2.1.1.267 2 S-adenosyl-L-methionine + myricetin the enzyme also catalyzes the methylation of luteolin, tricetin and caffeoyl-CoA. ROMT-15 exhibits similar kcat/Km values for the four substrates. ROMT-15 can not utilize naringenin, apigenin, or kaempferol. The 2,3-double bond and the O-dihydroxyl group are both required for catalytic activity Oryza sativa 2 S-adenosyl-L-homocysteine + syringetin syringetin is the 3',5'-dimethyl ether of myricetin ? 407786 2.1.1.267 2 S-adenosyl-L-methionine + myricetin the enzyme also catalyzes the methylation of luteolin, tricetin and caffeoyl-CoA. ROMT-17 prefers tricetin. ROMT-15 can not utilize naringenin, apigenin, or kaempferol. The 2,3-double bond and the O-dihydroxyl group are both required for catalytic activity Oryza sativa 2 S-adenosyl-L-homocysteine + syringetin syringetin is the 3',5'-dimethyl ether of myricetin ? 407786 2.1.1.267 4 S-adenosyl-L-methionine + quercetin whole-cell biocatalysis using CdFOMT5 expressed in Escherichia coli cells is performed using quercetin as a substrate, and 3,3',5,7-tetramethylated quercetin is obtained as the final product Citrus depressa 4 S-adenosyl-L-homocysteine + 3,3',5,7-tetramethoxyquercetin - ? 454630 2.1.1.267 eriodictyol + S-adenosyl-L-methionine 11% of the activity with quercetin Solanum lycopersicum homoeriodictyol + S-adenosyl-L-homocysteine - ? 433717 2.1.1.267 laricitrin + S-adenosyl-L-methionine 114% of the activity with quercetin Solanum lycopersicum syringetin + S-adenosyl-L-homocysteine - ? 433900 2.1.1.267 luteolin + S-adenosyl-L-methionine 42% of the activity with quercetin Solanum lycopersicum chrysoeriol + S-adenosyl-L-homocysteine - ? 433912 2.1.1.267 additional information substrate specificity, overview Nemophila menziesii ? - - 89 2.1.1.267 additional information CROMT2 is involved in biosynthesis of glycosides and anthocyanins Catharanthus roseus ? - ? 89 2.1.1.267 additional information isoform ROMT-15, no substrate: naringenin, apigenin, kaempferol Oryza sativa ? - ? 89 2.1.1.267 additional information isoform ROMT-17, no substrate: naringenin, apigenin, kaempferol Oryza sativa ? - ? 89 2.1.1.267 additional information OMT2 performs two sequential methylations at the 3'-and 5'-positions of the B-ring in myricetin (flavonol) and dihydromyricetin (dihydroflavonol) Catharanthus roseus ? - ? 89 2.1.1.267 additional information no activity with kaempferol, dihydrokaempferol, caffeate and caffeate esters Catharanthus roseus ? - ? 89 2.1.1.267 additional information enzyme is a regiospecific flavonoid 3'/5'-O-methyltransferase showing higher binding affinity and catalytic efficiency for quercetin and luteolin than for eriodictyol. No substrates: naringenin, apigenin, tricetin, kaempferol, daidzein, genistein Solanum lycopersicum ? - ? 89 2.1.1.267 additional information anthocyanin O-methyltransferase methylates anthocyanins of both groups, di- and trihydroxylated anthocyanins, quantitative trait locus analyses for the ratios of tri/di-hydroxylated and methylated/non-methylated anthocyanins using a population from an interspecific hybrid cross, relationship between the genotypes of the markers closest to the major quantitative trait loci and the expression levels of anthocyanin biosynthetic genes, overview Vitis vinifera ? - ? 