2.1.1.244 2 S-adenosyl-L-methionine + CPKRIA mono- and dimethylation by NTMT1 Homo sapiens 2 S-adenosyl-L-homocysteine + ? - ? 454840 2.1.1.244 2 S-adenosyl-L-methionine + FPKRIA dimethylation and low level trimethylation by NTMT1 Homo sapiens 2 S-adenosyl-L-homocysteine + ? - ? 454845 2.1.1.244 2 S-adenosyl-L-methionine + HPKRIA dimethylation by NTMT1 Homo sapiens 2 S-adenosyl-L-homocysteine + ? - ? 454848 2.1.1.244 2 S-adenosyl-L-methionine + IPKRIA mono- and dimethylation by NTMT1 Homo sapiens 2 S-adenosyl-L-homocysteine + ? - ? 454849 2.1.1.244 2 S-adenosyl-L-methionine + KPKRIA dimethylation and low level trimethylation by NTMT1 Homo sapiens 2 S-adenosyl-L-homocysteine + ? - ? 454850 2.1.1.244 2 S-adenosyl-L-methionine + LPKRIA dimethylation and low level trimethylation by NTMT1 Homo sapiens 2 S-adenosyl-L-homocysteine + ? - ? 454851 2.1.1.244 2 S-adenosyl-L-methionine + MPKRIA dimethylation and low level trimethylation by NTMT1 Homo sapiens 2 S-adenosyl-L-homocysteine + ? - ? 454853 2.1.1.244 2 S-adenosyl-L-methionine + N-terminal-histone 2B dimethylation of fruit fly histone 2B by NTMT1 over an N-terminal sequence of 1PPKTSGKAA9 Homo sapiens 2 S-adenosyl-L-homocysteine + ? - ? 454854 2.1.1.244 2 S-adenosyl-L-methionine + NPKRIA dimethylation by NTMT1 Homo sapiens 2 S-adenosyl-L-homocysteine + ? - ? 454855 2.1.1.244 2 S-adenosyl-L-methionine + PPKRIA dimethylation by NTMT1 Homo sapiens 2 S-adenosyl-L-homocysteine + ? - ? 454857 2.1.1.244 2 S-adenosyl-L-methionine + QPKRIA dimethylation by NTMT1 Homo sapiens 2 S-adenosyl-L-homocysteine + ? - ? 454858 2.1.1.244 2 S-adenosyl-L-methionine + RPKRIA dimethylation and low level trimethylation by NTMT1 Homo sapiens 2 S-adenosyl-L-homocysteine + ? - ? 454859 2.1.1.244 2 S-adenosyl-L-methionine + TPKRIA mono- and dimethylation by NTMT1 Homo sapiens 2 S-adenosyl-L-homocysteine + ? - ? 454860 2.1.1.244 2 S-adenosyl-L-methionine + VPKRIA mono- and dimethylation by NTMT1 Homo sapiens 2 S-adenosyl-L-homocysteine + ? - ? 454862 2.1.1.244 2 S-adenosyl-L-methionine + WPKRIA mono- and dimethylation by NTMT1 Homo sapiens 2 S-adenosyl-L-homocysteine + ? - ? 454863 2.1.1.244 2 S-adenosyl-L-methionine + YPKRIA dimethylation by NTMT1 Homo sapiens 2 S-adenosyl-L-homocysteine + ? - ? 454864 2.1.1.244 3 (E)-hex-2-en-5-ynyl-S-adenosyl-L-methionine + N-terminal-OLA1 - Homo sapiens ? - ? 454950 2.1.1.244 3 S-adenosyl-L-methionine + APKRIA trimethylation by NTMT1 Homo sapiens 3 S-adenosyl-L-homocysteine + ? - ? 454960 2.1.1.244 3 S-adenosyl-L-methionine + eukaryotic elongation factor 1A enzyme N6AMT2 trimethylates eEF1A at the N-terminal site and at the adjacent lysine 79, protein substrate from Homo sapiens or Saccharomyces cerevisiae Saccharomyces cerevisiae 3 S-adenosyl-L-homocysteine + ? - ? 454182 2.1.1.244 3 S-adenosyl-L-methionine + eukaryotic elongation factor 1A enzyme N6AMT2 trimethylates eEF1A at the N-terminal site and at the adjacent lysine 79, protein substrate from Homo sapiens or Saccharomyces cerevisiae Homo sapiens 3 S-adenosyl-L-homocysteine + ? - ? 454182 2.1.1.244 3 S-adenosyl-L-methionine + eukaryotic elongation factor 1A enzyme N6AMT2 trimethylates eEF1A at the N-terminal site and at the adjacent lysine 79, protein substrate from Homo sapiens or Saccharomyces cerevisiae. Yeast eEF1A is trimethylated at its N-terminus and dimethylated at lysine 3. Human eEF1A is trimethylated at its N-terminus Saccharomyces cerevisiae 3 S-adenosyl-L-homocysteine + ? - ? 454182 2.1.1.244 3 S-adenosyl-L-methionine + eukaryotic elongation factor 1A enzyme N6AMT2 trimethylates eEF1A at the N-terminal site and at the adjacent lysine 79, protein substrate from Homo sapiens or Saccharomyces cerevisiae. Yeast eEF1A is trimethylated at its N-terminus and dimethylated at lysine 3. Human eEF1A is trimethylated at its N-terminus Homo sapiens 3 S-adenosyl-L-homocysteine + ? - ? 