3.4.22.B5	additional information	recombinant enzyme mutant CPB2.8DEKTACTE structure homology modeling using 1.75 A resolution crystal structure of cruzain complexed with the irreversible fluoromethyl ketone inhibitor Mor-Leu-Hpq was used as a template, PDB ID 1EWP, overview	732753	
3.4.22.B5	physiological function	amastigotes and promastigotes release the C-terminal fragment Cyspep into the extracellular environment, but only promastigotes release this polypeptide as Pro-CPB	-, 754984	
3.4.22.B5	physiological function	CPB is an important virulence factor, it inhibits lymphocytes Th1 and/or promotes Th2 responses either through proteolytic activity or through epitopes derived from its COOH-terminal extension. Some peptides from the CPB COOH-terminal extension influence host immune responses in the murine infection, thus helping Leishmania survival	-, 717441	
3.4.22.B5	physiological function	in a CPB-deficient mutant, both SNARE proteins VAMP3 and VAMP8 are down-modulated in a CPB-dependent manner. Expression of the virulence-associated GPI-anchored metalloprotease GP63 is inhibited in the absence of CPB. Expression of GP63 in the CPB-deficient mutant is sufficient to down-modulate VAMP3 and VAMP8. Episomal expression of GP63 enables the CPB-deficient mutant to establish infection in macrophages	-, 755206	
3.4.22.B5	physiological function	The proteolytic activity of Cbp proteins exhibits a gradual increase during the parasite's differentiation with low levels in samples of logarithmic promastigotes phase (3.2 mmol per min and mg protein) to a peak of activity after 72 h of incubation at 32°C (4.2 mmol per min and mg protein) followed by a subsequent decrease of 68 % of peak activity levels after 96 h of incubation at 32°C (2.8 mmol per min and mg protein)	-, 752856	
3.4.22.B5	physiological function	upon processing from its zymogen form, a release of the immunomodulatory CPB C-terminal extension (cyspep) into the cytoplasm of the macrophage takes place. Molecular models show a prevalence of beta-sheets in the cyspep protein. The formation of new strands, along with a rapid disruption of helical content, is observed	754450