1.4.1.B4 carboxynorspermidine + NAD+ + H2O NADH is a much poorer cofactor than NADPH Vibrio alginolyticus L-aspartic 4-semialdehyde + 1,3-diaminopropane + NADH + H+ - 414410 1.4.1.B4 carboxynorspermidine + NAD+ + H2O NADH is a much poorer cofactor than NADPH Vibrio alginolyticus ATCC 17749 L-aspartic 4-semialdehyde + 1,3-diaminopropane + NADH + H+ - 414410 1.4.1.B4 carboxynorspermidine + NADP+ + H2O i.e. (3-((3-aminopropyl)amino)propyl)carbamate, incubation of the purified enzyme in the range of pH 6-8, with cofactor NADP+ or NAD+ concentrations up to 5 mM and substrate concentrations of carboxynorspermidine up to 10 mM, no reversible activity is observed Vibrio alginolyticus L-aspartic 4-semialdehyde + 1,3-diaminopropane + NADPH + H+ i.e. (2S)-2-amino-4-oxobutanoate 414411 1.4.1.B4 carboxynorspermidine + NADP+ + H2O i.e. (3-((3-aminopropyl)amino)propyl)carbamate, incubation of the purified enzyme in the range of pH 6-8, with cofactor NADP+ or NAD+ concentrations up to 5 mM and substrate concentrations of carboxynorspermidine up to 10 mM, no reversible activity is observed Vibrio alginolyticus ATCC 17749 L-aspartic 4-semialdehyde + 1,3-diaminopropane + NADPH + H+ i.e. (2S)-2-amino-4-oxobutanoate 414411 1.4.1.B4 carboxyspermidine + NADP+ + H2O i.e. 3-[(4-aminobutyl)amino]-L-alanine, slightly active with putrescine, ca. 7% of the rate compared to 1,3-diaminopropane Vibrio alginolyticus L-aspartic 4-semialdehyde + putrescine + NADPH + H+ - 414412 1.4.1.B4 carboxyspermidine + NADP+ + H2O i.e. 3-[(4-aminobutyl)amino]-L-alanine, slightly active with putrescine, ca. 7% of the rate compared to 1,3-diaminopropane Vibrio alginolyticus ATCC 17749 L-aspartic 4-semialdehyde + putrescine + NADPH + H+ - 414412 1.4.1.B4 L-aspartic 4-semialdehyde + 1,3-diaminopropane + NADPH + H+ - Campylobacter jejuni carboxynorspermidine + NADP+ + H2O - 424852 1.4.1.B4 additional information no activity with ethylenediamine, cadaverine, and D-aspartic beta-semialdehyde Vibrio alginolyticus ? - 89 1.4.1.B4 additional information no activity with ethylenediamine, cadaverine, and D-aspartic beta-semialdehyde Vibrio alginolyticus ATCC 17749 ? - 89