3.2.1.119	(R)-amygdalin + H2O	reaction 16% of the rate of vicianine hydrolysis	Davallia trichomanoides	mandelonitrile + beta-gentiobiose	-	?	361149	
3.2.1.119	(R)-amygdalin + H2O	reaction 27% of the rate of vicianine hydrolysis	Davallia trichomanoides	mandelonitrile + beta-gentiobiose	-	?	361149	
3.2.1.119	(R)-amygdalin + H2O	-	Davallia trichomanoides	(R)-mandelonitrile + beta-gentiobiose	-	?	422219	
3.2.1.119	(R)-prunasin + H2O	reaction 12% of the rate of vicianine hydrolysis	Davallia trichomanoides	mandelonitrile + D-glucose	-	?	361150	
3.2.1.119	(R)-prunasin + H2O	reaction 14% of the rate of vicianine hydrolysis	Davallia trichomanoides	mandelonitrile + D-glucose	-	?	361150	
3.2.1.119	(R)-vicianin + H2O	-	Davallia trichomanoides	mandelonitrile + vicianose	-	?	361148	
3.2.1.119	(R)-vicianin + H2O	i.e. beta-vicianoside of (R)-mandelonitrile, strict requirement for (R)-epimers	Davallia trichomanoides	mandelonitrile + vicianose	-	?	361148	
3.2.1.119	(R)-vicianin + H2O	cyanogenic disaccharides preferred over monosaccharides	Davallia trichomanoides	mandelonitrile + vicianose	-	?	361148	
3.2.1.119	(R)-vicianin + H2O	involved in catabolism of cyanogenic glycosides	Davallia trichomanoides	mandelonitrile + vicianose	-	?	361148	
3.2.1.119	2-nitrophenyl beta-D-glucopyranoside + H2O	poor substrate	Davallia trichomanoides	2-nitrophenol + D-glucopyranose	-	?	214437	
3.2.1.119	2-nitrophenyl beta-D-xylopyranoside + H2O	poor substrate	Davallia trichomanoides	2-nitrophenol + D-xylopyranose	-	?	361154	
3.2.1.119	2-phenylethyl beta-primeveroside + H2O	-	Vicia sativa subsp. nigra	?	-	?	390667	
3.2.1.119	4-nitrophenyl beta-D-glucopyranoside + H2O	poor substrate	Davallia trichomanoides	4-nitrophenol + D-glucopyranose	-	?	94269	
3.2.1.119	furcatin + H2O	recombinant enzyme expressed in Escherichia coli does not hydrolyze furcatin	Vicia sativa subsp. nigra	?	-	?	392007	
3.2.1.119	additional information	no substrates are lactose, o-nitrophenyl-beta-D-galactopyranoside, beta-methyl-D-glucoside, beta-gentiobiose, linamarin, cellobiose, p-nitrophenyl-alpha-D-glucopyranoside, salicin, phenyl-beta-D-glucoside, maltose and sucrose	Davallia trichomanoides	?	-	?	89	
3.2.1.119	additional information	does not hydrolyze amygdalin, mandelonitrile-, 2-phenylethyl-, p-nitrophenyl beta-gentiobioside, p-nitrophenyl beta-D-xylopyranoside, p-nitrophenyl alpha-L-arabinopyranoside and p-nitrophenyl beta-D-galactopyranoside	Vicia sativa subsp. nigra	?	-	?	89	
3.2.1.119	p-nitrophenyl beta-primeveroside + H2O	recombinant enzyme expressed in Escherichia coli does not hydrolyze p-nitrophenyl beta-primeveroside	Vicia sativa subsp. nigra	?	-	?	393089	
3.2.1.119	p-nitrophenyl-beta-D-xylopyranoside + H2O	poor substrate	Davallia trichomanoides	4-nitrophenol + beta-D-xylopyranose	-	?	189714	
3.2.1.119	prunasin + H2O	shows 34% of its vicianin hydrolase activity with prunasin	Vicia sativa subsp. nigra	D-mandelonitrile + beta-D-glucose	-	?	94662	
3.2.1.119	vicianin + H2O	best substrate, recombinant protein shows the same pattern of substrate specificity as the native enzyme	Vicia sativa subsp. nigra	vicianose + D-mandelonitrile	-	?	393819