1.1.1.67 arabitol + NAD(P)+ - Pseudomonas fluorescens ? + NAD(P)H - ? 404346 1.1.1.67 D-arabinitol + NAD+ - Cereibacter sphaeroides D-ribulose + NADH + H+ - ? 4443 1.1.1.67 D-arabinitol + NAD+ - Bradyrhizobium japonicum D-ribulose + NADH + H+ - ? 4443 1.1.1.67 D-arabinitol + NAD+ - Absidia glauca D-ribulose + NADH + H+ - ? 4443 1.1.1.67 D-arabinitol + NAD+ - Aspergillus fumigatus D-xylulose + NADH + H+ - r 186987 1.1.1.67 D-arabitol + NAD+ - Pseudomonas fluorescens ? - ? 165122 1.1.1.67 D-arabitol + NAD+ - Cereibacter sphaeroides ? + NADH - ? 188545 1.1.1.67 D-arabitol + NAD+ - Bradyrhizobium japonicum ? + NADH - ? 188545 1.1.1.67 D-fructose + NAD(P)+ - Pseudomonas fluorescens ? + NAD(P)H - ? 372840 1.1.1.67 D-fructose + NADH + H+ - Sinorhizobium meliloti D-mannitol + NAD+ - r 372842 1.1.1.67 D-fructose + NADH + H+ - Pseudomonas fluorescens D-mannitol + NAD+ - r 372842 1.1.1.67 D-fructose + NADH + H+ - Levilactobacillus brevis D-mannitol + NAD+ - r 372842 1.1.1.67 D-fructose + NADH + H+ - Thermotoga neapolitana D-mannitol + NAD+ - ? 372842 1.1.1.67 D-fructose + NADH + H+ - Thermotoga neapolitana D-mannitol + NAD+ - r 372842 1.1.1.67 D-fructose + NADH + H+ - Gluconobacter oxydans D-mannitol + NAD+ - ? 372842 1.1.1.67 D-fructose + NADH + H+ - Leuconostoc pseudomesenteroides D-mannitol + NAD+ - ? 372842 1.1.1.67 D-fructose + NADH + H+ - Leuconostoc pseudomesenteroides D-mannitol + NAD+ - r 372842 1.1.1.67 D-fructose + NADH + H+ - Thermotoga maritima D-mannitol + NAD+ - r 372842 1.1.1.67 D-fructose + NADH + H+ - Aspergillus fumigatus D-mannitol + NAD+ - r 372842 1.1.1.67 D-fructose + NADH + H+ - Pseudomonas fluorescens D-mannitol + NAD+ - ? 372842 1.1.1.67 D-fructose + NADH + H+ - Saccharina japonica D-mannitol + NAD+ - ? 372842 1.1.1.67 D-fructose + NADH + H+ Asn300 has an auxiliary role in stabilization of the transition state of hydride transfer and His303 contributes to substrate positioning, role of Lys295 in general base enzymic catalysis Pseudomonas fluorescens D-mannitol + NAD+ - ? 372842 1.1.1.67 D-fructose + NADH + H+ -mannitol production Leuconostoc pseudomesenteroides D-mannitol + NAD+ - r 372842 1.1.1.67 D-fructose + NADH + H+ the Thermotoga maritima MtDH pathway produces D-mannitol from glucose in two steps: first the xylose isomerase from Thermotoga neapolitana converts glucose to fructose, then MtDH converts D-fructose to D-mannitol Thermotoga maritima D-mannitol + NAD+ - r 372842 1.1.1.67 D-fructose + NADH + H+ - Leuconostoc pseudomesenteroides CTCC G123 D-mannitol + NAD+ - ? 372842 1.1.1.67 D-fructose + NADH + H+ - Sinorhizobium meliloti KCTC 2353 D-mannitol + NAD+ - r 372842 1.1.1.67 D-fructose + NADH + H+ - Gluconobacter oxydans LMG 1489 D-mannitol + NAD+ - ? 