3.4.22.14	kiwellin + H2O	in vitro treatment of purified kiwellin, an allergenic protein formerly isolated from green kiwi fruit, with the protease actinidin from green kiwi fruit originates KiTH, a 20 kDa protein with 100% identity with the C-terminal region of kiwellin, and kissper, a described pore-forming peptide	Actinidia deliciosa	KiTH + kissper	-	?	402718	
3.4.22.14	additional information	actinidin compared with type II or IV collagenase isolated from intact human umbilical vein endothelial cells, hepatocytes, and thymic epithelial cells with viability more than 90%	Actinidia deliciosa	?	-	?	89	
3.4.22.14	additional information	digestibility of kiwifruit allergens, actinidin (Act d 1) and thaumatin-like protein (Act d 2), is assessed using an in vitro digestion system that approximates physiological conditions with respect to the passage of food through the stomach into the duodenum: Act d 1 precipitates in simulated gastric fluid at pH 2 and digestion of the aggregated protein proceeds slowly. The residual precipitate redissolves completely in simulated duodenal fluid at pH 6.5 and is partially digested. Act d 1 and Act d 2 display nearly unchanged IgE binding abilities	Actinidia deliciosa	?	-	?	89	
3.4.22.14	additional information	the SDS–PAGE profile of cream milk treated with actinidin reveals that both caseins, whey proteins and MFGF proteins are sharply digested by the enzyme	Actinidia deliciosa	?	-	?	89	
3.4.22.14	N-alpha-benzyloxycarbonyl-L-lysine 4-nitrophenyl ester + H2O	-	Actinidia deliciosa	N-alpha-benzyloxycarbonyl-L-lysine + 4-nitrophenol	-	?	433984	
3.4.22.14	Nalpha-benzyloxycarbonyl-L-Lys 4-nitrophenyl ester + H2O	-	Actinidia deliciosa	Nalpha-benzyloxycarbonyl-L-Lys + 4-nitrophenol	-	?	74434	
3.4.22.14	occludin + H2O	-	Actinidia deliciosa	?	-	?	393042	
3.4.22.14	sheep IgG + H2O	actinidin is ineffective on the heavy chain but has limited effects on the light chain	Actinidia deliciosa	?	-	?	434397	
3.4.22.14	casein + H2O	efficiently hydrolized in acidic pHs	Actinidia deliciosa cv. Hayward	?	-	?	15445	
3.4.22.14	Collagen + H2O	efficiently hydrolized at neutral or mild basic pH	Actinidia deliciosa cv. Hayward	?	-	?	16347