.4.18.1 2-aminobenzoyl-Phe-Arg-Gly + H2O kcat/Km is 14/mM * s Homo sapiens 2-aminobenzoyl-Phe-Arg + Gly - ? 380938 3.4.18.1 2-aminobenzoyl-Phe-Arg-His + H2O kcat/Km is 5.4/mM * s Homo sapiens 2-aminobenzoyl-Phe-Arg + His - ? 380939 3.4.18.1 2-aminobenzoyl-Phe-Arg-Ile + H2O kcat/Km is 3.4/mM * s Homo sapiens 2-aminobenzoyl-Phe-Arg + Ile - ? 380940 3.4.18.1 2-aminobenzoyl-Phe-Arg-Leu + H2O kcat/Km is 7.1/mM * s Homo sapiens 2-aminobenzoyl-Phe-Arg + Leu - ? 380941 3.4.18.1 2-aminobenzoyl-Phe-Arg-Lys + H2O kcat/Km is 5.3/mM * s Homo sapiens 2-aminobenzoyl-Phe-Arg + Lys - ? 380942 3.4.18.1 2-aminobenzoyl-Phe-Arg-Met + H2O kcat/Km is 9.3/mM * s Homo sapiens 2-aminobenzoyl-Phe-Arg + Met - ? 380943 3.4.18.1 2-aminobenzoyl-Phe-Arg-Phe + H2O kcat/Km is 15/mM * s Homo sapiens 2-aminobenzoyl-Phe-Arg + Phe - ? 380944 3.4.18.1 2-aminobenzoyl-Phe-Arg-Ser + H2O kcat/Km is 48/mM * s Homo sapiens 2-aminobenzoyl-Phe-Arg + Ser - ? 380945 3.4.18.1 2-aminobenzoyl-Phe-Arg-Thr + H2O kcat/Km is 5.8/mM * s Homo sapiens 2-aminobenzoyl-Phe-Arg + Thr - ? 380946 3.4.18.1 2-aminobenzoyl-Phe-Arg-Trp + H2O kcat/Km is 8.6/mM * s Homo sapiens 2-aminobenzoyl-Phe-Arg + Trp - ? 380947 3.4.18.1 2-aminobenzoyl-Phe-Arg-Tyr + H2O kcat/Km is 8.8/mM * s Homo sapiens 2-aminobenzoyl-Phe-Arg + Tyr - ? 380948 3.4.18.1 2-aminobenzoyl-Phe-Arg-Val + H2O kcat/Km is 5.9/mM * s Homo sapiens 2-aminobenzoyl-Phe-Arg + Val - ? 380949 3.4.18.1 2-aminobenzoyl-Phe-Asn-4-nitrophenylalanine + H2O kcat/Km is 13/mM * s Homo sapiens 2-aminobenzoyl-Phe-Asn + 4-nitrophenylalanine - ? 380950 3.4.18.1 2-aminobenzoyl-Phe-Asp-4-nitrophenylalanine + H2O kcat/Km is 12/mM * s Homo sapiens 2-aminobenzoyl-Phe-Asp + 4-nitrophenylalanine - ? 380951 3.4.18.1 2-aminobenzoyl-Phe-Cys-4-nitrophenylalanine + H2O kcat/Km is 17/mM * s Homo sapiens 2-aminobenzoyl-Phe-Cys + 4-nitrophenylalanine - ? 380952 3.4.18.1 2-aminobenzoyl-Phe-Gln-4-nitrophenylalanine + H2O kcat/Km is 73/mM * s Homo sapiens 2-aminobenzoyl-Phe-Gln + 4-nitrophenylalanine - ? 380953 3.4.18.1 2-aminobenzoyl-Phe-Glu-4-nitrophenylalanine + H2O kcat/Km is 62/mM * s Homo sapiens 2-aminobenzoyl-Phe-Glu + 4-nitrophenylalanine - ? 380954 3.4.18.1 2-aminobenzoyl-Phe-Gly-4-nitrophenylalanine + H2O kcat/Km is 45/mM * s Homo sapiens 2-aminobenzoyl-Phe-Gly + 4-nitrophenylalanine - ? 380955 3.4.18.1 2-aminobenzoyl-Phe-His-4-nitrophenylalanine + H2O kcat/Km is 3.5/mM * s Homo sapiens 2-aminobenzoyl-Phe-His + 4-nitrophenylalanine - ? 380956 3.4.18.1 2-aminobenzoyl-Phe-Ile-4-nitrophenylalanine + H2O kcat/Km is 4.3/mM * s Homo sapiens 2-aminobenzoyl-Phe-Ile + 4-nitrophenylalanine - ? 380957 3.4.18.1 2-aminobenzoyl-Phe-Leu-4-nitrophenylalanine + H2O kcat/Km is 2.0/mM * s Homo sapiens 2-aminobenzoyl-Phe-Leu + 4-nitrophenylalanine - ? 380958 3.4.18.1 2-aminobenzoyl-Phe-Lys-4-nitrophenylalanine + H2O kcat/Km is 79/mM * s Homo sapiens 2-aminobenzoyl-Phe-Lys + 4-nitrophenylalanine - ? 380959 3.4.18.1 2-aminobenzoyl-Phe-Met-4-nitrophenylalanine + H2O kcat/Km is 181/mM * s Homo sapiens 2-aminobenzoyl-Phe-Met + 4-nitrophenylalanine - ? 380960 3.4.18.1 2-aminobenzoyl-Phe-Phe-4-nitrophenylalanine + H2O kcat/Km is 108/mM * s Homo sapiens 2-aminobenzoyl-Phe-Phe + 4-nitrophenylalanine - ? 380961 3.4.18.1 2-aminobenzoyl-Phe-Ser-4-nitrophenylalanine + H2O kcat/Km is 32/mM * s Homo sapiens 2-aminobenzoyl-Phe-Ser + 4-nitrophenylalanine - ? 380962 3.4.18.1 2-aminobenzoyl-Phe-Thr-4-nitrophenylalanine + H2O kcat/Km is 68/mM * s Homo sapiens 2-aminobenzoyl-Phe-Thr + 4-nitrophenylalanine - ? 