1.1.1.100 additional information - the intrinsic NADPH dependent 3-ketoacyl-CoA reductase activity in the transformant Pseudomonas putida with overexpressed fabG gene increased about 1.6-fold from 0.87 to 1.39 units/mg protein, indicating successful overexpression of the fabGJ genes in Pseudomonas putida KCTC1639. 693287 1.1.1.100 0.00009 - substrate: 3-oxohexanoyl-[acyl-carrier protein], pH and temperature not specified in the publication 763418 1.1.1.100 0.0001 - substrate: 3-oxobutyryl-[acyl-carrier protein], pH and temperature not specified in the publication 763418 1.1.1.100 0.0017 0.0018 recombinant refolded enzyme 670774 1.1.1.100 0.0019 - recombinant soluble enzyme 670774 1.1.1.100 0.0032 - - 285668 1.1.1.100 0.00361 - substrate: 3-oxooctanoyl-[acyl-carrier protein], pH and temperature not specified in the publication 763418 1.1.1.100 0.00421 - substrate: 3-oxohexadecanoyl-[acyl-carrier protein], pH and temperature not specified in the publication 763418 1.1.1.100 0.00456 - substrate: 3-oxotetradecanoyl-[acyl-carrier protein], pH and temperature not specified in the publication 763418 1.1.1.100 0.005 - - 285668 1.1.1.100 0.00851 - substrate: 3-oxododecanoyl-[acyl-carrier protein], pH and temperature not specified in the publication 763418 1.1.1.100 0.0132 - - 285675 1.1.1.100 0.0168 - - 285675 1.1.1.100 0.0189 - - 285675 1.1.1.100 0.0192 - - 285675 1.1.1.100 0.0197 - - 285669 1.1.1.100 0.0197 - substrate: 3-oxodecanoyl-[acyl-carrier protein], pH and temperature not specified in the publication 763418 1.1.1.100 0.02 - with 2-octanone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 701783 1.1.1.100 0.0366 - - 285675 1.1.1.100 0.06 - mutant enzyme K173A, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C 741044 1.1.1.100 0.08 - with 4'-fluoroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 701783 1.1.1.100 0.13 - with 3'-fluoroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 701783 1.1.1.100 0.13 - with acetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 701783 1.1.1.100 0.15 - with 4'-chloroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 701783 1.1.1.100 0.16 - with propiophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 701783 1.1.1.100 0.44 - mutant enzyme R34A, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C 741044 1.1.1.100 0.44 - mutant enzyme R34A, with acetoacetyl-CoA and NADPH, pH 7.4 and 25°C 741044 1.1.1.100 0.46 - mutant enzyme K152A, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C 741044 1.1.1.100 0.5 - mutant enzyme Q126E/R168E/K169E, with acetoacetyl-CoA and NADPH, pH 7.4 and 25°C 741044 1.1.1.100 0.65 - mutant enzyme Y169A, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C 741044 1.1.1.100 0.85 - mutant enzyme Y169A, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C 741044 1.1.1.100 1.03 - with 1,1,1-trifluoroacetone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 701783 1.1.1.100 1.16 - with ethylbenzoylacetate as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 701783 1.1.1.100 1.2 - mutant enzyme K173A, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C 741044 1.1.1.100 1.3 - mutant enzyme K169E, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C 741044 1.1.1.100 1.6 - mutant enzyme R168E, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C 741044 1.1.1.100 1.7 - mutant enzyme Q126E/K169E, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C 741044 1.1.1.100 2 - mutant enzyme Y169A, with acetoacetyl-CoA and NADPH, pH 7.4 and 25°C 741044 1.1.1.100 2 - mutant R187E/R230E, acyl-carrier protein-dependent spectroscopic assay 703630 1.1.1.100 2.4 - mutant enzyme K173A, with acetoacetyl-CoA and NADPH, pH 7.4 and 25°C 741044 1.1.1.100 2.45 - with alpha-chloroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 701783 1.1.1.100 2.6 - wild type enzyme, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C 741044 1.1.1.100 3 - mutant R187A/R230A, acyl-carrier protein-dependent spectroscopic assay 703630 1.1.1.100 3.1 - mutant enzyme Y169A, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C 741044 1.1.1.100 3.5 - wild type enzyme, with acetoacetyl-CoA and NADPH, pH 7.4 and 25°C 741044 1.1.1.100 