1.3.7.12 additional information barley RCCR produces the C1 isomer pFCC-1 Hordeum vulgare ? - ? 89 1.3.7.12 additional information cell death gene ACD2 encodes red chlorophyll catabolite reductase and suppresses the spread of disease symptoms Arabidopsis sp. ? - ? 89 1.3.7.12 additional information RCCR absence causes leaf cell death as a result of the accumulation of photodynamic RCC. RCCR (together with pheophorbide a oxygenase) is required for the detoxification of chlorophyll catabolites Arabidopsis thaliana ? - ? 89 1.3.7.12 additional information the enzyme is involved in breakdown of chlorophyll Brassica napus ? - ? 89 1.3.7.12 additional information the enzyme is involved in chlorophyll breakdown Hordeum vulgare ? - ? 89 1.3.7.12 additional information the enzyme is involved in chlorophyll breakdown in senescent Arabidopsis leaves Arabidopsis thaliana ? - ? 89 1.3.7.12 additional information the major product of reduction of red chlorophyll catabolite is pFCC1, but small quantities of its C1 epimer, pFCC-2, also accumulate. Red chlorophyll catabolite reductase and pheophorbide a oxygenase catalyse the key eaction of chlorophyll catabolism, porphin macrocycle cleavage of pheide a to a primary fluorescent catabolite Hordeum vulgare ? - ? 89 1.3.7.12 additional information the major product of reduction of red chlorophyll catabolite is pFCC1, but small quantities of its C1 epimer, pFCC-2, also accumulate. Red chlorophyll catabolite reductase and pheophorbide a oxygenase catalyse the key reaction of chlorophyll catabolism, porphin macrocycle cleavage of pheide a to a primary fluorescent catabolite Arabidopsis thaliana ? - ? 89 1.3.7.12 additional information with Arabidopsis RCCR, the C1 isomer pFCC-1 is formed. RCCR could be required to mediate an efficient interaction between red chlorophyll catabolite (still bound to pheophorbide a oxygenase) and ferredoxin, thereby enabling a fast, regio-, and stereoselective reduction to blue-fluorescing intermediate Arabidopsis sp. ? - ? 89 1.3.7.12 additional information with tomato RCCR, the C1 isomer pFCC-2 is formed Solanum lycopersicum ? - ? 89 1.3.7.12 additional information spinach RCCR produces the C1 isomer pFCC-2 Spinacia oleracea ? - ? 89 1.3.7.12 additional information strongly interacts with catabolic enzymes (CCEs), NONYELLOW COLORING1 (NYC1), pheophorbide a oxygenase (PAO), NYC1-LIKE (NOL) and pheophytinase Arabidopsis thaliana ? - ? 89 1.3.7.12 additional information in pFCC-1 of Brassica napus as well as in Chlorella protothecoides 18O is only found in the formyl group of pyrrole B, and hence the respective enzymes are monooxygenases. The lactam oxygen in pyrrole A is most probably derived from H2O Brassica napus ? - ? 89 1.3.7.12 additional information in pFCC-1 of Brassica napus as well as in Chlorella protothecoides 18O is only found in the formyl group of pyrrole B, and the respective enzymes are monooxygenases. The lactam oxygen in pyrrole A is most probably derived from H2O Auxenochlorella protothecoides ? - ? 89 1.3.7.12 additional information the enzyme interacts with the 7-hydroxymethyl chlorophyll a reductase, HCAR, in in yeast two-hybrid assay and in Arabidopsis thaliana chlorophyll catabolism Arabidopsis thaliana ? - ? 89 1.3.7.12 additional information when heterologous combinations of PaO and RCCR are tested, the type of primary fluorescent chlorophyll catabolite turns out to be invariably determined by the source of RCCR, i.e. the slightly more polar pFCC-1 or the less polar pFCC-2 Spinacia oleracea ? - ? 89 1.3.7.12 additional information when heterologous combinations of PaO and RCCR are tested, the type of primary fluorescent chlorophyll catabolite turns out to be invariably determined by the source of RCCR, i.e. the slightly more polar pFCC-1 or the less polar pFCC-2 Selaginella sp. ? - ? 89 1.3.7.12 additional information when heterologous combinations of PaO and RCCR are tested, the type of primary fluorescent chlorophyll catabolite turns out to be invariably determined by the source of RCCR, i.e. the slightly more polar pFCC-1 or the less polar pFCC-2 Tropaeolum majus ? - ? 89 1.3.7.12 additional information when heterologous combinations of PaO and RCCR are tested, the type of primary fluorescent chlorophyll catabolite turns out to be invariably determined by the source of RCCR, i.e. the slightly more polar pFCC-1 or the less polar pFCC-2 Auxenochlorella protothecoides ? - ? 89 1.3.7.12 additional information when heterologous combinations of PaO and RCCR are tested, the type of primary fluorescent chlorophyll catabolite turns out to be invariably determined by the source of RCCR, i.e. the slightly more polar pFCC-1 or the less polar pFCC-2 Ginkgo biloba ? - ? 89 1.3.7.12 additional information when heterologous combinations of PaO and RCCR are tested, the type of primary fluorescent chlorophyll catabolite turns out to be invariably determined by the source of RCCR, i.e. the slightly more polar pFCC-1 or the less polar pFCC-2 Taxus baccata ? - ? 89 1.3.7.12 additional information when heterologous combinations of PaO and RCCR are tested, the type of primary fluorescent chlorophyll catabolite turns out to be invariably determined by the source of RCCR, i.e. the slightly more polar pFCC-1 or the less polar pFCC-2 Equisetum sp. ? - ? 89 1.3.7.12 additional information when heterologous combinations of PaO and RCCR are tested, the type of primary fluorescent chlorophyll catabolite turns out to be invariably determined by the source of RCCR, i.e. the slightly more polar pFCC-1 or the less polar pFCC-2 Cycas sp. ? - ? 89 1.3.7.12 additional information when heterologous combinations of PaO and RCCR are tested, the type of primary fluorescent chlorophyll catabolite turns out to be invariably determined by the source of RCCR, i.e. the slightly more polar pFCC-1 or the less polar pFCC-2 Cleome graveolens ? - ? 89 1.3.7.12 additional information when heterologous combinations of PaO and RCCR are tested, the type of primary fluorescent chlorophyll catabolite turns out to be invariably determined by the source of RCCR, i.e. the slightly more polar pFCC-1 or the less polar pFCC-2 Hordeum vulgare ? - ? 