380963 3.4.18.1 2-aminobenzoyl-Phe-Trp-4-nitrophenylalanine + H2O kcat/Km is 1.3/mM * s Homo sapiens 2-aminobenzoyl-Phe-Trp + 4-nitrophenylalanine - ? 380964 3.4.18.1 2-aminobenzoyl-Phe-Tyr-4-nitrophenylalanine + H2O kcat/Km is 58/mM * s Homo sapiens 2-aminobenzoyl-Phe-Tyr + 4-nitrophenylalanine - ? 380965 3.4.18.1 2-aminobenzoyl-Phe-Val-4-nitrophenylalanine + H2O kcat/Km is 4.8/mM * s Homo sapiens 2-aminobenzoyl-Phe-Val + 4-nitrophenylalanine - ? 380966 3.4.18.1 2-aminobenzoyl-Pro-Arg-4-nitrophenylalanine + H2O kcat/Km is 0.2/mM * s Homo sapiens 2-aminobenzoyl-Pro-Arg + 4-nitrophenylalanine - ? 380967 3.4.18.1 2-aminobenzoyl-Ser-Arg-4-nitrophenylalanine + H2O kcat/Km is 8.2/mM * s Homo sapiens 2-aminobenzoyl-Ser-Arg + 4-nitrophenylalanine - ? 380968 3.4.18.1 2-aminobenzoyl-Thr-Arg-4-nitrophenylalanine + H2O kcat/Km is 8.7/mM * s Homo sapiens 2-aminobenzoyl-Thr-Arg + 4-nitrophenylalanine - ? 380969 3.4.18.1 2-aminobenzoyl-Trp-Arg-4-nitrophenylalanine + H2O kcat/Km is 12/mM * s Homo sapiens 2-aminobenzoyl-Trp-Arg + 4-nitrophenylalanine - ? 380970 3.4.18.1 2-aminobenzoyl-Tyr-Arg-4-nitrophenylalanine + H2O kcat/Km is 400/mM * s Homo sapiens 2-aminobenzoyl-Tyr-Arg + 4-nitrophenylalanine - ? 380971 3.4.18.1 2-aminobenzoyl-Val-Arg-4-nitrophenylalanine + H2O kcat/Km is 31/mM * s Homo sapiens 2-aminobenzoyl-Val-Arg + 4-nitrophenylalanine - ? 380972 3.4.18.1 3-aminobenzoyl-FR-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-P + H2O - Homo sapiens 3-aminobenzoyl-FR + (2,3-diaminopropionyl)-(2,4-dinitrophenyl)-P - ? 365049 3.4.18.1 3-aminobenzoyl-LR-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-P + H2O - Homo sapiens 3-aminobenzoyl-LR + (2,3-diaminopropionyl)-(2,4-dinitrophenyl)-P - ? 365051 3.4.18.1 3-aminobenzoyl-RR-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-P + H2O - Homo sapiens 3-aminobenzoyl-RR + (2,3-diaminopropionyl)-(2,4-dinitrophenyl)-P - ? 365050 3.4.18.1 7-methoxycoumarin-4-ylacetyl-RPPGFSAFK-N-epsilon-2,4-dinitrophenol + H2O fluorescence cathepsin X/A-selective substrate Homo sapiens ? - ? 411418 3.4.18.1 Abz-Phe-Glu-Lys(Dnp)-OH + H2O - Homo sapiens ? - ? 411494 3.4.18.1 AKYNQLMRIEEELGEEARFAGHNFRNPSVL + H2O a model peptide derived from rat gamma-enolase carboxyl terminal, overview Homo sapiens ? - ? 411590 3.4.18.1 alpha-enolase + H2O cathepsin X cleaves the C-terminal dipeptide of alpha- and gamma-enolase abolishing their neurotrophic activity Homo sapiens ? - ? 410713 3.4.18.1 alpha-enolase + H2O cathepsin X cleaves the C-terminal dipeptide Homo sapiens ? - ? 410713 3.4.18.1 benzyloxycarbonyl-FR-4-methylcoumaryl-7-amide + H2O - Homo sapiens benzyloxycarbonyl-FR + 7-amino-4-methylcoumarin - ? 365069 3.4.18.1 benzyloxycarbonyl-RR-4-methylcoumaryl-7-amide + H2O - Homo sapiens benzyloxycarbonyl-RR + 7-amino-4-methylcoumarin - ? 365068 3.4.18.1 bradykinin + H2O i.e. Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg, a mainly B2 receptor Homo sapiens Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe + Arg an exclusive B1 receptor ? 15064 3.4.18.1 bradykinin + H2O the peptide is converted from a bradykinin B2 receptor ligand to a bradykinin B1 receptor specific ligand Homo sapiens ? - ? 16889 3.4.18.1 CXCL-12 + H2O CXCL-12 is a physiological substrate for secreted cathepsin X Homo sapiens ? - ? 