2.7.2.7 ATP + 2-ethylbutyrate - Listeria monocytogenes ADP + 2-ethylbutyryl phosphate - ? 459217 2.7.2.7 ATP + 2-ethylbutyrate 2.0% activity compared to valerate Desulfovibrio vulgaris ADP + 2-ethylbutyryl phosphate - ? 459217 2.7.2.7 ATP + 2-ethylbutyrate 2.0% activity compared to valerate Desulfovibrio vulgaris Hildenborough ADP + 2-ethylbutyryl phosphate - ? 459217 2.7.2.7 ATP + 2-methylbutyrate - Listeria monocytogenes ADP + 2-methylbutyryl phosphate - ? 459222 2.7.2.7 ATP + 2-methylbutyrate 22.8% activity compared to valerate Desulfovibrio vulgaris ADP + 2-methylbutyryl phosphate - ? 459222 2.7.2.7 ATP + 2-methylbutyrate 22.8% activity compared to valerate Desulfovibrio vulgaris Hildenborough ADP + 2-methylbutyryl phosphate - ? 459222 2.7.2.7 ATP + 2-methylpentanoate - Listeria monocytogenes ADP + 2-methylpentanoyl phosphate - ? 459223 2.7.2.7 ATP + 2-methylpentanoate 7.8% activity compared to valerate Desulfovibrio vulgaris ADP + 2-methylpentanoyl phosphate - ? 459223 2.7.2.7 ATP + 3-methylpentanoate - Listeria monocytogenes ADP + 3-methylpentanoyl phosphate - ? 459242 2.7.2.7 ATP + acetate - Listeria monocytogenes ADP + acetyl phosphate - ? 358044 2.7.2.7 ATP + acetate poor substrate Clostridium butyricum ADP + acetyl phosphate - ? 358044 2.7.2.7 ATP + acetate 6% of the activity with butanoate Clostridium acetobutylicum ADP + acetyl phosphate - ? 358044 2.7.2.7 ATP + acetate 3.8% activity compared to valerate Desulfovibrio vulgaris ADP + acetyl phosphate - ? 358044 2.7.2.7 ATP + acetate 3.8% activity compared to valerate Desulfovibrio vulgaris Hildenborough ADP + acetyl phosphate - ? 358044 2.7.2.7 ATP + butanoate - Clostridium butyricum ADP + butanoyl phosphate - ? 358065 2.7.2.7 ATP + butanoate - Clostridium butyricum ADP + butanoyl phosphate - r 358065 2.7.2.7 ATP + butanoate - Clostridium tetanomorphum ADP + butanoyl phosphate - ? 358065 2.7.2.7 ATP + butanoate - Clostridium acetobutylicum ADP + butanoyl phosphate - ? 358065 2.7.2.7 ATP + butanoate - Clostridium acetobutylicum ADP + butanoyl phosphate - r 358065 2.7.2.7 ATP + butanoate - Clostridium tyrobutyricum ADP + butanoyl phosphate - ? 358065 2.7.2.7 ATP + butanoate - Dasytricha ruminantium ADP + butanoyl phosphate - ? 358065 2.7.2.7 ATP + butanoate - Thermotoga maritima ADP + butanoyl phosphate - ? 358065 2.7.2.7 ATP + butanoate - Thermotoga maritima ADP + butanoyl phosphate - r 358065 2.7.2.7 ATP + butanoate third best substrate Listeria monocytogenes ADP + butanoyl phosphate - ? 358065 2.7.2.7 ATP + butanoate 52.3% activity compared to valerate Desulfovibrio vulgaris ADP + butanoyl phosphate - ? 358065 2.7.2.7 ATP + butanoate 52.3% activity compared to valerate Desulfovibrio vulgaris Hildenborough ADP + butanoyl phosphate - ? 358065 2.7.2.7 ATP + hexanoate - Listeria monocytogenes ADP + hexanoyl phosphate - ? 459380 2.7.2.7 ATP + hexanoate 16.3% activity compared to valerate Desulfovibrio vulgaris ADP + hexanoyl phosphate - ? 459380 2.7.2.7 ATP + isobutanoate - Clostridium butyricum ADP + isobutanoyl phosphate - ? 358067 2.7.2.7 ATP + isobutanoate - Clostridium tetanomorphum ADP + isobutanoyl phosphate - ? 358067 2.7.2.7 ATP + isobutanoate - Thermotoga maritima ADP + isobutanoyl phosphate - ? 358067 2.7.2.7 ATP + isobutanoate - Thermotoga maritima ADP + isobutanoyl phosphate - r 358067 2.7.2.7 ATP + isobutanoate 50% of the activity with butanoate Clostridium butyricum ADP + isobutanoyl phosphate - ? 