2.6.1.98 additional information 2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid WbpE product identification by mass spectrometry, overview Pseudomonas aeruginosa ? - 89 2.6.1.98 additional information WbpB and WbpE are a dehydrogenase/aminotransferase pair that converts UDP-N-acetyl-D-glucosaminuronate, UDP-GlcNAcA, to UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronate, UDP-GlcNAc(3NH2)A, in a coupled reaction via a unique NAD+ recycling pathway, large-scale coupled reaction WpbB/WpbE, overview. WbpA, WbpB, WbpE, WbpD and WbpI can be combined in vitro to generate UDP-ManNAc(3NAc)A in a single reaction vessel. Addition of 0.2 mM NAD+, for WbpB, and 0.1 mM pyridoxal 5'-phosphate, for WbpE, to the reaction mixture aids in achieving complete turnover of substrate, which implies that the heterologously expressed proteins are not saturated with cofactor due to the limiting intracellular levels of both NAD+ and pyridoxal 5'-phosphate in Escherichia coli. WbpB/WbpE substrate specificity, overview Pseudomonas aeruginosa ? - 89 2.6.1.98 UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate - Pseudomonas aeruginosa UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate - 419614 2.6.1.98 UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate transaminase WbpE product is UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate Pseudomonas aeruginosa UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate - 419614 2.6.1.98 UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate WbpE/WlbC product is UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate Pseudomonas aeruginosa UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate - 419614 2.6.1.98 UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate WbpE/WlbC product is UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate Bordetella pertussis UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate - 419614 2.6.1.98 UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate transaminase WbpE product is UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate. Cofactor pyridoxal 5'-phosphate is converted to pyridoxamine phosphate during the reaction Pseudomonas aeruginosa UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate - 419614 2.6.1.98 UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate WbpE product is UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid Pseudomonas aeruginosa UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate - 419614 2.6.1.98 UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate - Pseudomonas aeruginosa ATCC 15692 UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate - 419614 2.6.1.98 UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate - Pseudomonas aeruginosa 1C UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate - 419614 2.6.1.98 UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate - Pseudomonas aeruginosa PRS 101 UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate - 419614 2.6.1.98 UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate - Pseudomonas aeruginosa DSM 22644 UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate - 419614 2.6.1.98 UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate - Pseudomonas aeruginosa CIP 104116 UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate - 419614 2.6.1.98 UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate - Pseudomonas aeruginosa LMG 12228 UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate - 419614 2.6.1.98 UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate - Pseudomonas aeruginosa JCM 14847 UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate - 419614