410795 3.4.18.1 CXCL-12 + H2O chemokine CXCL-12 is a highly potent chemoattractant for HSC secreted by osteoblasts. The exo-peptidase cathepsin X gradually cleaves fifteen amino acids until proline P74 is present at the P2 position Homo sapiens ? - ? 410795 3.4.18.1 gamma-enolase + H2O cathepsin X cleaves the C-terminal dipeptide of alpha- and gamma-enolase abolishing their neurotrophic activity Homo sapiens ? - ? 410830 3.4.18.1 gamma-enolase + H2O cathepsin X cleaves the C-terminal dipeptide Homo sapiens ? - ? 410830 3.4.18.1 Hippuryl-L-Arg + H2O - Homo sapiens Hippuric acid + L-Arg - ? 15059 3.4.18.1 hippuryl-L-glutamic acid + H2O - Homo sapiens hippuric acid + L-Glu - ? 36797 3.4.18.1 KAKFAGRNPRNPLAK + H2O a model peptide derived from human alpha-enolase carboxyl terminal, overview Homo sapiens ? - ? 412202 3.4.18.1 kallidin + H2O the peptide is converted from a bradykinin B2 receptor ligand to a bradykinin B1 receptor specific ligand Homo sapiens ? - ? 380633 3.4.18.1 kallidin + H2O i.e. Lys-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg, a mainly B2 receptor Homo sapiens Lys-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe + Arg an exclusive B1 receptor ? 412203 3.4.18.1 lymphocyte function associated antigen-1 + H2O cathepsin X cleaves the beta2 cytoplasmic tail of LFA-1 inducing the intermediate affinity form of LFA-1 and alpha-actinin-1 binding. Cleavage by cathepsin X of the amino acid residues S769, E768 and A767 from the C-terminal of the b2 cytoplasmic tail of LFA-1 promotes binding of the actin-binding protein a-actinin-1 Homo sapiens ? - ? 410891 3.4.18.1 lymphocyte function associated antigen-1 + H2O cathepsin X cleaves the amino acid residues S769, E768 and A767 from the C-terminal of LFA-1 Homo sapiens ? - ? 410891 3.4.18.1 additional information the enzyme normally acts as a carboxymonopeptidase. The preference for Arg in the P1 position is so strong that cathepsin X cleaves substrates with Arg in antepenultimate position, acting also as a carboxypeptidase. A large hydrophobic residue such as Trp is preferred in the P1' position, although the enzyme cleaves all P1' residues investigated (Trp, Phe, Ala, Arg, Pro). The enzyme also cleaves the substrates with amide-blocked C-terminal carboxyl group with rates similar to that of the unblocked substrates Homo sapiens ? - ? 89 3.4.18.1 additional information active cathepsin X mediates the function of beta2 integrin receptors during cell adhesion. It could also be involved in other processes associated with beta2 integrin receptors such as phagocytosis and T cell activation Homo sapiens ? - ? 89 3.4.18.1 additional information cathepsin X plays a role not only in the chronic inflammation of gastric mucosa but also in the tumourigenesis of gastric cancer Homo sapiens ? - ? 89 3.4.18.1 additional information cathespin X is involved in phagocytosis and regulation of immune response, not involved in degradation of extracellular matrix, a proteolytic event leading to tumor cell invasion and metastasis Homo sapiens ? - ? 89 3.4.18.1 additional information cathepsin X binds to the membrane lectin endoplasmic reticulum Golgi intermediate compartment protein-53, ERGIC-53, involving the soluble luminal interaction partner multiple coagulation factor deficiency protein 2, MCFD2, which form a cargo receptor complex in the early secretory pathway, but is dispensable for enzyme binding, overview Homo sapiens ? - ? 