89 2.1.1.267 additional information the enzyme AnthOMT shows a strong affinity for glycosylated anthocyanins, while other flavonoid glycosides and aglycones are much less preferred Solanum lycopersicum ? - ? 89 2.1.1.267 additional information the specificity of AnthOMT is mainly directed towards the 3' and 5' positions of the anthocyanin B-ring, to produce petunidin and malvidin glucosides, and is not active at the 4' position, substrate specificity of enzyme AnthOMT, overview. Poor activity with caffeic acid, no activity with pelargonidin-3-glucoside and delphinidin. AnthOMT is able to methylate the more complex substrates, e.g. an an extract of semi-polar compounds from Ros/Del tomato fruits preparation, resulting in a strong increase in malvidin 3-(4-coumaroyl)-rutinoside-5-glucoside Solanum lycopersicum ? - ? 89 2.1.1.267 additional information substrate specificity study using a number of potential substrates including anthocyanidins, anthocyanins, flavonols, flavones, flavan-3-ols, and phenolic acid as substrates based on optimized conditions, overview. Enzyme PsAOMT uses anthocyanins as methoxyl accepters, and acts to methylate the 3'-hydroxyl group of the B-ring with high affinity and efficiency. Pelargonidin 3-O-glucoside is the only tested anthocyanin compound that is not a substrate for PsAOMT. With delphinidin 3-O-glucoside, a sequential methylation occurs at the 3'- and 5'-hydroxyl group on the B-ring. In comparison with the substrates delphinidin 3-O-glucoside, quercetin 3-O-rutinoside, and quercetin, PsAOMT has a higher affinity for cyanidin 3-O-glucoside and cyanidin 3,5-O-diglucoside. Cyanidin-derived anthocyanins are high-affinity substrates for PsAOMT. No activity with the antocyanin pelargonidin 3-O-glucoside and the cyanidin delphinin. The enzyme also shows no activity with kaempferol 3-O-glucoside, kaempferol, apigenin, naringenin, epicatechin, and caffeic acid Paeonia suffruticosa ? - - 89 2.1.1.267 additional information substrate specificity study using a number of potential substrates including anthocyanidins, anthocyanins, flavonols, flavones, flavan-3-ols, and phenolic acid as substrates based on optimized conditions, overview. Enzyme PtAOMT uses anthocyanins as methoxyl accepters, and acts to methylate the 3'-hydroxyl group of the B-ring with high affinity and efficiency. Pelargonidin 3-O-glucoside is the only tested anthocyanin compound that is not a substrate for PtAOMT. With delphinidin 3-O-glucoside, a sequential methylation occurs at the 3'- and 5'-hydroxyl group on the B-ring. In comparison with the substrates delphinidin 3-O-glucoside, quercetin 3-O-rutinoside, and quercetin, PtAOMT has a higher affinity for cyanidin 3-O-glucoside and cyanidin 3,5-O-diglucoside. Cyanidin-derived anthocyanins are high-affinity substrates for PtAOMT. No activity with the antocyanin pelargonidin 3-O-glucoside and the cyanidin delphinin. The enzyme also shows no activity with kaempferol 3-O-glucoside, kaempferol, apigenin, naringenin, epicatechin, and caffeic acid Paeonia tenuifolia ? - - 89 2.1.1.267 additional information the flavonoid-O-methyltransferase from Citrus depressa has a broad substrate specificity and regioselectivity Citrus depressa ? - - 89 2.1.1.267 additional