2.1.1.225	additional information	Trm13p belongs to the RFM superfamily of MTases despite the absence of a significant sequence similarity to previously characterized members of this superfamily. The model reveals residues potentially responsible for cofactor binding, tRNA binding, and catalysis of the 2'-O-methylation reaction. The model suggests a presence of one to three disulfide bonds and/or metal ion binding site next to the substrate binding pocket	Saccharomyces cerevisiae	?	89	
2.1.1.225	S-adenosyl-L-methionine + adenosine4 in tRNAHis	-	Saccharomyces cerevisiae	S-adenosyl-L-homocysteine + 2'-O-methyladenosine4 in tRNAHis	413581	
2.1.1.225	S-adenosyl-L-methionine + adenosine4 in tRNAHis	the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA	Saccharomyces cerevisiae	S-adenosyl-L-homocysteine + 2'-O-methyladenosine4 in tRNAHis	413581	
2.1.1.225	S-adenosyl-L-methionine + cytidine4 in tRNAGly(GCC)	-	Saccharomyces cerevisiae	S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAGly(GCC)	413585	
2.1.1.225	S-adenosyl-L-methionine + cytidine4 in tRNAGly(GCC)	the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA	Saccharomyces cerevisiae	S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAGly(GCC)	413585	
2.1.1.225	S-adenosyl-L-methionine + cytidine4 in tRNAPro	-	Saccharomyces cerevisiae	S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAPro	413586	
2.1.1.225	S-adenosyl-L-methionine + cytidine4 in tRNAPro	the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA	Saccharomyces cerevisiae	S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAPro	413586