1.14.19.25	a dihomo-gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+	(8Z,11Z,14Z)-icosa-8,11,14-trienoyl-[glycerolipid] i.e. dihomo-linolenoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases	Mortierella alpina	a (8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O	-	?	431449	
1.14.19.25	a dihomo-gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+	(8Z,11Z,14Z)-icosa-8,11,14-trienoyl-[glycerolipid] i.e. dihomo-linolenoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases	Mortierella alpina 1S-4	a (8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O	-	?	431449	
1.14.19.25	a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+	(6Z,9Z,12Z)-octadeca-6,9,12-trienoyl-[glycerolipid] i.e. gamma-linolenoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases	Mortierella alpina	a stearidonoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O	(6Z,9Z,12Z,15Z)-octadeca-6,9,12,15-tetraenoyl-[glycerolipid] i.e.stearidonoyl-[glycerolipid]	?	431450	
1.14.19.25	a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+	(6Z,9Z,12Z)-octadeca-6,9,12-trienoyl-[glycerolipid] i.e. gamma-linolenoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases	Mortierella alpina 1S-4	a stearidonoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O	(6Z,9Z,12Z,15Z)-octadeca-6,9,12,15-tetraenoyl-[glycerolipid] i.e.stearidonoyl-[glycerolipid]	?	431450	
1.14.19.25	a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+	-	Arabidopsis thaliana	an alpha-linolenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O	-	?	430843	
1.14.19.25	a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+	(9Z,12Z)-octadeca-9,12-dienoyl-[glycerolipid] i.e. linoleoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases	Mortierella alpina	an alpha-linolenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O	(9Z,12Z,15Z)-octadeca-9,12,15-trienoyl-[glycerolipid] i.e. linolenoyl-[glycerolipid]	?	430843	
1.14.19.25	a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+	(9Z,12Z)-octadeca-9,12-dienoyl-[glycerolipid] i.e. linoleoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases	Mortierella alpina 1S-4	an alpha-linolenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O	(9Z,12Z,15Z)-octadeca-9,12,15-trienoyl-[glycerolipid] i.e. linolenoyl-[glycerolipid]	?	430843	
1.14.19.25	an arachidonoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+	(5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoyl-[glycerolipid] i.e. arachidonoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases	Mortierella alpina	a (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O	-	?	431599	
1.14.19.25	an arachidonoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+	(5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoyl-[glycerolipid] i.e. arachidonoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases	Mortierella alpina 1S-4	a (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O	-	?	431599	
1.14.19.25	arachidonic acid + 2 ferrocytochrome b5 + O2 + 2 H+	-	Linum usitatissimum	eicosapentaenoic acid + 2 ferricytochrome b5 + 2 H2O	-	?	433424	
1.14.19.25	arachidonoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+	-	Saprolegnia diclina	eicosapentaenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O	-	?	443063	
1.14.19.25	linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+	-	Brassica napus	alpha-linolenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O	-	?	432834	
1.14.19.25	linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+	-	Brassica oleracea	alpha-linolenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O	-	?	432834	
1.14.19.25	linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+	-	Linum usitatissimum	alpha-linolenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O	-	?	432834	
1.14.19.25	linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+	-	Glycine max	alpha-linolenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O	-	?	432834	
1.14.19.25	additional information	the enzyme shows no DELTA12-desaturase, DELTA6-desaturase or DELTA5-desaturase activity	Mortierella alpina	?	-	?	89	
1.14.19.25	additional information	the enzyme shows no DELTA12-desaturase, DELTA6-desaturase or DELTA5-desaturase activity	Mortierella alpina 1S-4	?	-	?	89	
1.14.19.25	omega-6 linoleic acid + 2 ferrocytochrome b5 + O2 + 2 H+	best substrate	Linum usitatissimum	linolenic acid + 2 ferricytochrome b5 + 2 H2O	-	?	434090