1.14.11.8	additional information	enzyme is localized to the mitochondrial matrix, product formation is limited by 6-N-trimethyllysine transport across the mitochondrial inner membrane	Rattus norvegicus	?	-	?	89	
1.14.11.8	additional information	determination of the (2S,3S)-3-hydroxy-N6,N6,N6-trimethyl-L-lysine and N6,N6,N6-trimethyl-L-lysine is performed by ultraperformance liquid chromatography–tandem mass spectrometry (UPLC/MS/MS) in positive ion electrospray mode	Homo sapiens	?	-	?	89	
1.14.11.8	additional information	NMR spectrosopic structure analysis of substrates and products	Homo sapiens	?	-	?	89	
1.14.11.8	additional information	NMR spectrosopic structure analysis of substrates and products, substrate specificity, overview. No or poor activity with dimethyllysine, methyllysine, lysine, D-trimethyllysine, 5-trimethylamino-1-aminopentane, trimethyllysine, 6-trimethylaminohexanoic acid, hexamethyllysine, N-acetyltrimethyllysine, trimethyldiaminobutyric acid, triethyllysine, symmetric and asymmetric dimethylarginines, mildronate, and gamma-butyrobetaine, structure-activity relationship study	Homo sapiens	?	-	?	89	
1.14.11.8	N5,N5,N5-trimethyl-L-ornithine + 2-oxoglutarate + O2	-	Homo sapiens	(3S)-3-hydroxy-N5,N5,N5-trimethyl-L-ornithine + succinate + CO2	-	?	443777	
1.14.11.8	N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2	-	Mus musculus	3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2	-	?	2718	
1.14.11.8	N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2	-	Homo sapiens	3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2	-	?	2718	
1.14.11.8	N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2	-	Rattus norvegicus	3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2	-	?	2718	
1.14.11.8	N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2	-	Sus scrofa	3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2	-	?	2718	
1.14.11.8	N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2	-	Neurospora crassa	3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2	-	?	2718	
1.14.11.8	N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2	N6,N6, N6-trimethyl-L-lysine is epsilon-N-trimethyl-L-lysine	Rattus norvegicus	3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2	-	?	2718	
1.14.11.8	N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2	N6,N6, N6-trimethyl-L-lysine is epsilon-N-trimethyl-L-lysine	Bos taurus	3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2	-	?	2718	
1.14.11.8	N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2	N6,N6, N6-trimethyl-L-lysine is epsilon-N-trimethyl-L-lysine	Neurospora crassa	3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2	-	?	2718	
1.14.11.8	N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2	carnitine biosynthesis	Rattus norvegicus	3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2	-	?	2718	
1.14.11.8	N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2	first enzyme of the L-carnitine biosynthesis	Rattus norvegicus	3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2	-	?	2718	
1.14.11.8	N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2	-	Homo sapiens	(3S)-3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2	-	?	442485	
1.14.11.8	N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2	the enzymatic hydroxylation occurs at the C-3 site of (2S)-Nepsilon-trimethyllysine substrate. Comparative NMR spectroscopic studies (with two enantiopure synthetic standards that possess 3R and 3S stereochemistry) on the enzymatically produced (2S)-3-hydroxy-Nepsilon-trimethyllysine reveal that TMLH exclusively catalyzes the formation of (3S)-3-hydroxy-Nepsilon-trimethyl-L-lysine, not forming any (3R)-3-hydroxy-Nepsilon-trimethyl-L-lysine diastereoisomer	Homo sapiens	(3S)-3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2	-	?	442485	
1.14.11.8	N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2	recognition sites that contribute to the enzymatic activity of the enzyme (TMLH): the Fe(II)-binding H242-D244-H389 residues, R391-R398 involved in 2-oxoglutarate-binding, and several residues (D231, N334, and the aromatic cage comprised of W221, Y217 and Y234) associated with binding of (2S)-Nepsilon-trimethyllysine	Homo sapiens	(3S)-3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2	-	?	442485	
1.14.11.8	N6,N6-dimethyl-N6-ethyl-L-lysine + 2-oxoglutarate + O2	-	Homo sapiens	(3S)-3-hydroxy-N6,N6-dimethyl-N6-ethyl-L-lysine + succinate + CO2	-	?	443778	
1.14.11.8	N6,N6-dimethyl-N6-isopropyl-L-lysine + 2-oxoglutarate + O2	-	Homo sapiens	(3S)-3-hydroxy-N6,N6-dimethyl-N6-isopropyl-L-lysine + succinate + CO2	-	?	443779	
1.14.11.8	N6,N6-dimethyl-N6-propyl-L-lysine + 2-oxoglutarate + O2	-	Homo sapiens	(3S)-3-hydroxy-N6,N6-dimethyl-N6-propyl-L-lysine + succinate + CO2	-	?	443780	
1.14.11.8	N6-fluoromethyl-N6,N6-dimethyl-L-lysine + 2-oxoglutarate + O2	Nepsilon-trimethyllysine analogue that contains the fluoromethyl group can be used as a 1H and 19F NMR probe for studies on TMLH catalysis	Homo sapiens	(3S)-3-hydroxy-N6-fluoromethyl-N6,N6-dimethyl-L-lysine + succinate + CO2	-	?	443781	
1.14.11.8	N7,N7,N7-trimethyl-L-alpha-homolysine + 2-oxoglutarate + O2	-	Homo sapiens	(3S)-3-hydroxy-N7,N7,N7-trimethyl-L-alpha-homolysine + succinate + CO2	-	?	443782