1.13.11.68	evolution	occurrence of duplication in CCD4 genes that evolved into two new genes CCD7 and CCD8, EC 1.13.11.70. The site-specific profile and coefficient of type-I functional divergences reveals critical amino acid residues, leading to subgroup-specific functional evolution after their phylogenetic diversification	745435	
1.13.11.68	evolution	Zea mays ZmCCD7 and Zea mays subsp. parviglumis ZpCCD7 have the same coding sequence, indicating negative selection of the CCD7 gene over domestication from Zea mays subsp. parviglumis to Zea mays. ZmCCD7/ZpCCD7 encodes a carotenoid cleavage dioxygenase mediating strigolactone biosynthesis in maize and its ancestor	-, 746156	
1.13.11.68	malfunction	gene silencing of CCD7 in Phelipanche aegyptiaca by tobacco rattle virus system retards the parasite development on the host. Transient knockdown of PaCCD7 inhibits tubercle development and the infestation process in host plants. The number of parasite tubercles attached to the roots of host plants treated with TRV:PaCCD7, TRV:PaCCD8, or a mixture of TRV:PaCCD7 and TRV:PaCCD8 is significantly reduced by 95% as compared to control plants	746148	
1.13.11.68	malfunction	suppression of shoot branching in the Arabidopsis thaliana Col-0 ccd7 max3 mutant by transformation of ZmCCD7/ZpCCD7. Knockout or knockdown of these CCD7 results in numerous shoot branches by blocking or reducing strigolactone production	-, 746156	
1.13.11.68	malfunction	tomato plants expressing a SlCCD7 antisense construct display greatly increased branching. A metabolomic screen is conducted. With the exception of a reduction of stem amino acid content in the transgenic lines, no major changes are observed. Targeted analysis of the same plants reveal significantly decreased levels of strigolactone. There are no significant changes in root carotenoids, indicating that relatively little substrate is required to produce the bioactive strigolactones. The germination rate of Orobanche ramosa seeds is reduced by up to 90% on application of extract from the SlCCD7 antisense lines, compared with the wild type. Upon mycorrhizal colonization, C13 cyclohexenone and C14 mycorradicin apocarotenoid levels are greatly reduced in the roots of the antisense lines, implicating SlCCD7 in their biosynthesis	728478	
1.13.11.68	metabolism	biosynthesis of strigolactones requires the action of two CCD enzymes, CCD7 and CCD8 (EC 1.13.11.70), which act sequentially on 9-cis-beta-carotene, strigolactone biosynthesis pathway from all-trans-beta-carotene to ent-2'-epi-5-deoxystrigol	744876	
1.13.11.68	additional information	in silico analysis, structure homology modeling, molecular modeling, dynamic simulation and structure comparisons of Arabidopsis thaliana carotenoid cleavage dioxygenases, overview	745435	
1.13.11.68	additional information	the biochemical basis of the shoot branching phenotype is not due to inhibition of enzyme CCD7, but of enzyme CCD8, EC 1.13.11.70	744876	
1.13.11.68	physiological function	biosynthesis of strigolactones requires the action of two CCD enzymes, CCD7 and CCD8 (EC 1.13.11.70), which act sequentially on 9-cis-beta-carotene	744876	
1.13.11.68	physiological function	CCD7 enzymes are highly stereospecific, cleaving only 9-cis-configured substrates, such as 9-cis-beta-carotene. The strigolactone biosynthetic enzyme AtCCD7 converts 9-cis-configured acyclic carotenes, such as 9-cis-zeta-carotene, 9'-cis-neurosporene, and 9-cis-lycopene, yielding 9-cis-configured products and indicating that AtCCD7, rather than AtCCD4, is the candidate for forming acyclic retrograde signals that are thought to derive from the cleavage of poly-cis-configured carotene desaturation intermediates	745469