information the flavonoid-O-methyltransferase from Citrus depressa has a broad substrate specificity and regioselectivity. Isozyme CdFOMT5 exhibits O-methyltransferase activity for quercetin, naringenin, (-)-epicatechin, and equol using S-adenosyl-L-methionine (SAM) as a methyl donor. The recombinant CdFOMT5 CdFOMT5 can catalyze the O-methylation of at least three hydroxyl groups of quercetin, and di- or tri-O-methylated quercetin products are obtained by this enzymatic reaction. CdFMOT5 prefers flavonol (3-hydroxyflavone) to other flavonoid structures. Thus, substrate structure, especially the C-ring in flavonoids, may strongly affect the substrate preference, including regioselectivity, of CdFOMT5 Citrus depressa ? - - 89 2.1.1.267 quercetin + S-adenosyl-L-methionine - Solanum lycopersicum isorhamnetin + S-adenosyl-L-homocysteine - ? 434178 2.1.1.267 S-adenosyl-L-methionine + 2,3-dihydromyricetin - Catharanthus roseus S-adenosyl-L-homocysteine + 3'-O-methyl-2,3-dihydromyricetin - ? 424028 2.1.1.267 S-adenosyl-L-methionine + 3'-methoxydelphinidin 3-O-glucoside - Paeonia suffruticosa S-adenosyl-L-homocysteine + 3',5'-dimethoxydelphinidin 3-O-glucoside - ? 454485 2.1.1.267 S-adenosyl-L-methionine + 3'-methoxydelphinidin 3-O-glucoside - Paeonia tenuifolia S-adenosyl-L-homocysteine + 3',5'-dimethoxydelphinidin 3-O-glucoside - ? 454485 2.1.1.267 S-adenosyl-L-methionine + 3'-methoxydelphinidin 3-O-glucoside very low activity, with delphinidin 3-O-glucoside as substrate, NmAMT6 almost exclusively yields petunidin 3-O-glucoside rather than malvidin 3-O-glucoside. This specificity is consistent with the anthocyanin composition of Nemophila petals Nemophila menziesii S-adenosyl-L-homocysteine + 3',5'-dimethoxydelphinidin 3-O-glucoside - ? 454485 2.1.1.267 S-adenosyl-L-methionine + 3'-O-methyl-2,3-dihydromyricetin - Catharanthus roseus S-adenosyl-L-homocysteine + 3',5'-di-O-methyl-2,3-dihydromyricetin i.e. syringetin ? 424029 2.1.1.267 S-adenosyl-L-methionine + 3'-O-methyl-2,3-dihydroquercetin - Catharanthus roseus S-adenosyl-L-homocysteine + 3',4'-di-O-methyl-2,3-dihydroquercetin - ? 424030 2.1.1.267 S-adenosyl-L-methionine + 3'-O-methylmyricetin - Catharanthus roseus S-adenosyl-L-homocysteine + 3',5'-di-O-methylmyricetin - ? 424031 2.1.1.267 S-adenosyl-L-methionine + 3'-O-methylquercetin - Catharanthus roseus S-adenosyl-L-homocysteine + 3',4'-di-O-methylquercetin - ? 424032 2.1.1.267 S-adenosyl-L-methionine + 7,8-dihydroxyflavone - Oryza sativa S-adenosyl-L-homocysteine + ? - ? 406606 2.1.1.267 S-adenosyl-L-methionine + a 3'-hydroxyflavonoid - Paeonia suffruticosa S-adenosyl-L-homocysteine + a 3'-methoxyflavonoid - ? 454495 2.1.1.267 S-adenosyl-L-methionine + a 3'-hydroxyflavonoid - Solanum lycopersicum S-adenosyl-L-homocysteine + a 3'-methoxyflavonoid - ? 454495 2.1.1.267 S-adenosyl-L-methionine + a 3'-hydroxyflavonoid - Paeonia tenuifolia S-adenosyl-L-homocysteine + a 3'-methoxyflavonoid - ? 454495 2.1.1.267 S-adenosyl-L-methionine + caffeoyl-coenzyme A isoform ROMT-15, 100% of the activity with myricetin Oryza sativa S-adenosyl-L-homocysteine + ? - ? 406611 2.1.1.267 S-adenosyl-L-methionine + caffeoyl-coenzyme A isoform ROMT-17, 100% of the activity with myricetin Oryza sativa S-adenosyl-L-homocysteine + ? - ? 