1.3.1.12 L-arogenate + NAD+ - Escherichia coli L-tyrosine + NADH + CO2 - ? 258346 1.3.1.12 L-arogenate + NAD+ - Klebsiella pneumoniae L-tyrosine + NADH + CO2 - ? 258346 1.3.1.12 L-arogenate + NAD+ - Aquifex aeolicus L-tyrosine + NADH + CO2 - ? 258346 1.3.1.12 additional information a dimeric enzyme, with each monomer consisting of an N-terminal alpha/beta dinucleotide-binding domain and a C-terminal alpha-helical dimerization domain. Absence of an alpha/beta motif in HinfPDH that is present in other TyrA proteins. Residues from this motif are involved in discrimination between NADP+ and NAD+. The loop between beta5 and beta6 in the N-terminal domain is much shorter in HinfPDH and an extra helix is present at the C-terminus. Furthermore, HinfPDH adopts a more closed conformation compared with TyrA proteins that do not have tyrosine bound. This conformational change brings the substrate, cofactor and active-site residues into close proximity for catalysis. An ionic network consisting of Arg297, a key residue for tyrosine binding, a water molecule, Asp206, from the loop between beta5 and beta6, and Arg365', from the additional C-terminal helix of the adjacent monomer, is observed that might be involved in gating the active site. Active site structure, overview Haemophilus influenzae ? - ? 89 1.3.1.12 additional information no activity with arogenate Saccharomyces cerevisiae ? - ? 89 1.3.1.12 additional information no activity with arogenate Pleurotus ostreatus ? - ? 89 1.3.1.12 additional information a dimeric enzyme, with each monomer consisting of an N-terminal alpha/beta dinucleotide-binding domain and a C-terminal alpha-helical dimerization domain. Absence of an alpha/beta motif in HinfPDH that is present in other TyrA proteins. Residues from this motif are involved in discrimination between NADP+ and NAD+. The loop between beta5 and beta6 in the N-terminal domain is much shorter in HinfPDH and an extra helix is present at the C-terminus. Furthermore, HinfPDH adopts a more closed conformation compared with TyrA proteins that do not have tyrosine bound. This conformational change brings the substrate, cofactor and active-site residues into close proximity for catalysis. An ionic network consisting of Arg297, a key residue for tyrosine binding, a water molecule, Asp206, from the loop between beta5 and beta6, and Arg365', from the additional C-terminal helix of the adjacent monomer, is observed that might be involved in gating the active site. Active site structure, overview Haemophilus influenzae KW20 ? - ? 89 1.3.1.12 prephenate + NAD+ - Bacillus subtilis 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ - Escherichia coli 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ - Saccharomyces cerevisiae 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ - Klebsiella aerogenes 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ - Pseudomonas aeruginosa 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ - Neisseria gonorrhoeae 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ - Klebsiella pneumoniae 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ - Corynebacterium glutamicum 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ - Mycobacterium tuberculosis 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ - Cupriavidus necator 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ - Sphingomonas paucimobilis 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ - Zymomonas mobilis 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ - Xanthomonas campestris 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ - Neurospora sp. 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ - Acholeplasma laidlawii 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ - Salmonella sp. 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ - Candida maltosa 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ - Novosphingobium capsulatum 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ - Mycoplasmopsis gallinarum 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ - Aquifex aeolicus 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ - Microtetraspora glauca 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ - Pseudomonas flexibilis 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ - Azomonas insignis 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ - Mycoplasma iowae 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ - Methanohalophilus mahii 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ calorimetric and equilibrium measurements Pantoea agglomerans 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ mechanism, kinetic studies Escherichia coli 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ mechanism, kinetic studies Escherichia coli 4-hydroxyphenylpyruvate + NADH + CO2 - ir 258345 1.3.1.12 prephenate + NAD+ mechanism, kinetic studies Klebsiella aerogenes 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ mechanism, kinetic studies Klebsiella aerogenes 4-hydroxyphenylpyruvate + NADH + CO2 - ir 258345 1.3.1.12 prephenate + NAD+ biosynthesis of L-tyrosine Bacillus subtilis 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ biosynthesis of L-tyrosine Escherichia coli 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ biosynthesis of L-tyrosine Escherichia