1.14.14.29 17beta-estradiol + NADPH + H+ + O2 - Rattus norvegicus 1,3,5(10)-estratrien-3,7-alpha,17beta-triol + NADP+ + H2O - ? 375583 1.14.14.29 22-hydroxycholesterol + NADPH + H+ + O2 - Mus musculus 7alpha,22-dihydroxycholesterol + NADP+ + H2O - ? 375719 1.14.14.29 24,25-epoxycholesterol + NADPH + H+ + O2 - Mus musculus 7alpha,hydroxy-24,25-epoxycholesterol + NADP+ + H2O - ? 375725 1.14.14.29 24-hydroxycholesterol + NADPH + H+ + O2 - Mus musculus 7alpha,24-dihydroxycholesterol + NADP+ + H2O - ? 375728 1.14.14.29 25-hydroxycholesterol + NADPH + H+ + O2 - Homo sapiens ? + NADP+ + H2O - ? 427293 1.14.14.29 27-hydroxycholesterol + NADPH + H+ + O2 - Homo sapiens 3beta,7alpha-dihydroxy-5-cholestenoic acid + NADP+ + H2O - ? 427296 1.14.14.29 4-androstene-3,17-dione + NADPH + H+ + O2 - Rattus norvegicus 7alpha-hydroxy-4-androstene-3,17-dione + NADP+ + H2O - ? 375865 1.14.14.29 5alpha-androstane-3alpha,17beta-diol + NADPH + O2 - Homo sapiens 5alpha-androstane-3alpha,7alpha,17beta-triol + NADP+ + H2O major product, plus some hydroxylation at position 6 ? 408213 1.14.14.29 5alpha-androstane-3alpha,17beta-diol + NADPH + O2 - Sus scrofa 5alpha-androstane-3alpha,7alpha,17beta-triol + NADP+ + H2O major product, plus some hydroxylation at position 6 ? 408213 1.14.14.29 5alpha-androstane-3alpha,17beta-diol + NADPH + O2 - Sus scrofa ? + NADP+ + H2O - ? 408214 1.14.14.29 5alpha-androstane-3alpha,17beta-diol + NADPH + O2 - Homo sapiens ? + NADP+ + H2O - ? 408214 1.14.14.29 5alpha-androstane-3alpha,17beta-diol + NADPH + O2 - Mus musculus ? + NADP+ + H2O - ? 408214 1.14.14.29 5alpha-androstane-3beta,17beta-diol + NADPH + H+ + O2 - Rattus norvegicus 5alpha,androstane-3beta,7alpha,17beta-triol + NADP+ + H2O - ? 376011 1.14.14.29 5alpha-androstane-3beta,17beta-diol + NADPH + H+ + O2 - Homo sapiens ? - ? 396040 1.14.14.29 5alpha-androstane-3beta,17beta-diol + NADPH + H+ + O2 - Sus scrofa ? - ? 396040 1.14.14.29 cholest-5-ene-3beta,25-diol + NADPH + H+ + O2 - Mus musculus cholest-5-ene-3beta,7alpha,25-triol + NADP+ + H2O - ? 375087 1.14.14.29 cholest-5-ene-3beta,25-diol + NADPH + H+ + O2 - Homo sapiens cholest-5-ene-3beta,7alpha,25-triol + NADP+ + H2O - ? 375087 1.14.14.29 cholest-5-ene-3beta,25-diol + NADPH + H+ + O2 - Rattus norvegicus cholest-5-ene-3beta,7alpha,25-triol + NADP+ + H2O - ? 375087 1.14.14.29 cholest-5-ene-3beta,25-diol + NADPH + H+ + O2 - Sus scrofa cholest-5-ene-3beta,7alpha,25-triol + NADP+ + H2O - ? 375087 1.14.14.29 cholest-5-ene-3beta,25-diol + NADPH + H+ + O2 CYP7B1 oxysterol 7alpha-hydroxylase pathway synthesizes 25% to 30% of all bile acids Mus musculus cholest-5-ene-3beta,7alpha,25-triol + NADP+ + H2O - ? 375087 1.14.14.29 cholest-5-ene-3beta,25-diol + NADPH + H+ + O2 dietary cholesterol or colestipol do not affect oxysterol 7alpha-hydroxylase enzyme activity, mRNA, or protein levels Mus musculus cholest-5-ene-3beta,7alpha,25-triol + NADP+ + H2O - ? 