3.2.1.41 3 glycogen + H2O - Aureobasidium pullulans 4 maltotriose - ? 361119 3.2.1.41 3 glycogen + H2O - Anaerobranca gottschalkii 4 maltotriose - ? 361119 3.2.1.41 4-nitrophenyl alpha-D-maltoheptaoside + H2O hydrolyzed in an exo-fashion Pyrococcus furiosus 4-nitrophenol + alpha-D-maltoheptaose - ? 422621 3.2.1.41 4-nitrophenyl alpha-D-maltoheptaoside + H2O hydrolyzed in an exo-fashion Thermococcus litoralis 4-nitrophenol + alpha-D-maltoheptaose - ? 422621 3.2.1.41 4-nitrophenyl alpha-D-maltoheptaoside + H2O hydrolyzed in an exo-fashion Thermococcus litoralis DSM 5473 4-nitrophenol + alpha-D-maltoheptaose - ? 422621 3.2.1.41 4-nitrophenyl alpha-D-maltotrioside + H2O hydrolyzed in an exo-fashion Pyrococcus furiosus 4-nitrophenol + alpha-D-maltotriose - ? 422622 3.2.1.41 4-nitrophenyl alpha-D-maltotrioside + H2O hydrolyzed in an exo-fashion Thermococcus litoralis 4-nitrophenol + alpha-D-maltotriose - ? 422622 3.2.1.41 42-O-beta-D-galactosyl-maltose + beta-cyclodextrin analysis of reaction conditions and structural identification of products Klebsiella aerogenes (Gal-G2)-beta-cyclodextrin + (Gal-G2)2-beta-cyclodextrin - r 372110 3.2.1.41 6-O-alpha-maltosyl cyclodextrin + H2O similar enzyme Saccharomyces cerevisiae glucose + cyclodextrin - ? 94364 3.2.1.41 6-O-alpha-maltosyl cyclodextrin + H2O similar enzyme Oryctolagus cuniculus glucose + cyclodextrin - ? 94364 3.2.1.41 6-O-alpha-maltotriosyl cyclomaltoheptaose + H2O - Oryctolagus cuniculus glucose + cyclodextrin - ? 94365 3.2.1.41 6-O-alpha-maltotriosyl cyclomaltoheptaose + H2O similar enzyme Saccharomyces cerevisiae glucose + cyclodextrin - ? 94365 3.2.1.41 6-O-alpha-maltotriosyl cyclomaltohexaose + H2O similar enzyme Saccharomyces cerevisiae glucose + cyclodextrin - ? 94366 3.2.1.41 6-O-alpha-maltotriosyl cyclomaltohexaose + H2O similar enzyme Oryctolagus cuniculus glucose + cyclodextrin - ? 94366 3.2.1.41 alpha-cyclodextrin + H2O - Thermofilum pendens ? - ? 381389 3.2.1.41 amylopectin + H2O - Thermus aquaticus maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O - Beta vulgaris maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O - Geobacillus stearothermophilus maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O - Zea mays maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O - Avena sativa maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O - Klebsiella aerogenes maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O - Thermoanaerobacterium thermosaccharolyticum maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O - Bacillus cereus maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O - Klebsiella pneumoniae maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O - Bacillus sp. (in: Bacteria) maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O - Niallia circulans maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O - Paenibacillus macerans maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O - Thermoanaerobacter thermohydrosulfuricus maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O - Oryza sativa maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O - Micrococcus sp. maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O - Pyrococcus woesei maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O - Bacteroides thetaiotaomicron maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O - Thermoanaerobacter ethanolicus maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O - Laceyella sacchari maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O - Thermoanaerobacter brockii subsp. finnii maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O - Thermoanaerobacter acetoethylicus maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O - Bacillus acidopullulyticus maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O - Thermoanaerobium sp. maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O - Thermus caldophilus maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O - Fervidobacterium pennivorans maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O - Oryza sativa maltooligosaccharides + maltose 34.1% of the rate with pullulan ? 94356 3.2.1.41 amylopectin + H2O branched polysaccharides Thermus aquaticus maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O branched polysaccharides Beta vulgaris maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O branched polysaccharides Geobacillus stearothermophilus maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O branched polysaccharides Zea mays maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O branched polysaccharides Avena sativa maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O branched polysaccharides Klebsiella aerogenes maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O branched polysaccharides Thermoanaerobacterium thermosaccharolyticum maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O branched polysaccharides Bacillus cereus maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O branched polysaccharides Klebsiella pneumoniae maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O branched polysaccharides Bacillus sp. (in: Bacteria) maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O