3.4.22.55 strict requirement for an Asp residue at P1, with Asp316 being essential for proteolytic activity and has a preferred cleavage sequence of Val-Asp-Val-Ala-Asp-/- - - 3.4.22.55 strict requirement for an Asp residue at P1, with Asp316 being essential for proteolytic activity and has a preferred cleavage sequence of Val-Asp-Val-Ala-Asp-/- catalysis is mediated by a mechanism involving a catalytic dyad composed of a cysteine residue as the catalytic nucleophile and a histidine residue 701085