406611 2.1.1.267 S-adenosyl-L-methionine + cyanidin very low activity Paeonia suffruticosa S-adenosyl-L-homocysteine + 3'-methoxycyanidin - ? 456448 2.1.1.267 S-adenosyl-L-methionine + cyanidin 3,5-O-diglucoside - Paeonia tenuifolia S-adenosyl-L-homocysteine + 3'-methoxycyanidin 3,5-O-diglucoside - ? 456443 2.1.1.267 S-adenosyl-L-methionine + cyanidin 3,5-O-diglucoside substrate binding structure, modelling, overview Paeonia suffruticosa S-adenosyl-L-homocysteine + 3'-methoxycyanidin 3,5-O-diglucoside - ? 456443 2.1.1.267 S-adenosyl-L-methionine + cyanidin 3,5-O-diglucoside - Torenia hybrid cultivar S-adenosyl-L-homocysteine + peonidin 3,5-O-diglucoside - ? 456444 2.1.1.267 S-adenosyl-L-methionine + cyanidin 3-O-beta-D-glucoside - Vitis vinifera S-adenosyl-L-homocysteine + peonidin 3-O-beta-D-glucoside + H+ - ? 422053 2.1.1.267 S-adenosyl-L-methionine + cyanidin 3-O-beta-D-glucoside - Solanum lycopersicum S-adenosyl-L-homocysteine + peonidin 3-O-beta-D-glucoside - ? 435794 2.1.1.267 S-adenosyl-L-methionine + cyanidin 3-O-glucoside - Paeonia suffruticosa S-adenosyl-L-homocysteine + 3'-methoxycyanidin 3-O-glucoside - ? 456446 2.1.1.267 S-adenosyl-L-methionine + cyanidin 3-O-glucoside - Paeonia tenuifolia S-adenosyl-L-homocysteine + 3'-methoxycyanidin 3-O-glucoside - ? 456446 2.1.1.267 S-adenosyl-L-methionine + cyanidin 3-O-glucoside - Torenia hybrid cultivar S-adenosyl-L-homocysteine + peonidin 3-O-glucoside - ? 456447 2.1.1.267 S-adenosyl-L-methionine + cyanidin 3-O-glucoside-5-O-coumaroylglucoside - Torenia hybrid cultivar S-adenosyl-L-homocysteine + peonidin 3-O-glucoside-5-O-coumaroylglucoside - ? 456445 2.1.1.267 S-adenosyl-L-methionine + delphinidin 3,5-O-diglucoside - Torenia hybrid cultivar S-adenosyl-L-homocysteine + petunidin 3,5-O-diglucoside - ? 456463 2.1.1.267 S-adenosyl-L-methionine + delphinidin 3-O-beta-D-glucoside high activity Solanum lycopersicum S-adenosyl-L-homocysteine + petunidin 3-O-beta-D-glucoside - ? 435796 2.1.1.267 S-adenosyl-L-methionine + delphinidin 3-O-glucoside - Vitis vinifera S-adenosyl-L-homocysteine + petunidin 3-O-beta-D-glucoside - ? 422054 2.1.1.267 S-adenosyl-L-methionine + delphinidin 3-O-glucoside - Paeonia suffruticosa S-adenosyl-L-homocysteine + 3'-methoxydelphinidin 3-O-glucoside - ? 454605 2.1.1.267 S-adenosyl-L-methionine + delphinidin 3-O-glucoside - Nemophila menziesii S-adenosyl-L-homocysteine + 3'-methoxydelphinidin 3-O-glucoside - ? 454605 2.1.1.267 S-adenosyl-L-methionine + delphinidin 3-O-glucoside - Paeonia tenuifolia S-adenosyl-L-homocysteine + 3'-methoxydelphinidin 3-O-glucoside - ? 454605 2.1.1.267 S-adenosyl-L-methionine + delphinidin 3-O-glucoside with delphinidin 3-O-glucoside as substrate, NmAMT6 almost exclusively yields petunidin 3-O-glucoside rather than malvidin 3-O-glucoside. This specificity is consistent with the anthocyanin composition of Nemophila petals Nemophila menziesii S-adenosyl-L-homocysteine + 3'-methoxydelphinidin 3-O-glucoside - ? 454605 2.1.1.267 S-adenosyl-L-methionine + delphinidin 3-O-glucoside - Torenia hybrid cultivar S-adenosyl-L-homocysteine + petunidin 3-O-glucoside - ? 456465 2.1.1.267 S-adenosyl-L-methionine + delphinidin 3-O-glucoside-5-O-coumaroylglucoside - Torenia hybrid cultivar S-adenosyl-L-homocysteine + petunidin 3-O-glucoside-5-O-coumaroylglucoside - ? 