372842 1.1.1.67 D-fructose + NADH + H+ - Thermotoga maritima DSM 3109 D-mannitol + NAD+ - r 372842 1.1.1.67 D-fructose + NADH + H+ the Thermotoga maritima MtDH pathway produces D-mannitol from glucose in two steps: first the xylose isomerase from Thermotoga neapolitana converts glucose to fructose, then MtDH converts D-fructose to D-mannitol Thermotoga maritima DSM 3109 D-mannitol + NAD+ - r 372842 1.1.1.67 D-fructose + NADH + H+ - Leuconostoc pseudomesenteroides ATCC 12291 D-mannitol + NAD+ - r 372842 1.1.1.67 D-fructose + NADH + H+ -mannitol production Leuconostoc pseudomesenteroides ATCC 12291 D-mannitol + NAD+ - r 372842 1.1.1.67 D-fructose + NADH + H+ - Thermotoga maritima NBRC 100826 D-mannitol + NAD+ - r 372842 1.1.1.67 D-fructose + NADH + H+ the Thermotoga maritima MtDH pathway produces D-mannitol from glucose in two steps: first the xylose isomerase from Thermotoga neapolitana converts glucose to fructose, then MtDH converts D-fructose to D-mannitol Thermotoga maritima NBRC 100826 D-mannitol + NAD+ - r 372842 1.1.1.67 D-fructose + NADH + H+ - Thermotoga maritima ATCC 43589 D-mannitol + NAD+ - r 372842 1.1.1.67 D-fructose + NADH + H+ the Thermotoga maritima MtDH pathway produces D-mannitol from glucose in two steps: first the xylose isomerase from Thermotoga neapolitana converts glucose to fructose, then MtDH converts D-fructose to D-mannitol Thermotoga maritima ATCC 43589 D-mannitol + NAD+ - r 372842 1.1.1.67 D-fructose + NADH + H+ - Thermotoga neapolitana DSM 4359 D-mannitol + NAD+ - ? 372842 1.1.1.67 D-fructose + NADH + H+ - Thermotoga maritima JCM 10099 D-mannitol + NAD+ - r 372842 1.1.1.67 D-fructose + NADH + H+ the Thermotoga maritima MtDH pathway produces D-mannitol from glucose in two steps: first the xylose isomerase from Thermotoga neapolitana converts glucose to fructose, then MtDH converts D-fructose to D-mannitol Thermotoga maritima JCM 10099 D-mannitol + NAD+ - r 372842 1.1.1.67 D-fructose + NADH + H+ - Pseudomonas fluorescens DSM 50106 D-mannitol + NAD+ - r 372842 1.1.1.67 D-fructose + NADPH + H+ low activity Thermotoga maritima D-mannitol + NADP+ - r 384520 1.1.1.67 D-fructose + NADPH + H+ also active on fructose with NADPH Thermotoga maritima D-mannitol + NADP+ - ? 384520 1.1.1.67 D-fructose + NADPH + H+ low activity Thermotoga maritima DSM 3109 D-mannitol + NADP+ - r 384520 1.1.1.67 D-fructose + NADPH + H+ low activity Thermotoga maritima NBRC 100826 D-mannitol + NADP+ - r 384520 1.1.1.67 D-fructose + NADPH + H+ low activity Thermotoga maritima ATCC 43589 D-mannitol + NADP+ - r 384520 1.1.1.67 D-fructose + NADPH + H+ also active on fructose with NADPH Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 D-mannitol + NADP+ - ? 384520 1.1.1.67 D-fructose + NADPH + H+ low activity Thermotoga maritima JCM 10099 D-mannitol + NADP+ - r 384520 1.1.1.67 D-fructose + polyethylenimine-NH-succinyl-NADH + H+ - Saccharomyces cerevisiae D-mannitol + polyethylenimine-NH-succinyl-NAD+ - r 451094 1.1.1.67 D-fructose 1-phosphate + NADH - Leuconostoc mesenteroides ? - ? 372844 1.1.1.67 D-fructose 1-phosphate + NADH - Leuconostoc mesenteroides ATCC -9135 ? - ? 