branched polysaccharides Niallia circulans maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O branched polysaccharides Paenibacillus macerans maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O branched polysaccharides Thermoanaerobacter thermohydrosulfuricus maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O branched polysaccharides Oryza sativa maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O branched polysaccharides Micrococcus sp. maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O branched polysaccharides Pyrococcus woesei maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O branched polysaccharides Bacteroides thetaiotaomicron maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O branched polysaccharides Thermoanaerobacter ethanolicus maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O branched polysaccharides Laceyella sacchari maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O branched polysaccharides Thermoanaerobacter brockii subsp. finnii maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O branched polysaccharides Thermoanaerobacter acetoethylicus maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O branched polysaccharides Bacillus acidopullulyticus maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O branched polysaccharides Thermoanaerobium sp. maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O branched polysaccharides Thermus caldophilus maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O branched polysaccharides Fervidobacterium pennivorans maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O - Klebsiella pneumoniae UNF5023 maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O branched polysaccharides Klebsiella pneumoniae UNF5023 maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O - Thermoanaerobacter thermohydrosulfuricus E 101-69 maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O branched polysaccharides Thermoanaerobacter thermohydrosulfuricus E 101-69 maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O - Fervidobacterium pennivorans Ven5 maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O branched polysaccharides Fervidobacterium pennivorans Ven5 maltooligosaccharides + maltose - ? 94356 3.2.1.41 amylopectin + H2O - Thermotoga neapolitana ? - ? 94698 3.2.1.41 amylopectin + H2O - Pyrococcus furiosus ? - ? 94698 3.2.1.41 amylopectin + H2O 20% of activity compared to pullulan Zea mays ? - ? 94698 3.2.1.41 amylopectin + H2O 35% of activity compared to pullulan Desulfurococcus mucosus ? - ? 94698 3.2.1.41 amylopectin + H2O the enzyme prefers to debranch the DP6-12 side chains of amylopectin at pH 4.5 and 100°C Thermofilum pendens ? - ? 94698 3.2.1.41 amylopectin + H2O 12% of the activity compared to pullulan Paenibacillus barengoltzii ? - ? 94698 3.2.1.41 amylopectin + H2O hydrolyzed at 60% compared to pullulan unidentified ? - ? 94698 3.2.1.41 amylopectin + H2O the enzyme specifically attacks alpha-1,6-linkages of branched oligosaccharides Geobacillus thermocatenulatus ? - ? 94698 3.2.1.41 amylopectin + H2O the enzyme prefers to debranch the DP6-12 side chains of amylopectin at pH 4.5 and 100°C Thermofilum pendens DSM 2475 ? - ? 94698 3.2.1.41 amylopectin + H2O the enzyme specifically attacks alpha-1,6-linkages of branched oligosaccharides Geobacillus thermocatenulatus DSMZ730 ? - ? 94698 3.2.1.41 amylopectin + H2O - Anaerobranca gottschalkii maltotriose - ? 361118 3.2.1.41 amylopectin + H2O - Thermoanaerobacter ethanolicus malto-2-4-oligosaccharide + ? - ? 372377 3.2.1.41 amylopectin + H2O - Thermoanaerobacterium saccharolyticum maltotriose + maltose + ? - ? 416571 3.2.1.41 amylopectin + H2O - Thermoanaerobacterium saccharolyticum NTOU1 maltotriose + maltose + ? - ? 416571 3.2.1.41 amylopectin + H2O the enzyme can degrade both the alpha-1,4 and alpha-1,6-linkages of alpha-glucans Sulfolobus acidocaldarius D-glucose + maltose + maltotriose - ? 431592 3.2.1.41 amylopectin + H2O the enzyme can degrade both the alpha-1,4 and alpha-1,6-linkages of alpha-glucans Sulfolobus acidocaldarius DSM 639 D-glucose + maltose + maltotriose - ? 431592 3.2.1.41 amylopectin + H2O 6.0% of the activity with pullulan Exiguobacterium acetylicum maltotriose + maltose + maltooligosaccharides - ? 437018 3.2.1.41 amylopectin + H2O 6.0% of the activity with pullulan Exiguobacterium acetylicum YH5 maltotriose + maltose + maltooligosaccharides - ? 437018 3.2.1.41 amylopectin + H2O - Pyrobaculum calidifontis maltohexaose + maltopentaose + maltotetraose + maltotriose + maltose + D-glucose - ? 446994 3.2.1.41 amylopectin + H2O - Pyrobaculum calidifontis VA1 maltohexaose + maltopentaose + maltotetraose + maltotriose + maltose + D-glucose - ? 446994 3.2.1.41 amylopectin + H2O 28% of the activity compared to pullulan, the enzyme hydrolyzes amylopectin to release mainly maltotriose, maltotetraose, maltopentaose and maltohexaose, and their contents reach to 0.0044, 0.0068, 0.0073, 0.025 and 0.035 mg/ml, respectively, after 2 h incubation Priestia megaterium maltotriose + maltotetraose + maltopentaose + maltohexaose - ? 