2.3.1.54	malfunction	an enzyme-deletion mutant reveals a 60% decrease in invasion efficiency when compared to the wild type	-, 757504	
2.3.1.54	malfunction	mutation of either pflB, which codes for PFL, or pflA, which codes for pyruvate formate lyase activating enzyme, results in abrogation of mixed acid fermentation on galactose, and leads to a decrease in pneumococcal virulence	746477	
2.3.1.54	metabolism	anaerobic fermentation pathway yielding 3 ATP per glucose, pyruvate channeling to fermentation product, lactate dehydrogenase and pyruvate dehydrogenase are alternative pyruvate channeling enzymes during fermentation	701826	
2.3.1.54	metabolism	anaerobic glucose metabolism, direct link between fermentative metabolism and virulence in infected mice	704002	
2.3.1.54	metabolism	multiple regulators control the transcription of pflA and pflB, some of these regulators are induced by galactose, their control over pflA and pflB is influenced by sodium formate, they exert regulatory influence on each other, and are required for pneumococcal colonization and virulence. Transcriptional profile of mutant DELTApflB compared to the wild-type D39 strain, expression of seven genes annotated as transcriptional regulators is either significantly up or downregulated in the mutant, overview. CcpA, GlnR, and GntR interact with the putative promoters of pflA and pflB, impact of formate on binding affinity of CcpA and GlnR	746477	
2.3.1.54	metabolism	NADH generated from anaerobic glycerol metabolism in the absence of fumarate is oxidized through the pyruvate formate-lyase-ethanol fermentation pathway. Thus, the enzyme is essential to avoid the accumulation of excess NADH during fumarate-independent anaerobic glycerol metabolism	-, 736361	
2.3.1.54	metabolism	the enzyme can serve as a sole route for microbial growth on acetate and formate	756036	
2.3.1.54	metabolism	the enzyme catalyzes the conversion of pyruvate and CoA to formate and acetyl-CoA during anaerobic glucose metabolism	757586	
2.3.1.54	metabolism	the enzyme takes part in the metabolic pathway of pyruvate to ethanol in Thermoanaerobacterium saccharolyticum	-, 735819	
2.3.1.54	physiological function	anaerobic alkane degradation in Archaeoglobus fulgidus may involve the gene pflD in alkane activation through addition to fumarate	736285	
2.3.1.54	physiological function	enzyme deletion mutant displays pleiotropic effects. In the mutant, no formate is produced, glucose consumption is delayed, and ethanol production is decreased, whereas acetate and lactate production are unaffected. All metabolic alterations can be restored by addition of formate or complementation of the mutant. In compensation reactions, serine and threonine are consumed better by the mutant than by the wild-type. The mutant displays reduced production of formylated peptides compared to the parental strain. Arginine consumption and arc operon transcription are increased in the mutant. Enzyme plays a significant role in the anaerobic layer of a biofilm	719737	
2.3.1.54	physiological function	enzyme pyruvate formate-lyase is responsible for intracellular formate generation in enterobacteria and other microbes	736638	
2.3.1.54	physiological function	pyruvate formate lyase (PFL) forms an alternative pathway for acetyl-CoA biosynthesis. Pyruvate formate lyase is characterized as an enzyme functional at anaerobic conditions, since the radical in the enzyme's active form is sensitive to oxygen. PFL pathway can be functional at aerobic growth conditions in yeast when coexpressed with appropriate electron donors	744943	
2.3.1.54	physiological function	pyruvate formate lyase (PFL), converting pyruvate to formate and acetyl-CoA, is the key enzyme for mixed acid fermentation. Active pyruvate formate lyase (PFL) synthesis is finely tuned, and feedback inhibition and activation involving the pyruvate formate lyase activating enzyme, are detected, analysis of the transcriptional regulation of gene pflA, overview. The pyruvate formate lyase activating enzyme (PFL-AE) is responsible for posttranslational activation of inactive PFL	746477	
2.3.1.54	physiological function	pyruvate formate-lyase (PFL) supplies the citric acid cycle with acetyl-CoA during anaerobic glycolysis by catalyzing the formation of acetyl-CoA and formate from CoA and pyruvate, and is a central enzyme in anaerobic metabolism of Escherichia coli and other facultative anaerobes. PFL is a glycyl radical enzyme, which play key roles in anaerobic metabolism in microbes, including the reduction of ribonucleotides to deoxyribonucleotides, synthesis of benzylsuccinate, and conversion of choline to trimethylamine	745316	
2.3.1.54	physiological function	the enzyme is involved in the invasion of intestinal cells	-, 757504	
2.3.1.54	physiological function	the physiological role of pyruvate formate-lyase is supplying C1 units for biosyntheses	-, 735819