372844 1.1.1.67 D-glucitol + NAD+ - Cereibacter sphaeroides ? + NADH - ? 186924 1.1.1.67 D-glucitol + NAD+ 4% of the activity with D-mannitol Leuconostoc pseudomesenteroides ? - ? 186995 1.1.1.67 D-mannitol + NAD(P)+ - Thermotoga maritima D-fructose + NAD(P)H + H+ - r 383120 1.1.1.67 D-mannitol + NAD(P)+ - Pseudomonas fluorescens D-fructose + NAD(P)H + H+ - r 383120 1.1.1.67 D-mannitol + NAD(P)+ - Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 D-fructose + NAD(P)H + H+ - r 383120 1.1.1.67 D-mannitol + NAD+ - Sinorhizobium meliloti D-fructose + NADH + H+ - ? 186990 1.1.1.67 D-mannitol + NAD+ - Sinorhizobium meliloti D-fructose + NADH + H+ - r 186990 1.1.1.67 D-mannitol + NAD+ - Saccharomyces cerevisiae D-fructose + NADH + H+ - ? 186990 1.1.1.67 D-mannitol + NAD+ - Saccharomyces cerevisiae D-fructose + NADH + H+ - r 186990 1.1.1.67 D-mannitol + NAD+ - Mycolicibacterium smegmatis D-fructose + NADH + H+ - ? 186990 1.1.1.67 D-mannitol + NAD+ - Cereibacter sphaeroides D-fructose + NADH + H+ - ? 186990 1.1.1.67 D-mannitol + NAD+ - Cereibacter sphaeroides D-fructose + NADH + H+ - r 186990 1.1.1.67 D-mannitol + NAD+ - Bradyrhizobium japonicum D-fructose + NADH + H+ - ? 186990 1.1.1.67 D-mannitol + NAD+ - Pseudomonas fluorescens D-fructose + NADH + H+ - ? 186990 1.1.1.67 D-mannitol + NAD+ - Pseudomonas fluorescens D-fructose + NADH + H+ - r 186990 1.1.1.67 D-mannitol + NAD+ - Pseudomonas aeruginosa D-fructose + NADH + H+ - ? 186990 1.1.1.67 D-mannitol + NAD+ - Pseudomonas sp. D-fructose + NADH + H+ - ? 186990 1.1.1.67 D-mannitol + NAD+ - Leuconostoc mesenteroides D-fructose + NADH + H+ - ? 186990 1.1.1.67 D-mannitol + NAD+ - Leuconostoc mesenteroides D-fructose + NADH + H+ - r 186990 1.1.1.67 D-mannitol + NAD+ - [Brevibacterium] flavum D-fructose + NADH + H+ - ? 186990 1.1.1.67 D-mannitol + NAD+ - [Brevibacterium] flavum D-fructose + NADH + H+ - r 186990 1.1.1.67 D-mannitol + NAD+ - Levilactobacillus brevis D-fructose + NADH + H+ - ? 186990 1.1.1.67 D-mannitol + NAD+ - Levilactobacillus brevis D-fructose + NADH + H+ - r 186990 1.1.1.67 D-mannitol + NAD+ - Lactobacillus gayonii D-fructose + NADH + H+ - ? 186990 1.1.1.67 D-mannitol + NAD+ - Actinoplanes missouriensis D-fructose + NADH + H+ - ? 186990 1.1.1.67 D-mannitol + NAD+ - Burkholderia cepacia D-fructose + NADH + H+ - ? 186990 1.1.1.67 D-mannitol + NAD+ - Nocardia erythropolis D-fructose + NADH + H+ - ? 186990 1.1.1.67 D-mannitol + NAD+ - Eimeria tenella D-fructose + NADH + H+ - ? 186990 1.1.1.67 D-mannitol + NAD+ - Eimeria tenella D-fructose + NADH + H+ - r 186990 1.1.1.67 D-mannitol + NAD+ - Gluconobacter oxydans D-fructose + NADH + H+ - ? 186990 1.1.1.67 D-mannitol + NAD+ - Gluconobacter oxydans D-fructose + NADH + H+ - r 186990 1.1.1.67 D-mannitol + NAD+ - Fructilactobacillus sanfranciscensis D-fructose + NADH + H+ - r 186990 1.1.1.67 D-mannitol + NAD+ - Pseudomonas coronafaciens D-fructose + NADH + H+ - ? 186990 1.1.1.67 D-mannitol + NAD+ - Sarcina marginata D-fructose + NADH + H+ - ? 