446996 3.2.1.41 amylopectin + H2O 28% of the activity compared to pullulan, the enzyme hydrolyzes amylopectin to release mainly maltotriose, maltotetraose, maltopentaose and maltohexaose, and their contents reach to 0.0044, 0.0068, 0.0073, 0.025 and 0.035 mg/ml, respectively, after 2 h incubation Priestia megaterium WW1210 maltotriose + maltotetraose + maltopentaose + maltohexaose - ? 446996 3.2.1.41 amylopectin beta-limit dextrin + H2O - Paenibacillus macerans maltotriose + maltose - ? 94359 3.2.1.41 amylopectin beta-limit dextrin + H2O - Laceyella sacchari maltotriose + maltose - ? 94359 3.2.1.41 amylose + H2O - Niallia circulans maltooligosaccharides + maltose - ? 94357 3.2.1.41 amylose + H2O - Thermoanaerobacter thermohydrosulfuricus maltooligosaccharides + maltose - ? 94357 3.2.1.41 amylose + H2O - Thermoanaerobium sp. maltooligosaccharides + maltose - ? 94357 3.2.1.41 amylose + H2O - Fervidobacterium pennivorans maltooligosaccharides + maltose - ? 94357 3.2.1.41 amylose + H2O weakly Geobacillus stearothermophilus maltooligosaccharides + maltose - ? 94357 3.2.1.41 amylose + H2O - Thermoanaerobacter thermohydrosulfuricus E 101-69 maltooligosaccharides + maltose - ? 94357 3.2.1.41 amylose + H2O - Fervidobacterium pennivorans Ven5 maltooligosaccharides + maltose - ? 94357 3.2.1.41 amylose + H2O - Pyrococcus furiosus ? - ? 94440 3.2.1.41 amylose + H2O - Thermofilum pendens ? - ? 94440 3.2.1.41 amylose + H2O - Anoxybacillus sp. WB42 ? - ? 94440 3.2.1.41 amylose + H2O 77% of activity compared to pullulan Desulfurococcus mucosus ? - ? 94440 3.2.1.41 amylose + H2O - Thermofilum pendens DSM 2475 ? - ? 94440 3.2.1.41 amylose + H2O - Thermoanaerobacter ethanolicus malto-2-4-oligosaccharide + ? - ? 372383 3.2.1.41 amylose + H2O - Lactococcus lactis maltose + maltotriose - ? 372385 3.2.1.41 amylose + H2O - Lactococcus lactis IBB500 maltose + maltotriose - ? 372385 3.2.1.41 amylose + H2O - Thermoanaerobacterium saccharolyticum maltotriose + maltose - ? 416573 3.2.1.41 amylose + H2O - Thermoanaerobacterium saccharolyticum NTOU1 maltotriose + maltose - ? 416573 3.2.1.41 beta-cyclodextrin + H2O 21% of activity compared to pullulan Desulfurococcus mucosus ? - ? 94205 3.2.1.41 beta-cyclodextrin + H2O in the initial stages, the ring-opening reactions of 6-O-glucosyl-beta-cyclodextrin, 6-O-maltosyl-beta-cyclodextrin and the debranching reactions of 6-O-maltooctaosyl-beta-cyclodextrin are firstly catalyzed. In the subsequent reactions, a serial of maltooligosaccharides are produced Thermofilum pendens ? - ? 94205 3.2.1.41 beta-cyclodextrin + H2O in the initial stages, the ring-opening reactions of 6-O-glucosyl-beta-cyclodextrin, 6-O-maltosyl-beta-cyclodextrin and the debranching reactions of 6-O-maltooctaosyl-beta-cyclodextrin are firstly catalyzed. In the subsequent reactions, a serial of maltooligosaccharides are produced Thermofilum pendens DSM 2475 ? - ? 94205 3.2.1.41 beta-limit dextrin + H2O - Zea mays ? - ? 259724 3.2.1.41 beta-limit dextrin + H2O - Oryza sativa ? 19.5% of the rate with pullulan ? 259724 3.2.1.41 beta-limit dextrin + H2O 135% of activity compared to pullulan Zea mays ? - ? 259724 3.2.1.41 beta-limited dextrin + H2O 19.7% of the activity with pullulan Exiguobacterium acetylicum maltotriose + maltose + maltooligosaccharides - ? 437224 3.2.1.41 beta-limited dextrin + H2O 19.7% of the activity with pullulan Exiguobacterium acetylicum YH5 maltotriose + maltose + maltooligosaccharides - ? 437224 3.2.1.41 cyclomaltooctaose + H2O gamma-cyclodextrin Klebsiella aerogenes 6,6-di-O-alpha-maltosyl-cG8 - ? 94367 3.2.1.41 dextran + H2O - Bacillus acidopullulyticus ? - ? 94189 3.2.1.41 dextrin + H2O - Geobacillus stearothermophilus maltooligosaccharides + maltose - ? 94362 3.2.1.41 dextrin + H2O - Bacillus sp. (in: Bacteria) maltooligosaccharides + maltose - ? 94362 3.2.1.41 dextrin + H2O - Pyrococcus woesei maltooligosaccharides + maltose - ? 94362 3.2.1.41 dextrin + H2O - Thermus caldophilus maltooligosaccharides + maltose - ? 94362 3.2.1.41 dextrin + H2O - Fervidobacterium pennivorans maltooligosaccharides + maltose - ? 94362 3.2.1.41 gamma-cyclodextrin + H2O 71% of activity compared to pullulan Desulfurococcus mucosus ? - ? 361127 3.2.1.41 gamma-cyclodextrin + H2O in the initial stages, the ring-opening reactions of gamma-cyclodextrin is firstly catalyzed. In the subsequent reactions, a serial of maltooligosaccharides are produced Thermofilum pendens ? - ? 361127 3.2.1.41 glycogen + H2O - Geobacillus stearothermophilus maltooligosaccharides + maltose - ? 94361 3.2.1.41 glycogen + H2O - Bacillus sp. (in: Bacteria) maltooligosaccharides + maltose - ? 94361 3.2.1.41 glycogen + H2O - Niallia circulans maltooligosaccharides + maltose - ? 94361 3.2.1.41 glycogen + H2O - Paenibacillus macerans maltooligosaccharides + maltose - ? 94361 3.2.1.41 glycogen + H2O - Thermoanaerobacter thermohydrosulfuricus maltooligosaccharides + maltose - ? 94361 3.2.1.41 glycogen + H2O - Micrococcus sp. maltooligosaccharides + maltose - ? 94361 3.2.1.41 glycogen + H2O - Thermus caldophilus maltooligosaccharides + maltose - ? 94361 3.2.1.41 glycogen + H2O - Fervidobacterium pennivorans maltooligosaccharides + maltose - ? 