1.14.18.3 1,1,1-trifluoropropane + reduced acceptor + H+ + O2 - Methylococcus capsulatus (2R)-1,1,1-trifluoropropan-2-ol + (2S)-1,1,1-trifluoropropan-2-ol + acceptor + H2O the S stereoisomer is the dominant product ? 395177 1.14.18.3 1,3-butadiene + duroquinol + O2 - Methylosinus trichosporium 1,2-epoxybut-3-ene + duroquinone + H2O 100% 1,2-epoxybut-3-ene is produced, 36% (R)-selectivity, 64% (S)-selectivity ? 415989 1.14.18.3 1-bromopropane + duroquinol + O2 - Methylosinus trichosporium 1-bromo-2-propanol + 1-propanol + 1-bromo-3-propanol + duroquinone + H2O 72% 1-bromo-2-propanol (70% (R)-selectivity, 30% (S)-selectivity), 24% 1-propanol and 4% 1-bromo-3-propanol are produced ? 415999 1.14.18.3 1-bromopropene + duroquinol + O2 - Methylosinus trichosporium 1-bromo-2,3-epoxypropane + allyl-alcohol + 1-propanol + duroquinone + H2O 63% 1-bromo-2,3-epoxypropane, 31% allyl-alcohol and 6% 1-propanol are produced ? 416000 1.14.18.3 1-butene + duroquinol + O2 - Methylosinus trichosporium 1,2-epoxybutane + duroquinone + H2O 100% epoxybutane is produced, 36% (R)-selectivity, 64% (S)-selectivity ? 416001 1.14.18.3 1-butene + reduced acceptor + H+ + O2 - Methylococcus capsulatus 1,2-epoxybutane + acceptor + H2O - ? 395237 1.14.18.3 1-chloropropane + duroquinol + O2 - Methylosinus trichosporium 1-chloro-2-propanol + 1-propanol + 1-chloro-3-propanol + duroquinone + H2O 64% 1-chloro-2-propanol (70% (R)-selectivity, 30% (S)-selectivity), 29% 1-propanol and 7% 1-chloro-3-propanol are produced ? 416003 1.14.18.3 1-chloropropene + duroquinol + O2 - Methylosinus trichosporium 1-chloro-2,3-epoxypropane + allyl-alcohol + 1-propanol + duroquinone + H2O 67% 1-chloro-2,3-epoxypropane, 23% allyl-alcohol and 10% 1-propanol are produced ? 416004 1.14.18.3 2 1-butene + 2 reduced acceptor + 2 H+ + 2 O2 - Methylococcus capsulatus 3-buten-2-ol + 1,2-epoxybutane + 2 acceptor + 2 H2O - ? 395238 1.14.18.3 2 butane + 2 reduced acceptor + 2 H+ + 2 O2 - Methylococcus capsulatus 1-butanol + 2-butanol + 2 acceptor + 2 H2O - ? 396630 1.14.18.3 2 pentane + 2 reduced acceptor + 2 H+ + 2 O2 - Methylococcus capsulatus 1-pentanol + 2-pentanol + 2 acceptor + 2 H2O - ? 398078 1.14.18.3 2 propane + 2 reduced acceptor + 2 H+ + 2 O2 - Methylococcus capsulatus 1-propanol + 2-propanol + 2 acceptor + 2 H2O - ? 398174 1.14.18.3 2-bromopropane + duroquinol + O2 - Methylosinus trichosporium 2-bromo-1-propanol + acetone + duroquinone + H2O 2-bromo-1-propanol shows 26% (R)-selectivity and 74% (S)-selectivity ? 416114 1.14.18.3 2-chloropropane + duroquinol + O2 - Methylosinus trichosporium 2-chloro-1-propanol + ? + duroquinone + H2O 1-chloro-2-propanol shows 26% (R)-selectivity and 74% (S)-selectivity ? 416123 1.14.18.3 3 cis-2-butene + reduced acceptor + H+ + O2 - Methylococcus capsulatus meso-2,3-dimethyloxirane + acceptor + H2O - ? 396703 1.14.18.3 3,3,3-trifluoroprop-1-ene + reduced acceptor + H+ + O2 - Methylococcus capsulatus (2S)-2-(trifluoromethyl)oxirane + (2R)-2-(trifluoromethyl)oxirane + acceptor + H2O the S stereoisomer is the dominant product ? 395667 1.14.18.3 3,3,3-trifluoropropene + reduced acceptor + H+ + O2 - Methylococcus capsulatus 3,3,3-trifluoro-1,2-epoxypropane + acceptor + H2O - ? 395668 1.14.18.3 butane + duroquinol + O2 - Methylosinus trichosporium 2-butanol + butanal + duroquinone + H2O 91% 2-butanal and 9% butanal are produced ? 