186990 1.1.1.67 D-mannitol + NAD+ - Sarcina aurantiaca D-fructose + NADH + H+ - ? 186990 1.1.1.67 D-mannitol + NAD+ - Lactobacillus pentoaceticus D-fructose + NADH + H+ - ? 186990 1.1.1.67 D-mannitol + NAD+ - Streptomyces lavendulae D-fructose + NADH + H+ - ? 186990 1.1.1.67 D-mannitol + NAD+ - Absidia glauca D-fructose + NADH + H+ - ? 186990 1.1.1.67 D-mannitol + NAD+ - Absidia glauca D-fructose + NADH + H+ - r 186990 1.1.1.67 D-mannitol + NAD+ - Fomes pinicola D-fructose + NADH + H+ - ? 186990 1.1.1.67 D-mannitol + NAD+ - Tetraselmis subcordiformis D-fructose + NADH + H+ - ? 186990 1.1.1.67 D-mannitol + NAD+ - Tetraselmis subcordiformis D-fructose + NADH + H+ - r 186990 1.1.1.67 D-mannitol + NAD+ - Leuconostoc pseudomesenteroides D-fructose + NADH + H+ - ? 186990 1.1.1.67 D-mannitol + NAD+ - Aspergillus fumigatus D-fructose + NADH + H+ - r 186990 1.1.1.67 D-mannitol + NAD+ enzyme highly specific for D-mannitol and D-fructose Levilactobacillus brevis D-fructose + NADH + H+ - r 186990 1.1.1.67 D-mannitol + NAD+ 100% activity Thermotoga maritima D-fructose + NADH + H+ - r 186990 1.1.1.67 D-mannitol + NAD+ the epsilon-NH2 group of Lys295 participates in an obligatory pH-dependent, pre-catalytic equilibrium which may control alcohol/alkoxide equilibration of the enzyme-bound D-mannitol and activates the C2 atom for subsequent catalytic oxidation by NAD+ Pseudomonas fluorescens D-fructose + NADH + H+ - ? 186990 1.1.1.67 D-mannitol + NAD+ wild-type enzyme Pseudomonas fluorescens D-fructose + NADH + H+ - r 186990 1.1.1.67 D-mannitol + NAD+ free energy profiles for the enzymatic reaction suggest that enzyme primarily acts in D-mannitol oxidation Aspergillus fumigatus D-fructose + NADH + H+ - r 186990 1.1.1.67 D-mannitol + NAD+ - Leuconostoc mesenteroides ATCC -9135 D-fructose + NADH + H+ - r 186990 1.1.1.67 D-mannitol + NAD+ - Sinorhizobium meliloti KCTC 2353 D-fructose + NADH + H+ - r 186990 1.1.1.67 D-mannitol + NAD+ - [Brevibacterium] flavum 2247 / ATCC 14067 D-fructose + NADH + H+ - ? 186990 1.1.1.67 D-mannitol + NAD+ - [Brevibacterium] flavum 2247 / ATCC 14067 D-fructose + NADH + H+ - r 186990 1.1.1.67 D-mannitol + NAD+ 100% activity Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 D-fructose + NADH + H+ - r 186990 1.1.1.67 D-mannitol + NADP+ 32% of the activity with NAD+ Leuconostoc pseudomesenteroides D-fructose + NADPH + H+ - ? 187169 1.1.1.67 D-mannitol + NADP+ M2DH is a much poorer enzyme when it employes NADP+ and NADPH as compared to NAD+ and NADH Aspergillus fumigatus D-fructose + NADPH + H+ - r 187169 1.1.1.67 D-mannitol + polyethyleneglycol-NH-succinyl-aminoethyl-NAD+ - Saccharomyces cerevisiae D-fructose + polyethyleneglycol-NH-succinyl-aminoethyl-NADH - ? 188544 1.1.1.67 D-mannitol + polyethyleneglycol-NH-succinyl-NAD+ - Saccharomyces cerevisiae D-fructose + polyethyleneglycol-NH-succinyl-NADH - ? 