94361 3.2.1.41 glycogen + H2O - Oryza sativa maltooligosaccharides + maltose 6.3% of the rate with pullulan ? 94361 3.2.1.41 glycogen + H2O - Fervidobacterium pennivorans Ven5 maltooligosaccharides + maltose - ? 94361 3.2.1.41 glycogen + H2O - Thermotoga neapolitana ? - ? 94449 3.2.1.41 glycogen + H2O - Pyrococcus furiosus ? - ? 94449 3.2.1.41 glycogen + H2O hydrolyzed slowly. Hydrolyzed in an endo fashion to form a series of oligosaccharides as small as glucose, with the majority of product in the DP4 to DP6 range Pyrococcus furiosus ? - ? 94449 3.2.1.41 glycogen + H2O hydrolyzed slowly. Hydrolyzed in an endo fashion to form a series of oligosaccharides as small as glucose, with the majority of product in the DP4 to DP6 range Thermococcus litoralis ? - ? 94449 3.2.1.41 glycogen + H2O the enzyme can degrade both alpha-1,4 and alpha-1,6-linkages of alpha-glucan Sulfolobus acidocaldarius ? - ? 94449 3.2.1.41 glycogen + H2O hydrolyzed at 30% compared to pullulan unidentified ? - ? 94449 3.2.1.41 glycogen + H2O the enzyme can degrade both alpha-1,4 and alpha-1,6-linkages of alpha-glucan Sulfolobus acidocaldarius DSM 639 ? - ? 94449 3.2.1.41 glycogen + H2O hydrolyzed slowly. Hydrolyzed in an endo fashion to form a series of oligosaccharides as small as glucose, with the majority of product in the DP4 to DP6 range Thermococcus litoralis DSM 5473 ? - ? 94449 3.2.1.41 glycogen + H2O - Thermoanaerobacter ethanolicus malto-2-4-oligosaccharide + ? - ? 373328 3.2.1.41 glycogen + H2O - Thermoanaerobacterium saccharolyticum maltotriose + maltose - ? 417059 3.2.1.41 glycogen + H2O 6.1% of the activity with pullulan Exiguobacterium acetylicum maltotriose + maltose + maltooligosaccharides - ? 437580 3.2.1.41 glycogen + H2O 6.1% of the activity with pullulan Exiguobacterium acetylicum YH5 maltotriose + maltose + maltooligosaccharides - ? 437580 3.2.1.41 glycogen beta-limit dextrin + H2O - Beta vulgaris maltotriose + maltose + maltosetetraose - ? 94360 3.2.1.41 glycogen beta-limit dextrin + H2O - Avena sativa maltotriose + maltose + maltosetetraose - ? 94360 3.2.1.41 glycogen beta-limit dextrin + H2O - Bacillus cereus maltotriose + maltose + maltosetetraose - ? 94360 3.2.1.41 glycogen beta-limit dextrin + H2O - Bacillus sp. (in: Bacteria) maltotriose + maltose + maltosetetraose - ? 94360 3.2.1.41 glycogen beta-limit dextrin + H2O - Paenibacillus macerans maltotriose + maltose + maltosetetraose - ? 94360 3.2.1.41 glycogen beta-limit dextrin + H2O - Thermoanaerobacter thermohydrosulfuricus maltotriose + maltose + maltosetetraose - ? 94360 3.2.1.41 glycogen beta-limit dextrin + H2O - Oryza sativa maltotriose + maltose + maltosetetraose - ? 94360 3.2.1.41 glycogen beta-limit dextrin + H2O - Laceyella sacchari maltotriose + maltose + maltosetetraose - ? 94360 3.2.1.41 glycogen beta-limit dextrin + H2O - Bacillus acidopullulyticus maltotriose + maltose + maltosetetraose - ? 94360 3.2.1.41 guar galactomannan + H2O - Bacillus acidopullulyticus ? - ? 405396 3.2.1.41 maltoheptaose + H2O - Geobacillus stearothermophilus maltotriose + maltose + D-glucose - ? 94368 3.2.1.41 maltoheptaose + H2O - Thermoanaerobacter thermohydrosulfuricus maltotriose + maltose + D-glucose - ? 94368 3.2.1.41 maltohexaose + H2O - Geobacillus stearothermophilus maltotriose + maltose + D-glucose + maltotetraose - ? 94369 3.2.1.41 maltohexaose + H2O - Niallia circulans maltotriose + maltose + D-glucose + maltotetraose - ? 94369 3.2.1.41 maltohexaose + H2O - Thermoanaerobacter thermohydrosulfuricus maltotriose + maltose + D-glucose + maltotetraose - ? 94369 3.2.1.41 maltopentaose + H2O - Geobacillus stearothermophilus maltotriose + maltose - ? 94370 3.2.1.41 maltopentaose + H2O - Niallia circulans maltotriose + maltose - ? 94370 3.2.1.41 maltopentaose + H2O - Thermoanaerobacter thermohydrosulfuricus maltotriose + maltose - ? 94370 3.2.1.41 maltose + cG8 through the reversed action Klebsiella aerogenes 6-O-alpha-maltosyl-cG8 - r 94363 3.2.1.41 maltotetraose + H2O - Geobacillus stearothermophilus maltose + glucose + maltotriose - ? 94358 3.2.1.41 maltotetraose + H2O - Niallia circulans maltose + glucose + maltotriose - ? 94358 3.2.1.41 maltotetraose + H2O - Thermoanaerobacter thermohydrosulfuricus maltose + glucose + maltotriose - ? 94358 3.2.1.41 maltotetraose + H2O slowly Thermoanaerobium sp. maltose + glucose + maltotriose - ? 94358 3.2.1.41 metroxylon sagu + H2O native sago starch additional information debranched starch - ? 392648 3.2.1.41 additional information enzyme requires at least two glucose units in linear chain to be released by cleavage of an alpha-1,6-linkage Zea mays ? - ? 89 3.2.1.41 additional information no substrate: amylose Anaerobranca gottschalkii ? - ? 89 3.2.1.41 additional information no substrate: phytogen Zea mays ? - ? 89 3.2.1.41 additional information PulA knock-out strains display decreased binding to epithelial cells Streptococcus pyogenes ? - ? 89 3.2.1.41 additional information enzyme exhibits alpha-glucosyltransfer activity and produces an alpha-(1-6) linked compound of two maltotriose molecules from pullulan Oryza sativa ? - ? 