1.14.13.130 additional information no enzymatic activity is detected with the analogous aromatic heterocyclic compounds furan-2-carboxylate and furan-3-carboxylate, and thiophene-2-carboxylate and thiophene-3-carboxylate (0.25 mM). Pyrrole, proline, indole, and indole-2-carboxylate (each 0.25 mM) do not serve as a substrate or effector of NADH oxidation for pyrrole-2-carboxylate monooxygenase. Chlorinated phenols and 4-hydroxyphenylacetate, which are substrates for the structurally related two-component flavin aromatic monooxygenases isolated from different sources, do not affect NADH oxidation or oxygen consumption catalyzed by pyrrole-2-carboxylate monooxygenase Rhodococcus sp. ? 89 1.14.13.130 pyrrole-2-carboxylate + NADH + H+ + O2 no activity with NADPH Rhodococcus sp. 5-hydroxypyrrole-2-carboxylate + NAD+ + H2O 410974 1.14.13.130 pyrrole-2-carboxylate + NADH + H+ + O2 the enzyme initiates the degradation of pyrrole-2-carboxylate. Growth on pyrrole-2-carboxylate as the sole source of carbon, nitrogen and energy Arthrobacter sp. 5-hydroxypyrrole-2-carboxylate + NAD+ + H2O 410974 1.14.13.130 pyrrole-2-carboxylate + NADH + H+ + O2 the enzyme initiates the degradation of pyrrole-2-carboxylate. Growth on pyrrole-2-carboxylate as the sole source of carbon, nitrogen and energy Rhodococcus sp. 5-hydroxypyrrole-2-carboxylate + NAD+ + H2O 410974 1.14.13.130 pyrrole-2-carboxylate + NADH + H+ + O2 no activity with NADPH. Narrow substrate specificity. Besides pyrrole-2-carboxylate, only pyrrole, pyrrole-2-aldehyde, and indole-2-carboxylate stimulate the oxygen consumption at a very low rate Arthrobacter sp. 5-hydroxypyrrole-2-carboxylate + NAD+ + H2O 410974 1.14.13.130 pyrrole-2-carboxylate + NADH + H+ + O2 the enzyme initiates the degradation of pyrrole-2-carboxylate. Growth on pyrrole-2-carboxylate as the sole source of carbon, nitrogen and energy Arthrobacter sp. Py1 5-hydroxypyrrole-2-carboxylate + NAD+ + H2O 410974 1.14.13.130 pyrrole-2-carboxylate + NADH + H+ + O2 no activity with NADPH. Narrow substrate specificity. Besides pyrrole-2-carboxylate, only pyrrole, pyrrole-2-aldehyde, and indole-2-carboxylate stimulate the oxygen consumption at a very low rate Arthrobacter sp. Py1 5-hydroxypyrrole-2-carboxylate + NAD+ + H2O 410974