5.3.2.6 0.36 - 2-hydroxy-2,4-pentadienoate pH 7.3, 23°C, wild-type enzyme 718830 5.3.2.6 0.45 - (2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to compound 5 721792 5.3.2.6 0.45 - 2-hydroxymuconate mutant enzyme R39A, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C 726964 5.3.2.6 0.9 - 2-hydroxy-2,4-pentadienoate pH 7.3, 23°C, mutant R11A 718830 5.3.2.6 1.1 - 2-hydroxy-2,4-pentadienoate pH 7.3, 23°C 721792 5.3.2.6 1.2 - (2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate 721792 5.3.2.6 3.2 - phenylenolpyruvate pH 7.3, 24°C, recombinant hh4-OT mutant betaP1A 718874 5.3.2.6 4.2 - phenylenolpyruvate pH 7.3, 24°C, recombinant hh4-OT mutant alphaR12A 718874 5.3.2.6 4.4 - (2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to compound 5 721792 5.3.2.6 5.4 - (2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate 721792 5.3.2.6 8.3 - 2-hydroxymuconate mutant enzyme P1A, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C 726964 5.3.2.6 11 - 2-hydroxymuconate mutant 4-OT L8R/I52E, pH 7.3, 23°C 718844 5.3.2.6 11 - phenylenolpyruvate pH 7.3, 23°C 721792 5.3.2.6 13 - 2-hydroxy-2,4-pentadienoate pH 7.3, 23°C 721792 5.3.2.6 13 - 2-hydroxymuconate mutant enzyme R11A, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C 726964 5.3.2.6 16 - 5-(methyl)-2-hydroxymuconate isoform 4-oxalocrotonate tautomerase II, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C 726816 5.3.2.6 19 - 2-hydroxy-2,4-hexadienedioate pH 7.3, 23°C, mutant R39Q 718830 5.3.2.6 25 - 2-hydroxy-2,4-hexadienedioate pH 7.3, 23°C, mutant R11A 718830 5.3.2.6 27 - phenylenolpyruvate pH 7.3, 23°C 721792 5.3.2.6 42 - 2-hydroxy-2,4-hexadienedioate pH 7.3, 24°C, recombinant hh4-OT mutant alphaR12A 718874 5.3.2.6 48 - (2E)-2-hydroxy-3-phenylprop-2-enoate wild type enzyme, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C 726964 5.3.2.6 70 - phenylenolpyruvate pH 7.3, 24°C, recombinant hh4-OT mutant betaR39A 718874 5.3.2.6 86 - phenylenolpyruvate pH 7.3, 24°C, recombinant hh4-OT mutant betaR11A 718874 5.3.2.6 97 - 2-hydroxy-2,4-hexadienedioate pH 7.3, 23°C, mutant R39A 718830 5.3.2.6 110 - phenylenolpyruvate pH 7.3, 24°C, recombinant wild-type hh4-OT 718874 5.3.2.6 190 - 2-hydroxy-2,4-hexadienedioate pH 7.3, 24°C, recombinant hh4-OT mutant alphaR40A 718874 5.3.2.6 290 - phenylenolpyruvate pH 7.3, 24°C, recombinant hh4-OT mutant alphaR40A 718874 5.3.2.6 310 - 2-hydroxymuconate isoform 4-oxalocrotonate tautomerase II, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C 726816 5.3.2.6 460 - 2-hydroxymuconate mutant enzyme R61A, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C 726964 5.3.2.6 520 - 2-hydroxymuconate mutant 4-OT I52E, pH 7.3, 23°C 718844 5.3.2.6 1000 - 5-(methyl)-2-hydroxymuconate isoform 4-oxalocrotonate tautomerase I, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C 726816 5.3.2.6 2100 - 2-hydroxy-2,4-hexadienedioate pH 7.3, 24°C, recombinant hh4-OT mutant betaP1A 718874 5.3.2.6 3600 - 2-hydroxymuconate mutant 4-OT L8R, pH 7.3, 23°C 718844 5.3.2.6 3600 - 2-hydroxymuconate wild type enzyme, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C 726964 5.3.2.6 5400 - 2-hydroxy-2,4-hexadienedioate pH 7.3, 24°C, recombinant hh4-OT mutant betaR39A 718874 5.3.2.6 12000 - 2-hydroxy-2,4-hexadienedioate pH 7.3, 23°C, mutant R11A/R39A 718830 5.3.2.6 19000 - 2-hydroxy-2,4-hexadienedioate pH 7.3, 23°C, wild-type enzyme 718830 5.3.2.6 19000 - 2-hydroxymuconate wild-type 4-OT, pH 7.3, 23°C 718844 5.3.2.6 26600 - (2Z,4E)-2-hydroxyhexa-2,4-dienedioate at pH 7.3 and 22°C 747782 5.3.2.6 29000 - 2-oxo-4(E)-hexenedioate L-4OT enantiomer, pH 7.3, 30°C 719636 5.3.2.6 32000 - 2-oxo-4(E)-hexenedioate D-4OT enantiomer, pH 7.3, 30°C 719636 5.3.2.6 33000 - 2-hydroxymuconate isoform 4-oxalocrotonate tautomerase I, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C 726816 5.3.2.6 43000 - 2-hydroxy-2,4-hexadienedioate pH 7.3, 24°C, recombinant wild-type hh4-OT 718874 5.3.2.6 51000 - 2-hydroxy-2,4-hexadienedioate pH 7.3, 24°C, recombinant hh4-OT mutant betaR11A 718874 5.3.2.6 957000 - 2-hydroxymuconate pH 7.3, 30°C 719633 5.3.2.6 1520000 - 2-oxo-3-hexenedioate pH 7.3, 30°C 719633