89 3.2.1.41 additional information NPDE exhibits a unique catalytic preference for longer malto-oligosaccharides with more than 8 monomers, performing hydrolysis without the transgylcosylation or CD-hydrolyzing activities of other GH-13 enzymes Nostoc punctiforme ? - ? 89 3.2.1.41 additional information pullulanase is a debranching enzyme which hydrolyses the alpha-1,6-glucosidic linkages in pullulan and other amylaceous polysaccharides Bacillus acidopullulyticus ? - ? 89 3.2.1.41 additional information hydrolysis of freeze-dried waxy maize starch nanocrystals by pullulanase isoamylase as debranching enzymes and by beta-amylase, cleavage profile, overview Raoultella planticola ? - ? 89 3.2.1.41 additional information NPDE hydrolyzes both alpha-1,4- and alpha-1,6-glucosidic linkages with a substrate specificity in descending order of pullulan, amylopectin, starch, amylose. NPDE has no specificity for alpha-, beta-, and gamma-cyclodextris, and hydrolyzes alpha-1,6-glucosidic linkages more rapidly than alpha-1,4-glucosidic linkages. NPDE hydrolyzes the alpha-1,6 glycosidic linkages of malto-oligosaccharides, molecular basis for the substrate specificity and the catalytic properties, overview Nostoc punctiforme ? - ? 89 3.2.1.41 additional information pullulanase effectively hydrolyzes pullulan, soluble starch and dextran Bacillus acidopullulyticus ? - ? 89 3.2.1.41 additional information the BaPul13A active centre in which hydrolysis of alpha-1,6 linkages occurs with net retention of anomeric configuration, via a covalent glycosyl-enzyme intermediate. Complete starch hydrolysis requires a consortium of enzymes including endo-amylases, glucoamylases and alpha-glucosidases as well as diverse alpha-1,6 cleaving enzymes including pullulanases Bacillus acidopullulyticus ? - ? 89 3.2.1.41 additional information the enzyme hydrolyzes alpha-1,6-glucosidic linkages in polysacchrides Bacillus acidopullulyticus ? - ? 89 3.2.1.41 additional information with glycogen and branched beta-cyclodextrins as substrates, pullulanase shows high-level specificity for the hydrolysis of the outer side chains of glycogen with three to five glucosyl residues. Debranching of the outer side chain of glycogen by pullulanase AmyX Bacillus subtilis ? - ? 89 3.2.1.41 additional information amylopullulanases (type II pullulanases) are able to degrade both the alpha-1,6 and alpha-1,4 glucosidic bonds of starch Thermococcus siculi ? - ? 89 3.2.1.41 additional information no substrate: amylose. Enzyme displays transglycosylation activity, transferring the maltotriosyl residue of pullulan to aesculin by forming alpha-1,6-glucosidic linkages Thermotoga neapolitana ? - ? 89 3.2.1.41 additional information the enzyme cleaves both alpha-1,4- and alpha-1,6-glycosidic linkages in starch, pullulan, amylopectin, and other related oligosaccharides Thermoanaerobacterium saccharolyticum ? - ? 89 3.2.1.41 additional information hydrolyzes the alpha-1,6 linkage in pullulan, alpha-1,4 linkages in amylose and soluble starch. No activity against maltohexaose or other smaller alpha-1,4-linked oligosaccharides Pyrococcus furiosus ? - ? 89 3.2.1.41 additional information hydrolyzes the alpha-1,6 linkage in pullulan, alpha-1,4 linkages in amylose and soluble starch. No activity against maltohexaose or other smaller alpha-1,4-linked oligosaccharides Thermococcus litoralis ? - ? 89 3.2.1.41 additional information the enzyme also hydrolyzes oligosaccharides but much slower. The longer the oligosaccharide chain, the higher the hydrolysis rate Pyrococcus furiosus ? - ? 89 3.2.1.41 additional information the enzyme can degrade both alpha-1,4 and alpha-1,6-linkages of alpha-glucan Sulfolobus acidocaldarius ? - ? 89 3.2.1.41 additional information negligible activity on wheat flour and maltodextrin, no activity on amylose Exiguobacterium sp. ? - ? 89 3.2.1.41 additional information no substrates: maltotriose, maltose Exiguobacterium acetylicum ? - ? 89 3.2.1.41 additional information the enzyme preferably debranches long branches at alpha-1,6 glycosidic bonds of starch, producing amylose, linear or branched oligosaccharides, but is nonreactive against short branches. In addition, the enzyme acts as limit dextrinase, reaction of EC 3.2.1.142, releasing maltotriose, maltotetraose and maltopentaose from limit dextrin Anoxybacillus sp. ? - ? 89 3.2.1.41 additional information the enzyme does not show activity towards amylose, maltoheptaose, maltohexaose, maltopentaose and maltotetraose Priestia megaterium ? - ? 89 3.2.1.41 additional information the enzyme has no activity toward alpha-1,4-glycosidic linkages Geobacillus thermocatenulatus ? - ? 89 3.2.1.41 additional information with glycogen and branched beta-cyclodextrins as substrates, pullulanase shows high-level specificity for the hydrolysis of the outer side chains of glycogen with three to five glucosyl residues. Debranching of the outer side chain of glycogen by pullulanase AmyX Bacillus subtilis 168 ? - ? 89 3.2.1.41 additional information the enzyme can degrade both alpha-1,4 and alpha-1,6-linkages of alpha-glucan Sulfolobus acidocaldarius DSM 639 ? - ? 89 3.2.1.41 additional information the enzyme cleaves both alpha-1,4- and alpha-1,6-glycosidic linkages in starch, pullulan, amylopectin, and other related oligosaccharides Thermoanaerobacterium saccharolyticum NTOU1 ? - ? 