3.4.22.14 2-(acetamido)ethylene 2'-pyridyl disulfide + H2O reactivity probe, analysis of temperature-dependence of reaction kinetics Actinidia chinensis ? - ? 383632 3.4.22.14 2-(acetoxy)ethylene 2'-pyridyl disulfide + H2O reactivity probe, analysis of temperature-dependence of reaction kinetics Actinidia chinensis ? - ? 383633 3.4.22.14 2-(N'-acetyl-D-phenylalanyl)hydroxyethylene 2'-pyridyl disulfide + H2O reactivity probe, analysis of temperature-dependence of reaction kinetics Actinidia chinensis ? - ? 383635 3.4.22.14 2-(N'-acetyl-D-phenylalanylamino)ethylene 2'-pyridyl disulfide + H2O reactivity probe, analysis of temperature-dependence of reaction kinetics Actinidia chinensis ? - ? 383636 3.4.22.14 2-(N'-acetyl-L-phenylalanyl)hydroxyethylene 2'-pyridyl disulfide + H2O reactivity probe, analysis of temperature-dependence of reaction kinetics Actinidia chinensis ? - ? 383637 3.4.22.14 2-(N'-acetyl-L-phenylalanylamino)ethylene 2'-pyridyl disulfide + H2O reactivity probe, analysis of temperature-dependence of reaction kinetics Actinidia chinensis ? - ? 383638 3.4.22.14 ethyl 2-pyridyl disulfide + H2O reactivity probe, analysis of temperature-dependence of reaction kinetics Actinidia chinensis ? - ? 384684 3.4.22.14 N-acetyl-L-Phe-Gly methyl ester + H2O stopped-flow spectral analysis of increasing content of co-solvent acetonitrile on kinetics Actinidia chinensis ? - ? 385244 3.4.22.14 Insulin B-chain + H2O the enzyme cleaves most readily the carboxyl peptide bonds of those residues of the B-chain acylated by large hydrophobic residues - Val, Leu, or Phe, but not Tyr - and also the Arg22-Gly23 bond Actinidia chinensis ? - ? 15728 3.4.22.14 Collagen type I + H2O the hydrolysis of types I and II collagen by actinidin under different pH conditions is monitored by SDS-PAGE: good digestion of type I collagen at pH 7 (phosphate buffer) or pH 8.5 (Tris-HCl buffer). In citrate buffer (pH 5.5), actinidin digests more than half of the collagen type I, but in acetate buffer (pH 4), the enzyme does not hydrolyze this substrate Actinidia deliciosa ? - ? 16153