3.2.2.8 1-beta-D-ribofuranosylnicotinamide + H2O - Pseudomonas fluorescens nicotinamide + D-ribose - ? 94861 3.2.2.8 1-beta-D-ribofuranosylthymine + H2O - Pseudomonas fluorescens D-ribose + thymine - ? 94857 3.2.2.8 4-amino-5-imidazolecarboxamide ribonucleotide + H2O - Pseudomonas fluorescens 4-amino-5-imidazolecarboxamide + D-ribose - ? 94862 3.2.2.8 4-nitrophenyl beta-D-ribofuranoside + H2O - Leishmania braziliensis 4-nitrophenol + D-ribose - ? 394168 3.2.2.8 4-nitrophenyl ribopyranoside + H2O - Escherichia coli K-12 4-nitrophenol + D-ribose - ? 381197 3.2.2.8 5-bromouridine + H2O - Escherichia coli 5-bromouracil + D-ribose - ? 94858 3.2.2.8 5-bromouridine + H2O - Pseudomonas fluorescens 5-bromouracil + D-ribose - ? 94858 3.2.2.8 5-fluorouridine + H2O - Escherichia coli 5-fluorouracil + D-ribose - ? 372132 3.2.2.8 5-fluorouridine + H2O detagged recombinant enzyme Escherichia coli K-12 5-fluorouracil + D-ribose - ? 372132 3.2.2.8 5-iodouridine + H2O - Escherichia coli 5-iodouracil + D-ribose - ? 372135 3.2.2.8 5-methyluridine + H2O - Escherichia coli 5-methyluracil + D-ribose - ? 214510 3.2.2.8 5-methyluridine + H2O - Arabidopsis thaliana D-ribose + 5-methyluracil - ? 416443 3.2.2.8 6-azauridine + H2O poor substrate Pseudomonas fluorescens 6-azauracil + D-ribose - ? 94860 3.2.2.8 6-mercaptopurine ribonucleoside + H2O - Leishmania donovani 6-mercaptopurine + D-ribose - ? 94855 3.2.2.8 a pyrimidine nucleoside + H2O - Escherichia coli D-ribose + a pyrimidine base - ? 410698 3.2.2.8 adenosine + H2O - Arabidopsis thaliana adenine + D-ribose - ? 94810 3.2.2.8 adenosine + H2O - Pseudomonas fluorescens adenine + D-ribose - ? 94810 3.2.2.8 adenosine + H2O - Escherichia coli K-12 adenine + D-ribose - ? 94810 3.2.2.8 adenosine + H2O - Leishmania braziliensis adenine + D-ribose - ? 94810 3.2.2.8 adenosine + H2O - Arabidopsis thaliana D-ribose + adenine - ? 214501 3.2.2.8 cytidine + H2O - Escherichia coli cytosine + D-ribose - ? 94851 3.2.2.8 cytidine + H2O - Penicillium chrysogenum cytosine + D-ribose - ? 94851 3.2.2.8 cytidine + H2O - Pseudomonas fluorescens cytosine + D-ribose - ? 94851 3.2.2.8 cytidine + H2O - Leishmania donovani cytosine + D-ribose - ? 94851 3.2.2.8 cytidine + H2O - Saccharolobus solfataricus cytosine + D-ribose - ? 94851 3.2.2.8 cytidine + H2O - Corynebacterium ammoniagenes cytosine + D-ribose - ? 94851 3.2.2.8 cytidine + H2O - Escherichia coli K-12 cytosine + D-ribose - ? 94851 3.2.2.8 cytidine + H2O - Leishmania braziliensis cytosine + D-ribose - ? 94851 3.2.2.8 cytidine + H2O detagged recombinant enzyme Escherichia coli K-12 cytosine + D-ribose - ? 94851 3.2.2.8 guanosine + H2O - Pseudomonas fluorescens guanine + D-ribose - ? 94811 3.2.2.8 guanosine + H2O - Escherichia coli K-12 guanine + D-ribose - ? 94811 3.2.2.8 guanosine + H2O - Leishmania braziliensis guanine + D-ribose - ? 94811 3.2.2.8 imidazoleacetic acid ribonucleotide + H2O - Pseudomonas fluorescens imidazoleacetic acid + D-ribose - ? 94863 3.2.2.8 inosine + H2O - Arabidopsis thaliana hypoxanthine + D-ribose - ? 94805 3.2.2.8 inosine + H2O - Pseudomonas fluorescens hypoxanthine + D-ribose - ? 94805 3.2.2.8 inosine + H2O - Leishmania donovani hypoxanthine + D-ribose - ? 94805 3.2.2.8 inosine + H2O - Saccharolobus solfataricus hypoxanthine + D-ribose - ? 94805 3.2.2.8 inosine + H2O - Escherichia coli K-12 hypoxanthine + D-ribose - ? 94805 3.2.2.8 inosine + H2O poor substrate Escherichia coli hypoxanthine + D-ribose - ? 94805 3.2.2.8 inosine + H2O - Saccharolobus solfataricus P2 hypoxanthine + D-ribose - ? 94805 3.2.2.8 inosine + H2O catalytic efficiency towards inosine is at least 100-fold below that for uridine Saccharolobus solfataricus ? - ? 392260 3.2.2.8 inosine + H2O - Arabidopsis thaliana D-ribose + hypoxanthine - ? 405483 3.2.2.8 inosine + H2O - Leishmania braziliensis D-ribose + hypoxanthine - ? 405483 3.2.2.8 isopentenyladenine riboside + H2O - Arabidopsis thaliana ? - ? 405515 3.2.2.8 additional information not: deoxyribothymidine Penicillium chrysogenum ? - ? 89 3.2.2.8 additional information not: adenosine and guanosine Leishmania donovani ? - ? 