1.3.1.14	(S)-dihydroorotate + 2,6-dichlorophenolindophenol	-	Lactococcus lactis	orotate + reduced 2,6-dichlorophenolindophenol	-	?	395167	
1.3.1.14	(S)-dihydroorotate + acceptor	different specific activities with potassium ferricyanide, O2, fumarate and NAD+ as electron acceptors for PyrDI and the holoenzyme	Bacillus subtilis	orotate + reduced acceptor	-	?	258663	
1.3.1.14	(S)-dihydroorotate + acceptor	fourth step in UMP-biosynthesis	Bacillus subtilis	orotate + reduced acceptor	-	?	258663	
1.3.1.14	(S)-dihydroorotate + NAD+	-	Lactococcus lactis	orotate + NADH + H+	-	r	258349	
1.3.1.14	(S)-dihydroorotate + NAD+	-	Faecalicatena orotica	orotate + NADH + H+	-	?	258349	
1.3.1.14	(S)-dihydroorotate + NAD+	-	Faecalicatena orotica	orotate + NADH + H+	-	r	258349	
1.3.1.14	(S)-dihydroorotate + NAD+	-	Lactococcus lactis	orotate + NADH + H+	-	?	258349	
1.3.1.14	(S)-dihydroorotate + NAD+	anti-elimination of hydrogen from (S)-dihydroorotate	Faecalicatena orotica	orotate + NADH + H+	-	r	258349	
1.3.1.14	(S)-dihydroorotate + NAD+	equilibrium favours direction of orotate reduction	Faecalicatena orotica	orotate + NADH + H+	-	?	258349	
1.3.1.14	(S)-dihydroorotate + NAD+	equilibrium favours direction of orotate reduction	Faecalicatena orotica	orotate + NADH + H+	-	r	258349	
1.3.1.14	(S)-dihydroorotate + NAD+	equilibrium favours direction of orotate reduction	Faecalicatena orotica	orotate + NADH + H+	orotate is identical with 4-carboxyuracil	r	258349	
1.3.1.14	(S)-dihydroorotate + NAD+	mechanism of dehydrogenation, mechanism of electron transfer: L-dihydroorotate transfers a pair of electrons to FMN via iron-sulfur cluster via FAD to NAD+	Lactococcus lactis	orotate + NADH + H+	-	?	258349	
1.3.1.14	(S)-dihydroorotate + NAD+	mechanism of dehydrogenation, mechanism of electron transfer: L-dihydroorotate transfers a pair of electrons to FMN via iron-sulfur cluster via FAD to NAD+	Lactococcus lactis	orotate + NADH + H+	-	r	258349	
1.3.1.14	(S)-dihydroorotate + NAD+	NAD+ as ultimate electron acceptor	Bacillus subtilis	orotate + NADH + H+	-	?	258349	
1.3.1.14	(S)-dihydroorotate + NAD+	NAD+ as ultimate electron acceptor	Lactococcus lactis	orotate + NADH + H+	-	?	258349	
1.3.1.14	(S)-dihydroorotate + NAD+	NAD+ as ultimate electron acceptor	Lactococcus lactis	orotate + NADH + H+	-	r	258349	
1.3.1.14	(S)-dihydroorotate + NAD+	NAD+ as ultimate electron acceptor	Enterococcus faecalis	orotate + NADH + H+	-	?	258349	
1.3.1.14	(S)-dihydroorotate + NAD+	NAD+ as ultimate electron acceptor	Faecalicatena orotica	orotate + NADH + H+	-	?	258349	
1.3.1.14	(S)-dihydroorotate + NAD+	NAD+ as ultimate electron acceptor	Faecalicatena orotica	orotate + NADH + H+	-	r	258349	
1.3.1.14	(S)-dihydroorotate + NAD+	substrate is oxidized by NAD+ and oxygen	Faecalicatena orotica	orotate + NADH + H+	-	r	258349	
1.3.1.14	(S)-dihydroorotate + NAD+	substrate is oxidized by NAD+ and oxygen	Faecalicatena orotica	orotate + NADH + H+	lipoic acid is no substitute for orotate	?	258349	