375087 1.14.14.29 cholest-5-ene-3beta,25-diol + NADPH + H+ + O2 loss of the enzyme in liver is compensated for by increases in the synthesis of bile acids by other pathways Mus musculus cholest-5-ene-3beta,7alpha,25-triol + NADP+ + H2O - ? 375087 1.14.14.29 cholest-5-ene-3beta,25-diol + NADPH + H+ + O2 substrates in descending order of activity: cholest-5-ene-3beta,25-diol, cholest-5-ene-3beta,27-diol, 24,25-epoxycholesterol, 22-hydroxycholesterol, 24-hydroxycholesterol Mus musculus cholest-5-ene-3beta,7alpha,25-triol + NADP+ + H2O - ? 375087 1.14.14.29 cholest-5-ene-3beta,27-diol + NADPH + H+ + O2 - Mus musculus cholest-5-ene-3beta,7alpha,27-triol + NADP+ + H2O - ? 375088 1.14.14.29 cholest-5-ene-3beta,27-diol + NADPH + H+ + O2 - Homo sapiens cholest-5-ene-3beta,7alpha,27-triol + NADP+ + H2O - ? 375088 1.14.14.29 cholest-5-ene-3beta,27-diol + NADPH + H+ + O2 CYP7B1 oxysterol 7alpha-hydroxylase pathway synthesizes 25% to 30% of all bile acids Mus musculus cholest-5-ene-3beta,7alpha,27-triol + NADP+ + H2O - ? 375088 1.14.14.29 cholest-5-ene-3beta,27-diol + NADPH + H+ + O2 loss of the enzyme in liver is compensated for by increases in the synthesis of bile acids by other pathways Mus musculus cholest-5-ene-3beta,7alpha,27-triol + NADP+ + H2O - ? 375088 1.14.14.29 dehydroepiandrosterone + NADPH + H+ + O2 - Rattus norvegicus 3beta,7alpha-dihydroxy-5-androsten-17-one + NADP+ + H2O - ? 375106 1.14.14.29 dehydroepiandrosterone + NADPH + H+ + o2 - Homo sapiens 7alpha-hydroxy-dehydroepiandrosterone + NADP+ + H2O - ? 396974 1.14.14.29 dehydroepiandrosterone + NADPH + H+ + o2 - Sus scrofa 7alpha-hydroxy-dehydroepiandrosterone + NADP+ + H2O - ? 396974 1.14.14.29 dehydroepiandrosterone + NADPH + H+ + o2 - Mus musculus 7alpha-hydroxy-dehydroepiandrosterone + NADP+ + H2O - ? 396974 1.14.14.29 dehydroepiandrosterone + NADPH + H+ + O2 - Homo sapiens ? + NADP+ + H2O - ? 409268 1.14.14.29 dehydroepiandrosterone + NADPH + H+ + O2 - Sus scrofa ? + NADP+ + H2O - ? 409268 1.14.14.29 additional information both phorbol myristate acetate and cAMP decreases CYP7B1 activity by 60% and 34% respectively, in a time-dependent fashion. Changes in CYP7B1 messenger RNA levels correlate with changes in specific activities. CYP7B1 specific activity is highly regulated but does not seem to be rate limiting for bile acid synthesis Rattus norvegicus ? - ? 89 1.14.14.29 additional information regulation of CYP7B1 transcription by Sp1 may play a pivotal role in regulating oxysterol levels, which regulate cholesterol metabolism Homo sapiens ? - ? 89 1.14.14.29 additional information the enzyme is regulated by bile acids and cholesterol Rattus norvegicus ? - ? 89 1.14.14.29 additional information retinoid-related orphan receptor alpha positively and directly regulates the expression of Cyp7b1 Mus musculus ? - ? 89