89 3.4.18.1 additional information luminal protein-protein interactions between components of the cargo system in the endoplasmic reticulum for secretion of cargo proteins, e.g. cathepsin C or cathepsin Z, involve the cargo transport receptor ERGIC-53, i.e. endoplasmic reticulum-Golgi intermediate compartment protein of 53 kDa, with its luminal interaction partner MCFD2, i.e. multiple coagulation factor deficiency protein 2, MCFD2 is not required for the binding of cathepsin Z and cathepsin C to ERGIC-53 in vivo, overview Homo sapiens ? - ? 89 3.4.18.1 additional information procathepsin X supports integrin alphavbeta3-dependent attachment and spreading of umbilical vein endothelial cells, overvie Homo sapiens ? - ? 89 3.4.18.1 additional information the enzyme is associated with plaques in Alzheimer patients, overview Homo sapiens ? - ? 89 3.4.18.1 additional information the enzyme plays a role in immunity to pathogens including Mycobacterium tuberculosis, variation in the melanocortin 3 receptor and cathepsin Z genes play a role in the pathogenesis of tuberculosis in West African populations Homo sapiens ? - ? 89 3.4.18.1 additional information the enzyme stimulates macrophage antigen-1 receptor-dependent adhesion and phagocytosis via interaction with integrin beta2 subunit. It plays a role in regulating lymphocyte proliferation via Mac-1 and the other b2 integrin receptor, lymphocyte function-associated antigen-1. Cathepsin X has been shown to suppress proliferation of peripheral blood mononuclear cells, by activation of Mac-1, known as a suppressive factor for lymphocyte proliferation, co-localization of cathepsin X and LFA-1 enhances lymphocyte proliferation, overview Homo sapiens ? - ? 89 3.4.18.1 additional information cathepsin X interacts with the membrane lectin endoplasmic reticulum Golgi intermediate compartment protein-53, ERGIC-53 Homo sapiens ? - ? 89 3.4.18.1 additional information direct interaction between the enzyme and integrin alphavbeta3, overview Homo sapiens ? - ? 89 3.4.18.1 additional information luminal protein-protein interactions between components of the cargo system in the endoplasmic reticulum for secretion of cargo proteins, e.g. cathepsin C or cathepsin Z, involve the cargo transport receptor ERGIC-53, i.e. endoplasmic reticulum-Golgi intermediate compartment protein of 53 kDa, with its luminal interaction partner MCFD2, i.e. multiple coagulation factor deficiency protein 2, inactivation of ERGIC-53’s lectin domain by the N156A point mutation selectively decreases the interaction of ERGIC-53 with its cargo proteins cathepsin Z and cathepsin C, whereas the N156A mutation does not affect ERGIC-53 oligomerization or its interaction with MCFD2, overview Homo sapiens ? - ? 89 3.4.18.1 additional information cathepsin X acts as a monocarboxypepidase and has a strict positional and narrower substrate specificity relative to the other human cathepsins Homo sapiens ? - ? 89 3.4.18.1 additional information cathepsin X is an important regulator of LFA-1 activity, and cathepsin X-upregulated Jurkat T cells exhibit increased homotypic aggregation, cathepsin X induces polarized migration-associated morphology in Jurkat T cells, overview Homo sapiens ? - ? 89 3.4.18.1 additional information co-localization of alpha or gamma enolase and cathepsin X. Cathepsin X impairs survival and neuritogenesis of neuronal cells, e.g. it reduces PC12 cell survival and neuritogenesis Homo sapiens ? - ? 89 3.4.18.1 ortho-aminobenzoyl-Arg-Arg-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-Pro + H2O - Homo sapiens ? - ? 