416736 1.14.18.3 cis-2-butene + duroquinol + O2 - Methylosinus trichosporium cis-2,3-epoxybutane + duroquinone + H2O cis-2,3-epoxybutane is produced ? 416780 1.14.18.3 cis-but-2-ene + reduced acceptor + H+ + O2 - Methylococcus capsulatus cis-2,3-dimethyloxirane + acceptor + H2O - ? 396705 1.14.18.3 ethane + duroquinol + O2 - Methylosinus trichosporium ethanal + duroquinone + H2O 100% ethanal is produced ? 416980 1.14.18.3 ethylene + duroquinol + O2 - Methylosinus trichosporium epoxyethane + duroquinone + H2O 100% epoxyethane produced ? 416991 1.14.18.3 methane + duroquinol + O2 - Methylococcus capsulatus methanol + duroquinone + H2O - ? 389158 1.14.18.3 methane + duroquinol + O2 - Methylomicrobium album methanol + duroquinone + H2O - ? 389158 1.14.18.3 methane + duroquinol + O2 - Methylocystis sp. methanol + duroquinone + H2O - ? 389158 1.14.18.3 methane + duroquinol + O2 - Methylosinus trichosporium methanol + duroquinone + H2O 100% methanol is produced ? 389158 1.14.18.3 methane + duroquinol + O2 - Methylococcus capsulatus Bath methanol + duroquinone + H2O - ? 389158 1.14.18.3 methane + duroquinol + O2 - Methylomicrobium album BG8 methanol + duroquinone + H2O - ? 389158 1.14.18.3 methane + duroquinol + O2 - Methylocystis sp. Rockwell methanol + duroquinone + H2O - ? 389158 1.14.18.3 methane + NADH + O2 - Methylococcus capsulatus methanol + NAD+ + H2O - ? 377156 1.14.18.3 methane + NADH + O2 - Methylococcus capsulatus Bath methanol + NAD+ + H2O - ? 377156 1.14.18.3 methane + quinol + O2 - Methylocystis sp. methanol + quinone + H2O - ? 442474 1.14.18.3 methane + quinol + O2 - Methylococcus capsulatus methanol + quinone + H2O - ? 442474 1.14.18.3 methane + quinol + O2 - Methylocystis sp. M methanol + quinone + H2O - ? 442474 1.14.18.3 methane + quinol + O2 - Methylococcus capsulatus Bath methanol + quinone + H2O - ? 442474 1.14.18.3 methane + quinol + O2 - Methylocystis sp. Rockwell methanol + quinone + H2O - ? 442474 1.14.18.3 methane + reduced acceptor + H* + O2 - Methylococcus capsulatus methanol + acceptor + H2O - ? 448353 1.14.18.3 methane + reduced acceptor + H* + O2 - Methylosinus trichosporium methanol + acceptor + H2O - ? 448353 1.14.18.3 methane + reduced acceptor + H* + O2 - Methylomicrobium album methanol + acceptor + H2O - ? 448353 1.14.18.3 methane + reduced acceptor + H* + O2 - Methylomonas sp. methanol + acceptor + H2O - ? 448353 1.14.18.3 methane + reduced acceptor + H* + O2 - Methylosinus trichosporium OB3b methanol + acceptor + H2O - ? 448353 1.14.18.3 methane + reduced acceptor + H* + O2 - Methylocystis sp. methanol + acceptor + H2O - ? 448353 1.14.18.3 methane + reduced acceptor + H* + O2 - Methylotuvimicrobium japanense methanol + acceptor + H2O - ? 448353 1.14.18.3 methane + reduced acceptor + H* + O2 - uncultured bacterium methanol + acceptor + H2O - ? 448353 1.14.18.3 methane + reduced acceptor + H* + O2 - Methylococcus capsulatus Bath methanol + acceptor + H2O - ? 448353 1.14.18.3 methane + reduced acceptor + H* + O2 - Methylocystis sp. Sc2 methanol + acceptor + H2O - ? 448353 1.14.18.3 methane + reduced acceptor + H* + O2 - Methylococcus capsulatus M methanol + acceptor + H2O - ? 