2.7.8.24 CDP-diacylglycerol + choline = CMP + phosphatidylcholine Requires divalent cations, with Mn2+ being more effective than Mg2+ - 2.7.8.24 CDP-diacylglycerol + choline = CMP + phosphatidylcholine the enzyme reaction follows a reversible two-step reaction: in a first choline- and manganese-independent reaction, CDP-diacylglycerol is hydrolyzed releasing a CMP molecule. The resulting phosphatidyl intermediate reacts with choline in a second manganese-dependent step to form phosphatidylcholine -, 738208