2.7.7.23 additional information - wild-type and mutant enzymes' Glc-1-P UTase activities, overview 755852 2.7.7.23 0.17 - purified enzyme, pH 7.4, 37°C 643057 2.7.7.23 0.21 - mutant enzyme G9A/K147A, pH 7.5, 80°C 755852 2.7.7.23 0.21 - mutant G9A/K147A, pH 7.5, 80°C 755852 2.7.7.23 0.43 - mutant enzyme G9A/T80A, pH 7.5, 80°C 755852 2.7.7.23 0.43 - mutant G9A/T80A, pH 7.5, 80°C 755852 2.7.7.23 0.48 - mutant enzyme T80A/Y97A, pH 7.5, 80°C 755852 2.7.7.23 0.48 - mutant T80A/Y97A, pH 7.5, 80°C 755852 2.7.7.23 0.6 - mutant enzyme T80A/Y97F, pH 7.5, 80°C 755852 2.7.7.23 0.6 - mutant T80A/Y97F, pH 7.5, 80°C 755852 2.7.7.23 1.04 - mutant enzyme G9A/Y97F, pH 7.5, 80°C 755852 2.7.7.23 1.04 - mutant G9A/Y97F, pH 7.5, 80°C 755852 2.7.7.23 1.27 - mutant enzyme Y97A/K147A, pH 7.5, 80°C 755852 2.7.7.23 1.27 - mutant Y97A/K147A, pH 7.5, 80°C 755852 2.7.7.23 1.36 - mutant enzyme T80A/K147A, pH 7.5, 80°C 755852 2.7.7.23 1.36 - mutant T80A/K147A, pH 7.5, 80°C 755852 2.7.7.23 2 - N-acetyl-alpha-D-glucosamine 1-phosphate substrate, pH 7.5, 80°C, recombinant mutant D005 protein 729696 2.7.7.23 2.2 - N-acetyl-alpha-D-glucosamine 1-phosphate substrate, pH 7.5, 80°C, recombinant wild-type ST0452 protein 729696 2.7.7.23 2.6 - N-acetyl-alpha-D-glucosamine 1-phosphate substrate, pH 7.5, 80°C, recombinant mutant D011 protein 729696 2.7.7.23 2.78 - mutant enzyme Y97F/K147A, pH 7.5, 80°C 755852 2.7.7.23 2.78 - mutant Y97F/K147A, pH 7.5, 80°C 755852 2.7.7.23 3.4 - purified enzyme, 30°C 643067 2.7.7.23 6.42 - pH 7.5, 80°C, substrates: UTP + N-acetyl-alpha-D-glucosamine 1-phosphate 662454 2.7.7.23 7.04 - mutant enzyme Y97E, pH 7.5, 80°C 755852 2.7.7.23 7.04 - mutant Y97E, pH 7.5, 80°C 755852 2.7.7.23 8.66 - purified enzyme, pH 7.5, 30°C 643061 2.7.7.23 10.3 - mutant enzyme Y97C, pH 7.5, 80°C 755852 2.7.7.23 10.3 - mutant Y97C, pH 7.5, 80°C 755852 2.7.7.23 10.31 - mutant enzyme Y97P, pH 7.5, 80°C 755852 2.7.7.23 10.31 - mutant Y97P, pH 7.5, 80°C 755852 2.7.7.23 14.9 - purified enzyme 643065 2.7.7.23 15.08 - wild-type enzyme, pH 7.5, 80°C 755852 2.7.7.23 15.1 - purified enzyme, pH 7.5, 30°C 643059 2.7.7.23 15.1 - purified enzyme, pH 8.2, 37°C 288685 2.7.7.23 16.34 - pH 7.5, 80°C, substrates: diphosphate + UDP-N-acetyl-alpha-D-glucosamine 662454 2.7.7.23 18.12 - mutant enzyme Y97G, pH 7.5, 80°C 755852 2.7.7.23 18.12 - mutant Y97G, pH 7.5, 80°C 755852 2.7.7.23 18.4 - mutant enzyme Y97F, pH 7.5, 80°C 755852 2.7.7.23 18.4 - mutant Y97F, pH 7.5, 80°C 755852 2.7.7.23 18.68 - mutant enzyme Y97I, pH 7.5, 80°C 755852 2.7.7.23 18.68 - mutant Y97I, pH 7.5, 80°C 755852 2.7.7.23 19.51 - mutant enzyme Y97M, pH 7.5, 80°C 755852 2.7.7.23 19.51 - mutant Y97M, pH 7.5, 80°C 755852 2.7.7.23 20.37 - mutant enzyme Y97K, pH 7.5, 80°C 755852 2.7.7.23 20.37 - mutant Y97K, pH 7.5, 80°C 755852 2.7.7.23 20.4 - purified enzyme 643066 2.7.7.23 21.06 - mutant enzyme Y97D, pH 7.5, 80°C 755852 2.7.7.23 21.06 - mutant Y97D, pH 7.5, 80°C 755852 2.7.7.23 22.03 - mutant enzyme Y97L, pH 7.5, 80°C 755852 2.7.7.23 22.03 - mutant Y97L, pH 7.5, 80°C 755852 2.7.7.23 27.52 - mutant enzyme Y97A, pH 7.5, 80°C 755852 2.7.7.23 27.52 - mutant Y97A, pH 7.5, 80°C 755852 2.7.7.23 29.77 - mutant enzyme Y97R, pH 7.5, 80°C 755852 2.7.7.23 29.77 - mutant Y97S, pH 7.5, 80°C 755852 2.7.7.23 31.27 - mutant enzyme Y97S, pH 7.5, 80°C 755852 2.7.7.23 31.27 - mutant Y97T, pH 7.5, 80°C 755852 2.7.7.23 34.6 - mutant enzyme Y97Q, pH 7.5, 80°C 755852 2.7.7.23 34.6 - mutant Y97Q, pH 7.5, 80°C 755852 2.7.7.23 39.3 - purified recombinant enzyme 661821 2.7.7.23 43.7 - mutant enzyme Y97H, pH 7.5, 80°C 755852 2.7.7.23 43.7 - mutant Y97H, pH 7.5, 80°C 755852 2.7.7.23 52.56 - mutant enzyme Y103V, 37°C, pH 7.5 755852 2.7.7.23 53.79 - mutant enzyme Y97T, pH 7.5, 80°C 755852 2.7.7.23 53.79 - mutant enzyme Y97V, pH 7.5, 80°C 755852 2.7.7.23 53.79 - mutant Y97V, pH 7.5, 80°C 755852 2.7.7.23 67.11 - mutant enzyme Y97N, pH 7.5, 80°C 755852 2.7.7.23 67.11 - mutant Y97N, pH 7.5, 80°C 755852 2.7.7.23 72 - purified recombinant native enzyme, pH 7.5, 37°C 662429 2.7.7.23 75.63 - wild-type enzyme, 37°C, pH 7.5 755852 2.7.7.23 93.97 - mutant enzyme Y103A, 37°C, pH 7.5 755852 2.7.7.23 99.8 - mutant enzyme Y103N, 37°C, pH 7.5 755852 2.7.7.23 330 - purified enzyme, pH 7.4, 37°C 643058 2.7.7.23 2390 - purified enzyme, pH 7.4, 37°C 643058