3.1.21.3 3'-biotinylated 51 bp dsDNA + H2O - Micrococcus luteus ? - ? 364429 3.1.21.3 5'-biotinylated 51 bp ssDNA + H2O - Micrococcus luteus ? - ? 364430 3.1.21.3 DNA + H2O - Escherichia coli K-12 ? - ? 141228 3.1.21.3 DNA + H2O - synthetic construct ? - ? 141228 3.1.21.3 DNA + H2O - Geobacillus stearothermophilus ? - ? 141228 3.1.21.3 DNA + H2O - Vibrio vulnificus YJ016 ? - ? 141228 3.1.21.3 DNA + H2O EcoR124I couples ATP hydrolysis to bidirectional DNA translocation Escherichia coli ? - ? 141228 3.1.21.3 DNA + H2O the enzyme must overcome a similar slow step before translocation reaches a steady state Escherichia coli ? - ? 141228 3.1.21.3 DNA + H2O R.HpyAXII effectively restricts chromosomal DNA during natural transformation Helicobacter pylori ? - ? 141228 3.1.21.3 DNA + H2O HsdR subunit can produce only a single cleavage of the phosphodiester backbone of the DNA but can cooperate with another HsdR subunit to produce full DNA cleavage, producing DNA with overhanging ends of single-stranded DNA. On a single-site plasmid, cleavage requires the association of HsdR, from solution, to the cleavage complex in order to produce double-strand cleavage Escherichia coli ? - ? 141228 3.1.21.3 DNA + H2O the PspGI restriction-modification system recognizes the sequence CCWGG. R.PspGI cuts DNA before the first C in the cognate sequence and M.PspGI methylates N4 of one of the cytosines in the sequence. R.PspGI flips both bases in the central base pair out of the duplex Pyrococcus sp. ? - ? 141228 3.1.21.3 DNA + H2O R.HpyAXII effectively restricts chromosomal DNA during natural transformation Helicobacter pylori NSH57 ? - ? 141228 3.1.21.3 DNA + H2O - Geobacillus stearothermophilus CPW11 ? - ? 141228 3.1.21.3 duplex DNA + ATP - Salmonella enterica subsp. enterica serovar Typhimurium double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate - ? 93074 3.1.21.3 duplex DNA + ATP - Staphylococcus aureus double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate - ? 93074 3.1.21.3 duplex DNA + ATP - Escherichia coli double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate - ? 93074 3.1.21.3 duplex DNA + ATP - Citrobacter freundii double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate - ? 93074 3.1.21.3 duplex DNA + ATP overview of recognition sequences Escherichia coli double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate - ? 93074 3.1.21.3 duplex DNA + ATP overview of recognition sequences Citrobacter freundii double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate - ? 93074 3.1.21.3 duplex DNA + ATP the wild type enzyme EcoK cleaves circular DNA. Only one endonuclease molecule is required per cleavage event. Cleavage of linear DNA may require a second endonuclease molecule Escherichia coli double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate - ? 93074 3.1.21.3 duplex DNA + ATP restricts unmodified phage DNA Staphylococcus aureus double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate - ? 93074 3.1.21.3 duplex DNA + ATP the enzyme is both a restriction endonuclease and a modification methylase. Hemi-methylated DNA is the preferred substrate for methylation Escherichia coli double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate - ? 93074 3.1.21.3 duplex DNA + ATP the site of restriction cleavage is random, occuring between 1 and 5 kb from the recognition site. The modification methylase acts directly at the recognition sequence Escherichia coli double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate - ? 93074 3.1.21.3 duplex DNA + ATP the modification methylase binds sequence specifically to DNA and protects a 25bp fragment containing its cognate recognition sequence from digestion by exonuclease III, specific adenine on each strand of DNA is the site of methylation Escherichia coli double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate - ? 93074 3.1.21.3 duplex DNA + ATP supercoiled with one or two SR124I recognition sites is cleaved by the same mechanism. Nicked-circle DNA is an intermediate of the cleavage reaction Escherichia coli double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate - ? 93074 3.1.21.3 duplex DNA + ATP EcoAI preferentially generates 3'-overhangs of 2-3 nt. Displays some preference for the formation of 5'-overhangs of a length of 6-7 and 3-5 nt, respectively. type I restriction enzymes require two restriction subunits to introduce DNA double-stran breaks, each providing one catalytic center for phosphodiester bond hydrolysis Escherichia coli double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate - ? 93074 3.1.21.3 duplex DNA + ATP EcoKI displays some preference for the formation of 5'-overhangs of a length of 6-7 and 3-5 nt, respectively. Type I restriction enzymes require two restriction subunits to introduce DNA double-stran breaks, each providing one catalytic center for phosphodiester bond hydrolysis Escherichia coli double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate - ? 93074 3.1.21.3 duplex DNA + ATP EcoR124I displays some preference for the formation of 5'-overhangs of a length of 6-7 and 3-5 nt, respectively. Type I restriction enzymes require two restriction subunits to introduce DNA double-stran breaks, each providing one catalytic center for phosphodiester bond hydrolysis Escherichia coli double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate - ? 93074 3.1.21.3 duplex DNA + ATP initiation of translocation by type I restriction-modification enzymes is associated with a short DNA extrusion Escherichia coli double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate - ? 