454182 2.1.1.244 3 S-adenosyl-L-methionine + eukaryotic elongation factor 1A enzyme YLR285W trimethylates eEF1A at the N-terminal site and at the adjacent lysine 79, protein substrate from Homo sapiens or Saccharomyces cerevisiae. Yeast eEF1A is trimethylated at its N-terminus and dimethylated at lysine 3. Methylation by Efm7 is affected by the conformation of eEF1A. Efm7 is unable to methylate a synthetic peptide corresponding to the N-terminal 10 amino acids of eEF1A (GKEKSHINVV), but methylates full-length eEF1A in vitro. Human eEF1A is trimethylated at its N-terminus Saccharomyces cerevisiae 3 S-adenosyl-L-homocysteine + ? - ? 454182 2.1.1.244 3 S-adenosyl-L-methionine + eukaryotic elongation factor 1A enzyme N6AMT2 trimethylates eEF1A at the N-terminal site and at the adjacent lysine 79, protein substrate from Homo sapiens or Saccharomyces cerevisiae Saccharomyces cerevisiae ATCC 204508 3 S-adenosyl-L-homocysteine + ? - ? 454182 2.1.1.244 3 S-adenosyl-L-methionine + eukaryotic elongation factor 1A enzyme N6AMT2 trimethylates eEF1A at the N-terminal site and at the adjacent lysine 79, protein substrate from Homo sapiens or Saccharomyces cerevisiae. Yeast eEF1A is trimethylated at its N-terminus and dimethylated at lysine 3. Human eEF1A is trimethylated at its N-terminus Saccharomyces cerevisiae ATCC 204508 3 S-adenosyl-L-homocysteine + ? - ? 454182 2.1.1.244 3 S-adenosyl-L-methionine + eukaryotic elongation factor 1A enzyme YLR285W trimethylates eEF1A at the N-terminal site and at the adjacent lysine 79, protein substrate from Homo sapiens or Saccharomyces cerevisiae. Yeast eEF1A is trimethylated at its N-terminus and dimethylated at lysine 3. Methylation by Efm7 is affected by the conformation of eEF1A. Efm7 is unable to methylate a synthetic peptide corresponding to the N-terminal 10 amino acids of eEF1A (GKEKSHINVV), but methylates full-length eEF1A in vitro. Human eEF1A is trimethylated at its N-terminus Saccharomyces cerevisiae ATCC 204508 3 S-adenosyl-L-homocysteine + ? - ? 454182 2.1.1.244 3 S-adenosyl-L-methionine + GPKRIA trimethylation by NTMT1 Homo sapiens 3 S-adenosyl-L-homocysteine + ? - ? 454183 2.1.1.244 3 S-adenosyl-L-methionine + N-terminal-CENP-A human CENP-A histone, molecular details for CENP-A recognition by NRMT1. State-specific trimethylation of CENP-A by NRMT1 Homo sapiens 3 S-adenosyl-L-homocysteine + ? - ? 454961 2.1.1.244 3 S-adenosyl-L-methionine + N-terminal-dimethyl-SPKRIAKRRS-[RCC1] - Homo sapiens 3 S-adenosyl-L-homocysteine + N-terminal-trimethyl-SPKRIAKRRS-[RCC1] - ? 435074 2.1.1.244 3 S-adenosyl-L-methionine + N-terminal-LPKRIA-[RCC1] - Homo sapiens 3 S-adenosyl-L-homocysteine + N-terminal-trimethyl-LPKRIA-[RCC1] - ? 435075 2.1.1.244 3 S-adenosyl-L-methionine + N-terminal-methyl-SPKRIAKRRS-[RCC1] - Homo sapiens 3 S-adenosyl-L-homocysteine + N-terminal-trimethyl-SPKRIAKRRS-[RCC1] - ? 435076 2.1.1.244 3 S-adenosyl-L-methionine + N-terminal-OLA1 i.e. Obg-like ATPase 1 (OLA1) protein, target validation using normal and NTMT1 knockout HEK-293FT cells demonstrates that OLA1, a protein involved in many critical cellular functions, is methylated in vivo by NTMT1 Homo sapiens 3 S-adenosyl-L-homocysteine + ? - ? 454185 2.1.1.244 3 S-adenosyl-L-methionine + N-terminal-OLA1 i.e. Obg-like ATPase 1 (OLA1) protein Homo sapiens 3 S-adenosyl-L-homocysteine + ? - ? 454185 2.1.1.244 3 S-adenosyl-L-methionine + N-terminal-peptide-[BAP1 protein] i.e. BRCA1 associated protein 1, a DNA repair protein Homo sapiens 3 S-adenosyl-L-homocysteine + N-terminal-trimethyl-peptide-[BAP1 protein] - ? 