3.1.3.13 additional information the erythrocytic enzyme is trifunctional exhibiting 2,3-diphosphoglycerate phosphatase, synthase, and mutase activities, overview 713630 3.1.3.13 physiological function the trifunctional enzyme regulates the level of 2,3-bisphosphoglycerate in red blood cells, the 2,3-bisphosphoglycerate phosphatase activity is responsible for rapid decline of 2,3-bisphosphoglycerate from storage in erythrocytes following fall of pH to limit the release of hemoglobin to blood 713630