89 3.2.2.8 additional information enzyme is pyrimidine specific, purine nucleosides are not hydrolysed, 2'-, 3'- and 5'-deoxynucleosides are no substrates Escherichia coli ? - ? 89 3.2.2.8 additional information no substrate: deazouridine Escherichia coli ? - ? 89 3.2.2.8 additional information the enzyme is required for recycling of nitrogenous bases Escherichia coli K-12 ? - ? 89 3.2.2.8 additional information detagged recombinant enzyme: substrate specificity, no activity with purine nucleosides, and with 2'-, 3'-, and 5'-deoxynucleosides Escherichia coli K-12 ? - ? 89 3.2.2.8 additional information the pure recombinant protein exhibits highest hydrolase activity for uridine, followed by inosine and adenosine Arabidopsis thaliana ? - ? 89 3.2.2.8 additional information catalytic cycles between the open and closed conformations of RihA, stabilization of two flexible active site regions is pivotal to establish the interactions required for substrate discrimination and catalysis, involvement of the Asp10 as general base in the mechanism, role of the conserved His82 residue in modulating product release, structure-function analysis, detailed overview Escherichia coli ? - ? 89 3.2.2.8 N-D-ribosylpyrimidine - Penicillium chrysogenum pyrimidine + D-ribose - ? 369792 3.2.2.8 N-D-ribosylpyrimidine - Pisum sativum pyrimidine + D-ribose - ? 369792 3.2.2.8 N-D-ribosylpyrimidine - Pseudomonas fluorescens pyrimidine + D-ribose - ? 369792 3.2.2.8 N-D-ribosylpyrimidine - Leishmania donovani pyrimidine + D-ribose - ? 369792 3.2.2.8 N-D-ribosylpyrimidine + H2O - Penicillium chrysogenum pyrimidine + D-ribose - ? 94849 3.2.2.8 N-D-ribosylpyrimidine + H2O - Pisum sativum pyrimidine + D-ribose - ? 94849 3.2.2.8 N-D-ribosylpyrimidine + H2O - Pseudomonas fluorescens pyrimidine + D-ribose - ? 94849 3.2.2.8 N-D-ribosylpyrimidine + H2O - Leishmania donovani pyrimidine + D-ribose - ? 94849 3.2.2.8 N-ribosylpurine + H2O - Escherichia coli K-12 purine + D-ribose - r 380679 3.2.2.8 N-ribosylpyrimidine + H2O preferred substrate Escherichia coli K-12 pyrimidine + D-ribose - r 380680 3.2.2.8 purine D-ribonucleosides + H2O appreciable substrate activity if a hydroxyl or thio group is present in the 6-position or if a hydroxyl group is present in the 2- and 6-position, preference for oxo versus amino substituents Leishmania donovani purine + D-ribose - ? 94850 3.2.2.8 purine D-ribonucleosides + H2O prefers pyrimidines Pseudomonas fluorescens purine + D-ribose - ? 94850 3.2.2.8 purine D-ribonucleosides + H2O prefers pyrimidines Leishmania donovani purine + D-ribose - ? 94850 3.2.2.8 tubercidin + H2O poor substrate Pseudomonas fluorescens 1-deazaadenine + D-ribose - ? 94864 3.2.2.8 uridine + H2O - Escherichia coli uracil + D-ribose - ? 94852 3.2.2.8 uridine + H2O - Penicillium chrysogenum uracil + D-ribose - ? 94852 3.2.2.8 uridine + H2O - Pisum sativum uracil + D-ribose - ? 94852 3.2.2.8 uridine + H2O - Arabidopsis thaliana uracil + D-ribose - ? 94852 3.2.2.8 uridine + H2O - Pseudomonas fluorescens uracil + D-ribose - ? 94852 3.2.2.8 uridine + H2O - Saccharolobus solfataricus uracil + D-ribose - ? 94852 3.2.2.8 uridine + H2O - Corynebacterium ammoniagenes uracil + D-ribose - ? 94852 3.2.2.8 uridine + H2O - Escherichia coli K-12 uracil + D-ribose - ? 94852 3.2.2.8 uridine + H2O - Leishmania braziliensis uracil + D-ribose - ? 94852 3.2.2.8 uridine + H2O cleaves uridine most efficiantly Leishmania donovani uracil + D-ribose - ? 94852 3.2.2.8 uridine + H2O detagged recombinant enzyme Escherichia coli K-12 uracil + D-ribose - ? 94852 3.2.2.8 uridine + H2O the pure recombinant protein exhibits highest hydrolase activity for uridine, followed by inosine and adenosine Arabidopsis thaliana uracil + D-ribose - ? 94852 3.2.2.8 uridine + H2O - Arabidopsis thaliana D-ribose + uracil - ? 214503 3.2.2.8 xanthosine + H2O - Pseudomonas fluorescens xanthine + D-ribose - ? 94806 3.2.2.8 xanthosine + H2O - Leishmania donovani xanthine + D-ribose - ? 94806 3.2.2.8 xanthosine + H2O second best cleavage Leishmania donovani xanthine + D-ribose - ? 94806 3.2.2.8 xanthosine + H2O high hydrolytic efficiency, xanthosine is a better substrate for isoform NSH1 compared to inosine and adenosine Arabidopsis thaliana xanthine + D-ribose - ? 94806