89 3.2.1.41 additional information the enzyme does not show activity towards amylose, maltoheptaose, maltohexaose, maltopentaose and maltotetraose Priestia megaterium WW1210 ? - ? 89 3.2.1.41 additional information amylopullulanases (type II pullulanases) are able to degrade both the alpha-1,6 and alpha-1,4 glucosidic bonds of starch Thermococcus siculi HJ21 ? - ? 89 3.2.1.41 additional information hydrolyzes the alpha-1,6 linkage in pullulan, alpha-1,4 linkages in amylose and soluble starch. No activity against maltohexaose or other smaller alpha-1,4-linked oligosaccharides Thermococcus litoralis DSM 5473 ? - ? 89 3.2.1.41 additional information the enzyme preferably debranches long branches at alpha-1,6 glycosidic bonds of starch, producing amylose, linear or branched oligosaccharides, but is nonreactive against short branches. In addition, the enzyme acts as limit dextrinase, reaction of EC 3.2.1.142, releasing maltotriose, maltotetraose and maltopentaose from limit dextrin Anoxybacillus sp. SK3-4 ? - ? 89 3.2.1.41 additional information the enzyme has no activity toward alpha-1,4-glycosidic linkages Geobacillus thermocatenulatus DSMZ730 ? - ? 89 3.2.1.41 additional information no substrates: maltotriose, maltose Exiguobacterium acetylicum YH5 ? - ? 89 3.2.1.41 phytoglycogen + H2O 9% of activity compared to pullulan Zea mays ? - ? 374355 3.2.1.41 potato starch + H2O 14% of the activity with pullulan Exiguobacterium sp. ? - ? 401777 3.2.1.41 pullulan + H2O - Pyrococcus furiosus ? - ? 94190 3.2.1.41 pullulan + H2O - Bacillus acidopullulyticus ? - ? 94190 3.2.1.41 pullulan + H2O - Zea mays ? - ? 94190 3.2.1.41 pullulan + H2O - Desulfurococcus mucosus ? - ? 94190 3.2.1.41 pullulan + H2O - Bacillus deramificans ? - ? 94190 3.2.1.41 pullulan + H2O - Thermococcus siculi ? - ? 94190 3.2.1.41 pullulan + H2O - Halorubrum sp. Ha25 ? - ? 94190 3.2.1.41 pullulan + H2O - Exiguobacterium sp. ? - ? 94190 3.2.1.41 pullulan + H2O - Pullulanibacillus naganoensis ? - ? 94190 3.2.1.41 pullulan + H2O - Thermofilum pendens ? - ? 94190 3.2.1.41 pullulan + H2O - Anoxybacillus sp. WB42 ? - ? 94190 3.2.1.41 pullulan + H2O - Fontibacillus sp. DSHK 107 ? - ? 94190 3.2.1.41 pullulan + H2O pullalan synthesized by Aureobasidium pullulans strain AP329 as exopolysaccharide and purified, development of a colorimetric assay method for pullalan quantification, overview Bacillus deramificans ? - ? 94190 3.2.1.41 pullulan + H2O substrate from Aureobasidium pullulans strain FB-1 Bacillus acidopullulyticus ? - ? 94190 3.2.1.41 pullulan + H2O - Thermococcus siculi HJ21 ? - ? 94190 3.2.1.41 pullulan + H2O - Thermofilum pendens DSM 2475 ? - ? 94190 3.2.1.41 pullulan + H2O - Thermus aquaticus maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Beta vulgaris maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Bacillus subtilis maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Geobacillus stearothermophilus maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Zea mays maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Avena sativa maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Pyrococcus furiosus maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Klebsiella aerogenes maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Thermoanaerobacterium thermosaccharolyticum maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Bacillus cereus maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Klebsiella pneumoniae maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Bacillus sp. (in: Bacteria) maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Niallia circulans maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Paenibacillus macerans maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Thermoanaerobacter thermohydrosulfuricus maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Oryza sativa maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Micrococcus sp. maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Pyrococcus woesei maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Aureobasidium pullulans maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Bacteroides thetaiotaomicron maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - unidentified maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Thermoanaerobacter ethanolicus maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Laceyella sacchari maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Thermoanaerobacter brockii subsp. finnii maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Thermoanaerobacter acetoethylicus maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Bacillus acidopullulyticus maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Thermoanaerobium sp. maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Thermus caldophilus maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Fervidobacterium pennivorans maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Thermotoga neapolitana maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Thermoanaerobacterium saccharolyticum maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Anoxybacillus sp. maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Geobacillus thermocatenulatus maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Anaerobranca gottschalkii maltotriose + ? final product ? 