2.6.1.98 evolution WbpE is a member of the broad class of fold type I aspartate-aminotransferase enzymes, which harness the powerful electron-sink properties of PLP to carry out a wide variety of transformations, including transaminations, eliminations, decarboxylations, and racemizations. The general mechanism of this class of enzymes has been worked out in great detail, and is divided into two discrete half reactions that cycle between the PMP and PLP forms of the cofactor, overview 707534 2.6.1.98 malfunction B-band LPS production is restored to Pseudomonas aeruginosa knockout mutants when the relevant Bordetella pertussis genes are supplied in trans 692874 2.6.1.98 metabolism the biosynthetic pathway begins with WbpA, catalyzing the C6-oxidation of UDP-GlcNAc to give the corresponding UDP-N-acetyl-D-glucosaminuronic acid. The C3-dehydrogenase WbpB, aminotransferase WbpE, and acetyltransferase WbpD sequentially convert UDP-GlcNAcA into UDP-2,3-diacetamido-2,3-dideoxy-D-glucuronic acid. Finally, the C2-epimerase WbpI modifies UDP-GlcNAc(3NAc)A to give the final UDP-ManNAc(3NAc)A 718852 2.6.1.98 metabolism the biosynthetic pathway of 2,3-diacetamido-2,3-dideoxy-D-mannuronic acid production as part of the B-band LPS production requires the five enzymes WbpA, WbpB, WbpE, WbpD, and WbpI 692874 2.6.1.98 metabolism the central carbohydrate of the Pseudomonas aeruginosa PAO1 (O5) B-band O-antigen, UDP-2,3-diacetamido-2,3-dideoxy-D-mannuronic acid or ManNAc(3NAc)A, is critical for virulence and is produced in a stepwise manner by the five enzymes in the Wbp pathway, WbpA, WbpB, WbpE, WbpD and WbpI, overview 707534 2.6.1.98 metabolism the enzyme catalyzes the third step in the biosynthesis of UDP-2,3-diacetamido-2,3-dideoxy-D-mannuronic acid -, 760097 2.6.1.98 metabolism WbpE substrate 2-acetamido-2-deoxy-D-ribohex-3-uluronic acid is synthesized by WbpB 709007 2.6.1.98 additional information analysis of external aldimine structures of WpbE solved in the presence of either dTDP-3-amino-3,6-dideoxy-D-galactose or dTDP-3-amino-3,6-dideoxy-D-glucose -, 760097 2.6.1.98 additional information Bordetella pertussis genes wbpO1629 and wbpO3150 complement a wbpA knockout of Pseudomonas aeruginosa. B-band LPS production is restored to Pseudomonas aeruginosa knockout mutants when the relevant Bordetella pertussis genes are supplied in trans 692874 2.6.1.98 additional information WbpE nucleotide sugar-binding site structure, overview 707534 2.6.1.98 physiological function a strain lacking PorR activity expresses the conventional O-antigen of orphyromonas gingivalis strain W50 (O-LPS), but lacks A-LPS, i.e. a different O-antigen consisting of an anionic polysaccharide (APS) repeat unit. PorE is a homolog of WbpE -, 737244 2.6.1.98 physiological function WbpE from Pseudomonas aeruginosa catalyzes the amination of UDP-3-keto-2-acetamido-2-deoxy-D-glucuronic acid (UDP-3-keto-GlcNAcA) representing the third step in the biosynthesis of UDP-2,3-diacetamido-2,3-dideoxy-D-mannuronic acid -, 760097 2.6.1.98 physiological function WbpE, a nucleotide sugar aminotransferase involved in O-antigen assembly, is a pyridoxal 5'-phosphate-dependent aminotransferse responsible for the conversion of UDP-2-acetamido-2-deoxy-3-oxo-D-glucuronic acid and L-glutamate to UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid and 2-oxoglutarate, respectively 707534