1.5.1.30 2.4 - FAD pH 7.5, 25°C 714077 1.5.1.30 0.0012 - FMN coupled oxidoreductase-luciferase assay, in 100 mM Na+/K+ phosphate buffer with 100 mM NaCl, pH 7.0, 1 microM Fre oxidoreductase, 10 microM decanal, 10 microM NADPH, 5 microM luciferase 698992 1.5.1.30 1.9 - FMN pH 7.5, 25°C 714077 1.5.1.30 1.96 - NADPH with FAD, pH 7.5, 30°C, recombinant enzyme 743224 1.5.1.30 2.59 - NADPH with FMN, pH 7.5, 30°C, recombinant enzyme 743224 1.5.1.30 12 - NADPH mutant enzyme R15A, in 20 mM N-tris[hydroxymethyl]methyl-3-aminopropanesulfonic acid buffer, pH 7.0, at 22°C 724343 1.5.1.30 25 - NADPH mutant enzyme K167A, in 20 mM N-tris[hydroxymethyl]methyl-3-aminopropanesulfonic acid buffer, pH 7.0, at 22°C 724343 1.5.1.30 150 - NADPH mutant enzyme N134A, in 20 mM N-tris[hydroxymethyl]methyl-3-aminopropanesulfonic acid buffer, pH 7.0, at 22°C 724343 1.5.1.30 570 - NADPH mutant enzyme R133A, in 20 mM N-tris[hydroxymethyl]methyl-3-aminopropanesulfonic acid buffer, pH 7.0, at 22°C 724343 1.5.1.30 2000 - NADPH mutant enzyme R225A, in 20 mM N-tris[hydroxymethyl]methyl-3-aminopropanesulfonic acid buffer, pH 7.0, at 22°C 724343