448353 1.14.18.3 methane + reduced acceptor + H* + O2 - Methylotuvimicrobium japanense NI methanol + acceptor + H2O - ? 448353 1.14.18.3 methane + trans-dichloroethylene + vinyl chloride + trichloroethylene + O2 - Methylosinus trichosporium ? - ? 417257 1.14.18.3 Mn2+ + H2O2 - Methylococcus capsulatus ? - ? 389178 1.14.18.3 Mn2+ + H2O2 - Methylosinus trichosporium ? - ? 389178 1.14.18.3 Mn2+ + H2O2 - Methylomonas sp. ? - ? 389178 1.14.18.3 Mn2+ + H2O2 - Methylocystis sp. ? - ? 389178 1.14.18.3 Mn2+ + H2O2 - Methylococcus capsulatus Bath ? - ? 389178 1.14.18.3 Mn2+ + H2O2 - Methylococcus capsulatus M ? - ? 389178 1.14.18.3 n-butane + reduced acceptor + O2 - Methylosinus trichosporium 2-butanol + acceptor + H2O - ? 428806 1.14.18.3 n-butane + reduced acceptor + O2 - Methylocystis sp. 2-butanol + acceptor + H2O - ? 428806 1.14.18.3 n-butane + reduced acceptor + O2 - Methylococcus capsulatus 2-butanol + acceptor + H2O - ? 428806 1.14.18.3 n-pentane + reduced acceptor + O2 - Methylosinus trichosporium 2-pentanol + acceptor + H2O - ? 428810 1.14.18.3 n-pentane + reduced acceptor + O2 - Methylocystis sp. 2-pentanol + acceptor + H2O - ? 428810 1.14.18.3 n-pentane + reduced acceptor + O2 - Methylococcus capsulatus 2-pentanol + acceptor + H2O - ? 428810 1.14.18.3 pentane + duroquinol + O2 - Methylosinus trichosporium 2-pentanol + pentanal + duroquinone + H2O 31% 2-pentanal and 69% pentanal are produced ? 417462 1.14.18.3 propane + duroquinol + O2 - Methylosinus trichosporium 2-propanol + propanal + duroquinone + H2O 84% 2-propanal and 16% propanal are produced ? 417526 1.14.18.3 propene + duroquinol + O2 - Methylosinus trichosporium 1,2-epoxypropane + duroquinone + H2O 57% (R)-selectivity, 43% (S)-selectivity ? 417528 1.14.18.3 propene + reduced acceptor + H+ + O2 - Methylococcus capsulatus 1,2-epoxypropane + acceptor + H2O - ? 398178 1.14.18.3 propene + reduced acceptor + H+ + O2 - Methylococcus capsulatus Bath 1,2-epoxypropane + acceptor + H2O - ? 398178 1.14.18.3 propylene + 2,3-dimethylquinol + O2 - Methylococcus capsulatus propylene oxide + 2,3-dimethylquinone + H2O - ? 417536 1.14.18.3 propylene + 2,3-dimethylquinol + O2 - Methylococcus capsulatus Bath propylene oxide + 2,3-dimethylquinone + H2O - ? 417536 1.14.18.3 propylene + coenzyme Q0 + O2 - Methylococcus capsulatus propylene oxide + reduced coenzyme Q0 + H2O - ? 417537 1.14.18.3 propylene + coenzyme Q0 + O2 - Methylococcus capsulatus Bath propylene oxide + reduced coenzyme Q0 + H2O - ? 417537 1.14.18.3 propylene + decylubiquinol + O2 - Methylococcus capsulatus propylene oxide + decylubiquinone + H2O - ? 417539 1.14.18.3 propylene + duroquinol + O2 - Methylococcus capsulatus propylene oxide + reduced duroquinol + H2O - ? 377552 1.14.18.3 propylene + duroquinol + O2 - Methylococcus capsulatus Bath propylene oxide + reduced duroquinol + H2O - ? 377552 1.14.18.3 propylene + duroquinol + O2 - Methylococcus capsulatus propylene epoxide + duroquinone + H2O - ? 389606 1.14.18.3 propylene + duroquinol + O2 - Methylococcus capsulatus Bath propylene epoxide + duroquinone + H2O - ? 389606 1.14.18.3 propylene + duroquinol + O2 - Methylosinus trichosporium epoxypropane + allyl-alcohol + 1-propanol + duroquinone + H2O 95% epoxypropane, 4.6% allyl-alcohol and 0.4% butanal are produced ? 417540 1.14.18.3 propylene + duroquinol + O2 - Methylococcus capsulatus propylene oxide + duroquinone + H2O - ? 