89 1.3.7.12 additional information when heterologous combinations of PaO and RCCR are tested, the type of primary fluorescent chlorophyll catabolite turns out to be invariably determined by the source of RCCR, i.e. the slightly more polar pFCC-1 or the less polar pFCC-2 Psilotum ? - ? 89 1.3.7.12 additional information when heterologous combinations of PaO and RCCR are tested, the type of primary fluorescent chlorophyll catabolite turns out to be invariably determined by the source of RCCR, i.e. the slightly more polar pFCC-1 or the less polar pFCC-2 Angiopteris ? - ? 89 1.3.7.12 additional information when heterologous combinations of PaO and RCCR are tested, the type of primary fluorescent chlorophyll catabolite turns out to be invariably determined by the source of RCCR, i.e. the slightly more polar pFCC-1 or the less polar pFCC-2 Metasequoia ? - ? 89 1.3.7.12 additional information when heterologous combinations of PaO and RCCR are tested, the type of primary fluorescent chlorophyll catabolite turns out to be invariably determined by the source of RCCR, i.e. the slightly more polar pFCC-1 or the less polar pFCC-2 Euptelea ? - ? 89 1.3.7.12 additional information when heterologous combinations of PaO and RCCR are tested, the type of primary fluorescent chlorophyll catabolite turns out to be invariably determined by the source of RCCR, i.e. the slightly more polar pFCC-1 or the less polar pFCC-2 Picea ? - ? 89 1.3.7.12 primary fluorescent chlorophyll catabolite + NADP+ stereospecific reaction Arabidopsis thaliana red chlorophyll catabolite + NADPH + H+ red chlorophyll catabolite, RCC, binding does not drastically change the RCCR structure, binding structure and mechanism analysis, overview. Comparison of the RCC-binding pockets of wild-type RCCRDELTA49 and F218V RCCRDELTA49, overview r 413541 1.3.7.12 primary fluorescent chlorophyll catabolite + NADP+ stereospecific reaction. RCCR catalyzes the ferredoxin-dependent and site-specific reduction of the C20/C1 double bond of red chlorophyll catabolite, RCC, the catabolic intermediate produced in chlorophyll degradation Arabidopsis thaliana red chlorophyll catabolite + NADPH + H+ - r 413541 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ - Phaseolus vulgaris primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster - ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ - Spinacia oleracea primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster - ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ - Capsicum annuum primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster - ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ - Selaginella sp. primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster - ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ - Tropaeolum majus primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster - ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ - Auxenochlorella protothecoides primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster - ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ - Ginkgo biloba primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster - ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ - Taxus baccata primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster - ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ - Parachlorella kessleri primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster - ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ - Equisetum sp. primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster - ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ - Cycas sp. primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster - ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ - Cleome graveolens primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster - ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ - Arabidopsis thaliana primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster - ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ - Festuca pratensis primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster - ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ - Hordeum vulgare primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster - ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ - Brassica napus primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster - ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ - Lolium temulentum primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster - ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ - Psilotum primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster - ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ - Angiopteris primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster - ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ - Metasequoia primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster - ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ - Euptelea primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster - ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ - Picea primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster - ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ - Tropaeolum majus primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster catabolite pFCC-0, possible representing a modified version of either pFCC-1 or -2 ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ - Cleome graveolens primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster catabolite pFCC-0, possible representing a modified version of either pFCC-1 or -2 ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ - Selaginella sp. primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster catabolite pFCC-3 ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ - Taxus baccata primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster catabolite pFCC-3 ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ - Equisetum sp. primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster catabolite pFCC-3 ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ - Cycas sp. primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster catabolite pFCC-3 ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ - Psilotum primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster catabolite pFCC-3 ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ - Angiopteris primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster catabolite pFCC-3 ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ - Arabidopsis thaliana primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster formation of a stereospecific product, overview ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ - Capsicum annuum primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster i.e. Ca-pFCC-2, or 1-epi-FCC, or (1zeta,132R,17S,18S)-31,32-didehydro-1,4,5,10,17,18,20,22-octahydro-132-(methoxycarbonyl)-4,5-dioxo-4,5-secophytoporphyrin ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ - Brassica napus primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster product epimer Bn-FCC-2 ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ in Chlorella, the release of red pigments is correlated with the loss of chlorophyll only if the cells are kept in the dark. These pigments are neither produced in light-grown cells nor in the dark if a source of nitrogen is provided Auxenochlorella protothecoides primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster - ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ in Chlorella, the release of red pigments is correlated with the loss of chlorophyll only if the cells are kept in the dark. These pigments are neither produced in light-grown cells nor in the dark if a source of nitrogen is provided Parachlorella kessleri primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster - ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ two atoms of oxygen are introduced into RCC, pFCC-1 and the corresponding red catabolites of Chlorella protothecoides and production of pFCC-1 from Pheide a requires dioxygen Brassica napus primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster - ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ two atoms of oxygen are introduced into RCC, pFCC-1 and the corresponding red catabolites of Chlorella protothecoides and production of pFCC-1 from Pheide a requires dioxygen Auxenochlorella protothecoides primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster - ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ a red tetrapyrrole Arabidopsis thaliana primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster two different fluorescent chlorophyll catabolites are formed from red chlorophyll catabolite and identified as primary fluorescent chlorophyll catabolite and its C1 epimer, 1-epi-pFCC ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ a red tetrapyrrole Brassica napus primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster two different fluorescent chlorophyll catabolites are formed from red chlorophyll catabolite and identified as primary fluorescent chlorophyll catabolite and its C1 epimer, 1-epi-pFCC ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ loose substrate binding allows for conformation change during the reaction, stereospcific reaction, mechanism, overview Arabidopsis thaliana primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster formation of a stereospecific product, overview ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ reduction of the C20/C1 double bond of red chlorophyll catabolite is catalyzed by RCCR in a stereospecific manner forming the C1 isomer pFCC-1 Arabidopsis thaliana primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster - ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ stereospecificity towards reduction of C1 Phaseolus vulgaris primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster - ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ stereospecificity towards reduction of C1 Festuca pratensis primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster - ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ stereospecificity towards reduction of C1 Hordeum vulgare primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster three products identified as pFCC-1 and pFCC-2, that have identical constitutions but differ in the absolute configuration at C1, and pFCC-3 with undetermined structure ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ stereospecificity towards reduction of C1 Capsicum annuum primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster two products identified as pFCC-1 and pFCC-2, that have identical constitutions but differ in the absolute configuration at C1 ? 434734 1.3.7.12 red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ two atoms of oxygen are introduced into RCC, pFCC-1 and the corresponding red catabolites of Chlorella protothecoides and production of pFCC-1 from Pheide a requires dioxygen, stereospecificity towards reduction of C1 Brassica napus primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster - ? 434734 1.3.7.12 red chlorophyll catabolite + reduced acceptor three different primary fluorescent chlorophyll catabolites are produced, two of which could be identified as the stereoisomeric pFCCs from canola (Brassica napus) (pFCC-1) and sweet pepper (Capsicum annuum) (pFCC-2), respectively Hordeum vulgare primary fluorescent chlorophyll catabolite + acceptor - ? 389646 1.3.7.12 red chlorophyll catabolite + reduced acceptor chlorophyll catabolism, leaf senescence, ring-opening activity, a reduction destroys the residual conjugated bond system to yield the colourless product Arabidopsis thaliana primary fluorescent chlorophyll catabolite + oxidized acceptor pFCC, the fate of primary fluorescent chlorophyll catabolite is to be conjugated, imported into the vacuole and tautomerized to accumulate there as non-fluorescent chlorophyll catabolites and possibly other terminal catabolites ? 402904 1.3.7.12 red chlorophyll catabolite + reduced acceptor the key steps in the degradation pathway of chlorophylls are the ring-opening reaction catalyzed by pheophorbide a oxygenase and sequential reduction by RCCR, RCCR catalyzes the ferredoxin-dependent reduction of the C20/C1 double bond of red chlorophyll catabolite Arabidopsis thaliana primary fluorescent chlorophyll catabolite + oxidized acceptor in the acidic environment of vacuoles, primary fluorescent chlorophyll catabolite is spontaneously converted into nonfluorescent chlorophyll catabolites ? 402904