94354 3.2.1.41 pullulan + H2O - Exiguobacterium acetylicum maltotriose + ? complete conversion to maltotriose ? 94354 3.2.1.41 pullulan + H2O preferred substrate Oryza sativa maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O preparation of maltotriose by hydrolyzing of pullulan with pullulanase, overview Bacillus deramificans maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O specific for pullulan 1,6-glycosidic linkages Lactococcus lactis maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O higher specific activity against pullulan than against starch. Enzyme attacks only the alpha-1,6 linkages in pullulan Pyrococcus furiosus maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O higher specific activity against pullulan than against starch. Enzyme attacks only the alpha-1,6 linkages in pullulan Thermococcus litoralis maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O soluble enzyme is highly reactive towards pullulan but was unable to degrade short branches in starch Anoxybacillus ayderensis maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O the enzyme exhibits strict substrate specificity towards pullulan, but shows relatively low activity towards amylopectin and no activity towards other tested polysaccharides Paenibacillus barengoltzii maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O the enzyme hydrolyzes pullulan to yield mainly maltotriose, more than 95% Priestia megaterium maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Bacillus subtilis 168 maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Klebsiella pneumoniae UNF5023 maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Thermoanaerobacter thermohydrosulfuricus E 101-69 maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Thermoanaerobacterium saccharolyticum NTOU1 maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O soluble enzyme is highly reactive towards pullulan but was unable to degrade short branches in starch Anoxybacillus ayderensis DSM 28779 maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Fervidobacterium pennivorans Ven5 maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O the enzyme hydrolyzes pullulan to yield mainly maltotriose, more than 95% Priestia megaterium WW1210 maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O higher specific activity against pullulan than against starch. Enzyme attacks only the alpha-1,6 linkages in pullulan Thermococcus litoralis DSM 5473 maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Anoxybacillus sp. SK3-4 maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Geobacillus thermocatenulatus DSMZ730 maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O - Exiguobacterium acetylicum YH5 maltotriose + ? complete conversion to maltotriose ? 94354 3.2.1.41 pullulan + H2O specific for pullulan 1,6-glycosidic linkages Lactococcus lactis IBB500 maltotriose + ? - ? 94354 3.2.1.41 pullulan + H2O best substrate Thermoanaerobacter ethanolicus malto-2-4-oligosaccharide + ? - ? 374461 3.2.1.41 pullulan + H2O - additional information maltotriose + maltose - ? 393381 3.2.1.41 pullulan + H2O - Bacillus cereus maltotriose + maltose - ? 393381 3.2.1.41 pullulan + H2O - Bacillus sp. (in: Bacteria) maltotriose + maltose - ? 393381 3.2.1.41 pullulan + H2O - Geobacillus thermoleovorans maltotriose + maltose - ? 393381 3.2.1.41 pullulan + H2O - Pyrobaculum calidifontis maltotriose + maltose - ? 393381 3.2.1.41 pullulan + H2O - Bacillus sp. (in: Bacteria) AN-7 maltotriose + maltose - ? 393381 3.2.1.41 pullulan + H2O - Geobacillus thermoleovorans US105 maltotriose + maltose - ? 393381 3.2.1.41 pullulan + H2O - Bacillus cereus FDTA 13 maltotriose + maltose - ? 393381 3.2.1.41 pullulan + H2O - Pyrobaculum calidifontis VA1 maltotriose + maltose - ? 393381 3.2.1.41 pullulan + H2O this enzyme can attack alpha-1,6- and alpha-1,4-glycosidic linkages in pullulan, and produce a mixture of maltotriose, maltose and glucose Thermus thermophilus maltotriose + maltose + D-glucose - ? 445836 3.2.1.41 rice flour + H2O 15% of the activity with pullulan Exiguobacterium sp. ? - ? 434187 3.2.1.41 soluble starch - Beta vulgaris maltotriose + maltose - ? 94355 3.2.1.41 soluble starch - Klebsiella aerogenes maltotriose + maltose - ? 94355 3.2.1.41 soluble starch - Klebsiella pneumoniae maltotriose + maltose - ? 94355 3.2.1.41 soluble starch - Niallia circulans maltotriose + maltose - ? 94355 3.2.1.41 soluble starch - Thermoanaerobacter thermohydrosulfuricus maltotriose + maltose - ? 94355 3.2.1.41 soluble starch - Oryza sativa maltotriose + maltose - ? 94355 3.2.1.41 soluble starch - Pyrococcus woesei maltotriose + maltose - ? 94355 3.2.1.41 soluble starch - Bacillus acidopullulyticus maltotriose + maltose - ? 94355 3.2.1.41 soluble starch - Thermoanaerobium sp. maltotriose + maltose - ? 94355 3.2.1.41 soluble starch - Thermus caldophilus maltotriose + maltose - ? 