435077 2.1.1.244 3 S-adenosyl-L-methionine + N-terminal-peptide-[DDB2 protein] DDB2 is a DNA repair protein Homo sapiens 3 S-adenosyl-L-homocysteine + N-terminal-trimethyl-peptide-[DDB2 protein] - ? 435078 2.1.1.244 3 S-adenosyl-L-methionine + N-terminal-peptide-[PARP3 protein] i.e. poly-ADP-ribosylase 3, a DNA repair protein Homo sapiens 3 S-adenosyl-L-homocysteine + N-terminal-trimethyl-peptide-[PARP3 protein] - ? 435079 2.1.1.244 3 S-adenosyl-L-methionine + N-terminal-PPKRIA-[RCC1] - Homo sapiens 3 S-adenosyl-L-homocysteine + N-terminal-trimethyl-PPKRIA-[RCC1] - ? 435080 2.1.1.244 3 S-adenosyl-L-methionine + N-terminal-PPKRIA-[RCC1] best substrate Homo sapiens 3 S-adenosyl-L-homocysteine + N-terminal-trimethyl-PPKRIA-[RCC1] - ? 435080 2.1.1.244 3 S-adenosyl-L-methionine + N-terminal-RPKRIA-[RCC1] - Homo sapiens 3 S-adenosyl-L-homocysteine + N-terminal-trimethyl-RPKRIA-[RCC1] - ? 435081 2.1.1.244 3 S-adenosyl-L-methionine + N-terminal-SPKRIA-[RCC1] - Homo sapiens 3 S-adenosyl-L-homocysteine + N-terminal-trimethyl-SPKRIA-[RCC1] - ? 435082 2.1.1.244 3 S-adenosyl-L-methionine + N-terminal-SPKRIA-[RCC1] high activity Homo sapiens 3 S-adenosyl-L-homocysteine + N-terminal-trimethyl-SPKRIA-[RCC1] - ? 435082 2.1.1.244 3 S-adenosyl-L-methionine + N-terminal-SPKRIA-[RCC1] i.e. regulator of chromosome condensation 1 Homo sapiens 3 S-adenosyl-L-homocysteine + N-terminal-trimethyl-SPKRIA-[RCC1] - ? 435082 2.1.1.244 3 S-adenosyl-L-methionine + N-terminal-SPKRIAKRR-[RCC1] - Homo sapiens 3 S-adenosyl-L-homocysteine + N-terminal-trimethyl-SPKRIAKRR-[RCC1] - ? 435083 2.1.1.244 3 S-adenosyl-L-methionine + N-terminal-SPKRIAKRRS-[RCC1] - Homo sapiens 3 S-adenosyl-L-homocysteine + N-terminal-trimethyl-SPKRIAKRRS-[RCC1] - ? 435084 2.1.1.244 3 S-adenosyl-L-methionine + N-terminal-SPKRIAKRRSPP-[RCC1] - Homo sapiens 3 S-adenosyl-L-homocysteine + N-terminal-trimethyl-SPKRIAKRRSPP-[RCC1] - ? 435085 2.1.1.244 3 S-adenosyl-L-methionine + N-terminal-WPKRIA-[RCC1] - Homo sapiens 3 S-adenosyl-L-homocysteine + N-terminal-trimethyl-WPKRIA-[RCC1] - ? 435086 2.1.1.244 3 S-adenosyl-L-methionine + N-terminal-YPKRIA-[RCC1] - Homo sapiens 3 S-adenosyl-L-homocysteine + N-terminal-trimethyl-YPKRIA-[RCC1] - ? 435087 2.1.1.244 3 S-adenosyl-L-methionine + N-terminal-[eEF1A] - Saccharomyces cerevisiae 3 S-adenosyl-L-homocysteine + ? - ? 454186 2.1.1.244 3 S-adenosyl-L-methionine + N-terminal-[eEF1A] - Homo sapiens 3 S-adenosyl-L-homocysteine + ? - ? 454186 2.1.1.244 3 S-adenosyl-L-methionine + N-terminal-[eEF1A] specifically, the C-terminal domain is able to methylate peptides derived from the first 15 amino acids of eEF1A, whereas the N-terminal domain is sufficient for methylation of Lys55. High specificity of METTL13 for eEF1A Homo sapiens 3 S-adenosyl-L-homocysteine + ? - ? 454186 2.1.1.244 3 S-adenosyl-L-methionine + N-terminal-[eEF1A] - Saccharomyces cerevisiae ATCC 204508 3 S-adenosyl-L-homocysteine + ? - ? 454186 2.1.1.244 3 S-adenosyl-L-methionine + N-terminal-[RCC1] - Homo sapiens 3 S-adenosyl-L-homocysteine + ? - ? 454187 2.1.1.244 3 S-adenosyl-L-methionine + N-terminal-[RCC1] RCC1p and methionine-removed RCC1 Homo sapiens 3 S-adenosyl-L-homocysteine + ? - ? 454187 2.1.1.244 3 S-adenosyl-L-methionine + SPKRIA trimethylation by NTMT1 Homo sapiens 3 S-adenosyl-L-homocysteine + ? - ? 454962 2.1.1.244 L-lysyl-[protein] + 3 S-adenosyl-L-methionine - Escherichia coli 3 H+ + N6,N6,N6-trimethyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine - ? 454405 2.1.1.244 L-lysyl-[protein] + 3 S-adenosyl-L-methionine - Thermus thermophilus 3 H+ + N6,N6,N6-trimethyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine - ? 454405 2.1.1.244 L-lysyl-[protein] + 3 S-adenosyl-L-methionine - Thermus thermophilus DSM 579 3 H+ + N6,N6,N6-trimethyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine - ? 