417541 1.14.18.3 propylene + duroquinol + O2 - Methylococcus capsulatus Bath propylene oxide + duroquinone + H2O - ? 417541 1.14.18.3 propylene + duroquinol + O2 - Methylococcus capsulatus ATCC 33009 propylene oxide + duroquinone + H2O - ? 417541 1.14.18.3 propylene + menaquinol + O2 - Methylococcus capsulatus propylene oxide + menaquinone + H2O - ? 417542 1.14.18.3 propylene + trimethylquinol + O2 - Methylococcus capsulatus propylene oxide + trimethylquinone + H2O - ? 417544 1.14.18.3 trans-2-butene + duroquinol + O2 - Methylosinus trichosporium trans-2,3-epoxybutane + trans-2-butane-1-al + duroquinone + H2O 41% trans-2,3-epoxybutane and 59% trans-2-butane-1-al are produced ? 417709 1.14.18.3 trans-2-butene + reduced acceptor + H+ + O2 - Methylococcus capsulatus 2,3-dimethyloxirane + acceptor + H2O - ? 398436 1.14.18.3 trans-but-2-ene + reduced acceptor + H+ + O2 - Methylococcus capsulatus (2R,3R)-trans-2,3-dimethyloxirane + (2S,3S)-trans-2,3-dimethyloxirane + acceptor + H2O the S,S stereoisomer is the dominant product ? 398440 1.14.18.3 additional information activity assays on membrane-bound pMMO routinely utilize NADH, succinate, or duroquinol as reductant, while only duroquinol and to a lesser extent, other quinols, are effective for solubilized and purified samples Methylococcus capsulatus ? - ? 89 1.14.18.3 additional information activity assays on membrane-bound pMMO routinely utilize NADH, succinate, or duroquinol as reductant, while only duroquinol and to a lesser extent, other quinols, are effective for solubilized and purified samples Methylococcus capsulatus Bath ? - ? 89 1.14.18.3 additional information decyl-plastoquinol, reduced coenzyme Q1, and trimethylquinol can drive pMMO, though its activity is lower than that with duroquinol. Succinate-driven pMMO activity in the membrane fractions is also observed Methylosinus trichosporium ? - ? 89 1.14.18.3 additional information duroquinol, an electron donor for pMMO may induce the formation of H2O2 by pMMO under aerobic conditions Methylosinus trichosporium OB3b ? - ? 89 1.14.18.3 propene + reduced acceptor + H+ + O2 enzyme form sMMO Methylococcus capsulatus 1,2-epoxypropane + acceptor + H2O - ? 398178 1.14.18.3 propene + reduced acceptor + H+ + O2 enzyme form sMMO Methylococcus capsulatus Bath 1,2-epoxypropane + acceptor + H2O - ? 398178 1.14.18.3 methane + quinol + O2 enzyme pMMO activity is dependent on oxygen concentrations Methylococcus capsulatus methanol + quinone + H2O - ? 442474 1.14.18.3 methane + quinol + O2 enzyme pMMO activity is dependent on oxygen concentrations Methylococcus capsulatus Bath methanol + quinone + H2O - ? 442474 1.14.18.3 propylene + decyl-plastoquinol + O2 higher activity compared to duroquinol Methylococcus capsulatus propylene oxide + decyl-plastoquinone + H2O - ? 417538 1.14.18.3 propylene + decyl-plastoquinol + O2 higher activity compared to duroquinol Methylococcus capsulatus Bath propylene oxide + decyl-plastoquinone + H2O - ? 417538 1.14.18.3 methane + reduced acceptor + H* + O2 in the presence of pMMO substrate methane, the H2O2 formation is diminished, which is likely to be caused by the consumption of electrons by methane oxidation Methylosinus trichosporium OB3b methanol + acceptor + H2O - ? 