188542 1.1.1.67 D-mannitol + polyethylenimin-NH-succinyl-NAD+ - Saccharomyces cerevisiae D-fructose + polyethylenimin-NH-succinyl-NADH - ? 188543 1.1.1.67 D-sorbitol + NAD+ - Cereibacter sphaeroides ? - r 451093 1.1.1.67 D-tagatose + NAD(P)+ 29% relative activity compared to D-fructose Thermotoga maritima ? + NAD(P)H - ? 404915 1.1.1.67 D-tagatose + NAD+ 29% relative activity on D-tagatose compared to 100% activity on D-fructose Thermotoga maritima ? - ? 404916 1.1.1.67 D-xylose + NADH + H+ 18% activity compared to D-fructose Thermotoga maritima D-xylitol + NAD+ - r 460988 1.1.1.67 D-xylulose + NAD(P)+ 18% relative activity compared to D-fructose Thermotoga maritima ? + NAD(P)H - ? 404930 1.1.1.67 D-xylulose + NAD+ 18% relative activity on D-xylulose compared to 100% activity on D-fructose Thermotoga maritima ? - ? 404931 1.1.1.67 D-xylulose + NADH + H+ - Aspergillus fumigatus D-arabinitol + NAD+ - ? 372998 1.1.1.67 isomaltulose + NAD(P)+ - Pseudomonas fluorescens ? + NAD(P)H - ? 405513 1.1.1.67 L-sorbitol + NAD+ - Cereibacter sphaeroides L-sorbose + NADH + H+ - ? 188540 1.1.1.67 L-sorbitol + NAD+ - Bradyrhizobium japonicum L-sorbose + NADH + H+ - ? 188540 1.1.1.67 L-sorbitol + NAD+ - Leuconostoc mesenteroides L-sorbose + NADH + H+ - ? 188540 1.1.1.67 L-sorbitol + NAD+ - Aspergillus fumigatus L-sorbose + NADH + H+ - r 188540 1.1.1.67 L-sorbose + NAD(P)+ 5% relative activity compared to D-fructose Thermotoga maritima ? + NAD(P)H - ? 405653 1.1.1.67 L-sorbose + NADH + H+ - Aspergillus fumigatus L-sorbitol + NAD+ - r 405654 1.1.1.67 L-sorbose + NADH + H+ 5% relative activity on L-sorbose compared to 100% activity on D-fructose Thermotoga maritima L-sorbitol + NAD+ - ? 405654 1.1.1.67 L-sorbose + NADH + H+ 5% activity compared to D-fructose Thermotoga maritima L-sorbitol + NAD+ - r 405654 1.1.1.67 L-sorbose + NADH + H+ 5% activity compared to D-fructose Thermotoga maritima DSM 3109 L-sorbitol + NAD+ - r 405654 1.1.1.67 L-sorbose + NADH + H+ 5% activity compared to D-fructose Thermotoga maritima NBRC 100826 L-sorbitol + NAD+ - r 405654 1.1.1.67 L-sorbose + NADH + H+ 5% activity compared to D-fructose Thermotoga maritima ATCC 43589 L-sorbitol + NAD+ - r 405654 1.1.1.67 L-sorbose + NADH + H+ 5% activity compared to D-fructose Thermotoga maritima JCM 10099 L-sorbitol + NAD+ - r 405654 1.1.1.67 meso-erythritol + NAD+ - Pseudomonas fluorescens ? - ? 439739 1.1.1.67 additional information among the Escherichia coli strains, BL21 (DE3) plysS exhibits the maximum expression level of MDH (11mg/L) Pseudomonas fluorescens ? - ? 89 1.1.1.67 additional information mannitol can be produced directly from glucose in a two-step enzymatic process, using a Thermotoga neapolitana xylose isomerase mutant and TmMtDH at 60°C. No activity with glucose, xylose, threonine, arabinose, acetaldehyde, 2-butanone, sorbitol, xylitol, ethanol, or 2-butanol Thermotoga maritima ? - ? 