454405 2.1.1.244 L-lysyl-[protein] + 3 S-adenosyl-L-methionine - Thermus thermophilus ATCC 27634 3 H+ + N6,N6,N6-trimethyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine - ? 454405 2.1.1.244 additional information the N-terminal protein methyltransferase catalyzes the modification of two ribosomal protein substrates, Rpl12ab and Rps25a/Rps25b. The yeast RPS25A and RPS25B genes and differ only at a single amino acid residue Saccharomyces cerevisiae ? - ? 89 2.1.1.244 additional information enzyme substrates have a unique N-terminal motif, Met-(Ala/Pro/Ser)-Pro-Lys. The initiating Met is cleaved, and the exposed alpha-amino group is mono-, di-, or trimethylated Homo sapiens ? - ? 89 2.1.1.244 additional information the enzyme alpha-N-methylates the small subunit of ribulose-1,5-bisphohate carboxylase/oxygenase Spinacia oleracea ? - ? 89 2.1.1.244 additional information the enzyme shows a ternary complex mechanism of catalysis, involving formation of a SAM-enzyme-acceptor complex and direct transfer of the methyl group from SAM to the acceptor protein Homo sapiens ? - ? 89 2.1.1.244 additional information the methyltransferases specifically recognizes the N-terminal X-Pro-Lys sequence motif. Localization of methylation sites by top down mass spectrometry using collisionally activated dissociation or electron capture dissociation. The enzyme can also recognize species with N-terminal alanine and serine residues in addition to those with proline residues, but the proline residue in position 1 is a preferred substrate Homo sapiens ? - ? 89 2.1.1.244 additional information the methyltransferases specifically recognizes the N-terminal X-Pro-Lys sequence motif. Localization of methylation sites by top down mass spectrometry using collisionally activated dissociation or electron capture dissociation. The yeast enzyme can also recognize species with N-terminal alanine and serine residues in addition to those with proline residues, although to a lesser extent, the proline residue in position 1 is a preferred substrate Saccharomyces cerevisiae ? - ? 89 2.1.1.244 additional information the protein N-terminal methyltransferase 1 (NTMT1) methylates the alpha-N-terminal amines of proteins Homo sapiens ? - ? 89 2.1.1.244 additional information enzyme NTMT1 catalyzes the transfer of the methyl group from the S-adenosyl-L-methionine to the protein alpha-amine, resulting in formation of S-adenosyl-L-homocysteine and alpha-N-methylated proteins. Inhibition pattern and methylation progress analyses are performed to determine the kinetic mechanism and processivity of NTMT1, the enzyme NTMT1 utilizes a random sequential bi bi mechanism and proceeds in a distributive manner. Residues of RCC1, i.e. regulator of chromosome condensation 1, are recognized by the enzyme. Methylation status of products is analyzed by MALDI-mass spectrometry Homo sapiens ? - ? 89 2.1.1.244 additional information alpha-N-terminal methylation of histone H2B protein in Drosophila melanogaster Drosophila melanogaster ? - - 89 2.1.1.244 additional information human MTase-like protein 13 (METTL13) is a dual MTase for both N-terminal Gly1 and Lys55 of human eEF1A. To date, eEF1A is the only validated biological substrate for METTL13 Homo sapiens ? - - 89 2.1.1.244 additional information PrmA preferentially methylates free ribosomal protein L11 over an assembled 50S ribosomal subunit Escherichia coli ? - - 89 2.1.1.244 additional information protein N-terminal methyltransferase 1 (NTMT1/NRMT1) catalyzes the transfer of a methyl group from S-adenosyl-L-methionine (SAM) to protein alpha-N-terminal amines. It recognizes a specific motif X-P-K/R (X represents any amino acid other than