456464 2.1.1.267 S-adenosyl-L-methionine + dihydromyricetin two sequential methylations at the 3'- and 5'-positions of the B-ring in dihydromyricetin Catharanthus roseus ? - ? 354291 2.1.1.267 S-adenosyl-L-methionine + dihydromyricetin 54% of the activity with myricetin Catharanthus roseus S-adenosyl-L-homocysteine + ? - ? 377688 2.1.1.267 S-adenosyl-L-methionine + dihydroquercetin 22% of the activity with myricetin Catharanthus roseus S-adenosyl-L-homocysteine + ? - ? 377689 2.1.1.267 S-adenosyl-L-methionine + dihydroquercetin - Catharanthus roseus S-adenosyl-L-homocysteine + 3'-O-methyl-2,3-dihydroquercetin - ? 424048 2.1.1.267 S-adenosyl-L-methionine + fisetin analysis of binding constant and docking energy Halalkalibacterium halodurans S-adenosyl-L-homocysteine + ? - ? 398281 2.1.1.267 S-adenosyl-L-methionine + luteolin analysis of binding constant and docking energy Halalkalibacterium halodurans S-adenosyl-L-homocysteine + ? - ? 406617 2.1.1.267 S-adenosyl-L-methionine + luteolin isoform ROMT-15, 92% of the activity with myricetin Oryza sativa S-adenosyl-L-homocysteine + ? isoform ROMT-15, methylation at 3'-hydroxyl group ? 406617 2.1.1.267 S-adenosyl-L-methionine + luteolin isoform ROMT-17, 92% of the activity with myricetin Oryza sativa S-adenosyl-L-homocysteine + ? isoform ROMT-17, methylation at 3'-hydroxyl group ? 406617 2.1.1.267 S-adenosyl-L-methionine + luteolin very low activity Paeonia suffruticosa S-adenosyl-L-homocysteine + 3'-methoxyluteolin - ? 456562 2.1.1.267 S-adenosyl-L-methionine + luteolin low activity Paeonia tenuifolia S-adenosyl-L-homocysteine + 3'-methoxyluteolin - ? 456562 2.1.1.267 S-adenosyl-L-methionine + luteolin 7-O-glucoside low activity Paeonia suffruticosa S-adenosyl-L-homocysteine + 3'-methoxyluteolin 7-O-glucoside - ? 456560 2.1.1.267 S-adenosyl-L-methionine + luteolin 7-O-glucoside low activity Paeonia tenuifolia S-adenosyl-L-homocysteine + 3'-methoxyluteolin 7-O-glucoside - ? 456560 2.1.1.267 S-adenosyl-L-methionine + myricetin analysis of binding constant and docking energy Halalkalibacterium halodurans S-adenosyl-L-homocysteine + ? - ? 319873 2.1.1.267 S-adenosyl-L-methionine + myricetin two sequential methylations at the 3'- and 5'-positions of the B-ring in myricetin, activity is strictly confined to flavonols and dihydroflavonols, it requires at least 2 B-ring hydroxyl groups Catharanthus roseus ? - ? 354290 2.1.1.267 S-adenosyl-L-methionine + myricetin involvement in biosynthesis of flavonol glycosides and anthocyanins Catharanthus roseus ? - ? 354290 2.1.1.267 S-adenosyl-L-methionine + myricetin - Catharanthus roseus S-adenosyl-L-homocysteine + syringetin - ? 377715 2.1.1.267 S-adenosyl-L-methionine + myricetin - Oryza sativa S-adenosyl-L-homocysteine + syringetin isoform ROMT-15, methylation at 3'-hydroxyl group and at the 5' hydroxyl group ? 377715 2.1.1.267 S-adenosyl-L-methionine + myricetin - Oryza sativa S-adenosyl-L-homocysteine + syringetin isoform ROMT-17, methylation at 3'-hydroxyl group and at the 5' hydroxyl group ? 377715 2.1.1.267 S-adenosyl-L-methionine + myricetin best substrate Catharanthus roseus S-adenosyl-L-homocysteine + 3'-O-methylmyricetin - ? 