89 1.1.1.67 additional information TM0298 shows no detectable activity on glucose, xylose, threonine, arabinose, acetaldehyde, 2-butanone, sorbitol, xylitol, ethanol, or 2-butanol Thermotoga maritima ? - ? 89 1.1.1.67 additional information slightly active with sorbitol, no activity with ribitol, arabinitol, or mesoerythritol Eimeria tenella ? - ? 89 1.1.1.67 additional information a D-arabo configuration is required for a polyol substrate to become reactive. The C2 (R) configuration as in mannitol is preferred over the C2 (S) configuration as in D-sorbitol Aspergillus fumigatus ? - ? 89 1.1.1.67 additional information no activity with glycerol, 1, 2-hexanediol, and 1,2,3-hexanetriol Pseudomonas fluorescens ? - ? 89 1.1.1.67 additional information no activity with NADP+ and NADPH Sinorhizobium meliloti ? - ? 89 1.1.1.67 additional information the enzyme shows no activity with xylitol, inositol, sorbitol, rhamnose, mannose and xylose, and with NADPH and NADP+ as cofactors Thermotoga neapolitana ? - ? 89 1.1.1.67 additional information No activity with glucose, arabinose, xylose, acetaldehyde, and 2-butanone Thermotoga maritima ? - - 89 1.1.1.67 additional information no activity with sorbitol, xylitol, mannose, rhamnose, and xylose. The enzyme from Thermotoga neapolitana is dependent on NADH Thermotoga neapolitana ? - - 89 1.1.1.67 additional information no activity with NADP+ and NADPH Sinorhizobium meliloti KCTC 2353 ? - ? 89 1.1.1.67 additional information No activity with glucose, arabinose, xylose, acetaldehyde, and 2-butanone Thermotoga maritima DSM 3109 ? - - 89 1.1.1.67 additional information No activity with glucose, arabinose, xylose, acetaldehyde, and 2-butanone Thermotoga maritima NBRC 100826 ? - - 89 1.1.1.67 additional information No activity with glucose, arabinose, xylose, acetaldehyde, and 2-butanone Thermotoga maritima ATCC 43589 ? - - 89 1.1.1.67 additional information the enzyme shows no activity with xylitol, inositol, sorbitol, rhamnose, mannose and xylose, and with NADPH and NADP+ as cofactors Thermotoga neapolitana DSM 4359 ? - ? 89 1.1.1.67 additional information mannitol can be produced directly from glucose in a two-step enzymatic process, using a Thermotoga neapolitana xylose isomerase mutant and TmMtDH at 60°C. No activity with glucose, xylose, threonine, arabinose, acetaldehyde, 2-butanone, sorbitol, xylitol, ethanol, or 2-butanol Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 ? - ? 89 1.1.1.67 additional information TM0298 shows no detectable activity on glucose, xylose, threonine, arabinose, acetaldehyde, 2-butanone, sorbitol, xylitol, ethanol, or 2-butanol Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 ? - ? 89 1.1.1.67 additional information No activity with glucose, arabinose, xylose, acetaldehyde, and 2-butanone Thermotoga maritima JCM 10099 ? - - 89 1.1.1.67 sorbitol + NAD(P)+ - Pseudomonas fluorescens ? + NAD(P)H - ? 406683 1.1.1.67 targatose + NADH + H+ 29% activity compared to D-fructose Thermotoga maritima ? + NAD+ - r 461358