3.1.4.54	down	capsaicin treatment overnight downregulates NAPE-PLD expression in capsaicin-sensitive cells by 70% at 0.01 mM	
3.1.4.54	down	the pro-inflammatory bacterial endotoxin, lipopolysaccharide, decreases palmitoylethanolamide biosynthesis in RAW264.7 macrophages by suppressing the transcription of NAPE-PLD, which catalyzes the production of palmitoylethanolamide and other lipid amides. Liopolysaccharide treatment reduces acetylation of histone proteins bound to the NAPE-PLD promoter, an effect that is blocked by the histone deacetylase inhibitor richostatin A. The transcription factor Sp1 is involved in regulating baseline NAPE-PLD expression, but not in the transcriptional suppression induced by lipopolysaccharide. The down-regulation of NAPE-PLD expression may be functionally important because mutant NAPE-PLD-null mice, in which this regulatory process is defective, are unable to mount a normal inflammatory reaction in response to carrageenan	
3.1.4.54	down	ethanol downregulates microglial NAPE-PLD expression by activating cAMP/PKA and ERK1/2	
3.1.4.54	additional information	Nape-pld is regulated by ovarian steroid hormones via their nuclear receptors	
3.1.4.54	additional information	the enzyme appears to be constitutively active