D/E) Homo sapiens ? - - 89 2.1.1.244 additional information substrate of NTMT1 are regulator of chromosome condensation 1 (RCC1), tumor suppressor retinoblastoma1 (RB1), oncoprotein SET (also known as I2PP2A, TAF1a), damaged DNA-binding protein2 (DDB2), poly(ADP-ribose) polymerase3 (PARP3), and centromere proteins A and B Homo sapiens ? - - 89 2.1.1.244 additional information the methyltransferase N6AMT2 is responsible for Lys79 methylation of human eEF1A, but has been previously documented as a putative N(6)-adenine-specific DNA methyltransferase. It is renamed eEF1A-KMT1 Saccharomyces cerevisiae ? - - 89 2.1.1.244 additional information the methyltransferase N6AMT2 is responsible for Lys79 methylation of human eEF1A, but has been previously documented as a putative N(6)-adenine-specific DNA methyltransferase. It is renamed eEF1A-KMT1 Homo sapiens ? - - 89 2.1.1.244 additional information the substrate recognition motif is M-L/M/K-G/Q. PrmA preferentially methylates free ribosomal protein L11 over an assembled 50S ribosomal subunit Thermus thermophilus ? - - 89 2.1.1.244 additional information YLR285W, also named elongation factor methyltransferase 7 (Efm7), is a dual MTase that installs methyl groups at both N-terminal Gly1 and Lys2 residues of yeast eEF1A protein. Lys2 is methylated only after trimethylation of Gly1. Yeast eEF1A starts with GKEKSHINV and is the only known substrate of Efm7, although there are 35 other yeast proteins with a G-K sequence at their N termini. But Efm7 is not able to methylate the synthetic decamer peptide GKEKSHINVV derived from the N-terminus of eEF1A Saccharomyces cerevisiae ? - - 89 2.1.1.244 additional information analysis of methylation sites, method, detailed overview Saccharomyces cerevisiae ? - - 89 2.1.1.244 additional information analysis of methylation sites, method, detailed overview Homo sapiens ? - - 89 2.1.1.244 additional information histone peptide profiling reveals that human NRMT1is highly selective to human CENP-A and fruit fly H2B, which share a common Xaa-Pro-Lys/Arg motif Homo sapiens ? - - 89 2.1.1.244 additional information motif sequence and signal peptide analyses, and activity-based substrate profiling of NTMT1 utilizing (E)-hex-2-en-5-ynyl-S-adenosyl-L-methionine (Hey-SAM) reveals 72 potential targets, overview Homo sapiens ? - - 89 2.1.1.244 additional information NRMT1 exhibits distributive trimethylase activity in vitro. Isozymes NRMT1 and NRMT2 can interact both in vitro and in vivo, modeling of NRMT1 and NRMT2 interactions. The Ser-Pro-Lys consensus sequence of the RCC1 peptide is a preferred substrate for NRMT1 Homo sapiens ? - - 89 2.1.1.244 additional information NTMT1 is a tri-methyltransferase Homo sapiens ? - - 89 2.1.1.244 additional information the S-adenosyl-L-methionine-dependent protein methyltransferase EFM7 trimethylates the N-terminal glycine Gly-2 of elongation factor 1-alpha (TEF1 and TEF2), before also catalyzing the mono- and dimethylation of Lys-3. The substrate recognition sequence is GKEKSH. Efm7 is not able to methylate the synthetic decamer peptide GKEKSHINVV derived from the N-terminus of eEF1A. Efm7 can methylate domain 1 (residues 1-238) of eEF1A, but to a smaller degree of trimethylation. Although yeast Efm7 is not able to methylate the decamer peptide that is derived from yeast N-terminal eEF1A, METTL13 can methylate the 15mer peptide derived from human N-terminal eEF1A Saccharomyces cerevisiae ? - - 89 2.1.1.244 additional information the substrate recognition motif is A-K-A/G/K Escherichia coli ? - - 89 2.1.1.244 additional information the substrate recognition motif is X-P-K Drosophila melanogaster ? - - 89 2.1.1.244 additional information the substrate recognition motif is