1.8.4.B3 dabsyl-L-methionine (R)-S-sulfoxide + bovine liver thionein NtMsrB1 Nicotiana tabacum dabsyl-L-methionine + bovine liver thionein + H2O - ? 396957 1.8.4.B3 dabsyl-L-methionine (R)-S-sulfoxide + dithiothreitol NtMsrB1 Nicotiana tabacum dabsyl-L-methionine + dithiothreitol disulfide + H2O - ? 396958 1.8.4.B3 dabsyl-L-methionine (R)-S-sulfoxide + plant Trx-like protein CDSP32 NtMsrB1 Nicotiana tabacum dabsyl-L-methionine + ? - ? 396959 1.8.4.B3 dabsyl-L-methionine (R)-S-sulfoxide + selenocysteamine NtMsrB1 Nicotiana tabacum dabsyl-L-methionine + 2,2'-disulfanediyldiethanamine + H2O - ? 396960 1.8.4.B3 L-methionine (R)-S-sulfoxide + dithiothreitol - Homo sapiens L-methionine + dithiothreitol disulfide + H2O - ? 397632 1.8.4.B3 L-methionine (R)-S-sulfoxide + thionein - Homo sapiens L-methionine + thionein disulfide + H2O - ? 397633 1.8.4.B3 L-methionine (R)-S-sulfoxide + thioredoxin reduced thioredoxin is not an efficient reducing agent for hMsrB3. Less than 5% of the activity with dithiothreitol Homo sapiens L-methionine + thioredoxin disulfide - ? 397634 1.8.4.B3 L-methionine (R)-S-sulfoxide + thioredoxin reduced thioredoxin is not an efficient reducing agent for hMsrB2. Less than 5% of the activity with dithiothreitol Homo sapiens L-methionine + thioredoxin disulfide + H2O - ? 397635 1.8.4.B3 L-methionine (R)-sulfoxide + dithiothreitol - Homo sapiens L-methionine + dithiothreitol disulfide - ? 377057 1.8.4.B3 additional information NtMsrB1 shows very little activity towards L-methionine (R)-sulfoxide. Escherichia coli thioredoxin can not serve as a reducing agent for NtMsrB1. The Trx-independent MsrB enzyme lacks an additional cysteine (resolving cysteine) that is capable of forming a disulfide bond on the enzyme during the catalytic reaction Nicotiana tabacum ? - ? 89 1.8.4.B3 additional information zinc-containing metallothionein in the presence of EDTA can serve as a reducing agent. Thioredoxin can reduce oxidized thionein Homo sapiens ? - ? 89 1.8.4.B3 peptide-L-methionine-(R)-S-sulfoxide + thionein - Nicotiana tabacum peptide-L-methionine + thionein disulfide + H2O - ? 394948