94355 3.2.1.41 soluble starch - Fervidobacterium pennivorans maltotriose + maltose - ? 94355 3.2.1.41 soluble starch - Oryza sativa maltotriose + maltose 15.8% of the rate with pullulan ? 94355 3.2.1.41 soluble starch - Fervidobacterium pennivorans Ven5 maltotriose + maltose - ? 94355 3.2.1.41 soluble starch - Pyrococcus furiosus ? - ? 393521 3.2.1.41 soluble starch + H2O - Bacillus acidopullulyticus ? - ? 94576 3.2.1.41 soluble starch + H2O - Halorubrum sp. Ha25 ? - ? 94576 3.2.1.41 soluble starch + H2O 17% of the activity with pullulan Exiguobacterium sp. ? - ? 94576 3.2.1.41 soluble starch + H2O hydrolyzed at 80% compared to pullulan unidentified ? - ? 94576 3.2.1.41 soluble starch + H2O the enzyme specifically attacks alpha-1,6-linkages of branched oligosaccharides Geobacillus thermocatenulatus ? - ? 94576 3.2.1.41 soluble starch + H2O the enzyme specifically attacks alpha-1,6-linkages of branched oligosaccharides Geobacillus thermocatenulatus DSMZ730 ? - ? 94576 3.2.1.41 soluble starch + H2O - Thermoanaerobacter ethanolicus malto-2-4-oligosaccharide + ? - ? 374643 3.2.1.41 soluble starch + H2O - Pyrococcus furiosus maltotriose + maltose - ? 382822 3.2.1.41 soluble starch + H2O - Thermoanaerobacterium saccharolyticum maltotriose + maltose - ? 382822 3.2.1.41 soluble starch + H2O - Thermoanaerobacterium saccharolyticum NTOU1 maltotriose + maltose - ? 382822 3.2.1.41 soluble starch + H2O 12.8% of the activity with pullulan Exiguobacterium acetylicum maltotriose + maltose + maltooligosaccharides - ? 437906 3.2.1.41 starch + H2O - Thermotoga neapolitana ? - ? 94621 3.2.1.41 starch + H2O - Thermofilum pendens ? - ? 94621 3.2.1.41 starch + H2O 62% of activity compared to pullulan Desulfurococcus mucosus ? - ? 94621 3.2.1.41 starch + H2O together with amylase, both enzymes immobilized on calcium alginate beads Bacillus acidopullulyticus ? - ? 94621 3.2.1.41 starch + H2O higher specific activity against pullulan than against starch. Hydrolyzed in an endo fashion to form a series of oligosaccharides as small as glucose, with the majority of product in the DP4 to DP6 range Pyrococcus furiosus ? - ? 94621 3.2.1.41 starch + H2O higher specific activity against pullulan than against starch. Hydrolyzed in an endo fashion to form a series of oligosaccharides as small as glucose, with the majority of product in the DP4 to DP6 range Thermococcus litoralis ? - ? 94621 3.2.1.41 starch + H2O - Thermofilum pendens DSM 2475 ? - ? 94621 3.2.1.41 starch + H2O higher specific activity against pullulan than against starch. Hydrolyzed in an endo fashion to form a series of oligosaccharides as small as glucose, with the majority of product in the DP4 to DP6 range Thermococcus litoralis DSM 5473 ? - ? 94621 3.2.1.41 starch + H2O - Aureobasidium pullulans maltotriose + ? - ? 361117 3.2.1.41 starch + H2O - Anaerobranca gottschalkii maltotriose + ? - ? 361117 3.2.1.41 starch + H2O - Lactococcus lactis maltose + maltotriose - ? 371173 3.2.1.41 starch + H2O - Lactococcus lactis IBB500 maltose + maltotriose - ? 371173 3.2.1.41 starch + H2O the enzyme can degrade both the alpha-1,4 and alpha-1,6-linkages of alpha-glucans Sulfolobus acidocaldarius D-glucose + maltose + maltotriose - ? 401913 3.2.1.41 starch + H2O the enzyme can degrade both the alpha-1,4 and alpha-1,6-linkages of alpha-glucans Sulfolobus acidocaldarius DSM 639 D-glucose + maltose + maltotriose - ? 401913 3.2.1.41 starch + H2O - Pyrobaculum calidifontis maltohexaose + maltopentaose + maltotetraose + maltotriose + maltose + D-glucose - ? 448139 3.2.1.41 starch + H2O - Pyrobaculum calidifontis VA1 maltohexaose + maltopentaose + maltotetraose + maltotriose + maltose + D-glucose - ? 448139 3.2.1.41 starch + H2O following immobilization through covalent attachment to three epoxides (ReliZyme EP403/M, ImmobeadIB-150P, and Immobead IB-150A) and an amino-epoxide (ReliZyme HFA403/M), all PulASK derivatives are active on both short and long branches in starch producing reducing sugars (predominantly maltotriose) and oligosaccharides (chain lenght G8 and higher), respectively, a feature that is absent in the free enzyme. Individual or coimmobilization causes changes in the product specificity, presumably due to changes in the enzyme binding pocket caused by the influence of carrier surface properties (hydrophobicor hydrophilic) and the lengths of the spacer arms Anoxybacillus ayderensis maltotriose + oligosaccharides - ? 448140 3.2.1.41 starch + H2O following immobilization through covalent attachment to three epoxides (ReliZyme EP403/M, ImmobeadIB-150P, and Immobead IB-150A) and an amino-epoxide (ReliZyme HFA403/M), all PulASK derivatives are active on both short and long branches in starch producing reducing sugars (predominantly maltotriose) and oligosaccharides (chain lenght G8 and higher), respectively, a feature that is absent in the free enzyme. Individual or coimmobilization causes changes in the product specificity, presumably due to changes in the enzyme binding pocket caused by the influence of carrier surface properties (hydrophobicor hydrophilic) and the lengths of the spacer arms Anoxybacillus ayderensis DSM 28779 maltotriose + oligosaccharides - ? 448140