3.93 - with 2',3',4',5',6'-pentafluoroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 701783 1.1.1.100 4 - mutant R230E, acyl-carrier protein-dependent spectroscopic assay 703630 1.1.1.100 4.3 - mutant enzyme Q126E/K169E, with acetoacetyl-CoA and NADPH, pH 7.4 and 25°C 741044 1.1.1.100 4.5 - mutant enzyme K173A, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C 741044 1.1.1.100 5.4 - mutant enzyme D42A, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25°C 741044 1.1.1.100 5.5 - mutant enzyme K169E, with acetoacetyl-CoA and NADPH, pH 7.4 and 25°C 741044 1.1.1.100 6.1 - mutant R230A, acyl-carrier protein-dependent spectroscopic assay 703630 1.1.1.100 6.3 - mutant enzyme R168E, with acetoacetyl-CoA and NADPH, pH 7.4 and 25°C 741044 1.1.1.100 6.3 - mutant R187E, acyl-carrier protein-dependent spectroscopic assay 703630 1.1.1.100 6.9 - mutant R187A, acyl-carrier protein-dependent spectroscopic assay 703630 1.1.1.100 7.3 - mutant enzyme D42A, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C 741044 1.1.1.100 7.9 - mutant enzyme R168E, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C 741044 1.1.1.100 7.95 - - 285668 1.1.1.100 8.4 - mutant enzyme Q126E/R168E/K169E, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C 741044 1.1.1.100 14.8 - mutant enzyme Q126E/R168E/K169E, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C 741044 1.1.1.100 15.7 - mutant enzyme K169E, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C 741044 1.1.1.100 17.4 - mutant enzyme Q126E/K169E, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C 741044 1.1.1.100 19.3 - mutant enzyme K169E, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C 741044 1.1.1.100 20 - mutant enzyme D42A, with acetoacetyl-CoA and NADPH, pH 7.4 and 25°C 741044 1.1.1.100 20.5 - wild type enzyme, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C 741044 1.1.1.100 20.7 - mutant enzyme R34A, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C 741044 1.1.1.100 20.8 - wild type enzyme, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C 741044 1.1.1.100 21 - mutant enzyme R34A, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C 741044 1.1.1.100 21.7 - mutant enzyme R168E, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C 741044 1.1.1.100 22 - mutant enzyme K152A, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C 741044 1.1.1.100 25.5 - mutant enzyme K152A, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C 741044 1.1.1.100 31.8 - mutant enzyme Q126E/K169E, with acetoacetyl-CoA and NADH, pH 7.4 and 25°C 741044 1.1.1.100 38.29 - with ethyl 4-chloroacetoacetate as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 701783 1.1.1.100 42.6 - mutant R187E/R230E, acyl-carrier protein-independent spectroscopic assay 703630 1.1.1.100 45.6 - mutant R187A/R230A, acyl-carrier protein-independent spectroscopic assay 703630 1.1.1.100 54.2 - mutant R230E, acyl-carrier protein-independent spectroscopic assay 703630 1.1.1.100 54.5 - mutant R187E, acyl-carrier protein-independent spectroscopic assay 703630 1.1.1.100 55.3 - mutant R230A, acyl-carrier protein-independent spectroscopic assay 703630 1.1.1.100 57.9 - mutant R187A, acyl-carrier protein-independent spectroscopic assay 703630 1.1.1.100 59.8 - purified recombinant enzyme 668574 1.1.1.100 59.8 - wild-type, acyl-carrier protein-independent spectroscopic assay 703630 1.1.1.100 70.6 - wild-type, acyl-carrier protein-dependent spectroscopic assay 703630 1.1.1.100 74.6 - mutant enzyme D42A, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25°C 741044 1.1.1.100 280 - acetoacetyl-N-acetylcysteamine 285674 1.1.1.100 450 - acetoacetyl-CoA 285674 1.1.1.100 530 - acetoacteyl-ACP 285674 1.1.1.100 3839 - recombinant protein, cosubstrate NADH, pH 7.0, phosphate buffer 285679 1.1.1.100 4930 - purified enzyme 285672 1.1.1.100 9401 - purified enzyme, protein determination by amino acid analysis 285674 1.1.1.100 83140 - cosubstrate NADPH, pH 8.35, carbonate buffer 285679 1.1.1.100 136000 - purified enzyme, protein determination by calculation from absorption at 280 nm 285673 1.1.1.100 180400 - recombinant protein, cosubstrate NADPH, pH 7.0, phosphate buffer 285679