X-P-K. YBR261C methylates ribosomal substrates Rp112ab and Rps25a/Rps25b. YBR261C is able to methylate nonamer synthetic peptides, including PPKQQLSKY, which is derived from alpha-N-terminal Rps25a/b and A/S-PKQQLSKY, with Ala or Ser replacing Pro. YBR261C is able to methylate nonamer peptides Saccharomyces cerevisiae ? - - 89 2.1.1.244 additional information the substrate recognition motif is X-P-K/R. NTMT1 is able to methylate hexamer peptides. NTMT1 is known to be a trimethylase that catalyzes mono-, di-, and trimethylation. During the process of multiple methylations, the substrate can be released and rebind to NTMT1, which proceeds through a distributive mechanism for multiple methylations Homo sapiens ? - - 89 2.1.1.244 additional information the substrate recognition sequence is GKEKTH Homo sapiens ? - - 89 2.1.1.244 additional information the substrate recognition motif is M-L/M/K-G/Q. PrmA preferentially methylates free ribosomal protein L11 over an assembled 50S ribosomal subunit Thermus thermophilus DSM 579 ? - - 89 2.1.1.244 additional information the substrate recognition motif is X-P-K. YBR261C methylates ribosomal substrates Rp112ab and Rps25a/Rps25b. YBR261C is able to methylate nonamer synthetic peptides, including PPKQQLSKY, which is derived from alpha-N-terminal Rps25a/b and A/S-PKQQLSKY, with Ala or Ser replacing Pro. YBR261C is able to methylate nonamer peptides Saccharomyces cerevisiae ATCC 204508 ? - - 89 2.1.1.244 additional information the methyltransferase N6AMT2 is responsible for Lys79 methylation of human eEF1A, but has been previously documented as a putative N(6)-adenine-specific DNA methyltransferase. It is renamed eEF1A-KMT1 Saccharomyces cerevisiae ATCC 204508 ? - - 89 2.1.1.244 additional information analysis of methylation sites, method, detailed overview Saccharomyces cerevisiae ATCC 204508 ? - - 89 2.1.1.244 additional information YLR285W, also named elongation factor methyltransferase 7 (Efm7), is a dual MTase that installs methyl groups at both N-terminal Gly1 and Lys2 residues of yeast eEF1A protein. Lys2 is methylated only after trimethylation of Gly1. Yeast eEF1A starts with GKEKSHINV and is the only known substrate of Efm7, although there are 35 other yeast proteins with a G-K sequence at their N termini. But Efm7 is not able to methylate the synthetic decamer peptide GKEKSHINVV derived from the N-terminus of eEF1A Saccharomyces cerevisiae ATCC 204508 ? - - 89 2.1.1.244 additional information the S-adenosyl-L-methionine-dependent protein methyltransferase EFM7 trimethylates the N-terminal glycine Gly-2 of elongation factor 1-alpha (TEF1 and TEF2), before also catalyzing the mono- and dimethylation of Lys-3. The substrate recognition sequence is GKEKSH. Efm7 is not able to methylate the synthetic decamer peptide GKEKSHINVV derived from the N-terminus of eEF1A. Efm7 can methylate domain 1 (residues 1-238) of eEF1A, but to a smaller degree of trimethylation. Although yeast Efm7 is not able to methylate the decamer peptide that is derived from yeast N-terminal eEF1A, METTL13 can methylate the 15mer peptide derived from human N-terminal eEF1A Saccharomyces cerevisiae ATCC 204508 ? - - 89 2.1.1.244 additional information the substrate recognition motif is M-L/M/K-G/Q. PrmA preferentially methylates free ribosomal protein L11 over an assembled 50S ribosomal subunit Thermus thermophilus ATCC 27634 ? - - 89 2.1.1.244 N-terminal L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-methionine - Homo sapiens N-terminal N,N,N-trimethyl-L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine - ? 454427 2.1.1.244 N-terminal L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-methionine - Drosophila melanogaster N-terminal N,N,N-trimethyl-L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine - ? 