358067 2.7.2.7 ATP + isobutanoate 54% of the activity with butanoate Clostridium acetobutylicum ADP + isobutanoyl phosphate - ? 358067 2.7.2.7 ATP + isobutyrate second best substrate Listeria monocytogenes ADP + isobutyroyl phosphate - ? 459392 2.7.2.7 ATP + isobutyrate 43.1% activity compared to valerate Desulfovibrio vulgaris ADP + isobutyroyl phosphate - ? 459392 2.7.2.7 ATP + isovalerate 32% of the activity with butanoate Clostridium acetobutylicum ADP + isopentanoyl phosphate - ? 358117 2.7.2.7 ATP + isovalerate - Listeria monocytogenes ADP + isovaleroyl phosphate - ? 459393 2.7.2.7 ATP + isovalerate 32.1% activity compared to valerate Desulfovibrio vulgaris ADP + isovaleroyl phosphate - ? 459393 2.7.2.7 ATP + pentanoate best substrate Listeria monocytogenes ADP + pentanoyl phosphate - ? 358064 2.7.2.7 ATP + propionate - Listeria monocytogenes ADP + propionyl phosphate - ? 358050 2.7.2.7 ATP + propionate 60.8% activity compared to valerate Desulfovibrio vulgaris ADP + propionyl phosphate - ? 358050 2.7.2.7 ATP + propionate 60.8% activity compared to valerate Desulfovibrio vulgaris Hildenborough ADP + propionyl phosphate - ? 358050 2.7.2.7 ATP + propionate - Clostridium butyricum ADP + propanoyl phosphate - ? 358066 2.7.2.7 ATP + propionate - Clostridium tetanomorphum ADP + propanoyl phosphate - ? 358066 2.7.2.7 ATP + propionate as active as butanoate Clostridium butyricum ADP + propanoyl phosphate - ? 358066 2.7.2.7 ATP + propionate 43% of the activity with butanoate Clostridium acetobutylicum ADP + propanoyl phosphate - ? 358066 2.7.2.7 ATP + valerate - Clostridium butyricum ADP + pentanoyl phosphate - ? 358115 2.7.2.7 ATP + valerate - Clostridium tetanomorphum ADP + pentanoyl phosphate - ? 358115 2.7.2.7 ATP + valerate 12% of the activity with butanoate Clostridium butyricum ADP + pentanoyl phosphate - ? 358115 2.7.2.7 ATP + valerate 89% of the activity with butanoate Clostridium acetobutylicum ADP + pentanoyl phosphate - ? 358115 2.7.2.7 ATP + valerate 100% activity Desulfovibrio vulgaris ADP + valeroyl phosphate - ? 459475 2.7.2.7 ATP + vinyl acetate 23% of the activity with butanoate Clostridium acetobutylicum ADP + vinylacetyl phosphate - ? 358118 2.7.2.7 additional information enzyme is involved in production of butyrate Dasytricha ruminantium ? - ? 89 2.7.2.7 additional information the enzyme is not regulated by the end-product, its specific activity is constant during the fermentation Clostridium acetobutylicum ? - ? 89 2.7.2.7 additional information semi-automated reverse engineering algorithm. The reconstructed metabolic network was used to create a genome-scale model that correctly characterized the butyrate kinase knock-out and the asolventogenic M5 pSOL1 megaplasmid degenerate strains. Systematic gene knock-out simulations performed to identify a set of genes encoding clostridial enzymes essential for growth in silico. Clostridium acetobutylicum ? - ? 89 2.7.2.7 additional information overexpressed PhaP1 from Ralstonia eutropha H16 affected poly(3-mercaptopropionate) [poly(3MP)] and poly(3-hydroxybutyrate) [poly(3HB)] accumulation in recombinant Escherichia coli, which expresses the non-natural BPEC pathway consisting of butyrate kinase and phosphotransbutyrylase from Clostridium acetobutylicum and PHA synthase from Thiococcus pfennigii Clostridium acetobutylicum ? - ? 89