448353 1.14.18.3 methane + succinate + O2 membrane-bound enzyme only Methylococcus capsulatus methanol + fumarate + H2O - ? 443727 1.14.18.3 methane + succinate + O2 membrane-bound enzyme only Methylococcus capsulatus Bath methanol + fumarate + H2O - ? 443727 1.14.18.3 additional information membrane-bound pMMO can efficiently oxidize straight-chain hydrocarbons from C1 to C5 with high regiospecificity and unusual stereoselectivity. Acetylene is a suicide substrate/inhibitor, the enzyme oxidizes acetylene to the ketene (C2H2O) intermediate, which then forms an acetylation adduct with the transmembrane PmoC subunit, there is a thermodynamic driving force for a ketene formed at the catalytic site to find its way to the water-exposed domain of subunit PmoB for acetylation, residue K196 of subunit PmoC that is acetylated, overview Methylococcus capsulatus ? - ? 89 1.14.18.3 additional information membrane-bound pMMO can efficiently oxidize straight-chain hydrocarbons from C1 to C5 with high regiospecificity and unusual stereoselectivity. Acetylene is a suicide substrate/inhibitor, the enzyme oxidizes acetylene to the ketene (C2H2O) intermediate, which then forms an acetylation adduct with the transmembrane PmoC subunit, there is a thermodynamic driving force for a ketene formed at the catalytic site to find its way to the water-exposed domain of subunit PmoB for acetylation, residue K196 of subunit PmoC that is acetylated, overview Methylococcus capsulatus Bath ? - ? 89 1.14.18.3 methane + quinol + O2 methane activation occurs at the Cu centers of particulate methane monooxygenase Methylosinus trichosporium methanol + quinone + H2O - ? 442474 1.14.18.3 methane + quinol + O2 methane activation occurs at the Cu centers of particulate methane monooxygenase Methylocystis sp. methanol + quinone + H2O - ? 442474 1.14.18.3 methane + quinol + O2 methane activation occurs at the Cu centers of particulate methane monooxygenase Methylocystis sp. Rockwell methanol + quinone + H2O - ? 442474 1.14.18.3 additional information methane oxidation activity of apo membrane-bound Methylococcus capsulatus (Bath) pMMO after metal loading using two copper reconstitution methods, overview Methylococcus capsulatus ? - ? 89 1.14.18.3 additional information methane oxidation activity of apo membrane-bound Methylococcus capsulatus (Bath) pMMO after metal loading using two copper reconstitution methods, overview Methylococcus capsulatus Bath ? - ? 89 1.14.18.3 methane + quinol + O2 Methylococcus capsulatus (Bath) is a methanotroph that possesses both a membrane-embedded (pMMO) and a soluble methane monooxygenase (sMMO). Major changes takes place in the respiratory chain between pMMO- and sMMO-producing cells. Quinones are predominantly used as the electron donors for methane oxidation by pMMO. During production of particulate methane monooxygenase, the majority of quinones are directed to methane oxidation Methylococcus capsulatus methanol + quinone + H2O - ? 442474 1.14.18.3 methane + quinol + O2 Methylococcus capsulatus (Bath) is a methanotroph that possesses both a membrane-embedded (pMMO) and a soluble methane monooxygenase (sMMO). Major changes takes place in the respiratory chain between pMMO- and sMMO-producing cells. Quinones are predominantly used as the electron donors for methane oxidation by pMMO. During production of particulate methane monooxygenase, the majority of quinones are directed to methane oxidation Methylococcus capsulatus Bath methanol + quinone + H2O - ? 