454427 2.1.1.244 N-terminal L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-methionine - Saccharomyces cerevisiae N-terminal N,N,N-trimethyl-L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine - ? 454427 2.1.1.244 N-terminal L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-methionine - Saccharomyces cerevisiae ATCC 204508 N-terminal N,N,N-trimethyl-L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine - ? 454427 2.1.1.244 S-adenosyl-L-methionine + APKQQLSKY synthetic peptide, modified Rps25a/Rps25b-derived peptide Saccharomyces cerevisiae ? - ? 419047 2.1.1.244 S-adenosyl-L-methionine + APKQQLSKY synthetic peptide, modified yeast protein Rps25a/Rps25b-derived peptide Mus musculus ? - ? 419047 2.1.1.244 S-adenosyl-L-methionine + APKQQLSKY synthetic peptide, modified yeast protein Rps25a/Rps25b-derived peptide Homo sapiens ? - ? 419047 2.1.1.244 S-adenosyl-L-methionine + DPKRIA monomethylation by NTMT1 Homo sapiens S-adenosyl-L-homocysteine + ? - ? 456471 2.1.1.244 S-adenosyl-L-methionine + EPKRIA monomethylation by NTMT1 Homo sapiens S-adenosyl-L-homocysteine + ? - ? 456473 2.1.1.244 S-adenosyl-L-methionine + human histone H3 lower activity with histone H3 compared to histone H4 Homo sapiens S-adenosyl-L-homocysteine + ? - ? 419050 2.1.1.244 S-adenosyl-L-methionine + human histone H4 - Homo sapiens S-adenosyl-L-homocysteine + ? - ? 419051 2.1.1.244 S-adenosyl-L-methionine + N-terminal peptide sequence of a protein all known substrates of NTMT1 contain the N-terminal consensus sequence XPK (X = S/P/A/G), although NTMT1 can also methylate peptides with X being F, Y, C, M, K, R, N, Q, or H in vitro, substrate specificity of NTMT1, overview. Structural basis for the specific N-terminal methylation of a consensus motif, XPK, by NTMT1, overview. Hexapeptides composed of the first six residues of RCC1, i.e. regulator of chromosome condensation 1, are recognized by the enzyme. The first residue within the consensus sequence of the NTMT1 substrates is anchored through a hydrogen bond with the conserved Asn168 of NTMT1 in a spacious binding pocket, which exposes the substrate's reactive alpha-amino group to S-adenosyl-L-methionine in the complex structures, and this very N-terminal residue can tolerate most residue substitutions except the negatively charged residues D and E. Asp180 and His140 can act as bases to facilitate deprotonation of the target alpha-N-terminal amino group. Catalytic reaction proceeds probably involving a SN1 mechanism, overview Homo sapiens S-adenosyl-L-homocysteine + methylated N-terminal peptide sequence of a protein S-adenosyl-L-homocysteine is bound to NTMT1 in an extended conformation. The carboxylate moiety of SAH forms a salt bridge interaction with the highly conserved Arg74, and the ribosyl group stacks with the indole ring of Trp20. In addition, the adenine moiety of SAH is flanked by the hydrophobic side chains of Ile92 and Val137 and interacts with the main chain amide group of Leu119 and the side chain of Gln120 through hydrogen bonding ? 435634 2.1.1.244 S-adenosyl-L-methionine + N-terminal-(A,P,S)PK-[protein] - Homo sapiens S-adenosyl-L-homocysteine + N-terminal-N-methyl-N-(A,P,S)PK-[protein] - ? 454623 2.1.1.244 S-adenosyl-L-methionine + N-terminal-CENP-A human CENP-A histone Homo sapiens S-adenosyl-L-homocysteine + ? - ? 454624 2.1.1.244 S-adenosyl-L-methionine + N-terminal-histone 2B fruit fly histone 2B Homo sapiens S-adenosyl-L-homocysteine + ? - ? 454625 2.1.1.244 S-adenosyl-L-methionine + PPKQQLSKY synthetic peptide, Rps25a/Rps25b-derived peptide Saccharomyces cerevisiae ? - ? 419052 2.1.1.244 S-adenosyl-L-methionine + PPKQQLSKY synthetic peptide, yeast protein Rps25a/Rps25b-derived peptide Mus musculus ? - ? 