2.5.1.54 additional information - - 639770, 639777 2.5.1.54 additional information - metal ions are removed form the buffer solution via Chelex 100 639796 2.5.1.54 additional information - mutants, influence of aromatic amino acids on activity 639793, 639794 2.5.1.54 0.005 - partially purified isozyme synthase-trp 639760 2.5.1.54 0.008 - partially purified isoenzyme DS-Mn 639782 2.5.1.54 0.0127 - pH 7.5, 30°C 723700 2.5.1.54 0.026 - partially purified enzyme 639745 2.5.1.54 0.056 - partially purified isozyme synthase-tyr 639760 2.5.1.54 0.11 - partially purified isozyme DS-Mn, with Mn2+ 639790 2.5.1.54 0.14 - partially purified isozyme DS-Co, with Co2+ 639790 2.5.1.54 0.21 - partially purified isozyme DS-Co 639782 2.5.1.54 0.27 - partially purified Tyr-sensitive isozyme 639763 2.5.1.54 0.29 - N-terminal deletion mutant, 37°C, pH 6.4 659071 2.5.1.54 0.4 - partially purified enzyme 639741 2.5.1.54 0.8 - recombinant clone M1 in Escherichia coli strain BL21(DE3) 639795 2.5.1.54 0.81 - mutant W215A, 37°C, pH 6.4 659071 2.5.1.54 0.91 - purified enzyme 639769 2.5.1.54 0.92 - mutant I10A, 37°C, pH 6.4 659071 2.5.1.54 1.3 - partially purified enzyme 639742 2.5.1.54 1.97 - mutant N5K, 37°C, pH 6.4 659071 2.5.1.54 2.1 - purified enzyme 639746 2.5.1.54 2.3 - purified cytosolic isozyme 639765 2.5.1.54 2.37 - mutant V221A, 37°C, pH 6.4 659071 2.5.1.54 2.4 - partially purified native enzyme 639795 2.5.1.54 2.7 - wild-type, 37°C, pH 6.4 659071 2.5.1.54 2.81 - mutant L179A, 37°C, pH 6.4 659071 2.5.1.54 2.81 - mutant P150L, 37°C, pH 6.4 659071 2.5.1.54 3.07 - mutant F144A, 37°C, pH 6.4 659071 2.5.1.54 3.11 - recombinant clone M2 in Escherichia coli strain BL21(DE3) 639795 2.5.1.54 3.25 - mutant F209A, 37°C, pH 6.4 659071 2.5.1.54 3.6 - mutant L175Q, 37°C, pH 6.4 659071 2.5.1.54 4.25 - mutant L175A, 37°C, pH 6.4 659071 2.5.1.54 4.46 - mutant L175D, 37°C, pH 6.4 659071 2.5.1.54 4.5 - partially purified Phe-sensitive isozyme 639763 2.5.1.54 7.1 - purified enzyme 639758 2.5.1.54 7.7 - purified Trp-sensitive isozyme 639779 2.5.1.54 7.8 - purified enzyme 639781 2.5.1.54 8.1 - purified Trp-sensitive isozyme 639763 2.5.1.54 8.24 - purified recombinant Tyr-sensitive isozyme 639766 2.5.1.54 8.33 - purified Trp-sensitive isozyme 639779 2.5.1.54 10.75 - purified enzyme 639752 2.5.1.54 11.02 - purified Trp-sensitive isozyme 639779 2.5.1.54 13.1 - - 639768 2.5.1.54 16.4 - purified enzyme 639756 2.5.1.54 18.5 - purified enzyme 639744 2.5.1.54 18.8 - partially purified cytosolic isozyme, + 10 mM Mg2+ 639780 2.5.1.54 22 - purified recombinant Tyr-sensitive isozyme, with Cd2+ 639777 2.5.1.54 30 40 purified Tyr-sensitive isozyme, wild-type and mutant N8K 639792 2.5.1.54 37.5 - purified enzyme 639751 2.5.1.54 56 - purified enzyme 639747 2.5.1.54 67 - purified enzyme 639748 2.5.1.54 82.6 - purified enzyme 639753 2.5.1.54 139.3 - recombinant wild-type 639793 2.5.1.54 160 - wild-type 639794