452936 3.4.18.1 ortho-aminobenzoyl-Lys-Arg-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-Pro + H2O - Homo sapiens ? - ? 452935 3.4.18.1 ortho-aminobenzoyl-Phe-Arg-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-Pro + H2O - Homo sapiens ? - ? 452934 3.4.18.1 profilin + H2O cathepsin X cleaves profilin 1 C-terminal Tyr139 and influences clathrin-mediated endocytosis. Tyr139 is important for proper function of profilin 1 as a tumor suppressor. Cleaving off Tyr139 prevents the binding of clathrin, a poly-L-proline ligand involved in endocytosis Homo sapiens L-tyrosine + ? - ? 448874 3.4.18.1 profilin 1 + H2O the molecular target of cathepsin X in tumor cells is profilin 1, a known tumor suppressor and regulator of actin cytoskeleton dynamics. Cathepsin X cleaves off the C-terminal Tyr139 of profilin 1, affecting binding of poly-L-proline ligands and, consequently, tumor cell migration and invasion. Tyr139 is important for proper function of profilin 1 as a tumor suppressor. Cleaving off Tyr139 prevents the binding of clathrin, a poly-L-proline ligand involved in endocytosis Homo sapiens ? - ? 431147 3.4.18.1 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide + H2O - Mus musculus benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin - ? 394266 3.4.18.1 biotinyl-KKQ20KK + H2O - Mus musculus ? within the lysosome, the major endoprotease, cathepsin L, carries out an initial attack within the polyglutamine repeat, which generates new C termini, facilitating the actions of the carboxypeptidase cathepsin Z. Extracts containing both cathespin L and Z show multiple cleavages within the polyglutamine sequence of biotinyl-KKQ20KK, generating a variety of fragments, including biotinyl-KKQ4,biotinyl-KKQ8, Q12KK, andQ16KK ? 431697 3.4.18.1 Z-Arg-Arg-7-amido-4-methylcoumarin + H2O - Paralichthys olivaceus ? - ? 402264 3.4.18.1 Z-Phe-Arg-7-amido-4-methylcoumarin + H2O - Paralichthys olivaceus ? - ? 385971 3.4.18.1 Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O 61% of the activity with benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin Patiria pectinifera Ala-Ala-Phe + 7-amino-4-methylcoumarin - ? 361588 3.4.18.1 benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O 34% of the activity with benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin Patiria pectinifera benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin - ? 362355 3.4.18.1 benzyloxycarbonyl-Leu-Leu-Glu-7-amido-4-methylcoumarin + H2O 54% of the activity with benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin Patiria pectinifera benzyloxycarbonyl-Leu-Leu-Glu + 7-amino-4-methylcoumarin - ? 411721 3.4.18.1 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O - Patiria pectinifera benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin - ? 361886 3.4.18.1 benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O 12% of the activity with benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin Patiria pectinifera benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin - ? 362763 3.4.18.1 additional information CATX is an important player in the spinal mechanisms involved in chronic pain induction and maintenance Rattus norvegicus ? - ? 89 3.4.18.1 additional information CATX is widely expressed in the brain and is implicated in several neurological conditions such as Alzheimer's disease, amyotrophic lateral sclerosis and age-related inflammation Rattus norvegicus ? - ? 89