442474 1.14.18.3 methane + quinol + O2 Methylococcus capsulatus (Bath) is a methanotroph that possesses both a membrane-embedded (pMMO) and a soluble methane monooxygenase (sMMO). Major changes takes place in the respiratory chain between pMMO- and sMMO-producing cells. Quinones are predominantly used as the electron donors for methane oxidation by pMMO. During production of particulate methane monooxygenase, the majority of quinones are directed to methane oxidation Methylococcus capsulatus Bath. methanol + quinone + H2O - ? 442474 1.14.18.3 additional information pMMO cannot oxidize naphthalene Methylococcus capsulatus ? - ? 89 1.14.18.3 additional information pMMO cannot oxidize naphthalene Methylosinus trichosporium ? - ? 89 1.14.18.3 additional information pMMO cannot oxidize naphthalene Methylomonas sp. ? - ? 89 1.14.18.3 additional information pMMO has narrower substrate specificity but higher activity with smaller hydrocarbons like methane, ethane, and propene compared to sMMO Methylococcus capsulatus ? - ? 89 1.14.18.3 additional information pMMO has narrower substrate specificity but higher activity with smaller hydrocarbons like methane, ethane, and propene compared to sMMO Methylosinus trichosporium ? - ? 89 1.14.18.3 additional information pMMO has narrower substrate specificity but higher activity with smaller hydrocarbons like methane, ethane, and propene compared to sMMO Methylomonas sp. ? - ? 89 1.14.18.3 methane + reduced acceptor + H* + O2 pmoA cluster JR3 may be the most important methane oxidizer for arid soils Soil bacterium methanol + acceptor + H2O - ? 448353 1.14.18.3 additional information quinols are effective reductants for the detergent-solubilized enzyme, whereas NADH is ineffective Methylococcus capsulatus ? - ? 89 1.14.18.3 additional information quinols are effective reductants for the detergent-solubilized enzyme, whereas NADH is ineffective Methylococcus capsulatus Bath ? - ? 89 1.14.18.3 additional information unlike the sMMO, the pMMO enzyme has relatively narrow substrate specificity, oxidising alkanes and alkenes of up to five carbons but not aromatic compounds Methylococcus capsulatus ? - ? 89 1.14.18.3 additional information unlike the sMMO, the pMMO enzyme has relatively narrow substrate specificity, oxidising alkanes and alkenes of up to five carbons but not aromatic compounds Methylomicrobium album ? - ? 89 1.14.18.3 additional information unlike the sMMO, the pMMO enzyme has relatively narrow substrate specificity, oxidising alkanes and alkenes of up to five carbons but not aromatic compounds Methylocystis sp. ? - ? 89 1.14.18.3 additional information unlike the sMMO, the pMMO enzyme has relatively narrow substrate specificity, oxidising alkanes and alkenes of up to five carbons but not aromatic compounds Methylococcus capsulatus Bath ? - ? 89 1.14.18.3 additional information unlike the sMMO, the pMMO enzyme has relatively narrow substrate specificity, oxidising alkanes and alkenes of up to five carbons but not aromatic compounds Methylomicrobium album BG8 ? - ? 89