419052 2.1.1.244 S-adenosyl-L-methionine + PPKQQLSKY synthetic peptide, yeast protein Rps25a/Rps25b-derived peptide Homo sapiens ? - ? 419052 2.1.1.244 S-adenosyl-L-methionine + Ran guanine nucleotide-exchange factor RCC1 NRMT is the predominant alpha-N-methyltransferase for RCC1 Homo sapiens S-adenosyl-L-homocysteine + ? - ? 418066 2.1.1.244 S-adenosyl-L-methionine + Ran guanine nucleotide-exchange factor RCC1 substrate docking and mutational analysis of RCC1 defining the NRMT recognition sequence, the first 3 residues Ser-Pro-Lys interact with NRMT, overview Homo sapiens S-adenosyl-L-homocysteine + ? - ? 418066 2.1.1.244 S-adenosyl-L-methionine + retinoblastoma protein - Homo sapiens S-adenosyl-L-homocysteine + ? - ? 418067 2.1.1.244 S-adenosyl-L-methionine + Rpl12ab methylation of Rpl12ab at the N-terminal proline residue Saccharomyces cerevisiae ? - ? 418068 2.1.1.244 S-adenosyl-L-methionine + Rpl12ab the yeast Rpl12ab protein is dimethylated at the N-terminal proline residue, trimethylated at Lys-3 by Rkm2, and monomethylated at Arg66 Saccharomyces cerevisiae S-adenosyl-L-homocysteine + ? - ? 418069 2.1.1.244 S-adenosyl-L-methionine + Rpl12ab the yeast Rpl12ab protein is dimethylated at the N-terminal proline residue, trimethylated at Lys-3 by Rkm2, and monomethylated at Arg66. Utilization of top down mass spectrometry to determine the sites of methylation of Rpl12ab Saccharomyces cerevisiae S-adenosyl-L-homocysteine + ? - ? 418069 2.1.1.244 S-adenosyl-L-methionine + Rpl12ab the yeast Rpl12ab protein is dimethylated at the N-terminal proline residue, trimethylated at Lys-3 by Rkm2, and monomethylated at Arg66 Saccharomyces cerevisiae BY4742 S-adenosyl-L-homocysteine + ? - ? 418069 2.1.1.244 S-adenosyl-L-methionine + Rpl12ab the yeast Rpl12ab protein is dimethylated at the N-terminal proline residue, trimethylated at Lys-3 by Rkm2, and monomethylated at Arg66. Utilization of top down mass spectrometry to determine the sites of methylation of Rpl12ab Saccharomyces cerevisiae BY4742 S-adenosyl-L-homocysteine + ? - ? 418069 2.1.1.244 S-adenosyl-L-methionine + Rps25a - Saccharomyces cerevisiae ? - ? 418070 2.1.1.244 S-adenosyl-L-methionine + Rps25a Rps25a and Rps25b differ only at position 104, a threonine residue is present in the former and an alanine residue in the latter Saccharomyces cerevisiae ? - ? 418070 2.1.1.244 S-adenosyl-L-methionine + Rps25b - Saccharomyces cerevisiae ? - ? 418071 2.1.1.244 S-adenosyl-L-methionine + Rps25b Rps25a and Rps25b differ only at position 104, a threonine residue is present in the former and an alanine residue in the latter Saccharomyces cerevisiae ? - ? 418071 2.1.1.244 S-adenosyl-L-methionine + SET/TAF-I/PHAPII only the SETalpha splicing variant is a substrate for NRMT, since it begins with the NRMT consensus in contrast to the beta splicing variant Homo sapiens S-adenosyl-L-homocysteine + ? - ? 419053 2.1.1.244 S-adenosyl-L-methionine + SPKQQLSKY synthetic peptide, modified Rps25a/Rps25b-derived peptide Saccharomyces cerevisiae ? - ? 419054 2.1.1.244 S-adenosyl-L-methionine + SPKQQLSKY synthetic peptide, modified yeast protein Rps25a/Rps25b-derived peptide Mus musculus ? - ? 419054 2.1.1.244 S-adenosyl-L-methionine + SPKQQLSKY synthetic peptide, modified yeast protein Rps25a/Rps25b-derived peptide Homo sapiens ? - ? 419054 2.1.1.244 S-adenosyl-L-methionine + SPKRIAKRRSPPADA substrate peptide consisting of the first 15 amino acids of RCC1. NRMT2 V224L is able to significantly decrease the NRMT1 Km with the RCC1 peptide Homo sapiens ? - ? 456617 2.1.1.244 S-adenosyl-L-methionine + SSKRAKAKTTKKRP substrate peptide consisting of the first 14 amino acids of MYL9 Homo sapiens ? - ? 456619