2.1.1.59 S-adenosyl-L-methionine + CNBr peptide - Neurospora crassa ? - ? 451882 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine - Saccharomyces cerevisiae S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine - ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine - Triticum aestivum S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine - ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine - Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine - ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine - Strigomonas oncopelti S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine - ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine avian cytochromes c are much better substrates than those from mammalian sources Triticum aestivum S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine - ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine specific for unmethylated cytochrome c Saccharomyces cerevisiae S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine - ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine specific for unmethylated cytochrome c Saccharomyces cerevisiae S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine a single lysine residue at position 72 of horse heart cytochrome c is methylated ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine specific for unmethylated cytochrome c Saccharomyces cerevisiae S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine 9:17:74 ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine specific for unmethylated cytochrome c Saccharomyces cerevisiae S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4 ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine recombinant substrate iso-1-apocytochrome c Saccharomyces cerevisiae S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4 ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine lysine-72 of horse cytochrome c is a poor acceptor Strigomonas oncopelti S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine uptake of methyl groups occurs only at lysine residue 8 and the terminal proline residue ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine Crithidia cytochrome c-557 is far the best substrate Strigomonas oncopelti S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine uptake of methyl groups occurs only at lysine residue 8 and the terminal proline residue ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine horse heart cytochrome c-72 Saccharomyces cerevisiae S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine - ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine horse heart cytochrome c-72 Triticum aestivum S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine - ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine horse heart cytochrome c-72 Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine - ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine horse heart cytochrome c-72 Saccharomyces cerevisiae S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine a single lysine residue at position 72 of horse heart cytochrome c is methylated ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine horse heart cytochrome c-72 Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine a single lysine residue at position 72 of horse heart cytochrome c is methylated ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine horse heart cytochrome c-72 Saccharomyces cerevisiae S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine 9:17:74 ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine horse heart cytochrome c-72 Saccharomyces cerevisiae S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4 ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine horse heart cytochrome c-72 Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4 ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine important features in determining methyl-accepting activity: 1. reactive site such as-X-Lys-Lys-Y-, 2. length of polypeptide chain, 3. tertiary structure Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine a single lysine residue at position 72 of horse heart cytochrome c is methylated ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine important features in determining methyl-accepting activity: 1. reactive site such as-X-Lys-Lys-Y-, 2. length of polypeptide chain, 3. tertiary structure Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4 ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine trimethylation of recombinant iso-1-cytochrome c of lysine at position 72 Saccharomyces cerevisiae S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine - ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine does not randomly methylate cytochrome c, but shows absolute specificity for some amino acid sequences Triticum aestivum S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine - ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine does not randomly methylate cytochrome c, but shows absolute specificity for some amino acid sequences Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine a single lysine residue at position 72 of horse heart cytochrome c is methylated ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine does not randomly methylate cytochrome c, but shows absolute specificity for some amino acid sequences Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4 ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine also CNBr peptides of horse heart cytochrome c can serve as substrates, overview Triticum aestivum S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine - ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine also CNBr peptides of horse heart cytochrome c can serve as substrates, overview Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine a single lysine residue at position 72 of horse heart cytochrome c is methylated ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine also CNBr peptides of horse heart cytochrome c can serve as substrates, overview Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4 ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine overview: substrate activity of various cytochromes c Triticum aestivum S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine - ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine overview: substrate activity of various cytochromes c Saccharomyces cerevisiae S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine a single lysine residue at position 72 of horse heart cytochrome c is methylated ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine overview: substrate activity of various cytochromes c Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine a single lysine residue at position 72 of horse heart cytochrome c is methylated ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine overview: substrate activity of various cytochromes c Saccharomyces cerevisiae S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine 9:17:74 ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine overview: substrate activity of various cytochromes c Saccharomyces cerevisiae S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4 ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine overview: substrate activity of various cytochromes c Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4 ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine in vivo methylation of cytochrome c in yeast occurs probably before cytochrome c is attached to the mitochondria Saccharomyces cerevisiae S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine - ? 319768 2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine regulation of enzyme and cytochrome c substrate is not in parallel manner Saccharomyces cerevisiae S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine - ? 319768 2.1.1.59 S-adenosyl-L-methionine + myoglobin L-lysine 10% activity compared to cytochrome c Neurospora crassa S-adenosyl-L-homocysteine + myoglobin N6-methyl-L-lysine - ? 319769 2.1.1.59 S-adenosyl-L-methionine + [apocytochrome c]-L-lysine - Triticum aestivum S-adenosyl-L-homocysteine + [apocytochrome c]-N6-methyl-L-lysine - r 451881 2.1.1.59 S-adenosyl-L-methionine + [cytochrome c]-L-lysine - Saccharomyces cerevisiae S-adenosyl-L-homocysteine + [cytochrome c]-N6-methyl-L-lysine - r 451880 2.1.1.59 S-adenosyl-L-methionine + [cytochrome c]-L-lysine - Neurospora crassa S-adenosyl-L-homocysteine + [cytochrome c]-N6-methyl-L-lysine - r 451880