coli 4-hydroxyphenylpyruvate + NADH + CO2 - ir 258345 1.3.1.12 prephenate + NAD+ biosynthesis of L-tyrosine Saccharomyces cerevisiae 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ biosynthesis of L-tyrosine Klebsiella aerogenes 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ biosynthesis of L-tyrosine Klebsiella aerogenes 4-hydroxyphenylpyruvate + NADH + CO2 - ir 258345 1.3.1.12 prephenate + NAD+ biosynthesis of L-tyrosine Pseudomonas aeruginosa 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ biosynthesis of L-tyrosine Neisseria gonorrhoeae 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ biosynthesis of L-tyrosine Klebsiella pneumoniae 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ biosynthesis of L-tyrosine Corynebacterium glutamicum 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ biosynthesis of L-tyrosine Pantoea agglomerans 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ biosynthesis of L-tyrosine Cupriavidus necator 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ biosynthesis of L-tyrosine Sphingomonas paucimobilis 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ biosynthesis of L-tyrosine Xanthomonas campestris 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ biosynthesis of L-tyrosine Neurospora sp. 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ biosynthesis of L-tyrosine Acholeplasma laidlawii 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ biosynthesis of L-tyrosine Salmonella sp. 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ biosynthesis of L-tyrosine Candida maltosa 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ biosynthesis of L-tyrosine Novosphingobium capsulatum 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ biosynthesis of L-tyrosine Mycoplasmopsis gallinarum 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ biosynthesis of L-tyrosine Microtetraspora glauca 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ biosynthesis of L-tyrosine Pseudomonas flexibilis 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ biosynthesis of L-tyrosine Azomonas insignis 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ biosynthesis of L-tyrosine Mycoplasma iowae 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ - Acholeplasma laidlawii JA1 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ biosynthesis of L-tyrosine Acholeplasma laidlawii JA1 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ - Escherichia coli K-12 MG1655 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ - Mycobacterium tuberculosis H37Rv 4-hydroxyphenylpyruvate + NADH + CO2 - ? 258345 1.3.1.12 prephenate + NAD+ - Haemophilus influenzae 4-hydroxyphenylpyruvate + CO2 + NADH - ? 371124 1.3.1.12 prephenate + NAD+ - Escherichia coli 4-hydroxyphenylpyruvate + CO2 + NADH - ? 371124 1.3.1.12 prephenate + NAD+ - Saccharomyces cerevisiae 4-hydroxyphenylpyruvate + CO2 + NADH - ? 371124 1.3.1.12 prephenate + NAD+ - Aquifex aeolicus 4-hydroxyphenylpyruvate + CO2 + NADH - ? 371124 1.3.1.12 prephenate + NAD+ - Pleurotus ostreatus 4-hydroxyphenylpyruvate + CO2 + NADH - ? 371124 1.3.1.12 prephenate + NAD+ second step in the biosynthesis of L-tyrosine Escherichia coli 4-hydroxyphenylpyruvate + CO2 + NADH - ? 371124 1.3.1.12 prephenate + NAD+ the enzyme is involved in aromatic amino acid biosynthesis Archaeoglobus fulgidus 4-hydroxyphenylpyruvate + CO2 + NADH - ? 371124 1.3.1.12 prephenate + NAD+ activity with NADP+ as coenzyme is about 10% of that with NAD+, suggesting that NAD+ is likely the preferred and physiological coenzyme Archaeoglobus fulgidus 4-hydroxyphenylpyruvate + CO2 + NADH - ? 371124 1.3.1.12 prephenate + NAD+ - Haemophilus influenzae RD KW20 4-hydroxyphenylpyruvate + CO2 + NADH - ? 371124 1.3.1.12 prephenate + NAD+ - Haemophilus influenzae 4-hydroxyphenylpyruvate + CO2 + NADH + H+ - ? 413540 1.3.1.12 prephenate + NAD+ - Bacillus anthracis 4-hydroxyphenylpyruvate + CO2 + NADH + H+ - ? 413540 1.3.1.12 prephenate + NAD+ key active-site residues are located at the domain interface, including His200, Arg297 and Ser179, that are involved in catalysis and/or ligand binding and are highly conserved in TyrA proteins Haemophilus influenzae 4-hydroxyphenylpyruvate + CO2 + NADH + H+ - ? 413540 1.3.1.12 prephenate + NAD+ - Haemophilus influenzae KW20 4-hydroxyphenylpyruvate + CO2 + NADH + H+ - ? 413540 1.3.1.12 prephenate + NAD+ key active-site residues are located at the domain interface, including His200, Arg297 and Ser179, that are involved in catalysis and/or ligand binding and are highly conserved in TyrA proteins Haemophilus influenzae KW20 4-hydroxyphenylpyruvate + CO2 + NADH + H+ - ? 413540 1.3.1.12 prephenate + NAD+ - uncultured bacterium 4-hydroxyphenylpyruvate + NADH + H+ + CO2 - ? 424969 1.3.1.12 prephenate + NAD+ - Streptococcus mutans 4-hydroxyphenylpyruvate + NADH + H+ + CO2 - ? 424969 1.3.1.12 prephenate + NAD+ - Streptococcus mutans ATCC 700610 4-hydroxyphenylpyruvate + NADH + H+ + CO2 - ? 424969 1.3.1.12 prephenate + NADP+ - Methanohalophilus mahii 4-hydroxyphenylpyruvate + NADPH + CO2 - ? 258347 1.3.1.12 prephenate + NADP+ very low activity Saccharomyces cerevisiae 4-hydroxyphenylpyruvate + CO2 + NADPH - ? 258348 1.3.1.12 prephenate + NADP+ weak activity Pleurotus ostreatus 4-hydroxyphenylpyruvate + CO2 + NADPH - ? 258348