1.14.19.39	evolution	in plants, membrane-bound fatty acid desaturases are present in the plastid and the endoplasmic reticulum. The electron donors for plastid desaturases are typically ferredoxins, while endoplasmic reticulum enzymes use cytochrome b5 (Cytb5)	745739	
1.14.19.39	evolution	the microsomal DELTA12-fatty acid desaturase (FAD2) is located in the endoplasmic reticulum and uses phosphatidylcholine as acyl substrate and NADH, NADH-cytochrome b5 reductase, and cytochrome b5 as electron donors. This reaction involves concomitant reduction of molecular oxygen to water. In contrast, the plastidial DELTA12-fatty acid desaturase (FAD6) is located in the chloroplast and uses primarily glycolipids as acyl carriers and NAD(P)H, ferredoxin-NAD(P) reductase, and ferredoxin as electron donors	728074	
1.14.19.39	metabolism	the bifunctional desaturase and the acetylenase provide the enzymatic activities required to drive oleate through linoleate to crepenynate and the conjugated enyne (14Z)-dehydrocrepenynate, the branchpoint precursors to a major class of acetylenic natural products, biosynthesis of polyacetylenic metabolites in Basidiomycetes, overview	715539	
1.14.19.39	additional information	the acetylenase gene, identified from the fungus, is phylogenetically distinct from known plant and fungal desaturases. The enzyme contains positionally conserved histidine box motifs that are believed to bind two active-site iron atoms and are critical to enzyme function	715539	
1.14.19.39	additional information	the isozyme contains the three histidine clusters typical of the catalytic domains of DELTA12-desaturase enzymes	728074	
1.14.19.39	physiological function	in plants, seed-specific delta-12 fatty acid desaturase 2 (FAD2) is responsible for the high content of linoleic acid by inserting a double bond at the delta-12 (omega-6) position of oleic acid. FAD2-2 genes encoding isoforms of the large and functionally diverse FAD2 gene family are detected in developing seeds suggesting their major roles in storage oil desaturation in seed	745739	
1.14.19.39	physiological function	to produce the accumulated natural product dehydrocrepenynic acid, chanterelle must have both FAD2/3 desaturase, EC 1.14.19.6. and acetylenase activities, production of the trans DELTA12 double bond by H6Cf0807	715539