1.3.1.14	(S)-dihydroorotate + NAD+	at higher pH values reaction favours direction of dihydroorotate oxidation, whereas at lower pH values direction of orotate reduction is favoured	Lactococcus lactis	orotate + NADH + H+	-	r	258349	
1.3.1.14	(S)-dihydroorotate + NAD+	acceptor: NAD+	Enterococcus faecalis	orotate + NADH + H+	-	r	258349	
1.3.1.14	(S)-dihydroorotate + NAD+	mechanism and pH-dependence of reaction	Enterococcus faecalis	orotate + NADH + H+	-	r	258349	
1.3.1.14	5-fluorodihydroorotate + NAD+	more active substrate for reverse reaction than orotate	Faecalicatena orotica	5-fluoroorotate + NADH + H+	-	r	258350	
1.3.1.14	dihydroorotate + acceptor	acceptor: NAD+	Bacillus subtilis	orotate + reduced acceptor	-	?	258769	
1.3.1.14	dihydroorotate + acceptor	acceptor: menadione	Bacillus subtilis	orotate + reduced acceptor	-	?	258769	
1.3.1.14	dihydroorotate + acceptor	activity measurement in permeabilized Ehrlich ascites tumor cells, acceptor: nitroblue tetrazolium	Mus musculus	orotate + reduced acceptor	-	?	258769	
1.3.1.14	dihydroorotate + acceptor	acceptor: NAD+ for pyrDa gene product, fumarate for pyrDb gene product	Lactococcus lactis	orotate + reduced acceptor	-	?	258769	
1.3.1.14	dihydroorotate + NAD+	-	Lactococcus lactis	orotate + NADH + H+	-	?	370137	
1.3.1.14	dihydroorotate + NAD+	-	Faecalicatena orotica	orotate + NADH + H+	breakdown of orotate	r	370137	
1.3.1.14	dihydroorotate + NAD+	fourth step and sole redox reaction in the pyrimidine de novo biosynthetic pathway	Lactococcus lactis	orotate + NADH + H+	-	r	370137	
1.3.1.14	dihydroorotate + NAD+	fourth step and sole redox reaction in the pyrimidine de novo biosynthetic pathway	Enterococcus faecalis	orotate + NADH + H+	-	r	370137	
1.3.1.14	dihydroorotate + NAD+	fourth step and sole redox reaction in the pyrimidine de novo biosynthetic pathway	Faecalicatena orotica	orotate + NADH + H+	-	r	370137	
1.3.1.14	dihydroorotate + NAD+	biosynthesis of pyrimidines	Faecalicatena orotica	orotate + NADH + H+	-	?	370137	
1.3.1.14	dihydroorotate + NAD+	enzyme presumably functions mainly in the metabolic synthesis of pyrimidines	Faecalicatena orotica	orotate + NADH + H+	-	r	370137	
1.3.1.14	additional information	-	Faecalicatena orotica	?	-	?	89	
1.3.1.14	additional information	NAD+ binding domain in the beta subunit of enzyme, Cys-135 plays a catalytic role and Lys-48 is important for orienting the substrate in the active site and is able to interact with FMN	Lactococcus lactis	?	-	?	89	
1.3.1.14	additional information	not: uracil, cytosine, 5-methylcytosine, thymine as substrates	Faecalicatena orotica	?	-	?	89	
1.3.1.14	additional information	3-acetylpyridine-NAD+ is a more effective oxidant for dihydroorotate than NAD+, methylene blue can serve as oxidant, but not cytochrome c	Faecalicatena orotica	?	-	?	89	
1.3.1.14	additional information	O2, fumarate, and NADP+ do not serve as electron acceptors	Lactococcus lactis	?	-	?	89