424069 2.1.1.267 S-adenosyl-L-methionine + myricitin - Solanum lycopersicum S-adenosyl-L-homocysteine + laricitrin two product isomers ? 435808 2.1.1.267 S-adenosyl-L-methionine + petunidin 3,5-O-diglucoside - Torenia hybrid cultivar S-adenosyl-L-homocysteine + malvidin 3,5-O-diglucoside - ? 456584 2.1.1.267 S-adenosyl-L-methionine + petunidin 3-O-beta-D-glucoside - Vitis vinifera S-adenosyl-L-homocysteine + malvidin 3-O-beta-D-glucoside - ? 422066 2.1.1.267 S-adenosyl-L-methionine + petunidin 3-O-glucoside - Torenia hybrid cultivar S-adenosyl-L-homocysteine + malvidin 3-O-glucoside - ? 456586 2.1.1.267 S-adenosyl-L-methionine + petunidin 3-O-glucoside-5-O-coumaroylglucoside - Torenia hybrid cultivar S-adenosyl-L-homocysteine + malvidin 3-O-glucoside-5-O-coumaroylglucoside - ? 456585 2.1.1.267 S-adenosyl-L-methionine + quercetin - Oryza sativa S-adenosyl-L-homocysteine + ? isoform ROMT-15, methylation at 3'-hydroxyl group ? 385690 2.1.1.267 S-adenosyl-L-methionine + quercetin - Oryza sativa S-adenosyl-L-homocysteine + ? isoform ROMT-17, methylation at 3'-hydroxyl group ? 385690 2.1.1.267 S-adenosyl-L-methionine + quercetin analysis of binding constant and docking energy Halalkalibacterium halodurans S-adenosyl-L-homocysteine + ? - ? 385690 2.1.1.267 S-adenosyl-L-methionine + quercetin low activity Catharanthus roseus S-adenosyl-L-homocysteine + 3'-O-methylquercetin - ? 424070 2.1.1.267 S-adenosyl-L-methionine + quercetin - Solanum lycopersicum S-adenosyl-L-homocysteine + isorhamnetin - ? 434259 2.1.1.267 S-adenosyl-L-methionine + quercetin - Paeonia suffruticosa S-adenosyl-L-homocysteine + 3'-methoxyquercetin - ? 456599 2.1.1.267 S-adenosyl-L-methionine + quercetin - Citrus depressa S-adenosyl-L-homocysteine + 3'-methoxyquercetin - ? 456599 2.1.1.267 S-adenosyl-L-methionine + quercetin - Paeonia tenuifolia S-adenosyl-L-homocysteine + 3'-methoxyquercetin - ? 456599 2.1.1.267 S-adenosyl-L-methionine + quercetin - Citrus depressa S-adenosyl-L-homocysteine + 3-methoxyquercetin - ? 456600 2.1.1.267 S-adenosyl-L-methionine + quercetin - Citrus depressa S-adenosyl-L-homocysteine + 5-methoxyquercetin - ? 456601 2.1.1.267 S-adenosyl-L-methionine + quercetin - Citrus depressa S-adenosyl-L-homocysteine + 7-methoxyquercetin - ? 456603 2.1.1.267 S-adenosyl-L-methionine + quercetin 3-O-beta-D-glucoside - Solanum lycopersicum S-adenosyl-L-homocysteine + isorhamnetin 3-O-beta-D-glucoside - ? 435815 2.1.1.267 S-adenosyl-L-methionine + quercetin 3-O-beta-D-rutinoside - Solanum lycopersicum S-adenosyl-L-homocysteine + isorhamnetin 3-O-beta-D-rutinoside - ? 435816 2.1.1.267 S-adenosyl-L-methionine + quercetin 3-O-rutinoside - Paeonia suffruticosa S-adenosyl-L-homocysteine + 3'-methoxyquercetin 3-O-rutinoside - ? 456597 2.1.1.267 S-adenosyl-L-methionine + quercetin 3-O-rutinoside - Paeonia tenuifolia S-adenosyl-L-homocysteine + 3'-methoxyquercetin 3-O-rutinoside - ? 456597 2.1.1.267 S-adenosyl-L-methionine + tricetin isoform ROMT-15, methylation at 3'-hydroxyl group and at the 5' hydroxyl group. 87% of the activity with myricetin Oryza sativa S-adenosyl-L-homocysteine + ? - ? 406622 2.1.1.267 S-adenosyl-L-methionine + tricetin isoform ROMT-17, methylation at 3'-hydroxyl group and at the 5' hydroxyl group. 87% of the activity with myricetin Oryza sativa S-adenosyl-L-homocysteine + ? - ? 406622