1.1.1.368 1,2-cyclohexanedione + NADH + H+ - Thauera aromatica 440473 1.1.1.368 1,3-cyclopentanedione + NADH + H+ - Thauera aromatica 440478 1.1.1.368 6-hydroxycyclohex-1-ene-1-carbonyl-CoA + NAD+ - Thauera aromatica 424615 1.1.1.368 6-hydroxycyclohex-1-ene-1-carbonyl-CoA + NAD+ the enzyme participates in the central benzoyl-CoA degradation pathway of some anaerobic bacteria Thauera aromatica 424615 1.1.1.368 6-hydroxycyclohex-1-ene-1-carbonyl-CoA + NAD+ the enzyme is specific for NAD+, no activity with NADP+. The enzyme is specific for 6-hydroxycyclohex-1-ene-1-carbonyl-CoA, no activity with 2-hydroxycyclohexane-1-carbonyl-CoA Thauera aromatica 424615 1.1.1.368 additional information the enzyme exhibits a substrate specificity with highest activities on cyclic alpha- and beta-diketones including 1,2-cyclohexanedione and 1,3-cyclopentanedione, enzyme activity with 1,2-cyclohexanedione, 1,3-cyclohexanedione, and 1,3-cyclopentanedione as well as with 2,3/pentanedione, ethyl pyruvate, and cyclohexanone, but ThaADH converts neither acetophenone nor benzaldehyde which are both preferred substrates of many known zinc-dependent ADHs. The enzyme actually prefers substrates with the reactive carbonyl function being located inside a cyclohexyl or cyclopentyl structure Thauera aromatica 89