93074 3.1.21.3 duplex DNA + ATP the two motor subunits of Eco124I are independent motors that translocate along the helical pitch of the DNA. Dynamic termination and reinitiation of translocation activity is governed by disassembly and reassembly of the enzyme Escherichia coli double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate - ? 93074 3.1.21.3 duplex DNA + ATP type I enzymes recognize bipartite DNA sequences comprising two half-sequences separated by a gap, for example, AACNNNNNNGTGC (AAC N6 GTGC) where N=any base Escherichia coli double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate - ? 93074 3.1.21.3 duplex DNA + ATP restricts unmodified phage DNA Staphylococcus aureus RN450 double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate - ? 93074 3.1.21.3 duplex DNA + ATP - Escherichia coli A0 34/86 (O83:K24:H31) double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate - ? 93074 3.1.21.3 duplex DNA + ATP - Vibrio vulnificus double-stranded DNA fragments with terminal 5'-phosphate + ADP + phosphate - ? 429250 3.1.21.3 linear plasmid DNA pDRM-2R + ATP - Escherichia coli ? - ? 429299 3.1.21.3 additional information - Escherichia coli ? - ? 89 3.1.21.3 additional information the database REBASE contains information about recognition sites and cleavage sites Escherichia coli ? - ? 89 3.1.21.3 additional information restricts unmodified phage DNA after both infection and transfection Staphylococcus aureus ? - ? 89 3.1.21.3 additional information a bacterial population may switch the recognition sequence of its type I restriction-modification system by single recombination events and thus is able to maintain a prokaryotic analogue of the immune system of variable specificity Escherichia coli ? - ? 89 3.1.21.3 additional information enzyme restricts the exchange of genetic material between bacteria of different strains or species Salmonella enterica subsp. enterica serovar Typhimurium ? - ? 89 3.1.21.3 additional information enzyme restricts the exchange of genetic material between bacteria of different strains or species Escherichia coli ? - ? 89 3.1.21.3 additional information isoform Sau1 is the major mechanism for blocking transfer of resistance genes and other mobile genetic elements into Staphylococcus aureus isolates from other species, as well as for controlling the spread of resistance genes between isolates of different Staphylococcus aureus lineages Staphylococcus aureus ? - ? 89 3.1.21.3 additional information basal transcription from promoter esp1396ICR results in gradual accumulation of C.Esp1396I, which upon dimerization binds to a single site in promoter esp1396IM, preventing further transcription from this promoter. Further accumulation of C.Esp1396I results in activation and then gradual repression of promoter esp1396ICR synthetic construct ? - ? 89 3.1.21.3 additional information C.PvuII binding to operator left activates transcription. Binding preference for operator left over operator right. All four bases of the inter-operator TGTA spacer are required for C.PvuII-dependent activation Escherichia coli K-12 ? - ? 89 3.1.21.3 additional information does not cut pUC19 plasmid Staphylococcus aureus ? - ? 89 3.1.21.3 additional information determination of target sequences recognized by the Sau1 Type I RM systems present in a wide range of the most prevalent Staphylococcus aureus lineages and assigned the sequences recognized to particular target recognition domains within the RM enzymes Staphylococcus aureus ? - ? 89 3.1.21.3 additional information restricts unmodified phage DNA after both infection and transfection Staphylococcus aureus RN450 ? - ? 89 3.1.21.3 plasmid DNA + H2O R.HpyAXII effectively restricts unmethylated plasmid during natural transformation Helicobacter pylori ? - ? 93087 3.1.21.3 plasmid DNA + H2O EcoKI prefers to have a partially filled DNA-binding site rather than one fully occupied by non-specific DNA. Dimerization of EcoKI does not occur before DNA binding and takes place on specific sites before any looping. Dimerization occurs before the two specific sites are bought together. Looping initially occurs between a target site and a non-specific region of DNA Escherichia coli ? - ? 93087 3.1.21.3 plasmid DNA + H2O R.HpyAXII effectively restricts unmethylated plasmid during natural transformation Helicobacter pylori NSH57 ? - ? 93087 3.1.21.3 plasmid pACYC184 + ATP - Staphylococcus aureus ? - ? 430619 3.1.21.3 plasmid pET20b + ATP - Staphylococcus aureus ? - ? 430620 3.1.21.3 plasmid pTK-neo + ATP the enzyme recognises the symmetrical sequence GAAN7TTC at position 2535 bp Escherichia coli ? - ? 430621 3.1.21.3 plasmid pUC19 + ATP the enzyme recognises the symmetrical sequence GAAN7TTC at positions 1126 bp and 2294 bp Escherichia coli ? - ? 430622 3.1.21.3 supercoiled plasmid DNA pRK + ATP - Escherichia coli ? - ? 429404 3.1.21.3 synthetic oligonucleotide + ATP recognition sequence: CA(underlined)C(5N)T(underlined)GGC Escherichia coli ? - ? 382860 3.1.21.3 synthetic oligonucleotide + ATP recognition sequence: GA(underlined)C(5N)RT(underlined)AAY Escherichia coli ? - ? 382860 3.1.21.3 synthetic oligonucleotide + ATP recognition sequence: GCA(underlined)(6N)CT(underlined)GA Escherichia coli ? - ? 382860 3.1.21.3 synthetic oligonucleotide + ATP recognition sequence: GTCA(underlined)(6N)T(underlined)GAY Escherichia coli ? - ? 382860